02145nas a2200409 4500008004100000245007300041210006900114260001200183490000700195520089800202653005701100653003001157653002601187653001701213653002401230653002001254653001001274100002001284700002101304700001701325700002201342700001801364700002101382700001601403700002101419700001501440700001801455700002301473700001801496700002601514700002201540700002301562700002201585700002501607700001901632856008401651 2024 eng d00aBiogeographic survey of soil bacterial communities across Antarctica0 aBiogeographic survey of soil bacterial communities across Antarc c01/20240 v123 a

Antarctica and its unique biodiversity are increasingly at risk from the effects of global climate change and other human influences. A significant recent element underpinning strategies for Antarctic conservation has been the development of a system of Antarctic Conservation Biogeographic Regions (ACBRs). The datasets supporting this classification are, however, dominated by eukaryotic taxa, with contributions from the bacterial domain restricted to Actinomycetota and Cyanobacteriota. Nevertheless, the ice-free areas of the Antarctic continent and the sub-Antarctic islands are dominated in terms of diversity by bacteria. Our study aims to generate a comprehensive phylogenetic dataset of Antarctic bacteria with wide geographical coverage on the continent and sub-Antarctic islands, to investigate whether bacterial diversity and distribution is reflected in the current ACBRs.

10aAntarctic Conservation Biogeographic Regions (ACBRs)10aAntarctic soil microbiome10abioclimatic variables10abiogeography10amicrobial diversity10aregionalization10asoils1 aVarliero, Gilda1 aLebre, Pedro, H.1 aAdams, Byron1 aChown, Steven, L.1 aConvey, Peter1 aDennis, Paul, G.1 aFan, Dandan1 aFerrari, Belinda1 aFrey, Beat1 aHogg, Ian, D.1 aHopkins, David, W.1 aKong, Weidong1 aMakhalanyane, Thulani1 aMatcher, Gwynneth1 aNewsham, Kevin, K.1 aStevens, Mark, I.1 aWeigh, Katherine, V.1 aCowan, Don, A. uhttps://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-023-01719-301464nas a2200181 4500008004100000022001400041245014100055210006900196260001200265520082200277653002101099653002001120653002301140653001801163653001001181100002901191856006201220 2024 eng d a0028-646X00aLong days and long nights: An integrative study reveals survival strategies of an Antarctic diatom during the cold and dark polar winter0 aLong days and long nights An integrative study reveals survival  c01/20243 a

The vast majority of photosynthetic organisms on Earth have evolved under a circadian cycle, with many cellular processes being regulated by the predictable patterns of day and night. Polar algal species living in Arctic and Antarctic aquatic habitats are faced with bizarre light environments of continuous light during the short summer months and 24-h darkness during the winter, that is one long day transitioning into one long, cold night. It is well known that polar phytoplankton survive the winter and return in the summer to form blooms. In an article recently published in New Phytologist, Joli et al. (2023, doi: 10.1111/nph.19387) use an integrative approach to dissect how an Antarctic marine diatom not only survives the long, dark polar winter, but also recovers rapidly upon the onset of summer.

10aaquatic habitats10acircadian cycle10alight environments10aphytoplankton10apolar1 aMorgan-Kiss, Rachael, M. uhttps://nph.onlinelibrary.wiley.com/doi/10.1111/nph.1953602587nas a2200313 4500008004100000022001400041245012700055210006900182260001200251300001100263520145800274653001701732653001401749653000801763653001901771653001501790100002301805700001801828700001501846700002401861700002501885700003301910700002901943700001701972700002201989700002102011700002102032856022002053 2024 eng d a0954-102000aMcMurdo Dry Valley lake edge ‘moats’: The ecological intersection between terrestrial and aquatic polar desert habitat0 aMcMurdo Dry Valley lake edge moats The ecological intersection b c04/2024 a1 - 173 a

Aquatic ecosystems - lakes, ponds and streams - are hotspots of biodiversity in the cold and arid environment of Continental Antarctica. Environmental change is expected to increasingly alter Antarctic aquatic ecosystems and modify the physical characteristics and interactions within the habitats that they support. Here, we describe physical and biological features of the peripheral ‘moat’ of a closed-basin Antarctic lake. These moats mediate connectivity amongst streams, lake and soils. We highlight the cyclical moat transition from a frozen winter state to an active open-water summer system, through refreeze as winter returns. Summer melting begins at the lakebed, initially creating an ice-constrained lens of liquid water in November, which swiftly progresses upwards, creating open water in December. Conversely, freezing progresses slowly from the water surface downwards, with water at 1 m bottom depth remaining liquid until May. Moats support productive, diverse benthic communities that are taxonomically distinct from those under the adjacent permanent lake ice. We show how ion ratios suggest that summer exchange occurs amongst moats, streams, soils and sub-ice lake water, perhaps facilitated by within-moat density-driven convection. Moats occupy a small but dynamic area of lake habitat, are disproportionately affected by recent lake-level rises and may thus be particularly vulnerable to hydrological change.

10aconnectivity10aecosystem10aice10amicrobial mats10atransition1 aStone, Michael, S.1 aDevlin, Shawn1 aHawes, Ian1 aWelch, Kathleen, A.1 aGooseff, Michael, N.1 aTakacs-Vesbach, Cristina, D.1 aMorgan-Kiss, Rachael, M.1 aAdams, Byron1 aBarrett, John, E.1 aPriscu, John, C.1 aDoran, Peter, T. uhttps://www.cambridge.org/core/journals/antarctic-science/article/mcmurdo-dry-valley-lake-edge-moats-the-ecological-intersection-between-terrestrial-and-aquatic-polar-desert-habitats/31D94DD51E651603482A3AE6E8A52A5702559nas a2200301 4500008004100000245008000041210006900121260001200190300001200202490000700214520166000221653001501881653001601896653002901912653001801941653001201959100002901971700001802000700002202018700002002040700002202060700001202082700001602094700002602110700002102136700003302157856006702190 2024 eng d00aSentinel protist taxa of the McMurdo Dry Valley lakes, Antarctica: A review0 aSentinel protist taxa of the McMurdo Dry Valley lakes Antarctica c03/2024 a13234720 v123 a

High-latitude meromictic lakes such as those in the Antarctic McMurdo Dry Valleys (MDV) harbor aquatic ecosystems dominated by the microbial loop. Within this habitat, which is limited year-round by light and nutrients, protists, or single celled eukaryotes, play outsized roles in the food web as the dominant primary producers and the apex predators. Thus, the MDV lake ecosystem represents an ideal system to study the role of sentinel protist taxa in carbon and nutrient cycling. The perennially ice-covered lakes are part of the McMurdo Long Term Ecological Research (McM LTER; mcmlter.org) established in 1993. In this review we will highlight the diversity and trophic roles of the MDV lake protist community and compare environmental factors driving spatiotemporal patterns in key protist taxa in two lakes within the McM LTER, Lakes Bonney and Fryxell. We will then discuss lessons learned from manipulated experiments on the impact of current and future climate-driven environmental change on sensitive protist taxa. Last, we will integrate knowledge gained from 25 years of lab-controlled experiments on key photosynthetic protists to extend our understanding of the function of these extremophiles within the MDV aquatic food webs. Our research group has studied the distribution and function of the MDV microbial community for nearly two decades, training the next generation of scientists to tackle future problems of these globally significant microbes. This review article will also highlight early career scientists who have contributed to this body of work and represent the future of scientific understanding in the Anthropocene.

10aAntarctica10aDisturbance10aMcMurdo Dry Valley lakes10aphytoplankton10aprotist1 aMorgan-Kiss, Rachael, M.1 aPopson, Devon1 aPereira, Rochelle1 aDolhi-Binder, J1 aTeufel, Amber, G.1 aLi, Wei1 aKalra, Isha1 aSherwell, Shasten, S.1 aReynebeau, Emily1 aTakacs-Vesbach, Cristina, D. uhttps://www.frontiersin.org/articles/10.3389/fevo.2024.132347202505nas a2200325 4500008004100000022001400041245012700055210006900182260001200251300001800263490000800281520146500289653001701754653003701771653002401808653002401832100002701856700002101883700002301904700002201927700002201949700002501971700002001996700002202016700002202038700002202060700002202082700001402104856006102118 2023 eng d a2169-900300aCauses and characteristics of electrical resistivity variability in shallow (<4 m) soils in Taylor Valley, East Antarctica0 aCauses and characteristics of electrical resistivity variability c02/2023 ae2022JF0066960 v1283 a

Airborne electromagnetic surveys collected in December 2011 and November 2018 and three soil sampling transects were used to analyze the spatial heterogeneity of shallow (<4 m) soil properties in lower Taylor Valley (TV), East Antarctica. Soil resistivities from 2011 to 2018 ranged from ∼33 Ωm to ∼3,500 Ωm with 200 Ωm assigned as an upper boundary for brine-saturated sediments. Elevations below ∼50 m above sea level (masl) typically exhibit the lowest resistivities with resistivity increasing at high elevations on steeper slopes. Soil water content was empirically estimated from electrical resistivities using Archie's Law and range from ∼<1% to ∼68% by volume. An increase in silt- and clay-sized particles at low elevations increases soil porosity but decreases hydraulic conductivity, promoting greater residence times of soil water at low elevations near Lake Fryxell. Soil resistivity variability between 2011 and 2018 shows soils at different stages of soil freeze-thaw cycles, which are caused predominantly by solar warming of soils as opposed to air temperature. This study furthers the understanding of the hydrogeologic structure of the shallow subsurface in TV and identifies locations of soils that are potentially prone to greater rates of thaw and resulting ecosystem homogenization of soil properties from projected increases in hydrological connectivity across the region over the coming decades.

10aactive layer10aairborne electromagnetic surveys10aMcMurdo Dry Valleys10apermafrost dynamics1 aGutterman, William, S.1 aDoran, Peter, T.1 aVirginia, Ross, A.1 aBarrett, John, E.1 aMyers, Krista, F.1 aTulaczyk, Slawek, M.1 aFoley, Neil, T.1 aMikucki, Jill, A.1 aDugan, Hilary, A.1 aGrombacher, Denys1 aBording, Thue, S.1 aAuken, E. uhttps://onlinelibrary.wiley.com/doi/10.1029/2022JF00669603072nas a2200253 4500008004100000022001400041245011200055210006900167260001200236490000700248520227400255653001902529653001702548653002102565653002002586653001902606653001502625100002202640700002402662700002502686700002402711700002602735856005702761 2023 eng d a2161-954900aDifferentiating physical and biological storage of nitrogen along an intermittent Antarctic stream corridor0 aDifferentiating physical and biological storage of nitrogen alon c09/20230 v423 a

In many temperate streams, biological uptake of N acts to attenuate the transport of excess N from allochthonous anthropogenic imports. Relatively few studies have determined how this N uptake relates to the magnitude of physical vs. biological N storage in the stream corridor, especially for intermittent systems where allochthonous N imports are often low and N transport may only occur during brief periods of streamflow. Glacial meltwater streams in the McMurdo Dry Valleys of Antarctica provide an excellent setting to quantify autochthonous N cycling and storage processes supported by abundant algal mats and well-connected hyporheic zones. We combined historic point-scale sediment and periphyton sample datasets with remote sensing-based modeling of periphyton coverage to estimate how much N was stored in periphyton biomass and the hyporheic zone of a 5-km long McMurdo Dry Valley stream corridor (>100,000 m2). We contextualized these N storage calculations by estimating the magnitude of annual N imports to and exports from the stream corridor based on >2 decades of streamflow and surface water data, source glacier ice cores and meltwater data, and past studies of local aeolian deposition and biological N fixation rates. We found that in this highly oligotrophic system, stream corridor-scale N storage was ~1000x that of total annual N import or export fluxes. More than 90% of this temporarily stored N was autochthonous organic matter in the shallow (<10 cm) hyporheic zone, which acts as a reservoir that sustains N availability in the water column. Despite its location in a polar desert devoid of higher-order vegetation, area-normalized N storage (~40 g N/m2) was greater than that reported for streams at lower latitudes (~1–22 g N/m2). We also demonstrated that NH4+ sorption to stream sediment may be an important physicochemical N storage mechanism that responds to short-term fluctuations in streamflow and governs the mobility of inorganic N. Altogether, this research illustrates the importance of quantifying N storage within stream corridors when evaluating the significance of internal cycling and physical retention processes that modulate N availability.

10ahyporheic zone10aMcMurdo LTER10anitrogen cycling10anutrient budget10aorganic matter10aperiphyton1 aSingley, Joel, G.1 aSalvatore, Mark, R.1 aGooseff, Michael, N.1 aMcKnight, Diane, M.1 aHinckley, Eve-Lyn, S. uhttps://www.journals.uchicago.edu/doi/10.1086/72567602567nas a2200337 4500008004100000245010300041210006900144260001200213300000900225490000700234520158600241653001601827653001201843653001401855653001901869653001801888100002401906700002201930700002401952700002001976700002101996700002102017700002502038700002102063700002102084700001902105700002102124700001702145700002102162856004602183 2023 eng d00aThe distribution of surface soil moisture over space and time in eastern Taylor Valley, Antarctica0 adistribution of surface soil moisture over space and time in eas c06/2023 a31700 v153 a

Available soil moisture is thought to be the limiting factor for most ecosystem processes in the cold polar desert of the McMurdo Dry Valleys (MDVs) of Antarctica. Previous studies have shown that microfauna throughout the MDVs are capable of biological activity when sufficient soil moisture is available (~2–10% gravimetric water content), but few studies have attempted to quantify the distribution, abundance, and frequency of soil moisture on scales beyond that of traditional field work or local field investigations. In this study, we present our work to quantify the soil moisture content of soils throughout the Fryxell basin using multispectral satellite remote sensing techniques. Our efforts demonstrate that ecologically relevant abundances of liquid water are common across the landscape throughout the austral summer. On average, the Fryxell basin of Taylor Valley is modeled as containing 1.5 ± 0.5% gravimetric water content (GWC) across its non-fluvial landscape with ~23% of the landscape experiencing an average GWC > 2% throughout the study period, which is the observed limit of soil nematode activity. These results indicate that liquid water in the soils of the MDVs may be more abundant than previously thought, and that the distribution and availability of liquid water is dependent on both soil properties and the distribution of water sources. These results can also help to identify ecological hotspots in the harsh polar Antarctic environment and serve as a baseline for detecting future changes in the soil hydrological regime.

10aDry valleys10aecology10ahydrology10aremote sensing10asoil moisture1 aSalvatore, Mark, R.1 aBarrett, John, E.1 aFackrell, Laura, E.1 aSokol, Eric, R.1 aLevy, Joseph, S.1 aKuentz, Lily, C.1 aGooseff, Michael, N.1 aAdams, Byron, J.1 aPower, Sarah, N.1 aKnightly, Paul1 aMatul, Haley, M.1 aSzutu, Brian1 aDoran, Peter, T. uhttps://www.mdpi.com/2072-4292/15/12/317002076nas a2200313 4500008004100000245008700041210006900128260001200197300001100209490000800220520114900228653002801377653002701405653002401432653002401456653001301480653000901493100001301502700002501515700001601540700002201556700001701578700001801595700001601613700002401629700002001653700001701673856007201690 2023 eng d00aEcological stoichiometry drives the evolution of soil nematode life history traits0 aEcological stoichiometry drives the evolution of soil nematode l c02/2023 a1088910 v1773 a

Ecological stoichiometry is a useful theoretical framework for understanding the sources and controls on nutrient availability that structure the composition and diversity of biotic communities. One such relationship is that organismal development rate is positively linked to cellular Phosphorus (P). We hypothesized that P availability, relative to other nutrients, e.g., nitrogen and carbon, would drive the evolution of traits associated with organismal growth and development. We examined the effects of P availability both in situ and in vitro, on free-living soil nematodes. We found that P-deficient environments produce predictable changes in the ecology and evolution of important life history traits. Our results identify altered rRNA gene copy number and subsequent changes in gene expression and protein synthesis as mechanisms by which P-deficiency influences these traits. These findings have important implications for explaining soil ecological and evolutionary patterns across multiple levels of organization, including the structure and functioning of organisms, populations, communities, and ecosystems.

10aelemental stoichiometry10agrowth rate hypothesis10alife history theory10amolecular evolution10anematoda10arRNA1 aXue, Xia1 aAdhikari, Bishwo, N.1 aBall, Becky1 aBarrett, John, E.1 aMiao, Jinxin1 aPerkes, Ammon1 aMartin, Mac1 aSimmons, Breana, L.1 aWall, Diana, H.1 aAdams, Byron uhttps://www.sciencedirect.com/science/article/pii/S003807172200348000724nas a2200229 4500008004100000022001400041245005500055210005100110260001200161300000800173490000700181653001200188653001200200653001600212653001600228653001200244100002100256700002200277700002700299700002000326856014800346 2023 eng d a0954-102000aExtreme cold (-69.1°C) in the McMurdo Dry Valleys0 aExtreme cold 691°C in the McMurdo Dry Valleys c03/2023 a1-40 v3010aclimate10aextreme10ameteorology10atemperature10aweather1 aDoran, Peter, T.1 aMyers, Krista, F.1 aMcKay, Christopher, P.1 aBromwich, David uhttps://www.cambridge.org/core/journals/antarctic-science/article/extreme-cold-691c-in-the-mcmurdo-dry-valleys/3516874750E5EF96365A26E8D49CA4EC02098nas a2200265 4500008004100000022001400041245010400055210006900159260001200228300001200240490000800252520131200260653001601572653001501588653001801603653002501621653002101646653001301667653002201680100001601702700001401718700001401732700002901746856005701775 2023 eng d a0166-859500aHigh salt-induced PSI-supercomplex is associated with high CEF and attenuation of state transitions0 aHigh saltinduced PSIsupercomplex is associated with high CEF and c09/2023 a65 - 840 v1573 a

While PSI-driven cyclic electron flow (CEF) and assembly of thylakoid supercomplexes have been described in model organisms like Chlamydomonas reinhardtii, open questions remain regarding their contributions to survival under long-term stress. The Antarctic halophyte, C. priscuii UWO241 (UWO241), possesses constitutive high CEF rates and a stable PSI-supercomplex as a consequence of adaptation to permanent low temperatures and high salinity. To understand whether CEF represents a broader acclimation strategy to short- and long-term stress, we compared high salt acclimation between the halotolerant UWO241, the salt-sensitive model, C. reinhardtii, and a moderately halotolerant Antarctic green alga, C. sp. ICE-MDV (ICE-MDV). CEF was activated under high salt and associated with increased non-photochemical quenching in all three Chlamydomonas species. Furthermore, high salt-acclimated cells of either strain formed a PSI-supercomplex, while state transition capacity was attenuated. How the CEF-associated PSI-supercomplex interferes with state transition response is not yet known. We present a model for interaction between PSI-supercomplex formation, state transitions, and the important role of CEF for survival during long-term exposure to high salt.

10aacclimation10aAntarctica10aChlamydomonas10aCyclic electron flow10aPSI-supercomplex10asalinity10astate transitions1 aKalra, Isha1 aWang, Xin1 aZhang, Ru1 aMorgan-Kiss, Rachael, M. uhttps://link.springer.com/10.1007/s11120-023-01032-y02591nas a2200229 4500008004100000245013200041210006900173260001200242300000600254490000600260520183600266100002102102700002402123700001802147700001602165700002202181700002402203700003302227700002402260700002202284856005502306 2023 eng d00aImpact of meltwater flow intensity on the spatiotemporal heterogeneity of microbial mats in the McMurdo Dry Valleys, Antarctica0 aImpact of meltwater flow intensity on the spatiotemporal heterog c01/2023 a30 v33 a

The meltwater streams of the McMurdo Dry Valleys are hot spots of biological diversity in the climate-sensitive polar desert landscape. Microbial mats, largely comprised of cyanobacteria, dominate the streams which flow for a brief window of time (~10 weeks) over the austral summer. These communities, critical to nutrient and carbon cycling, display previously uncharacterized patterns of rapid destabilization and recovery upon exposure to variable and physiologically detrimental conditions. Here, we characterize changes in biodiversity, transcriptional responses and activity of microbial mats in response to hydrological disturbance over spatiotemporal gradients. While diverse metabolic strategies persist between marginal mats and main channel mats, data collected from 4 time points during the austral summer revealed a homogenization of the mat communities during the mid-season peak meltwater flow, directly influencing the biogeochemical roles of this stream ecosystem. Gene expression pattern analyses identified strong functional sensitivities of nitrogen-fixing marginal mats to changes in hydrological activities. Stress response markers detailed the environmental challenges of each microhabitat and the molecular mechanisms underpinning survival in a polar desert ecosystem at the forefront of climate change. At mid and end points in the flow cycle, mobile genetic elements were upregulated across all mat types indicating high degrees of genome evolvability and transcriptional synchronies. Additionally, we identified novel antifreeze activity in the stream microbial mats indicating the presence of ice-binding proteins (IBPs). Cumulatively, these data provide a new view of active intra-stream diversity, biotic interactions and alterations in ecosystem function over a high-flow hydrological regime.

1 aZoumplis, Angela1 aKolody, Bethany, C.1 aKaul, Drishti1 aZheng, Hong1 aVenepally, Pratap1 aMcKnight, Diane, M.1 aTakacs-Vesbach, Cristina, D.1 aDeVries, Arthur, L.1 aAllen, Andrew, E. uhttps://www.nature.com/articles/s43705-022-00202-802814nas a2200373 4500008004100000022001400041245010900055210006900164260001200233520170200245653002001947653001001967653001101977653001201988653001102000653001102011100002502022700001902047700001902066700001702085700002102102700002302123700002402146700002502170700002402195700002602219700002102245700002202266700002202288700002102310700001802331700002202349856006902371 2023 eng d a0886-623600aLong-term changes in concentration and yield of riverine dissolved silicon from the poles to the tropics0 aLongterm changes in concentration and yield of riverine dissolve c08/20233 a

Riverine exports of silicon (Si) influence global carbon cycling through the growth of marine diatoms, which account for ∼25% of global primary production. Climate change will likely alter river Si exports in biome-specific ways due to interacting shifts in chemical weathering rates, hydrologic connectivity, and metabolic processes in aquatic and terrestrial systems. Nonetheless, factors driving long-term changes in Si exports remain unexplored at local, regional, and global scales. We evaluated how concentrations and yields of dissolved Si (DSi) changed over the last several decades of rapid climate warming using long-term datasets from 60 rivers and streams spanning the globe (e.g., Antarctic, tropical, temperate, boreal, alpine, Arctic systems). We show that widespread changes in river DSi concentration and yield have occurred, with the most substantial shifts occurring in alpine and polar regions. The magnitude and direction of trends varied within and among biomes, were most strongly associated with differences in land cover, and were often independent of changes in river discharge. These findings indicate that there are likely diverse mechanisms driving change in river Si biogeochemistry that span the land-water interface, which may include glacial melt, changes in terrestrial vegetation, and river productivity. Finally, trends were often stronger in months outside of the growing season, particularly in temperate and boreal systems, demonstrating a potentially important role of shifting seasonality for the flux of Si from rivers. Our results have implications for the timing and magnitude of silica processing in rivers and its delivery to global oceans.

10abiogeochemistry10ariver10asilica10asilicon10astream10atrends1 aJankowski, Kathi, Jo1 aJohnson, Keira1 aSethna, Lienne1 aJulian, Paul1 aWymore, Adam, S.1 aShogren, Arial, J.1 aThomas, Patrick, K.1 aSullivan, Pamela, L.1 aMcKnight, Diane, M.1 aMcDowell, William, H.1 aHeindel, Ruth, C1 aJones, Jeremy, B.1 aWollheim, Wilfred1 aAbbott, Benjamin1 aDeegan, Linda1 aCarey, Joanna, C. uhttps://agupubs.onlinelibrary.wiley.com/doi/10.1029/2022GB00767802541nas a2200241 4500008004100000245010600041210006900147260001200216520180200228653001202030653000602042653001802048653001902066653001302085653001902098653000602117653002202123100002202145700002202167700002102189700002402210856006502234 2023 eng d00aNitrogen fixation facilitates stream microbial mat biomass across the McMurdo Dry Valleys, Antarctica0 aNitrogen fixation facilitates stream microbial mat biomass acros c07/20233 a

Nitrogen (N) fixation is a fundamental mechanism by which N enters streams. Yet, because of modern N saturation, it is difficult to study the importance of N-fixation to stream nutrient budgets. Here, we utilized relatively simple and pristine McMurdo Dry Valley streams to investigate the role of N-fixing Nostoc abundance, streamwater dissolved inorganic N (DIN) concentration, and distance from the source glacier in regulating the elemental and isotopic composition of three microbial mat types (black, orange, and green) at the landscape scale. We found Nostoc-based black mats were the most enriched in δ15N, and δ15N signatures of mats increased where Nostoc was abundant, but did not surpass the atmospheric standard (δ15N ≈ 0‰). Furthermore, green and orange mat δ15N signatures became more depleted with increasing DIN, indicating that mats utilize glacial meltwater-sourced N when available. The distance from the source glacier explained limited variability in mat δ15N across sites, indicating the influence of individual stream characteristics on N spiraling. To further explore longitudinal N spiraling processes generating observed δ15Ν patterns, we developed a simple steady-state mathematical model. Analysis of plausible scenarios with this model confirmed that streams both have the capacity to remove allochthonous DIN over the plausible range of inputs, and that internal N sources are required to account for δ15N signatures and observed DIN concentrations at stream outlets. Collectively, these data and modeling results demonstrate that N-fixation exerts substantial influence within and across these streams, and is presumably dependent upon interconnected organic matter reserves, mineralization rates, and geomorphology.

10abiofilm10aC10acyanobacteria10ahyporheic zone10aMCM LTER10amineralization10aN10aP biogeochemistry1 aKohler, Tyler, J.1 aSingley, Joel, G.1 aWlostowski, Adam1 aMcKnight, Diane, M. uhttps://link.springer.com/article/10.1007/s10533-023-01069-001387nas a2200157 4500008004100000022001400041245006900055210006900124260001200193300001600205490000700221520088600228100002701114700002101141856006701162 2023 eng d a0024-359000aSediment oxygen consumption in Antarctic subglacial environments0 aSediment oxygen consumption in Antarctic subglacial environments c07/2023 a1557 - 15660 v683 a

Oxygen consumption in aquatic sediments is an indicator of overall biological activity of the ecosystem. As such, rates of sedimentary oxygen utilization are well documented for much of the open oceans and freshwater lakes. However, there are few direct measurements of sedimentary oxygen consumption from Antarctic subglacial aquatic sediments. We report the first microsensor oxygen profiles and derived sedimentary oxygen consumption rates from beneath the Ross Ice Shelf and a subglacial lake beneath the West Antarctic Ice Sheet. Rates of oxygen consumption in these two environments are relatively low, but comparable to those reported from ice-free polar oceans and oligotrophic Arctic lakes. Our study demonstrates the presence of oxygen within Antarctic subglacial aquatic sediments and its importance for oxygen-consuming microorganisms living in these ecosystems.

1 aMichaud, Alexander, B.1 aPriscu, John, C. uhttps://aslopubs.onlinelibrary.wiley.com/doi/10.1002/lno.1236602845nas a2200121 4500008004100000245008700041210006900128260005700197490000900254520237900263100002302642856005802665 2023 eng d00aSnow distribution and influence in Taylor Valley, Antarctica, using remote sensing0 aSnow distribution and influence in Taylor Valley Antarctica usin aBaton Rouge, LAbLouisiana State Universityc04/20230 vM.S.3 a

The McMurdo Dry Valleys is the largest ice-free area in Antarctica, but seasonal snow covers the valley floors sporadically throughout the year. In this study, a model to estimate areal snow coverage from satellite imagery was created. An area-volume model was created to estimate the amount of snow water equivalent (SWE) from the snow area extracted from the imagery. Snow cover influences the total albedo, the hydrologic budget, and the soil moisture and soil temperature in Taylor Valley (TV). Quantifying snow precipitation in TV is challenging because snow redistributes with winds, sublimates, or melts within a short period. Previous estimates found the amount of snow precipitation in TV is small, less than 100 mm/a. (SWE); even so, snow cover may influence processes in the valley. To better understand the controls and feedbacks of snow cover in the valley, a long-term record of spatially distributed abundance is required. This research creates a long-term record of snow cover data in TV using satellite images. The area of snowpacks was calculated by creating a classification scheme based on the brightness of panchromatic images. During the 2021-2022 field season, 250 m x 250 m sampling quadrats were surveyed to approximate how area and volume relate to SWE. Volumetric SWE was calculated by measuring in situ the length, width, depth, and density of each snowpack in the quadrat. There is a strong relationship between the area and the volume of the snowpacks (R2=0.942, P=0.182). With this information, estimates of the SWE can be made from the area calculated from satellite imagery. The average snow area for the entire extent of TV in late winter/early summer (September-December) from 2004 to 2022 is 65.26 km2, the average SWE is 0.0310 km3, and the average SWE depth is 75.72 mm. The amount of areal snow coverage is important when calculating the energy balance of TV, as well as understanding the availability of soil moisture to the soil ecosystem year-to-year. The available SWE can also influence seasonal surface and subsurface hydrology. While most precipitated snow in TV will sublimate or be redistributed by the wind, it is important to quantify how much snow has accumulated each season, especially with a warming climate, which could drastically influence snow accumulation and dynamics in TV.

1 aMcNulty, Katherine uhttps://digitalcommons.lsu.edu/gradschool_theses/574901137nas a2200193 4500008004100000245005600041210005300097260001200150300001100162490000700173520056500180100002100745700001800766700001400784700002300798700002900821700002600850856006700876 2022 eng d00aAn Antarctic alga that can survive the extreme cold0 aAntarctic alga that can survive the extreme cold c05/2022 a7408380 v103 a

Microscopic algae are tougher than you might think. Some can even survive the extreme cold. In this article, we describe one of the coolest algae of all, the Antarctic green alga called Chlamydomonas sp. UWO241. This one-celled super-organism lives deep in the frigid waters of a remote and permanently ice-covered lake in Antarctica. How does this little alga thrive in such a barren and unwelcoming place? Well, dive into this article to learn how studying the genome of UWO241 is helping scientists better understand this amazingly hardy alga.

1 aSmith, David, R.1 aLeung, Arthur1 aZhang, Xi1 aCvetkovska, Marina1 aMorgan-Kiss, Rachael, M.1 aHüner, Norman, P. A. uhttps://kids.frontiersin.org/articles/10.3389/frym.2022.74083802039nas a2200169 4500008004100000245013000041210007100171260001200242520142300254100002601677700001601703700001201719700002401731700002101755700002901776856006401805 2022 eng d00aAntarctic lake phytoplankton and bacteria from near‐surface waters exhibit high sensitivity to climate‐driven disturbance0 aAntarctic lake phytoplankton and bacteria from near‐surface wate c07/20223 a

The McMurdo Dry Valleys (MDVs), Antarctica, represent a cold, desert ecosystem poised on the threshold of melting and freezing water. The MDVs have experienced dramatic signs of climatic change, most notably a warm austral summer in 2001–2002 that caused widespread flooding, partial ice cover loss and lake level rise. To understand the impact of these climatic disturbances on lake microbial communities, we simulated lake level rise and ice-cover loss by transplanting dialysis-bagged communities from selected depths to other locations in the water column or to an open water perimeter moat. Bacteria and eukaryote communities residing in the surface waters (5 m) exhibited shifts in community composition when exposed to either disturbance, while microbial communities from below the surface were largely unaffected by the transplant. We also observed an accumulation of labile dissolved organic carbon in the transplanted surface communities. In addition, there were taxa-specific sensitivities: cryptophytes and Actinobacteria were highly sensitive particularly to the moat transplant, while chlorophytes and several bacterial taxa increased in relative abundance or were unaffected. Our results reveal that future climate-driven disturbances will likely undermine the stability and productivity of MDV lake phytoplankton and bacterial communities in the surface waters of this extreme environment.

1 aSherwell, Shasten, S.1 aKalra, Isha1 aLi, Wei1 aMcKnight, Diane, M.1 aPriscu, John, C.1 aMorgan-Kiss, Rachael, M. uhttps://onlinelibrary.wiley.com/doi/10.1111/1462-2920.1611302206nas a2200229 4500008004100000022001400041245015000055210006900205260001200274300001400286490000700300520141400307653001801721653001501739653001801754100002101772700001601793700001901809700002901828700002601857856009301883 2022 eng d a0954-102000aAntibiotic resistance genes and taxa analysis from mat and planktonic microbiomes of Antarctic perennial ice-covered Lake Fryxell and Lake Bonney0 aAntibiotic resistance genes and taxa analysis from mat and plank c12/2022 a408 - 4220 v343 a

The perennial ice-covered lakes of the Antarctic McMurdo Dry Valleys harbour oligotrophic microbial communities that are separated geographically from other aquatic systems. Their microbiomes include planktonic microbes as well as lift-off mat communities that emerge from the ice. We used the ShortBRED protein family profiler to quantify the antibiotic resistance genes (ARGs) from metagenomes of lift-off mats emerging from ice and from filtered water samples of Lake Fryxell and Lake Bonney. The overall proportion of ARG hits was similar to that found in temperate-zone rural ponds with moderate human inputs. Specific ARGs showed distinct distributions for the two lakes and for mat vs planktonic sources. Metagenomic taxa distributions showed that mat phototrophs consisted mainly of cyanobacteria or Betaproteobacteria, whereas the water column phototrophs were mainly protists. An enrichment culture of the Betaproteobacterium Rhodoferax antarcticus from a Lake Fryxell mat sample showed an unusual mat-forming phenotype not previously reported for this species. Its genome showed no ARGs associated with Betaproteobacteria but had ARGs consistent with a minor Pseudomonas component. The Antarctic lake mats and water showed specific ARGs distinctive to the mat and water sources, but overall ARG levels were similar to those of temperate water bodies with moderate human inputs.

10amicrobial mat10aRhodoferax10aTaylor Valley1 aTallada, Sheetal1 aHall, Grant1 aBarich, Daniel1 aMorgan-Kiss, Rachael, M.1 aSlonczewski, Joan, L. uhttps://www.cambridge.org/core/product/identifier/S0954102022000360/type/journal_article02771nas a2200325 4500008004100000022001400041245013500055210006900190260001200259300001000271490000700281520179400288653001302082653001302095653001002108653001302118653002002131653001702151653001702168653003102185100002602216700001902242700002002261700002102281700001802302700002202320700002102342700002402363856005802387 2022 eng d a0022-364600aBlowin’ in the wind: Dispersal, structure, and metacommunity dynamics of aeolian diatoms in the McMurdo Sound region, Antarctica0 aBlowin in the wind Dispersal structure and metacommunity dynamic c02/2022 a36-540 v583 a

Diatom metacommunities are structured by environmental, historical, and spatial factors that are often attributed to organism dispersal. In the McMurdo Sound region (MSR) of Antarctica, wind connects aquatic habitats through delivery of inorganic and organic matter. We evaluated the dispersal of diatoms in aeolian material and its relation to the regional diatom metacommunity using light microscopy and 18S rRNA high-throughput sequencing. The concentration of diatoms ranged from 0 to 8.76 * 106 valves · g-1 dry aeolian material. Up to 15% of whole cells contained visible protoplasm, indicating that up to 3.43 * 104 potentially viable individuals could be dispersed in a year to a single 2 cm2 site. Diatom DNA and RNA was detected at each site, reinforcing the likelihood that we observed dispersal of viable diatoms. Of the 50 known morphospecies in the MSR, 72% were identified from aeolian material using microscopy. Aeolian community composition varied primarily by site. Meanwhile, each aeolian community was comprised of morphospecies found in aquatic communities from the same lake basin. These results suggest that aeolian diatom dispersal in the MSR is spatially structured, is predominantly local, and connects local aquatic habitats via a shared species pool. Nonetheless, aeolian community structure was distinct from that of aquatic communities, indicating that intrahabitat dispersal and environmental filtering also underlie diatom metacommunity dynamics. The present study confirms that a large number of diatoms are passively dispersed by wind across a landscape characterized by aeolian processes, integrating the regional flora and contributing to metacommunity structure and landscape connectivity.

10a18S rRNA10aairborne10aalgae10aassembly10aBacillariophyta10abiogeography10aconnectivity10ahigh-throughput sequencing1 aSchulte, Nicholas, O.1 aKhan, Alia, L.1 aSmith, Emma, W.1 aZoumplis, Angela1 aKaul, Drishti1 aAllen, Andrew, E.1 aAdams, Byron, J.1 aMcKnight, Diane, M. uhttps://onlinelibrary.wiley.com/doi/10.1111/jpy.1322302454nas a2200277 4500008004100000245020000041210006900241260001200310300001400322490000800336520152000344653001501864653001401879653002501893653001801918653001701936653000801953100002401961700001601985700002102001700001902022700001802041700002302059700002902082856006502111 2022 eng d00aCyclic electron flow (CEF) and ascorbate pathway activity provide constitutive photoprotection for the photopsychrophile, Chlamydomonas sp. UWO 241 (renamed Chlamydomonas priscuii)0 aCyclic electron flow CEF and ascorbate pathway activity provide  c03/2022 a235 - 2500 v1513 a

Under environmental stress, plants and algae employ a variety of strategies to protect the photosynthetic apparatus and maintain photostasis. To date, most studies on stress acclimation have focused on model organisms which possess limited to no tolerance to stressful extremes. We studied the ability of the Antarctic alga Chlamydomonas sp. UWO 241 (UWO 241) to acclimate to low temperature, high salinity or high light. UWO 241 maintained robust growth and photosynthetic activity at levels of temperature (2 °C) and salinity (700 mM NaCl) which were nonpermissive for a mesophilic sister species, Chlamydomonas raudensis SAG 49.72 (SAG 49.72). Acclimation in the mesophile involved classic mechanisms, including downregulation of light harvesting and shifts in excitation energy between photosystem I and II. In contrast, UWO 241 exhibited high rates of PSI-driven cyclic electron flow (CEF) and a larger capacity for nonphotochemical quenching (NPQ). Furthermore, UWO 241 exhibited constitutively high activity of two key ascorbate cycle enzymes, ascorbate peroxidase and glutathione reductase and maintained a large ascorbate pool. These results matched the ability of the psychrophile to maintain low ROS under short-term photoinhibition conditions. We conclude that tight control over photostasis and ROS levels are essential for photosynthetic life to flourish in a native habitat of permanent photooxidative stress. We propose to rename this organism Chlamydomonas priscuii.

10aAntarctica10aascorbate10aCyclic electron flow10aPhotosystem I10aPsychrophile10aROS1 aStahl-Rommel, Sarah1 aKalra, Isha1 aD'Silva, Susanna1 aHahn, Mark, M.1 aPopson, Devon1 aCvetkovska, Marina1 aMorgan-Kiss, Rachael, M. uhttps://link.springer.com/article/10.1007/s11120-021-00877-502474nas a2200229 4500008004100000245007500041210006900116260001200185300001800197490000800215520178800223653001502011653001602026653002802042653000802070653002202078653000902100100002502109700002502134700002402159856006102183 2022 eng d00aDissolved organic carbon chemostasis in Antarctic polar desert streams0 aDissolved organic carbon chemostasis in Antarctic polar desert s c07/2022 ae2021JG0066490 v1273 a

Dissolved organic carbon (DOC) is a key variable impacting stream biogeochemical processes. The relationship between DOC concentration (C) and stream discharge (q) can elucidate spatial and temporal DOC source dynamics in watersheds. In the ephemeral glacial meltwater streams of the McMurdo Dry Valleys (MDV), Antarctica, the C-q relationship has been applied to dissolved inorganic nitrogen and weathering solutes including silica, which all exhibit chemostatic C-q behavior; but DOC-q dynamics have not been studied. DOC concentrations here are low compared to temperate streams, in the range of 0.1-2 mg C l-1, and their chemical signal clearly indicates derivation from microbial biomass (benthic mats and hyporheic biofilm). To investigate whether the DOC generation rate from these autochthonous organic matter pools was sufficient to maintain chemostasis for DOC, despite these streams' large diel and interannual fluctuations in discharge, we fit the long-term DOC-q data to a power law and an advection-reaction model. Model outputs and coefficients of variation characterize the DOC-q relationship as chemostatic for several MDV streams. We propose a conceptual model in which hyporheic carbon storage, hyporheic exchange rates, and net DOC generation rates are key interacting components that enable chemostatic DOC-q behavior in MDV streams. This model clarifies the role of autochthonous carbon stores in maintaining DOC chemostasis and may be useful for examining these relationships in temperate systems, which typically have larger sources of bioavailable autochthonous organic carbon than MDV streams but where this autochthonous signal could be masked by a stronger allochthonous contribution.

10aAntarctica10achemostasis10aconcentration-discharge10aDOC10aephemeral streams10aLTER1 aTorrens, Christa, L.1 aGooseff, Michael, N.1 aMcKnight, Diane, M. uhttps://onlinelibrary.wiley.com/doi/10.1029/2021JG00664902367nas a2200289 4500008004100000245011400041210006900155260001200224300001400236490000600250520147700256653001501733653002201748653002201770653001001792100002601802700002401828700002701852700002101879700001801900700001701918700002001935700001701955700001701972700002401989856006402013 2022 eng d00aElevational constraints on the composition and genomic attributes of microbial communities in Antarctic soils0 aElevational constraints on the composition and genomic attribute c01/2022 ae01330-210 v73 a

The inland soils found on the Antarctic continent represent one of the more challenging environments for microbial life on Earth. Nevertheless, Antarctic soils harbor unique bacterial and archaeal (prokaryotic) communities able to cope with extremely cold and dry conditions. These communities are not homogeneous, and the taxonomic composition and functional capabilities (genomic attributes) of these communities across environmental gradients remain largely undetermined. We analyzed the prokaryotic communities in soil samples collected from across the Shackleton Glacier region of Antarctica by coupling quantitative PCR, marker gene amplicon sequencing, and shotgun metagenomic sequencing. We found that elevation was the dominant factor explaining differences in the structures of the soil prokaryotic communities, with the drier and saltier soils found at higher elevations harboring less diverse communities and unique assemblages of cooccurring taxa. The higher-elevation soil communities also had lower maximum potential growth rates (as inferred from metagenome-based estimates of codon usage bias) and an overrepresentation of genes associated with trace gas metabolism. Together, these results highlight the utility of assessing community shifts across pronounced environmental gradients to improve our understanding of the microbial diversity found in Antarctic soils and the strategies used by soil microbes to persist at the limits of habitability.

10aAntarctica10amicrobial ecology10asoil microbiology10asoils1 aDragone, Nicholas, B.1 aHenley, Jessica, B.1 aHolland-Moritz, Hannah1 aDiaz, Melisa, A.1 aHogg, Ian, D.1 aLyons, Berry1 aWall, Diana, H.1 aAdams, Byron1 aFierer, Noah1 aMackelprang, Rachel uhttps://journals.asm.org/doi/full/10.1128/msystems.01330-2103274nas a2200313 4500008004100000245011700041210006900158260001200227300001100239490000700250520227300257653001502530653002502545653001802570653002702588653002802615653002302643653001902666100002202685700002802707700002302735700002302758700002502781700002202806700002202828700001502850700002302865856007202888 2022 eng d00aLipid biomarkers from microbial mats on the McMurdo Ice Shelf, Antarctica: Signatures for life in the cryosphere0 aLipid biomarkers from microbial mats on the McMurdo Ice Shelf An c06/2022 a9036210 v133 a

Persistent cold temperatures, a paucity of nutrients, freeze-thaw cycles, and the strongly seasonal light regime make Antarctica one of Earth’s least hospitable surface environments for complex life. Cyanobacteria, however, are well-adapted to such conditions and are often the dominant primary producers in Antarctic inland water environments. In particular, the network of meltwater ponds on the ‘dirty ice’ of the McMurdo Ice Shelf is an ecosystem with extensive cyanobacteria-dominated microbial mat accumulations. This study investigated intact polar lipids (IPLs), heterocyte glycolipids (HGs), and bacteriohopanepolyols (BHPs) in combination with 16S and 18S rRNA gene diversity in microbial mats of twelve ponds in this unique polar ecosystem. To constrain the effects of nutrient availability, temperature and freeze-thaw cycles on the lipid membrane composition, lipids were compared to stromatolite-forming cyanobacterial mats from ice-covered lakes in the McMurdo Dry Valleys as well as from (sub)tropical regions and hot springs. The 16S rRNA gene compositions of the McMurdo Ice Shelf mats confirm the dominance of Cyanobacteria and Proteobacteria while the 18S rRNA gene composition indicates the presence of Ochrophyta, Chlorophyta, Ciliophora, and other microfauna. IPL analyses revealed a predominantly bacterial community in the meltwater ponds, with archaeal lipids being barely detectable. IPLs are dominated by glycolipids and phospholipids, followed by aminolipids. The high abundance of sugar-bound lipids accords with a predominance of cyanobacterial primary producers. The phosphate-limited samples from the (sub)tropical, hot spring, and Lake Vanda sites revealed a higher abundance of aminolipids compared to those of the nitrogen-limited meltwater ponds, affirming the direct affects that N and P availability have on IPL compositions. The high abundance of polyunsaturated IPLs in the Antarctic microbial mats suggests that these lipids provide an important mechanism to maintain membrane fluidity in cold environments. High abundances of HG keto-ols and HG keto-diols, produced by heterocytous cyanobacteria, further support these findings and reveal a unique distribution compared to those from warmer climates.

10aAntarctica10abacteriohopanepolyol10acyanobacteria10aheterocyte glycolipids10ahomeoviscous adaptation10aintact polar lipid10amicrobial mats1 aEvans, Thomas, W.1 aKalambokidis, Maria, J.1 aJungblut, Anne, D.1 aMillar, Jasmin, L.1 aBauersachs, Thorsten1 aGrotheer, Hendrik1 aMackey, Tyler, J.1 aHawes, Ian1 aSummons, Roger, E. uhttps://www.frontiersin.org/articles/10.3389/fmicb.2022.903621/full01993nas a2200253 4500008004100000022001400041245010200055210006900157260001200226300001100238490000700249520122000256653001501476653000901491653002401500653002201524653001701546653002301563100002501586700002401611700002101635700002101656856006201677 2022 eng d a0885-608700aLong-term stream hydrology and meteorology of a polar desert, the McMurdo Dry Valleys, Antarctica0 aLongterm stream hydrology and meteorology of a polar desert the  c06/2022 ae146230 v363 a

The McMurdo Dry Valleys (MDVs; 77.50°S, 162.25°E) make up the largest ice-free region of Antarctica at 3500 km2. Their position near the coast of the Ross Sea provides for a milder climate than much of the rest of the continent. Alpine and piedmont glaciers in the MDVs melt during the austral summer providing water to down gradient streams and terminal lakes on valley floors. There are currently 14 meteorological stations and 17 stream gauges operating across the MDVs, some with continuous records that go back to 1969. This relatively high density of monitoring stations reflects the fact that glaciers of different sizes and elevation ranges are the main source of water to streams. Thus, each glacier represents a different watershed. The bulk of these records start in the late 1980s/early 1990s. These data collection activities directly support research endeavors of the McMurdo Dry Valleys Long Term Ecological Research project, as well as a host of other science groups working in the MDVs. As such, both real time data and archived data from these sites is available through the online database interface of the project (https://mcmlter.org).

10aAntarctica10aLTER10aMcMurdo Dry Valleys10ameltwater streams10apolar desert10aresearch catchment1 aGooseff, Michael, N.1 aMcKnight, Diane, M.1 aDoran, Peter, T.1 aFountain, Andrew uhttps://onlinelibrary.wiley.com/doi/abs/10.1002/hyp.1462303572nas a2200301 4500008004100000245010000041210006900141260001200210300001100222490000800233520264300241653002302884653001002907653002002917653002202937653002602959653001902985653001603004100002603020700002103046700002303067700001403090700002603104700002303130700001603153700002903169856007203198 2022 eng d00aPhotosynthetic adaptation to polar life: Energy balance, photoprotection and genetic redundancy0 aPhotosynthetic adaptation to polar life Energy balance photoprot c01/2022 a1535570 v2683 a

The persistent low temperature that characterize polar habitats combined with the requirement for light for all photoautotrophs creates a conundrum. The absorption of too much light at low temperature can cause an energy imbalance that decreases photosynthetic performance that has a negative impact on growth and can affect long-term survival. The goal of this review is to survey the mechanism(s) by which polar photoautotrophs maintain cellular energy balance, that is, photostasis to overcome the potential for cellular energy imbalance in their low temperature environments. Photopsychrophiles are photosynthetic organisms that are obligately adapted to low temperature (0-15 °C) but usually die at higher temperatures (≥20 °C). In contrast, photopsychrotolerant species can usually tolerate and survive a broad range of temperatures (5-40 °C). First, we summarize the basic concepts of excess excitation energy, energy balance, photoprotection and photostasis and their importance to survival in polar habitats. Second, we compare the photoprotective mechanisms that underlie photostasis and survival in aquatic cyanobacteria and green algae as well as terrestrial Antarctic and Arctic plants. We show that polar photopsychrophilic and photopsychrotolerant organisms attain energy balance at low temperature either through a regulated reduction in the efficiency of light absorption or through enhanced capacity to consume photosynthetic electrons by the induction of O2 as an alternative electron acceptor. Finally, we compare the published genomes of three photopsychrophilic and one photopsychrotolerant alga with five mesophilic green algae including the model green alga, Chlamydomonas reinhardtii. We relate our genomic analyses to photoprotective mechanisms that contribute to the potential attainment of photostasis. Finally, we discuss how the observed genomic redundancy in photopsychrophilic genomes may confer energy balance, photoprotection and resilience to their harsh polar environment. Primary production in aquatic, Antarctic and Arctic environments is dependent on diverse algal and cyanobacterial communities. Although mosses and lichens dominate the Antarctic terrestrial landscape, only two extant angiosperms exist in the Antarctic. The identification of a single ‘molecular key’ to unravel adaptation of photopsychrophily and photopsychrotolerance remains elusive. Since these photoautotrophs represent excellent biomarkers to assess the impact of global warming on polar ecosystems, increased study of these polar photoautotrophs remains essential.

10agenomic redundancy10alight10aphotoprotection10aphotopsychrophily10aphotopsychrotolerance10aPhotosynthesis10atemperature1 aHüner, Norman, P. A.1 aSmith, David, R.1 aCvetkovska, Marina1 aZhang, Xi1 aIvanov, Alexander, G.1 aSzyszka-Mroz, Beth1 aKalra, Isha1 aMorgan-Kiss, Rachael, M. uhttps://www.sciencedirect.com/science/article/pii/S017616172100196602569nas a2200373 4500008004100000245008100041210006900122260004300191520135400234100001701588700001801605700002201623700002101645700002001666700003001686700002101716700002401737700002301761700001801784700002101802700002301823700002201846700002001868700002301888700002401911700001601935700002301951700002401974700002101998700001902019700001802038700002002056856011902076 2022 eng d00aTen scientific messages on risks and opportunities for life in the Antarctic0 aTen scientific messages on risks and opportunities for life in t bAntarctic Environments Portalc02/20223 a

Initiated by the SCAR scientific research programme “Antarctic Thresholds – Ecosystem Resilience and Adaptation” (AnT-ERA, 2013-2021), 26 experts synthesized knowledge on impacts and risks of climate-change on biological processes and ecosystem functions in the Antarctic. The ten main scientific messages that emerged addressed (1) accelerating marine and terrestrial biogeochemical cycles, (2) response to ocean acidification, (3) ecological changes in climate change hot spots, (4) unexpected dynamism of marine seafloor communities, (5) biodiversity shifts, (6) low temperature limitation of protein synthesis, (7) life intrinsically linked to changing sea ice conditions, (8) pollution, (9) genetically distinct terrestrial populations under threat, and (10) newly discovered habitats. Two-thirds of the literature included in this synthesis was published between 2010 and 2020 and only one-third was published earlier. The fast mounting, recent decadal evidence indicates various Antarctic biological communities now experience climate stress, or will experience such stress in the coming decades. The responses of organisms, ecosystem functions and services to environmental changes are complex and varied. Key knowledge gaps remain and need addressing to adequately assess future prospects for life in the Antarctic.

1 aGutt, Julian1 aIsla, Enrique1 aXavier, José, C.1 aAdams, Byron, J.1 aAhn, In‐Young1 aCheng, C.‐H., Christina1 aColesie, Claudia1 aCummings, Vonda, J.1 aGriffiths, Huw, J.1 aHogg, Ian, D.1 aMcIntyre, Trevor1 aMeiners, Klaus, M.1 aPearce, David, A.1 aPeck, Lloyd, S.1 aPiepenburg, Dieter1 aReisinger, Ryan, R.1 aSaba, Grace1 aSchloss, Irene, R.1 aSignori, Camila, N.1 aSmith, Craig, R.1 aVacchi, Marino1 aVerde, Cinzia1 aWall, Diana, H. uhttps://environments.aq/publications/ten-scientific-messages-on-risks-and-opportunities-for-life-in-the-antarctic/02575nas a2200397 4500008004100000022001400041245006100055210005700116260001200173490000800185520139000193100002701583700002801610700001701638700002401655700003001679700001901709700002201728700002101750700002301771700002201794700002501816700002001841700002201861700002101883700002201904700003101926700002201957700002301979700002202002700002402024700002102048700002202069700003302091856005302124 2022 eng d a0027-842400aThe time is right for an Antarctic biorepository network0 atime is right for an Antarctic biorepository network c12/20220 v1193 a

Antarctica is a central driver of the Earth’s climate and health. The Southern Ocean surrounding Antarctica serves as a major sink for anthropogenic CO2 and heat, and the loss of Antarctic ice sheets contributes significantly to sea level rise and will continue to do so as the loss of ice sheets accelerates, with sufficient water stores to raise sea levels by 58 m. Antarctica's marine environment is home to a number of iconic species, and the terrestrial realm harbors a remarkable oasis for life, much of which has yet to be discovered. Distinctive oceanographic features of the Southern Ocean—including the Antarctic Circumpolar Current, the Antarctic Polar Front, and exceptional depths surrounding the continent—coupled with chronically cold temperatures have fostered the evolution of a vast number of uniquely coldadapted species, many of which are found nowhere else on the Earth. The Antarctic marine biota, for example, displays the highest level of species endemism on the Earth. However, warming, ocean acidification, pollution, and commercial exploitation threaten the integrity of Antarctic ecosystems. Understanding changes in the biota and its capacities for adaptation is imperative for establishing effective policies for mitigating the impacts of climate change and sustaining the Antarctic ecosystems that are vital to global health.

1 aO’Brien, Kristin, M.1 aCrockett, Elizabeth, L.1 aAdams, Byron1 aAmsler, Charles, D.1 aAppiah-Madson, Hannah, J.1 aCollins, Allen1 aDesvignes, Thomas1 aDetrich, William1 aDistel, Daniel, L.1 aEppley, Sarah, M.1 aFrable, Benjamin, W.1 aFranz, Nico, M.1 aGrim, Jeffrey, M.1 aKocot, Kevin, M.1 aMahon, Andrew, R.1 aMayfield-Meyer, Teresa, J.1 aMikucki, Jill, A.1 aMoser, William, E.1 aSchmull, Michaela1 aSeid, Charlotte, A.1 aSmith, Craig, R.1 aTodgham, Anne, E.1 aWatkins-Colwell, Gregory, J. uhttps://www.pnas.org/doi/10.1073/pnas.221280011901961nas a2200217 4500008004100000245010100041210006900142260001200211300001200223490000700235520124400242653003001486653001101516653002401527653001501551653002101566100002001587700002101607700002201628856009301650 2022 eng d00aValley-floor snowfall in Taylor Valley, Antarctica, from 1995 to 2017: Spring, summer and autumn0 aValleyfloor snowfall in Taylor Valley Antarctica from 1995 to 20 c08/2022 a325-3350 v343 a

We present an analysis of the 20 year snowfall dataset in Taylor Valley and the results of a new snow cover monitoring study. Snowfall has been measured at four sites in Taylor Valley from 1995 to 2017. We focus on valley-floor snowfall when wind does not exceed 5 m s-1, and we exclude winter from our analysis due to poor data quality. Snowfall averaged 11 mm water equivalent (w.e.) from 1995 to 2017 across all stations and ranged from 1 to 58 mm w.e. Standard deviations ranged from 3 to 17 mm w.e., highlighting the strong interannual variability of snowfall in Taylor Valley. During spring and autumn there is a spatial gradient in snowfall such that the coast received twice as much snowfall as more central and inland stations. We identified a changepoint in 2007 from increasing snowfall (3 mm w.e. yr-1) to decreasing snowfall (1 mm w.e. yr-1), which coincides with a shift from decreasing temperature to no detectable temperature trend. Daily camera imagery from 2007 to 2017 augments the snowfall measurements. The camera imagery revealed a near tripling of the average number of days with snow cover from 37 days between 2006 and 2012 to 106 days with snow cover between 2012 and 2017.

10aautomated weather station10acamera10aMcMurdo Dry Valleys10asnow cover10asnow persistence1 aMyers, Madeline1 aDoran, Peter, T.1 aMyers, Krista, F. uhttps://www.cambridge.org/core/product/identifier/S0954102022000256/type/journal_article03115nas a2200517 4500008004100000245008300041210006900124260001200193520161800205653001501823653002101838653002601859653001901885653001301904653001701917653002401934653002301958653001701981653001201998100001702010700001802027700002202045700002102067700002002088700003002108700002102138700002402159700002102183700002302204700001502227700001802242700002102260700002302281700002202304700002002326700002302346700002402369700001602393700002302409700002402432700002102456700001902477700001802496700002002514856006302534 2021 eng d00aAntarctic ecosystems in transition – life between stresses and opportunities0 aAntarctic ecosystems in transition life between stresses and opp c06/20213 a

Important findings from the second decade of the 21st century on the impact of environmental change on biological processes in the Antarctic were synthesised by 26 international experts. Ten key messages emerged that have stakeholder‐relevance and/or a high impact for the scientific community. They address (i) altered biogeochemical cycles, (ii) ocean acidification, (iii) climate change hotspots, (iv) unexpected dynamism in seabed‐dwelling populations, (v) spatial range shifts, (vi) adaptation and thermal resilience, (vii) sea ice related biological fluctuations, (viii) pollution, (ix) endangered terrestrial endemism and (x) the discovery of unknown habitats. Most Antarctic biotas are exposed to multiple stresses and considered vulnerable to environmental change due to narrow tolerance ranges, rapid change, projected circumpolar impacts, low potential for timely genetic adaptation, and migration barriers. Important ecosystem functions, such as primary production and energy transfer between trophic levels, have already changed, and biodiversity patterns have shifted. A confidence assessment of the degree of ‘scientific understanding’ revealed an intermediate level for most of the more detailed sub‐messages, indicating that process‐oriented research has been successful in the past decade. Additional efforts are necessary, however, to achieve the level of robustness in scientific knowledge that is required to inform protection measures of the unique Antarctic terrestrial and marine ecosystems, and their contributions to global biodiversity and ecosystem services.

10aadaptation10abenthic dynamism10abiogeochemical cycles10aclimate change10ainvasion10anew habitats10aocean acidification10aPrimary production10arange shifts10asea ice1 aGutt, Julian1 aIsla, Enrique1 aXavier, José, C.1 aAdams, Byron, J.1 aAhn, In‐Young1 aCheng, C.‐H., Christina1 aColesie, Claudia1 aCummings, Vonda, J.1 adi Prisco, Guido1 aGriffiths, Huw, J.1 aHawes, Ian1 aHogg, Ian, D.1 aMcIntyre, Trevor1 aMeiners, Klaus, M.1 aPearce, David, A.1 aPeck, Lloyd, S.1 aPiepenburg, Dieter1 aReisinger, Ryan, R.1 aSaba, Grace1 aSchloss, Irene, R.1 aSignori, Camila, N.1 aSmith, Craig, R.1 aVacchi, Marino1 aVerde, Cinzia1 aWall, Diana, H. uhttps://onlinelibrary.wiley.com/doi/full/10.1111/brv.1267900482nas a2200133 4500008004100000022001400041245011100055210006900166260001200235300001400247490000700261100001500268856006500283 2021 eng d a0006-356800aAntarctica during the Pandemic: Scaled-back field season prioritizes infrastructure, precious climate data0 aAntarctica during the Pandemic Scaledback field season prioritiz c05/2021 a434 - 4400 v711 aMayer, Amy uhttps://academic.oup.com/bioscience/article/71/5/434/618880004166nas a2200565 4500008004100000022001400041245007900055210006900134260001200203300001100215490000700226520245300233653001802686653002702704653001902731653001902750653001202769653001202781653001502793653008902808653002002897100002302917700002502940700002602965700002702991700002103018700002503039700001703064700002203081700002903103700002603132700002403158700002603182700002403208700002103232700001603253700002703269700002403296700002303320700002203343700002403365700002303389700001503412700002203427700002103449700002203470700002603492700002403518856005803542 2021 eng d a2150-892500aConnectivity: Insights from the U.S. Long Term Ecological Research Network0 aConnectivity Insights from the US Long Term Ecological Research  c05/2021 ae034320 v123 a

Ecosystems across the United States are changing in complex and surprising ways. Ongoing demand for critical ecosystem services requires an understanding of the populations and communities in these ecosystems in the future. This paper represents a synthesis effort of the U.S. National Science Foundation-funded Long-Term Ecological Research (LTER) network addressing the core research area of “populations and communities.” The objective of this effort was to show the importance of long-term data collection and experiments for addressing the hardest questions in scientific ecology that have significant implications for environmental policy and management. Each LTER site developed at least one compelling case study about what their site could look like in 50–100 yr as human and environmental drivers influencing specific ecosystems change. As the case studies were prepared, five themes emerged, and the studies were grouped into papers in this LTER Futures Special Feature addressing state change, connectivity, resilience, time lags, and cascading effects. This paper addresses the “connectivity” theme and has examples from the Phoenix (urban), Niwot Ridge (alpine tundra), McMurdo Dry Valleys (polar desert), Plum Island (coastal), Santa Barbara Coastal (coastal), and Jornada (arid grassland and shrubland) sites. Connectivity has multiple dimensions, ranging from multi-scalar interactions in space to complex interactions over time that govern the transport of materials and the distribution and movement of organisms. The case studies presented here range widely, showing how land-use legacies interact with climate to alter the structure and function of arid ecosystems and flows of resources and organisms in Antarctic polar desert, alpine, urban, and coastal marine ecosystems. Long-term ecological research demonstrates that connectivity can, in some circumstances, sustain valuable ecosystem functions, such as the persistence of foundation species and their associated biodiversity or, it can be an agent of state change, as when it increases wind and water erosion. Increased connectivity due to warming can also lead to species range expansions or contractions and the introduction of undesirable species. Continued long-term studies are essential for addressing the complexities of connectivity. The diversity of ecosystems within the LTER network is a strong platform for these studies.

10aalpine tundra10aAntarctic polar desert10aarid grassland10aarid shrubland10acoastal10aestuary10asalt marsh10aSpecial Feature: Forecasting Earth’s Ecosystems with Long-Term Ecological Research10aurban ecosystem1 aIwaniec, David, M.1 aGooseff, Michael, N.1 aSuding, Katharine, N.1 aJohnson, David, Samuel1 aReed, Daniel, C.1 aPeters, Debra, P. C.1 aAdams, Byron1 aBarrett, John, E.1 aBestelmeyer, Brandon, T.1 aCastorani, Max, C. N.1 aCook, Elizabeth, M.1 aDavidson, Melissa, J.1 aGroffman, Peter, M.1 aHanan, Niall, P.1 aHuenneke, L1 aJohnson, Pieter, T. J.1 aMcKnight, Diane, M.1 aMiller, Robert, J.1 aOkin, Gregory, S.1 aPreston, Daniel, L.1 aRassweiler, Andrew1 aRay, Chris1 aSala, Osvaldo, E.1 aSchooley, Robert1 aSeastedt, Timothy1 aSpasojevic, Marko, J.1 aVivoni, Enrique, R. uhttps://onlinelibrary.wiley.com/doi/10.1002/ecs2.343201995nas a2200205 4500008004100000245012000041210006900161260004800230490000900278520132200287653001501609653001101624653001801635653001201653653001501665653001301680100002401693700002401717856004801741 2021 eng d00aControls on microbial mat coverage and diatom species turnover in Antarctic desert streams: A transplant experiment0 aControls on microbial mat coverage and diatom species turnover i aBoulder, CObUniversity of Colorado Boulder0 vM.S.3 a

This thesis evaluates how polar desert streams regulate benthic microbial mat coverage, chlorophyll-a biomass, and diatom species composition. Microbial mats growing on rocks (eplithon) and on sandy substrate (epipelon) were reciprocally transplanted among four glaciers meltwater streams and monitored through time. The selected study streams were Green Creek, Bowles Creek, Delta Stream, Von Guerard Stream in the Lake Fryxell Basin in the McMurdo Dry Valleys, Antarctica. These streams vary in length, streamflow intermittency, and diatom community composition of microbial mats. Results demonstrate that streams strongly control mat biomass (coverage and chlorophyll-a) differently for epilithon and epipelon. However, diatom species composition did not vary between these growth habitats but instead varied by stream, suggesting adaptive niche separation related to environmental conditions. Diatom species composition of transplants in Green Creek became dissimilar from their initial stream communities suggesting downstream dispersal and within stream connectivity regulates community assembly. This experiment confirms that environmental characteristics and intra-stream dispersal processes exert strong control over maintaining microbial mat coverage, biomass accrual, and diatom species composition.

10aAntarctica10adiatom10amicrobial mat10astreams10atransplant10aturnover1 aDarling, Joshua, P.1 aMcKnight, Diane, M. uhttps://www.proquest.com/docview/263459098202809nas a2200337 4500008004100000245012000041210006900161260001200230300001800242490000800260520168500268653002201953653002001975653004901995653001902044653001202063653004302075653001902118653002402137653002102161653003102182100002102213700002402234700002202258700002402280700002402304700002502328700002402353700002502377856006902402 2021 eng d00aDiatoms in hyporheic sediments trace organic matter retention and processing in the McMurdo Dry Valleys, Antarctica0 aDiatoms in hyporheic sediments trace organic matter retention an c02/2021 ae2020JG0060970 v1263 a

In low‐nutrient streams in cold and arid ecosystems, the spiraling of autochthonous particulate organic matter (POM) may provide important nutrient subsidies downstream. Because of its lability and the spatial heterogeneity of processing in hyporheic sediments, the downstream transport and fate of autochthonous POM can be difficult to trace. In Antarctic McMurdo Dry Valley (MDV) streams, any POM retained in the hyporheic zone is expected to be derived from surface microbial mats that contain diatoms with long‐lasting silica frustules. We tested whether diatom frustules can be used to trace the retention of autochthonous POM in the hyporheic zone and whether certain geomorphic locations promote this process. The accumulation of diatom frustules in hyporheic sediments, measured as biogenic silica, was correlated with loss‐on‐ignition organic matter and sorbed ammonium, suggesting that diatoms can be used to identify locations where POM has been retained and processed over long timescales, regardless of whether the POM remains intact. In addition, by modeling the upstream sources of hyporheic diatom assemblages, we found that POM was predominantly derived from N‐fixing microbial mats of the genus Nostoc. In terms of spatial variability, we conclude that the hyporheic sediments adjacent to the stream channel that are regularly inundated by daily flood pulses are where the most POM has been retained over long timescales. Autochthonous POM is retained in hyporheic zones of low‐nutrient streams beyond the MDVs, and we suggest that biogenic silica and diatom composition can be used to identify locations where this transfer is most prevalent.

10abenthic processes10abiogenic silica10abiogeochemical cycles processes and modeling10acarbon cycling10adiatoms10agroundwater/surface water interactions10ahyporheic zone10aMcMurdo Dry Valleys10anitrogen cycling10aparticulate organic matter1 aHeindel, Ruth, C1 aDarling, Joshua, P.1 aSingley, Joel, G.1 aBergstrom, Anna, J.1 aMcKnight, Diane, M.1 aLukkari, Braeden, M.1 aWelch, Kathleen, A.1 aGooseff, Michael, N. uhttps://agupubs.onlinelibrary.wiley.com/doi/10.1029/2020JG00609701659nas a2200193 4500008004100000022001300041245008600054210006900140260001200209300001100221490000700232520104000239100001401279700002301293700002901316700002601345700002201371856007201393 2021 eng d a2589004200aDraft genome sequence of the Antarctic green alga Chlamydomonas sp. UWO2410 aDraft genome sequence of the Antarctic green alga IChlamydomonas c2021-02 a1020840 v243 a

Antarctica is home to an assortment of psychrophilic algae, which have evolved various survival strategies for coping with their frigid environments. Here, we explore Antarctic psychrophily by examining the ∼212 Mb draft nuclear genome of the green alga Chlamydomonas sp. UWO241, which resides within the water column of a perennially ice-covered, hypersaline lake. Like certain other Antarctic algae, UWO241 encodes a large number (≥37) of ice-binding proteins, putatively originating from horizontal gene transfer. Even more striking, UWO241 harbors hundreds of highly similar duplicated genes involved in diverse cellular processes, some of which we argue are aiding its survival in the Antarctic via gene dosage. Gene and partial gene duplication appear to be an ongoing phenomenon within UWO241, one which might be mediated by retrotransposons. Ultimately, we consider how such a process could be associated with adaptation to extreme environments but explore potential non-adaptive hypotheses as well.

1 aZhang, Xi1 aCvetkovska, Marina1 aMorgan-Kiss, Rachael, M.1 aHüner, Norman, P. A.1 aSmith, David, Roy uhttps://www.sciencedirect.com/science/article/pii/S258900422100052301860nas a2200205 4500008004100000022001400041245015300055210006900208260001200277520112000289100002201409700002001431700002001451700002501471700001701496700002401513700002501537700002401562856006801586 2021 eng d a2378-224200aFrom the Heroic Age to today: What diatoms from Shackleton's Nimrod expedition can tell us about the ecological trajectory of Antarctic ponds0 aFrom the Heroic Age to today What diatoms from Shackletons iNimr c07/20213 a

Biological invasion and environmental change pose major threats to ecosystems. While long-term ecological change is commonly evaluated through sediment cores in lakes, it is generally not feasible for smaller ponds, and spatial resolution is limited. Here, we analyze pond diatom communities collected during Shackleton's Nimrod expedition at Cape Royds, Antarctica, to compare with the same waterbodies a century later. We find historical samples to be almost identical to modern counterparts, and provide no evidence of exotic introductions despite increasing human activity. However, a shift occurred in the pond nearest Shackleton's hut, Pony Lake, which was dominated by Luticola muticopsis a century ago, and was replaced by Craspedostauros laevissimus. Both are endemic species previously and currently present at Cape Royds, and we hypothesize that a shift in conductivity accompanying changing precipitation patterns may be responsible. Collectively, these results provide important data for assessing human and climate impacts among Antarctic lacustrine habitats.

1 aKohler, Tyler, J.1 aHowkins, Adrian1 aSokol, Eric, R.1 aKopalová, Kateřina1 aCox, Aneliya1 aDarling, Joshua, P.1 aGooseff, Michael, N.1 aMcKnight, Diane, M. uhttps://aslopubs.onlinelibrary.wiley.com/doi/10.1002/lol2.1020002603nas a2200181 4500008004100000245007200041210006900113260001200182300001200194490000800206520196600214100002402180700002402204700001702228700002402245700002402269856012802293 2021 eng d00aGeochemistry of contrasting stream types, Taylor Valley, Antarctica0 aGeochemistry of contrasting stream types Taylor Valley Antarctic c01/2021 a425-4480 v1333 a

The McMurdo Dry Valley region is the largest ice-free area of Antarctica. Ephemeral streams flow here during the austral summer, transporting glacial meltwater to perennially ice-covered, closed basin lakes. The chemistry of 24 Taylor Valley streams was examined over the two-decade period of monitoring from 1993 to 2014, and the geochemical behavior of two streams of contrasting physical and biological character was monitored across the seven weeks of the 2010–2011 flow season. Four species dominate stream solute budgets: HCO3, Ca2+, Na+, and Cl, with SO42–, Mg2+, and K+ present in significantly lesser proportions. All streams contain dissolved silica at low concentrations. Across Taylor Valley, streams are characterized by their consistent anionic geochemical fingerprint of HCO3 > Cl > SO4, but there is a split in cation composition between 14 streams with Ca > Na > Mg > K and 10 streams with Na > Ca > Mg > K.


Andersen Creek is a first-order proglacial stream representative of the 13 short streams that flow <1.5 km from source to gage. Von Guerard is representative of 11 long streams 2–7 km in length characterized by extensive hyporheic zones. Both streams exhibit a strong daily cycle for solute load, temperature, dissolved oxygen, and pH, which vary in proportion to discharge. A well-expressed diurnal co-variation of pH with dissolved oxygen is observed for both streams that reflects different types of biological control. The relative consistency of Von Guerard composition over the summer flow season reflects chemostatic regulation, where water in transient storage introduced during times of high streamflow has an extended opportunity for water-sediment interaction, silicate mineral dissolution, and pore-water exchange.

1 aHarmon, Russell, S.1 aLeslie, Deborah, L.1 aLyons, Berry1 aWelch, Kathleen, A.1 aMcKnight, Diane, M. uhttps://pubs.geoscienceworld.org/gsa/gsabulletin/article/133/1-2/425/587799/Geochemistry-of-contrasting-stream-types-Taylor02981nas a2200253 4500008004100000022001400041245011200055210006900167260001200236300001400248490000700262520216600269653002102435653002602456653002602482100002802508700002202536700002202558700002102580700002202601700001802623700001502641856007102656 2021 eng d a1523-043000aThe magnitude and climate sensitivity of isotopic fractionation from ablation of Antarctic Dry Valley lakes0 amagnitude and climate sensitivity of isotopic fractionation from c12/2021 a352 - 3710 v533 a

There has been extensive research on the effects of evaporation on the isotopic ratio of lacustrine and marine water bodies; however, there are limited data on how ablation or sublimation from lake or sea ice influences the isotopic ratio of the residual water body. This is a challenging problem because there remains uncertainty on the magnitude of fractionation during sublimation and because ablation can involve mixed-phase processes associated with simultaneous sublimation, melting, evaporation, and refreezing. This uncertainty limits the ability to draw quantitative inferences on changing hydrological budgets from stable isotope records in arctic, Antarctic, and alpine lakes. Here, we use in situ measurements of the isotopic ratio of water vapor along with the gradient diffusion method to constrain the isotopic ratio of the ablating ice from two lakes in the McMurdo Dry Valleys, Antarctica. We find that during austral summer, the isotopic fractionation of ablation was insignificant during periods of boundary layer instability that are typical during midday when latent heat is highest. This implies that the loss of mass during these periods did not yield any isotopic enrichment to the residual lake mass. However, fractionation increased after midday when the boundary layer stabilized and the latent heat flux was small. This diurnal pattern was mirrored on synoptic timescales, when following warm and stable conditions latent heat flux was low and dominated by higher fractionation for a few days. We hypothesize that the shifting from negligible to large isotopic fractionation reflects the development and subsequent exhaustion of liquid water on the surface. The results illustrate the complex and nonlinear controls on isotopic fractionation from icy lakes, which implies that the isotopic enrichment from ablation could vary significantly over timescales relevant for changing lake volumes. Future work using water isotope fluxes for longer periods of time and over additional perennial and seasonal ice-covered lake systems is critical for developing models of the isotopic mass balance of arctic and Antarctic lake systems.

10aDry Valley lakes10aisotope fractionation10astable water isotopes1 aBellagamba, Anthony, W.1 aBerkelhammer, Max1 aWinslow, Luke, A.1 aDoran, Peter, T.1 aMyers, Krista, F.1 aDevlin, Shawn1 aHawes, Ian uhttps://www.tandfonline.com/doi/full/10.1080/15230430.2021.200189902329nas a2200193 4500008004100000022001400041245010500055210006900160260001200229300001200241490000700253520164000260100002301900700002601923700001901949700002201968700002601990856011902016 2021 eng d a1527-140400aMeta-analysis of Cryogenian through modern quartz microtextures reveals sediment transport histories0 aMetaanalysis of Cryogenian through modern quartz microtextures r c09/2021 a929-9440 v913 a

Quantitative analysis of quartz microtextures by means of scanning electron microscopy (SEM) can reveal the transport histories of modern and ancient sediments. However, because workers identify and count microtextures differently, it is difficult to directly compare quantitative microtextural data analyzed by different workers. As a result, the defining microtextures of certain transport modes and their probabilities of occurrence are not well constrained. We used principal-component analysis (PCA) to directly compare modern and ancient aeolian, fluvial, and glacial samples from the literature with nine new samples from active aeolian and glacial environments. Our results demonstrate that PCA can group microtextural samples by transport mode and differentiate between aeolian transport and fluvial and glacial transport across studies. The PCA ordination indicates that aeolian samples are distinct from fluvial and glacial samples, which are in turn difficult to disambiguate from each other. Ancient and modern sediments are also shown to have quantitatively similar microtextural relationships. Therefore, PCA may be a useful tool to constrain the ambiguous transport histories of some ancient sediment grains. As a case study, we analyzed two samples with ambiguous transport histories from the Cryogenian Bråvika Member (Svalbard). Integrating PCA with field observations, we find evidence that the Bråvika Member facies investigated here includes aeolian deposition and may be analogous to syn-glacial Marinoan aeolian units including the Bakoye Formation in Mali and the Whyalla Sandstone in South Australia.

1 aReahl, Jocelyn, N.1 aCantine, Marjorie, D.1 aWilcots, Julia1 aMackey, Tyler, J.1 aBergmann, Kristin, D. uhttps://pubs.geoscienceworld.org/jsedres/article/91/9/929/607764/Meta-analysis-of-Cryogenian-through-modern-quartz02224nas a2200229 4500008004100000245016400041210006900205260001200274300000800286490000700294520144700301100002401748700002301772700002201795700001501817700002101832700002201853700002201875700003201897700002101929856004401950 2021 eng d00aMetabolic capacity of the Antarctic cyanobacterium Phormidium pseudopriestleyi that sustains oxygenic photosynthesis in the presence of hydrogen sulfide0 aMetabolic capacity of the Antarctic cyanobacterium IPhormidium p c03/2021 a4260 v123 a

Sulfide inhibits oxygenic photosynthesis by blocking electron transfer between H2O and the oxygen-evolving complex in the D1 protein of Photosystem II. The ability of cyanobacteria to counter this effect has implications for understanding the productivity of benthic microbial mats in sulfidic environments throughout Earth history. In Lake Fryxell, Antarctica, the benthic, filamentous cyanobacterium Phormidium pseudopriestleyi creates a 1–2 mm thick layer of 50 µmol L−1 O2 in otherwise sulfidic water, demonstrating that it sustains oxygenic photosynthesis in the presence of sulfide. A metagenome-assembled genome of P. pseudopriestleyi indicates a genetic capacity for oxygenic photosynthesis, including multiple copies of psbA (encoding the D1 protein of Photosystem II), and anoxygenic photosynthesis with a copy of sqr (encoding the sulfide quinone reductase protein that oxidizes sulfide). The genomic content of P. pseudopriestleyi is consistent with sulfide tolerance mechanisms including increasing psbA expression or directly oxidizing sulfide with sulfide quinone reductase. However, the ability of the organism to reduce Photosystem I via sulfide quinone reductase while Photosystem II is sulfide-inhibited, thereby performing anoxygenic photosynthesis in the presence of sulfide, has yet to be demonstrated. 

1 aLumian, Jessica, E.1 aJungblut, Anne, D.1 aDillon, Megan, L.1 aHawes, Ian1 aDoran, Peter, T.1 aMackey, Tyler, J.1 aDick, Gregory, J.1 aGrettenberger, Christen, L.1 aSumner, Dawn, Y. uhttps://www.mdpi.com/2073-4425/12/3/42601817nas a2200169 4500008004100000245017100041210006900212260001200281300001300293490000700306520116600313100002601479700002001505700002001525700002401545856007801569 2021 eng d00aResearch sites get closer to field camps over time: Informing environmental management through a geospatial analysis of science in the McMurdo Dry Valleys, Antarctica0 aResearch sites get closer to field camps over time Informing env c11/2021 ae02579500 v163 a

As in many parts of the world, the management of environmental science research in Antarctica relies on cost-benefit analysis of negative environmental impact versus positive scientific gain. Several studies have examined the environmental impact of Antarctic field camps, but very little work looks at how the placement of these camps influences scientific research. In this study, we integrate bibliometrics, geospatial analysis, and historical research to understand the relationship between field camp placement and scientific production in the McMurdo Dry Valleys of East Antarctica. Our analysis of the scientific corpus from 1907–2016 shows that, on average, research sites have become less dispersed and closer to field camps over time. Scientific output does not necessarily correspond to the number of field camps, and constructing a field camp does not always lead to a subsequent increase in research in the local area. Our results underscore the need to consider the complex historical and spatial relationships between field camps and research sites in environmental management decision-making in Antarctica and other protected areas.

1 aChignell, Stephen, M.1 aMyers, Madeline1 aHowkins, Adrian1 aFountain, Andrew, G uhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.025795003475nas a2200217 4500008004100000245017000041210006900211260003900280300000800319490000800327520273200335653001603067653000803083653001703091653001903108653002003127653001703147100001603164700002903180856004803209 2021 eng d00aRole of cyclic electron flow (CEF) and photosystem I (PSI) supercomplex formation during acclimation to long-term salinity stress in green algae: A comparative study0 aRole of cyclic electron flow CEF and photosystem I PSI supercomp aOxford, OHbMiami Universityc2021 a2360 vPhD3 aPhotosynthesis is one of the most important processes on Earth by which organisms convert solar energy into usable forms of energy. Linear electron flow (LEF) and cyclic electron flow (CEF) constitute two major pathways in photosynthesis. While LEF leads to production of both ATP and NADPH, CEF only produces ATP that helps balance the ATP:NADPH ratio required for carbon fixation. CEF also plays a major role during acclimation to several environmental stressors. However, the regulation and mechanism by which CEF operates is still not clearly understood. Recent studies have shown that formation of a protein supercomplex with PSI appears to be essential for induction of CEF in several model organisms. However, both supercomplex formation and CEF induction have been mainly studied under short-term, transitory stress conditions. In addition, the role and mechanism by which organisms may rely on CEF to survive in their natural habitat and acclimate to stress over a long period of time has not been considered. In this study we compared how three photosynthetic organisms (one model alga, Chlamydomonas reinhardtii; two extremophiles, C. sp. UWO241 and C. sp. ICE-MDV) utilize CEF to cope with their natural environment and adapt to steady-state environmental stress. To that end, the objectives of this thesis were i) to elucidate the role of CEF in long-term salinity acclimation ii) to understand the downstream changes associated with increased CEF, and iii) to identify whether PSI-supercomplexes are associated with increased CEF during salinity acclimation. We hypothesized that a stable PSI-supercomplex is required for high CEF, which in turn supports strong carbon fixation capacity for production of downstream metabolic products important for long-term acclimation to salinity stress. We showed for the first time, that increased CEF in UWO241 leads to excess ATP production and rewiring of downstream metabolism under high salinity. Next, we showed that a laboratory evolved salinity-tolerant strain of model C. reinhardtii uses constitutive upregulation of CEF to deal with salinity stress, which is in-turn associated with increased non-photochemical quenching and rewired carbon metabolism. Last, we show that CEF is involved in salinity acclimation in all three Chlamydomonas species, regardless of their salinity tolerance. We also show that PSI-supercomplexes are associated with increased CEF in these species. Characterization of high-salt supercomplex of C. reinhardtii revealed that it shares many similarities with the extensively described state 2 supercomplex, and that supercomplex composition might be species dependent rather than stress dependent.10aacclimation10aCEF10aextremophile10aPhotosynthesis10asalinity stress10asupercomplex1 aKalra, Isha1 aMorgan-Kiss, Rachael, M. uhttps://www.proquest.com/docview/257256058502603nas a2200217 4500008004100000022001400041245012400055210006900179260001200248520186200260653001502122653002702137653001902164653002102183653001502204100002202219700002502241700002402266700002602290856006902316 2021 eng d a2169-895300aThe role of hyporheic connectivity in determining nitrogen availability: Insights from an intermittent Antarctic stream0 arole of hyporheic connectivity in determining nitrogen availabil c04/20223 a

Due to widespread manipulation of nitrogen (N), much research has focused on processes controlling the fate of anthropogenic N in streams. Yet, in a variety of oligotrophic systems, N fixed by periphyton is a significant driver of ecosystem metabolism. Due to difficulties partitioning allochthonous and autochthonous sources, there is limited information regarding how the latter is processed. Autochthonous N may be particularly important in alpine, arid, or polar environments. We test the hypothesis that the availability of remineralized autochthonous N is controlled by connectivity between the hyporheic zone and main channel due to the contrasting biogeochemical functions of benthic autotrophs (including N‐fixing Nostoc) and hyporheic heterotrophs in an intermittent Antarctic stream. There, we collected surface water and hyporheic water concurrently at 4‐6 hour intervals over a 32.5‐hr period during one flow season and opportunistically throughout a second. Hyporheic water had 7 to 30 times greater nitrate‐N concentrations relative to surface water across all flow conditions. In contrast, ammonium concentrations were generally lower, although similar among locations. Additionally, nitrate in hyporheic water was positively correlated with silica, an indicator of hyporheic residence time. A laboratory assay confirmed prior inferences that hyporheic microbial communities possess the functional potential to perform nitrification. Together, these findings suggest that remineralized autochthonous N accumulates in the hyporheic zone even as streamflow varies and likely subsidizes stream N availability—which supports prior inferences from N stable isotope data at this site. These results highlight the importance of hyporheic connectivity in controlling autochthonous N cycling and availability in streams.

10aAntarctica10aautochthonous nitrogen10ahyporheic zone10anitrogen cycling10astreamflow1 aSingley, Joel, G.1 aGooseff, Michael, N.1 aMcKnight, Diane, M.1 aHinckley, Eve-Lyn, S. uhttps://agupubs.onlinelibrary.wiley.com/doi/10.1029/2021JG00630903499nas a2200229 4500008004100000245016600041210006900207260001200276300001100288490000700299520269000306653002302996653001503019653004103034653001603075653002403091653002003115100002203135700002203157700002403179856006603203 2021 eng d00aSupporting simultaneous air revitalization and thermal control in a crewed habitat with temperate Chlorella vulgaris and eurythermic Antarctic Chlorophyta0 aSupporting simultaneous air revitalization and thermal control i c08/2021 a7097460 v123 a

Including a multifunctional, bioregenerative algal photobioreactor for simultaneous air revitalization and thermal control may aid in carbon loop closure for long-duration surface habitats. However, using water-based algal media as a cabin heat sink may expose the contained culture to a dynamic, low temperature environment. Including psychrotolerant microalgae, native to these temperature regimes, in the photobioreactor may contribute to system stability. This paper assesses the impact of a cycled temperature environment, reflective of spacecraft thermal loops, to the oxygen provision capability of temperate Chlorella vulgaris and eurythermic Antarctic Chlorophyta. The tested 28-min temperature cycles reflected the internal thermal control loops of the International Space Station (C. vulgaris, 9–27°C; Chlorophyta-Ant, 4–14°C) and included a constant temperature control (10°C). Both sample types of the cycled temperature condition concluded with increased oxygen production rates (C. vulgaris; initial: 0.013 mgO2 L-1, final: 3.15 mgO2 L–1 and Chlorophyta-Ant; initial: 0.653 mgO2 L–1, final: 1.03 mgO2 L–1) and culture growth, suggesting environmental acclimation. Antarctic sample conditions exhibited increases or sustainment of oxygen production rates normalized by biomass dry weight, while both C. vulgaris sample conditions decreased oxygen production per biomass. However, even with the temperature-induced reduction, cycled temperature C. vulgaris had a significantly higher normalized oxygen production rate than Antarctic Chlorophyta. Chlorophyll fluorometry measurements showed that the cycled temperature conditions did not overly stress both sample types (FV/FM: 0.6–0.75), but the Antarctic Chlorophyta sample had significantly higher fluorometry readings than its C. vulgaris counterpart (F = 6.26, P < 0.05). The steady state C. vulgaris condition had significantly lower fluorometry readings than all other conditions (FV/FM: 0.34), suggesting a stressed culture. This study compares the results to similar experiments conducted in steady state or diurnally cycled temperature conditions. Recommendations for surface system implementation are based off the presented results. The preliminary findings imply that both C. vulgaris and Antarctic Chlorophyta can withstand the dynamic temperature environment reflective of a thermal control loop and these data can be used for future design models.

10aair revitalization10aAntarctica10abioregenerative life support systems10aChlorophyta10aMcMurdo Dry Valleys10athermal control1 aMatula, Emily, E.1 aNabity, James, A.1 aMcKnight, Diane, M. uhttps://www.frontiersin.org/article/10.3389/fmicb.2021.70974602152nas a2200253 4500008004100000245012600041210006900167260001200236300001600248490000700264520133900271100002201610700002101632700002501653700002001678700002201698700001701720700002201737700002201759700001901781700002201800700002301822856005301845 2021 eng d00aThermal legacy of a large paleolake in Taylor Valley, East Antarctica, as evidenced by an airborne electromagnetic survey0 aThermal legacy of a large paleolake in Taylor Valley East Antarc c08/2021 a3577 - 35930 v153 a

Previous studies of the lakes of the McMurdo Dry Valleys have attempted to constrain lake level history, and results suggest the lakes have undergone hundreds of meters of lake level change within the last 20 000 years. Past studies have utilized the interpretation of geologic deposits, lake chemistry, and ice sheet history to deduce lake level history; however a substantial amount of disagreement remains between the findings, indicating a need for further investigation using new techniques. This study utilizes a regional airborne resistivity survey to provide novel insight into the paleohydrology of the region. Mean resistivity maps revealed an extensive brine beneath the Lake Fryxell basin, which is interpreted as a legacy groundwater signal from higher lake levels in the past. Resistivity data suggest that active permafrost formation has been ongoing since the onset of lake drainage and that as recently as 1500–4000 years BP, lake levels were over 60 m higher than present. This coincides with a warmer-than-modern paleoclimate throughout the Holocene inferred by the nearby Taylor Dome ice core record. Our results indicate Mid to Late Holocene lake level high stands, which runs counter to previous research finding a colder and drier era with little hydrologic activity throughout the last 5000 years.

1 aMyers, Krista, F.1 aDoran, Peter, T.1 aTulaczyk, Slawek, M.1 aFoley, Neil, T.1 aBording, Thue, S.1 aAuken, Esben1 aDugan, Hilary, A.1 aMikucki, Jill, A.1 aFoged, Nikolaj1 aGrombacher, Denys1 aVirginia, Ross, A. uhttps://tc.copernicus.org/articles/15/3577/2021/02753nas a2200325 4500008004100000022001400041245011500055210007300170260001200243300001800255490000800273520178400281653001002065653001402075653001602089653000802105653001002113653001002123653001402133653001102147100002802158700002102186700003302207700002202240700002202262700002402284700002902308700002102337856006902358 2021 eng d a2169-895300aYear‐round and long‐term phytoplankton dynamics in Lake Bonney, a permanently ice‐covered Antarctic lake0 aYear‐round and long‐term phytoplankton dynamics in Lake Bonney a c04/2021 ae2020JG0059250 v1263 a

Lake Bonney (McMurdo Dry Valleys, east Antarctica) represents a year‐round refugium for life adapted to permanent extreme conditions. Despite intensive research since the 1960s, due to the logistical constraints posed by 4‐months of 24‐h darkness, knowledge of how the resident photosynthetic microorganisms respond to the polar winter is limited. In addition, the lake level has risen by more than 3 m since 2004: impacts of rapid lake level rise on phytoplankton community structure is also poorly understood. From 2004 to 2015 an in situ submersible spectrofluorometer (bbe FluoroProbe) was deployed in Lake Bonney during the austral summer to quantify the vertical structure of four functional algal groups (green algae, mixed algae, and cryptophytes, cyanobacteria). During the 2013–2014 field season the Fluoroprobe was mounted on autonomous cable‐crawling profilers deployed in both the east and west lobes of Lake Bonney, obtaining the first daily phytoplankton profiles through the polar night. Our findings showed that phytoplankton communities were differentially impacted by physical and chemical factors over long‐term versus seasonal time scales. Following a summer of rapid lake level rise (2010–2011), an increase in depth integrated chlorophyll a (chl‐a) occurred in Lake Bonney caused by stimulation of photoautotrophic green algae. Conversely, peaks in chl‐a during the polar night were associated with an increase in mixotrophic haptophytes and cryptophytes. Collectively our data reveal that phytoplankton groups possessing variable trophic abilities are differentially competitive during seasonal and long‐term time scales owing to periods of higher nutrients (photoautotrophs) versus light/energy limitation (mixotrophs).

10aalgae10aAntarctic10afluorometry10aice10alakes10alight10aprofiling10awinter1 aPatriarche, Jeffrey, D.1 aPriscu, John, C.1 aTakacs-Vesbach, Cristina, D.1 aWinslow, Luke, A.1 aMyers, Krista, F.1 aBuelow, Heather, N.1 aMorgan-Kiss, Rachael, M.1 aDoran, Peter, T. uhttps://agupubs.onlinelibrary.wiley.com/doi/10.1029/2020JG00592504249nas a2200253 4500008004100000245009000041210006900131260001200200490000600212520350100218653002003719653002003739653001303759100001903772700002003791700002203811700002403833700002503857700001903882700001203901700001703913700002103930856004403951 2020 eng d00aChange in Pictures: Creating best practices in archiving ecological imagery for reuse0 aChange in Pictures Creating best practices in archiving ecologic c09/20200 v43 a

The research data repository of the Environmental Data Initiative (EDI) is building on over 30 years of data curation research and experience in the National Science Foundation-funded US Long-Term Ecological Research (LTER) Network. It provides mature functionalities, well established workflows, and now publishes all ‘long-tail’ environmental data. High quality scientific metadata are enforced through automatic checks against community developed rules and the Ecological Metadata Language (EML) standard. Although the EDI repository is far along in making its data findable, accessible, interoperable, and reusable (FAIR), representatives from EDI and the LTER are developing best practices for the edge cases in environmental data publishing. One of these is the vast amount of imagery taken in the context of ecological research, ranging from wildlife camera traps to plankton imaging systems to aerial photography. Many images are used in biodiversity research for community analyses (e.g., individual counts, species cover, biovolume, productivity), while others are taken to study animal behavior and landscape-level change.

Some examples from the LTER Network include: using photos of a heron colony to measure provisioning rates for chicks (Clarkson and Erwin 2018) or identifying changes in plant cover and functional type through time (Peters et al. 2020). Multi-spectral images are employed to identify prairie species. Underwater photo quads are used to monitor changes in benthic biodiversity (Edmunds 2015). Sosik et al. (2020) used a continuous Imaging FlowCytobot to identify and measure phyto- and microzooplankton. Cameras at McMurdo Dry Valleys assess snow and ice cover on Antarctic lakes allowing estimation of primary production (Myers 2019).

It has been standard practice to publish numerical data extracted from images in EDI; however, the supporting imagery generally has not been made publicly available. Our goal in developing best practices for documenting and archiving these images is for them to be discovered and re-used. Our examples demonstrate several issues. The research questions, and hence, the image subjects are variable. Images frequently come in logical sets of time series. The size of such sets can be large and only some images may be contributed to a dedicated specialized repository. Finally, these images are taken in a larger monitoring context where many other environmental data are collected at the same time and location.

Currently, a typical approach to publishing image data in EDI are packages containing compressed (ZIP or tar) files with the images, a directory manifest with additional image-specific metadata, and a package-level EML metadata file. Images in the compressed archive may be organized within directories with filenames corresponding to treatments, locations, time periods, individuals, or other grouping attributes. Additionally, the directory manifest table has columns for each attribute. Package-level metadata include standard coverage elements (e.g., date, time, location) and sampling methods. This approach of archiving logical ‘sets’ of images reduces the effort of providing metadata for each image when most information would be repeated, but at the expense of not making every image individually searchable. The latter may be overcome if the provided manifest contains standard metadata that would allow searching and automatic integration with other images.

10adata repository10aecological data10ametadata1 aGries, Corinna1 aBeaulieu, Stace1 aBrown, Renée, F.1 aGastil-Buhl, Gastil1 aElmendorf, Sarah, C.1 aHsieh, Hsun-Yi1 aKui, Li1 aMaurer, Greg1 aPorter, John, H. uhttps://biss.pensoft.net/article/59082/03380nas a2200205 4500008004100000245009700041210006900138260005400207490001000261520267200271653001002943653001402953653002902967653002202996653002603018653003203044100002603076700002403102856004803126 2020 eng d00aCharacterization of spatial and environmental influences on stream diatoms and cyanobacteria0 aCharacterization of spatial and environmental influences on stre aBoulder, CObUniversity of Colorado Boulderc20200 vPh.D.3 a

Primary producing algae form the basis of carbon fixation, oxygen production, and food webs in aquatic ecosystems globally. However, human activities disrupt climate and freshwater physicochemistry. These impacts alter the health of algal communities and the ecosystem services algae provide. Meanwhile, spatial processes like dispersal and landscape characteristics like geology also influence algal structure and function. Diatoms are indicators of stream health and are model organisms for understanding the processes underlying microbial biogeography. Benthic cyanobacteria present risks to human health through the proliferation of toxin-producing blooms. With this dissertation, I investigate the ecosystem processes that influence diatom and cyanobacterial community composition and taxon distributions. My goal is to advance the understanding of ecosystem controls on algal biogeography and to characterize taxon-specific autecology for use in environmental management. First, I measured the extent of wind-mediated dispersal of benthic diatoms across aquatic habitats to better understand how community composition is structured by spatial processes across the McMurdo Dry Valleys polar desert in Antarctica. I found that inter-habitat dispersal is common but less influential on community composition than intra-habitat factors such as environmental conditions. I then used non-linear, multivariable modeling to assess the relative influences of climate, watershed characteristics, and in-stream stressors on the relative abundances of 268 diatom taxa across gradients of human impact in the northeast United States. My results indicate diatom taxa are affected by different suites of environmental conditions but that taxa belong to ecological guilds based on shared responsiveness to environmental factors. Finally, I applied multivariable modeling towards understanding the effects of aquatic stressors, including herbicides and persistent organic pollutants, on the distributions of benthic cyanobacteria across northeast U.S. streams. I found that watershed characteristics, streamflow, and herbicides were more influential than light availability, water temperature, and nutrients on the distributions of potentially toxigenic cyanobacterial genera. Collectively, this research expands the knowledge of how benthic algal communities and taxon distributions are structured at large spatial scales along gradients of unimpacted and human-altered environmental conditions. I provide a novel modeling framework and taxon-specific autecological information that can be applied to environmental assessments of stream health and future algal research.

10aalgae10adispersal10aenvironmental assessment10ahuman disturbance10ametacommunity ecology10aspecies distribution models1 aSchulte, Nicholas, O.1 aMcKnight, Diane, M. uhttps://www.proquest.com/docview/247621626302207nas a2200205 4500008004100000245010800041210006900149260001200218520152500230100001601755700001401771700002301785700001901808700002201827700001401849700001901863700002101882700002901903856006901932 2020 eng d00aChlamydomonas sp. UWO 241 exhibits high cyclic electron flow and rewired metabolism under high salinity0 aChlamydomonas sp UWO 241 exhibits high cyclic electron flow and  c03/20203 a

The Antarctic green alga Chlamydomonas sp. UWO 241 (UWO 241) is adapted to permanent low temperatures, hypersalinity, and extreme shade. one of the most striking phenotypes of UWO 241 is an altered photosystem I (PSI) organization and constitutive PSI cyclic electron flow (CEF). To date, little attention has been paid to CEF during long-term stress acclimation, and the consequences of sustained CEF in UWO 241 are not known. In this study, we combined photobiology, proteomics, and metabolomics to understand the underlying role of sustained CEF in high salinity stress acclimation. High salt-grown UWO 241 exhibited increased thylakoid proton motive flux and an increased capacity for non-photochemical quenching. Under high salt, a significant proportion of the upregulated enzymes were associated with the Calvin Benson Bassham Cycle, carbon storage metabolism, and protein translation. Two key enzymes of the Shikimate pathway, DAHP synthase and chorismate synthase, were also upregulated, as well as indole-3-glycerol phosphate synthase, an enzyme involved in the biosynthesis of L-tryptophan and indole acetic acid. In addition, several compatible solutes (glycerol, proline, and sucrose) accumulated to high levels in high salt-grown UWO 241 cultures. We suggest that UWO 241 maintains constitutively high CEF through the associated PSI-cytochrome b6f supercomplex to support robust growth and strong photosynthetic capacity under a constant growth regime of low temperatures and high salinity.

1 aKalra, Isha1 aWang, Xin1 aCvetkovska, Marina1 aJeong, Jooyeon1 aMcHargue, William1 aZhang, Ru1 aHüner, Norman1 aYuan, Joshua, S.1 aMorgan-Kiss, Rachael, M. uhttp://www.plantphysiol.org/content/early/2020/04/03/pp.19.0128001864nas a2200193 4500008004100000245012800041210006900169260001200238520119500250653002401445653001801469653002501487100001801512700001801530700002101548700001401569700001801583856006901601 2020 eng d00aClimate from the McMurdo Dry Valleys, Antarctica, 1986 – 2017: Surface air temperature trends and redefined summer season0 aClimate from the McMurdo Dry Valleys Antarctica 1986 2017 Surfac c05/20203 a

The weather of the McMurdo Dry Valleys, Antarctica, the largest ice‐free region of the Antarctica, has been continuously monitored since 1985 with currently 14 operational meteorological stations distributed throughout the valleys. Because climate is based on a 30‐year record of weather, this is the first study to truly define the contemporary climate of the McMurdo Dry Valleys. Mean air temperature and solar radiation based on all stations were ‐20°C and 102 W m‐2, respectively. Depending on the site location, the mean annual air temperatures on the valleys floors ranged between ‐15°C and ‐30°C, and mean annual solar radiation varied between 72 W m‐2 and 122 W m‐2. Surface air temperature decreased by 0.7°C per decade from 1986 to 2006 at Lake Hoare station (longest continuous record), after which the record is highly variable with no trend. All stations with sufficiently long records showed similar trend shifts in 2005 ±1 year. Summer is defined as November through February, using a physically based process: up‐valley warming from the coast associated with a change in atmospheric stability.

10aMcMurdo Dry Valleys10asummer season10aweather observations1 aObryk, M., K.1 aDoran, P., T.1 aFountain, A., G.1 aMyers, M.1 aMcKay, C., P. uhttps://agupubs.onlinelibrary.wiley.com/doi/10.1029/2019JD03218002314nas a2200265 4500008004100000245012500041210006900166260001200235490000700247520147000254653001501724653001101739653001701750653001801767653001101785653004001796100002201836700001501858700002301873700002201896700002401918700002101942700002101963856006401984 2020 eng d00aEnergetic and environmental constraints on the community structure of benthic microbial mats in Lake Fryxell, Antarctica0 aEnergetic and environmental constraints on the community structu c02/20200 v963 a

Ecological communities are regulated by the flow of energy through environments. Energy flow is typically limited by access to photosynthetically active radiation (PAR) and oxygen concentration (O2). The microbial mats growing on the bottom of Lake Fryxell, Antarctica, have well-defined environmental gradients in PAR and (O2). We analyzed the metagenomes of layers from these microbial mats to test the extent to which access to oxygen and light controls community structure. We found variation in the diversity and relative abundances of Archaea, Bacteria and Eukaryotes across three (O2) and PAR conditions: high (O2) and maximum PAR, variable (O2) with lower maximum PAR, and low (O2) and maximum PAR. We found distinct communities structured by the optimization of energy use on a millimeter-scale across these conditions. In mat layers where (O2) was saturated, PAR structured the community. In contrast, (O2) positively correlated with diversity and affected the distribution of dominant populations across the three habitats, suggesting that meter-scale diversity is structured by energy availability. Microbial communities changed across covarying gradients of PAR and (O2). The comprehensive metagenomic analysis suggests that the benthic microbial communities in Lake Fryxell are structured by energy flow across both meter- and millimeter-scales.

10aAntarctica10aenergy10aLake Fryxell10amicrobial mat10aOxygen10aPhotosynthetically Active Radiation1 aDillon, Megan, L.1 aHawes, Ian1 aJungblut, Anne, D.1 aMackey, Tyler, J.1 aEisen, Jonathan, A.1 aDoran, Peter, T.1 aSumner, Dawn, Y. uhttps://academic.oup.com/femsec/article/96/2/fiz207/569719602205nas a2200205 4500008004100000245012400041210006900165260001200234300001300246490000700259520150700266100002201773700001501795700002301810700002201833700002401855700002101879700002101900856007801921 2020 eng d00aEnvironmental control on the distribution of metabolic strategies of benthic microbial mats in Lake Fryxell, Antarctica0 aEnvironmental control on the distribution of metabolic strategie c04/2020 ae02310530 v153 a

Ecological theories posit that heterogeneity in environmental conditions greatly affects community structure and function. However, the degree to which ecological theory developed using plant- and animal-dominated systems applies to microbiomes is unclear. Investigating the metabolic strategies found in microbiomes are particularly informative for testing the universality of ecological theories because microorganisms have far wider metabolic capacity than plants and animals. We used metagenomic analyses to explore the relationships between the energy and physicochemical gradients in Lake Fryxell and the metabolic capacity of its benthic microbiome. Statistical analysis of the relative abundance of metabolic marker genes and gene family diversity shows that oxygenic photosynthesis, carbon fixation, and flavin-based electron bifurcation differentiate mats growing in different environmental conditions. The pattern of gene family diversity points to the likely importance of temporal environmental heterogeneity in addition to resource gradients. Overall, we found that the environmental heterogeneity of photosynthetically active radiation (PAR) and oxygen concentration ([O2]) in Lake Fryxell provide the framework by which metabolic diversity and composition of the community is structured, in accordance with its phylogenetic structure. The organization of the resulting microbial ecosystems are consistent with the maximum power principle and the species sorting model.

1 aDillon, Megan, L.1 aHawes, Ian1 aJungblut, Anne, D.1 aMackey, Tyler, J.1 aEisen, Jonathan, A.1 aDoran, Peter, T.1 aSumner, Dawn, Y. uhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.023105303261nas a2200241 4500008004100000245013300041210006900174260001200243490000600255520247400261653001502735653002202750653001402772653001102786653001902797100002002816700002202836700002202858700002402880700002402904700002102928856007002949 2020 eng d00aEvaluating alternative metacommunity hypotheses for diatoms in the McMurdo Dry Valleys using simulations and remote sensing data0 aEvaluating alternative metacommunity hypotheses for diatoms in t c09/20200 v83 a

Diatoms are diverse and widespread freshwater Eukaryotes that make excellent microbial subjects for addressing questions in metacommunity ecology. In the McMurdo Dry Valleys of Antarctica, the simple trophic structure of glacier-fed streams provides an ideal outdoor laboratory where well-described diatom assemblages are found within two cyanobacterial mat types, which occupy different habitats and vary in coverage within and among streams. Specifically, black mats of Nostoc spp. occur in marginal wetted habitats, and orange mats (Oscillatoria spp. and Phormidium spp.) occur in areas of consistent stream flow. Despite their importance as bioindicators for changing environmental conditions, the role of dispersal in structuring dry valley diatom metacommunities remains unclear. Here, we use MCSim, a spatially explicit metacommunity simulation package for R, to test alternative hypotheses about the roles of dispersal and species sorting in maintaining the biodiversity of diatom assemblages residing in black and orange mats. The spatial distribution and patchiness of cyanobacterial mat habitats was characterized by remote imagery of the Lake Fryxell sub-catchment in Taylor Valley. The available species pool for diatom metacommunity simulation scenarios was informed by the Antarctic Freshwater Diatoms Database, maintained by the McMurdo Dry Valleys Long Term Ecological Research program. We used simulation outcomes to test the plausibility of alternative community assembly hypotheses to explain empirically observed patterns of freshwater diatom biodiversity in the long-term record. The most plausible simulation scenarios suggest species sorting by environmental filters, alone, was not sufficient to maintain biodiversity in the Fryxell Basin diatom metacommunity. The most plausible scenarios included either (1) neutral models with different immigration rates for diatoms in orange and black mats or (2) species sorting by a relatively weak environmental filter, such that dispersal dynamics also influenced diatom community assembly, but there was not such a strong disparity in immigration rates between mat types. The results point to the importance of dispersal for understanding current and future biodiversity patterns for diatoms in this ecosystem, and more generally, provide further evidence that metacommunity theory is a useful framework for testing hypotheses about microbial community assembly.

10aAntarctica10aBacillariophyceae10adispersal10aNostoc10astream ecology1 aSokol, Eric, R.1 aBarrett, John, E.1 aKohler, Tyler, J.1 aMcKnight, Diane, M.1 aSalvatore, Mark, R.1 aStanish, Lee, F. uhttps://www.frontiersin.org/article/10.3389/fevo.2020.521668/full01971nas a2200181 4500008004100000245008500041210006900126260005200195520137400247100002101621700002101642700001801663700002101681700001301702700002001715700002001735856003401755 2020 eng d00aFollowing the Astrobiology Roadmap: Origins, Habitability and Future Exploration0 aFollowing the Astrobiology Roadmap Origins Habitability and Futu aNorfolk, United KingdombCaister Academic Press3 a

Astrobiology asks three fundamental questions as outlined by the NASA Astrobiology Roadmap: 1. How did Life begin and evolve?; Is there Life elsewhere in the Universe?; and, What is the future of Life on Earth? As we gain perspective on how Life on Earth arose and adapted to its many niches, we too gain insight into how a planet achieves habitability. Here on Earth, microbial Life has evolved to exist in a wide range of habitats from aquatic systems to deserts, the human body, and the International Space Station (ISS). Landers, rovers, and orbiter missions support the search for signatures of Life beyond Earth, by generating data on surface and subsurface conditions of other worlds. These have provided evidence for water activity, supporting the potential for extinct or extant Life. To investigate the putative ecologies of these systems, we study extreme environments on Earth. Several locations on our planet provide analog settings to those we have detected or expect to find on neighboring and distant worlds. Whereas, the field of space biology uses the ISS and low gravity analogs to gain insight on how transplanted Earth-evolved organisms will respond to extraterrestrial environments. Modern genomics allows us to chronicle the genetic makeup of such organisms and provides an understanding of how Life adapts to various extreme environments.

1 aO'Rourke, Aubrie1 aZoumplis, Angela1 aWilburn, Paul1 aLee, Michael, D.1 aLee, Zhi1 aVecina, Marissa1 aMercader, Kysha uhttps://www.caister.com/astro02540nas a2200289 4500008004100000245012600041210006900167260001200236490000800248520163000256653002101886653001501907653001701922653001501939653002401954653001901978100002101997700002102018700001902039700002402058700001902082700001702101700002402118700001902142700001702161856007202178 2020 eng d00aGeochemistry of aeolian material from the McMurdo Dry Valleys, Antarctica: Insights into Southern Hemisphere dust sources0 aGeochemistry of aeolian material from the McMurdo Dry Valleys An c10/20200 v5473 a

In the Southern Hemisphere, the major sources of dust and other aeolian materials are from Patagonia, South Africa, Australia, and New Zealand. Dust from Patagonia and New Zealand has been identified in ice cores throughout Antarctica, suggesting that during arid and windy periods, such as glacial periods, dust can be entrained and transported onto the continent. However, little information exists on modern Antarctic dust sources, transport, and its role in the Southern Hemisphere dust cycle. We present the first geochemical characterization of aeolian materials collected at five heights (between 5 cm and 100 cm) above the surface in four valleys within the McMurdo Dry Valleys, the largest ice-free area in Antarctica. Our mineralogy data indicate that these materials are primarily derived from local rocks of the McMurdo Volcanics, Ferrar Dolerite, Beacon Sandstone and Granite Harbor Intrusives, with varying contributions of each rock type dependent on the valley location. While major oxide, trace element and rare earth element data show that low elevation and coastal locations (with respect to the Ross Sea) are dominated by local sources, high elevation and inland locations have accumulated both local materials and dust from other distant Southern Hemisphere sources. This far-traveled material may not be accumulating today, but represents a paleo source that is resuspended from the soils. By geochemically “fingerprinting” aeolian materials from the MDV, we can better inform future studies on the transport of materials within Antarctica and between Southern Hemisphere land masses.

10aaeolian material10aAntarctica10amajor oxides10amineralogy10arare earth elements10atrace elements1 aDiaz, Melisa, A.1 aWelch, Susan, A.1 aSheets, J., M.1 aWelch, Kathleen, A.1 aKhan, Alia, L.1 aAdams, Byron1 aMcKnight, Diane, M.1 aCary, Craig, S1 aLyons, Berry uhttps://www.sciencedirect.com/science/article/pii/S0012821X2030404002119nas a2200217 4500008004100000245007600041210006900117260001200186520131800198653001101516653003001527653002801557653002201585653002401607653001201631653001801643100002401661700002101685700002001706856017501726 2020 eng d00aGIS tool to predict photosynthetically active radiation in a Dry Valley0 aGIS tool to predict photosynthetically active radiation in a Dry c04/20203 a

Understanding primary productivity is a core research area of the National Science Foundation's Long-Term Ecological Research Network. This study presents the development of the GIS-based Topographic Solar Photosynthetically Active Radiation (T-sPAR) toolbox for Taylor Valley. It maps surface photosynthetically active radiation using four meteorological stations with ~20 years of data. T-sPAR estimates were validated with ground-truth data collected at Taylor Valley's major lakes during the 2014–15 and 2015–16 field seasons. The average daily error ranges from 0.13 mol photons m-2 day-1 (0.6%) at Lake Fryxell to 3.8 mol photons m-2 day-1 (5.8%) at Lake Hoare. We attribute error to variability in terrain and sun position. Finally, a user interface was developed in order to estimate total daily surface photosynthetically active radiation for any location and date within the basin. T-sPAR improves upon existing toolboxes and models by allowing for the inclusion of a statistical treatment of light attenuation due to cloud cover. The T-sPAR toolbox could be used to inform biological sampling sites based on radiation distribution, which could collectively improve estimates of net primary productivity, in some cases by up to 25%.

10aArcMap10aautomated weather station10adigital elevation model10aice-covered lakes10aMcMurdo Dry Valleys10aR model10aTaylor Valley1 aAcosta, Dimitri, R.1 aDoran, Peter, T.1 aMyers, Madeline uhttps://www.cambridge.org/core/journals/antarctic-science/article/gis-tool-to-predict-photosynthetically-active-radiation-in-a-dry-valley/BD0BE4FF6A8F3DAAF32D69879728707803683nas a2200997 4500008004100000245008200041210006900123260001200192490000600204520084100210100002601051700001901077700002001096700001401116700002501130700002501155700001701180700001701197700001901214700002601233700002301259700002701282700002301309700002101332700003101353700001801384700002101402700002001423700003001443700001501473700002001488700001901508700002501527700002301552700001901575700002101594700001701615700002201632700002101654700002601675700002301701700001101724700001701735700002201752700002101774700003101795700002401826700002201850700001501872700002201887700001801909700001701927700002501944700002201969700001901991700002702010700001602037700001802053700002202071700003202093700002302125700002302148700002002171700002202191700002002213700002702233700001802260700002102278700001902299700002302318700001502341700002302356700002202379700002402401700002102425700002302446700002102469700001802490700001802508700001902526700001402545700002802559700001702587700002702604856005402631 2020 eng d00aA global database of soil nematode abundance and functional group composition0 aglobal database of soil nematode abundance and functional group  c03/20200 v73 a

As the most abundant animals on earth, nematodes are a dominant component of the soil community. they play critical roles in regulating biogeochemical cycles and vegetation dynamics within and across landscapes and are an indicator of soil biological activity. Here, we present a comprehensive global dataset of soil nematode abundance and functional group composition. This dataset includes 6,825 georeferenced soil samples from all continents and biomes. For geospatial mapping purposes these samples are aggregated into 1,933 unique 1-km pixels, each of which is linked to 73 global environmental covariate data layers. Altogether, this dataset can help to gain insight into the spatial distribution patterns of soil nematode abundance and community composition, and the environmental drivers shaping these patterns.

1 avan den Hoogen, Johan1 aGeisen, Stefan1 aWall, Diana, H.1 aWardle, D1 aTraunspurger, Walter1 ade Goede, Ron, G. M.1 aAdams, Byron1 aAhmad, Wasim1 aFerris, Howard1 aBardgett, Richard, D.1 aBonkowski, Michael1 aCampos-Herrera, Raquel1 aCares, Juvenil, E.1 aCaruso, Tancredi1 aCaixeta, Larissa, de Brito1 aChen, Xiaoyun1 aCosta, Sofia, R.1 aCreamer, Rachel1 aCastro, José, da Cunha e1 aDam, Marie1 aDjigal, Djibril1 aEscuer, Miguel1 aGriffiths, Bryan, S.1 aGutiérrez, Carmen1 aHohberg, Karin1 aKalinkina, Daria1 aKardol, Paul1 aKergunteuil, Alan1 aKorthals, Gerard1 aKrashevska, Valentyna1 aKudrin, Alexey, A.1 aLi, Qi1 aLiang, Wenju1 aMagilton, Matthew1 aMarais, Mariette1 aMartín, José, Antonio Ro1 aMatveeva, Elizaveta1 aMayad, El, Hassan1 aMzough, E.1 aMulder, Christian1 aMullin, Peter1 aNeilson, Roy1 aNguyen, T., A. Duong1 aNielsen, Uffe, N.1 aOkada, Hiroaki1 aRius, Juan, Emilio Pal1 aPan, Kaiwen1 aPeneva, Vlada1 aPellissier, Loïc1 ada Silva, Julio, Carlos Per1 aPitteloud, Camille1 aPowers, Thomas, O.1 aPowers, Kirsten1 aQuist, Casper, W.1 aRasmann, Sergio1 aMoreno, Sara, Sánchez1 aScheu, Stefan1 aSetälä, Heikki1 aSushchuk, Anna1 aTiunov, Alexei, V.1 aTrap, Jean1 aVestergård, Mette1 aVillenave, Cecile1 aWaeyenberge, Lieven1 aWilschut, Rutger1 aWright, Daniel, G.1 aKeith, Aidan, M.1 aYang, Jiue-in1 aSchmidt, Olaf1 aBouharroud, R.1 aFerji, Z.1 avan der Putten, Wim, H.1 aRouth, Devin1 aCrowther, Thomas, Ward uhttps://www.nature.com/articles/s41597-020-0437-302015nas a2200217 4500008004100000245016100041210006900202260001200271490000700283520122000290653001501510653001801525653002301543653003901566653001601605653003001621100002301651700002901674700002401703856007001727 2020 eng d00aGlycerol is an osmoprotectant in two Antarctic Chlamydomonas species from an ice-covered saline lake and is synthesized by an unusual bidomain enzyme0 aGlycerol is an osmoprotectant in two Antarctic IChlamydomonasI s c08/20200 v113 a

Glycerol, a compatible solute, has previously been found to act as an osmoprotectant in some marine Chlamydomonas species and several species of Dunaliella from hypersaline ponds. Recently, Chlamydomonas reinhardtii and Dunaliella salina were shown to make glycerol with an unusual bidomain enzyme, which appears to be unique to algae, that contains a phosphoserine phosphatase and glycerol-3-phosphate dehydrogenase. Here we report that two psychrophilic species of Chlamydomonas (C. spp. UWO241 and ICE-MDV) from Lake Bonney, Antarctica also produce high levels of glycerol to survive in the lake’s saline waters. Glycerol concentration increased linearly with salinity and at 1.3 M NaCl, exceeded 400 mM in C. sp. UWO241, the more salt-tolerant strain. We also show that both species expressed several isoforms of the bidomain enzyme. An analysis of one of the isoforms of C. sp. UWO241 showed that it was strongly upregulated by NaCl and is thus the likely source of glycerol. These results reveal another adaptation of the Lake Bonney Chlamydomonas species that allow them to survive in an extreme polar environment.

10aAntarctica10aChlamydomonas10aglycerol synthesis10aglycerol-3-phosphate dehydrogenase10aLake Bonney10aphosphoserine phosphatase1 aRaymond, James, A.1 aMorgan-Kiss, Rachael, M.1 aStahl-Rommel, Sarah uhttps://www.frontiersin.org/articles/10.3389/fpls.2020.01259/full02448nas a2200217 4500008004100000245010000041210006900141260001200210490000700222520174400229100001201973700001901985700002702004700002102031700001602052700001702068700002902085700002302114700002102137856007202158 2020 eng d00aMethane production in the oxygenated water column of a perennially ice‐covered Antarctic lake0 aMethane production in the oxygenated water column of a perennial c01/20200 v653 a

Aerobic methane production in aquatic ecosystems impacts the global atmospheric budget of methane, but the extent, mechanism, and taxa responsible for producing this greenhouse gas are not fully understood. Lake Bonney (LB), a perennially ice‐covered Antarctic lake, has cold hypersaline waters underlying an oxygenated freshwater layer. We present temporal methane concentration profiles in LB indicating methane production in the oxygenated (> 200% air saturation) water. Experiments amended with methylphosphonate (MPn) yielded methane generation, suggesting in situ methanogenesis via the carbon‐phosphorus (C‐P) lyase pathway. Enrichment cultures from the lake were used to isolate five bacterial strains capable of generating methane when supplied with MPn as the sole P source. Based on 16S rRNA gene sequencing, the isolates belong to the Proteobacteria (closely related to Marinomonas, Hoeflea, and Marinobacter genera) and Bacteroidetes (Algoriphagus genus). 16S rRNA metagenomic sequencing confirms the presence of these taxa in LB. None of the isolated species were reported to be capable to produce methane. In addition, orthologs of the phosphoenolpyruvate mutase gene (PepM) and methylphosphonate synthase (MPnS), enzymes involved in phosphonate and MPn biosynthesis, were widely spread in the LB shotgun metagenomic libraries; genes related to C‐P lyase pathways (phn gene clusters) were also abundant. 16S rRNA and mcrA genes of anaerobic methanogens were absent in both 16S rRNA and metagenomics libraries. These data reveal that in situ aerobic biological methane production is likely a significant source of methane in LB.

1 aLi, Wei1 aDore, John, E.1 aSteigmeyer, August, J.1 aCho, Yong‐Joon1 aKim, Ok-Sun1 aLiu, Yongqin1 aMorgan-Kiss, Rachael, M.1 aSkidmore, Mark, L.1 aPriscu, John, C. uhttps://aslopubs.onlinelibrary.wiley.com/doi/full/10.1002/lno.1125702051nas a2200253 4500008004100000245010800041210006900149260001200218520110900230653001201339653001401351653002401365653001701389653001901406653001701425100002401442700002501466700002201491700002001513700002101533700002101554700001601575856020601591 2020 eng d00aRemote characterization of photosynthetic communities in the Fryxell basin of Taylor Valley, Antarctica0 aRemote characterization of photosynthetic communities in the Fry c03/20203 a

We investigate the spatial distribution, spectral properties and temporal variability of primary producers (e.g. communities of microbial mats and mosses) throughout the Fryxell basin of Taylor Valley, Antarctica, using high-resolution multispectral remote-sensing data. Our results suggest that photosynthetic communities can be readily detected throughout the Fryxell basin based on their unique near-infrared spectral signatures. Observed intra- and inter-annual variability in spectral signatures are consistent with short-term variations in mat distribution, hydration and photosynthetic activity. Spectral unmixing is also implemented in order to estimate mat abundance, with the most densely vegetated regions observed from orbit correlating spatially with some of the most productive regions of the Fryxell basin. Our work establishes remote sensing as a valuable tool in the study of these ecological communities in the McMurdo Dry Valleys and demonstrates how future scientific investigations and the management of specially protected areas could benefit from these tools and techniques.

10aecology10ahydrology10aMcMurdo Dry Valleys10amicrobiology10aremote sensing10aspectroscopy1 aSalvatore, Mark, R.1 aBorges, Schuyler, R.1 aBarrett, John, E.1 aSokol, Eric, R.1 aStanish, Lee, F.1 aPower, Sarah, N.1 aMorin, Paul uhttps://www.cambridge.org/core/journals/antarctic-science/article/remote-characterization-of-photosynthetic-communities-in-the-fryxell-basin-of-taylor-valley-antarctica/8576F6BB1BCFDCA8409F5EA96CA00C6F01902nas a2200181 4500008004100000245011100041210006900152260003900221490000900260520124000269653001901509653002401528653002601552653001801578100002601596700002901622856006901651 2020 eng d00aResponse of microbial communities to climatic disturbances in Lake Bonney, McMurdo Dry Valleys, Antarctica0 aResponse of microbial communities to climatic disturbances in La aOxford, OHbMiami Universityc20200 vM.S.3 a

The McMurdo Dry Valleys is a polar desert ecosystem which composes the largest ice-free area in Antarctica, with the exception of perennially ice-covered lakes and ponds. The lakes in the valleys are the only landscape unit that support metabolic activity year-round. Recent increases in air temperature and solar radiation have led to a chain of disturbances altering the environmental conditions of these lakes. In this study, we test the impact of climatic disturbances on microbial communities in Lake Bonney, one of the lakes in the MDV. Through an integrated approach of combining field studies on natural communities in the lake (in situ) and laboratory experiments on algal isolates (ex situ), this study will attempt to understand how phytoplankton, eukaryal and bacterial communities respond to simulated disturbances. Results from the in situ experiments showed that the moat is a unique and stressful environment for under-ice communities and that under-ice shallow communities are highly sensitive to climatic disturbances. The ex situ experiments showed that certain phytoplankton species, like the chlorophytes, are more resistant to environmental alterations and thus will outcompete other phytoplankton species.

10aclimate change10aMcMurdo Dry Valleys10amicrobial communities10aphytoplankton1 aSherwell, Shasten, S.1 aMorgan-Kiss, Rachael, M. uhttp://rave.ohiolink.edu/etdc/view?acc_num=miami159595868836487703158nas a2200181 4500008004100000245011200041210006900153260001200222300001200234490000700246520256300253100002402816700002502840700002002865700002102885700002202906856004802928 2020 eng d00aThe seasonal evolution of albedo across glaciers and the surrounding landscape of Taylor Valley, Antarctica0 aseasonal evolution of albedo across glaciers and the surrounding c03/2020 a769-7880 v143 a

The McMurdo Dry Valleys (MDVs) of Antarctica are a polar desert ecosystem consisting of alpine glaciers, ice-covered lakes, streams, and expanses of vegetation-free rocky soil. Because average summer temperatures are close to 0 ∘C, the MDV ecosystem in general, and glacier melt dynamics in particular, are both closely linked to the energy balance. A slight increase in incoming radiation or change in albedo can have large effects on the timing and volume of meltwater. However, the seasonal evolution or spatial variability of albedo in the valleys has yet to fully characterized. In this study, we aim to understand the drivers of landscape albedo change within and across seasons. To do so, a box with a camera, GPS, and shortwave radiometer was hung from a helicopter that flew transects four to five times a season along Taylor Valley. Measurements were repeated over three seasons. These data were coupled with incoming radiation measured at six meteorological stations distributed along the valley to calculate the distribution of albedo across individual glaciers, lakes, and soil surfaces. We hypothesized that albedo would decrease throughout the austral summer with ablation of snow patches and increasing sediment exposure on the glacier and lake surfaces. However, small snow events (<6 mm water equivalent) coupled with ice whitening caused spatial and temporal variability of albedo across the entire landscape. Glaciers frequently followed a pattern of increasing albedo with increasing elevation, as well as increasing albedo moving from east to west laterally across the ablation zone. We suggest that spatial patterns of albedo are a function of landscape morphology trapping snow and sediment, longitudinal gradients in snowfall magnitude, and wind-driven snow redistribution from east to west along the valley. We also compare our albedo measurements to the MODIS albedo product and found that overall the data have reasonable agreement. The mismatch in spatial scale between these two datasets results in variability, which is reduced after a snow event due to albedo following valley-scale gradients of snowfall magnitude. These findings highlight the importance of understanding the spatial and temporal variability in albedo and the close coupling of climate and landscape response. This new understanding of landscape albedo can constrain landscape energy budgets, better predict meltwater generation on from MDV glaciers, and how these ecosystems will respond to changing climate at the landscape scale.

1 aBergstrom, Anna, J.1 aGooseff, Michael, N.1 aMyers, Madeline1 aDoran, Peter, T.1 aCross, Julian, M. uhttps://www.the-cryosphere.net/14/769/2020/02966nas a2200205 4500008004100000245011200041210006900153260001200222490000600234520228000240100002102520700002202541700002302563700002602586700002102612700001502633700002402648700001702672856007102689 2020 eng d00aSilicon isotopes reveal a non-glacial source of silicon to Crescent Stream, McMurdo Dry Valleys, Antarctica0 aSilicon isotopes reveal a nonglacial source of silicon to Cresce c06/20200 v83 a

In high latitude environments, silicon is supplied to river waters by both glacial and non-glacial chemical weathering. The signal of these two end-members is often obscured by biological uptake and/or groundwater input in the river catchment. McMurdo Dry Valleys streams in Antarctica have no deep groundwater input, no connectivity between streams and no surface vegetation cover, and thus provide a simplified system for us to constrain the supply of dissolved silicon (DSi) to rivers from chemical weathering in a glacial environment. Here we report dissolved Si concentrations, germanium/silicon ratios (Ge/Si) and silicon isotope compositions (δ30SiDSi) in Crescent Stream, McMurdo Dry Valleys for samples collected between December and February in the 2014−2015, 2015−2016, and 2016−2017 austral seasons. The δ30SiDSi compositions and DSi concentrations are higher than values reported in wet-based glacial meltwaters, and form a narrow cluster within the range of values reported for permafrost dominated Arctic Rivers. High δ30SiDSi compositions, ranging from +0.90‰ to +1.39‰, are attributed to (i) the precipitation of amorphous silica during freezing of waters in isolated pockets of the hyporheic zone in the winter and the release of Si from unfrozen pockets during meltwater-hyporheic zone exchange in the austral summer, and (ii) additional Si isotope fractionation via long-term Si uptake in clay minerals and seasonal Si uptake into diatoms superimposed on this winter-derived isotope signal. There is no relationship between δ30SiDSi compositions and DSi concentrations with seasonal and daily discharge, showing that stream waters contain DSi that is in equilibrium with the formation of secondary Si minerals in the hyporheic zone. We show that δ30SiDSi compositions can be used as tracers of silicate weathering in the hyporheic zone and possible tracers of freeze-thaw conditions in the hyporheic zone. This is important in the context of the ongoing warming in McMurdo Dry Valleys and the supply of more meltwaters to the hyporheic zone of McMurdo Dry Valley streams.

1 aHirst, Catherine1 aOpfergelt, Sophie1 aGaspard, François1 aHendry, Katharine, R.1 aHatton, Jade, E.1 aWelch, Sue1 aMcKnight, Diane, M.1 aLyons, Berry uhttps://www.frontiersin.org/articles/10.3389/feart.2020.00229/full02674nas a2200253 4500008004100000245007700041210006900118260001200187490000600199520187800205100002102083700002602104700002102130700002202151700001902173700002502192700002102217700002202238700001702260700002302277700002502300700002402325856007102349 2020 eng d00aSilicon isotopic composition of dry and wet-based glaciers in Antarctica0 aSilicon isotopic composition of dry and wetbased glaciers in Ant c07/20200 v83 a

Glaciers and ice sheets export significant amounts of silicon (Si) to downstream ecosystems, impacting local and potentially global biogeochemical cycles. Recent studies have shown Si in Arctic glacial meltwaters to have an isotopically distinct signature when compared to non-glacial rivers. This is likely linked to subglacial weathering processes and mechanochemical reactions. However, there are currently no silicon isotope (δ30Si) data available from meltwater streams in Antarctica, limiting the current inferences on global glacial silicon isotopic composition and its drivers. To address this gap, we present dissolved silicon (DSi), δ30SiDSi, and major ion data from meltwater streams draining a polythermal glacier in the region of the West Antarctic Peninsula (WAP; King George Island) and a cold-based glacier in East Antarctica [Commonwealth Stream, McMurdo Dry Valleys (MDV)]. These data, alongside other global datasets, improve our understanding of how contrasting glacier thermal regime can impact upon Si cycling and therefore the δ30SiDSi composition. We find a similar δ30SiDSi composition between the two sites, with the streams on King George Island varying between -0.23 and +1.23‰ and the Commonwealth stream varying from -0.40 to +1.14‰. However, meltwater streams in King George Island have higher DSi concentrations, and the two glacial systems exhibit opposite DSi – δ30SiDSi trends. These contrasts likely result from differences in weathering processes, specifically the role of subglacial processes (King George Island) and, supraglacial processes followed by in-stream weathering in hyporheic zones (Commonwealth Stream). These findings are important when considering likely changes in nutrient fluxes from Antarctic glaciers under climatic warming scenarios and consequent shifts in glacial thermal regimes.

1 aHatton, Jade, E.1 aHendry, Katharine, R.1 aHirst, Catherine1 aOpfergelt, Sophie1 aHenkel, Susann1 aSilva-Busso, Adrián1 aWelch, Susan, A.1 aWadham, Jemma, L.1 aLyons, Berry1 aBagshaw, Elizabeth1 aStaubwasser, Michael1 aMcKnight, Diane, M. uhttps://www.frontiersin.org/articles/10.3389/feart.2020.00286/full02357nas a2200205 4500008004100000245012200041210006900163260001200232490000700244520163100251100002501882700002701907700001801934700002501952700002501977700002902002700002502031700002302056856007202079 2020 eng d00aVertical stratification and stability of biogeochemical processes in the deep saline waters of Lake Vanda, Antarctica0 aVertical stratification and stability of biogeochemical processe c03/20200 v653 a

Lake Vanda is a permanently ice-covered lake in the McMurdo Dry Valleys of Antarctica. Its bottom waters remain stratified year-round because of a strong salinity-driven density gradient. We have assessed spatial patterns in and relationships between major biogeochemical processes in the water column of Lake Vanda. Samples were collected in the austral summers of 2008 and 2011 to measure concentrations of metabolites associated with a suite of biogeochemical processes across the deep salinity gradient. The shapes of the resulting geochemical profiles were consistent between 2008 and 2011. Metabolite production and consumption rates were estimated using a reactive transport model based on the assumption that vertical diffusion was the only activephysical transport process. We validated this model for nitrification by using stable isotope incubations to show that this process was only active at depths predicted by the model. No nitrification activity was observed at 68 m depth in spite of overlapping oxygen and ammonium gradients. We attribute this lack of activity to the competitive inhibition of ammonia monooxygenase by methane. Net nitrous oxide and nitrate consumption were observed in the oxic water column, providing evidence of aerobic denitrification. The depth of maximum net oxygen production did not coincide with the deep chlorophyll maxima (at 59.3, 63, and 68.2 m) measured in the same profile. Finally, the integrated sulfide oxidation rate was high compared with other oxidation processes, indicating that sulfide was an important electron donor for the water column microbial community.

1 aSchutte, Charles, A.1 aSamarkin, Vladimir, A.1 aPeters, Brian1 aMadigan, Michael, T.1 aBowles, Marshall, W.1 aMorgan-Kiss, Rachael, M.1 aCasciotti, Karen, L.1 aJoye, Samantha, B. uhttps://aslopubs.onlinelibrary.wiley.com/doi/full/10.1002/lno.1132702684nas a2200265 4500008004100000022001400041245014900055210006900204260001200273490000600285520185300291653001502144653002502159653000902184653001802193653001702211100001502228700001802243700001602261700001202277700001402289700002102303700002902324856006502353 2019 eng d a0166-859500aThe Antarctic psychrophiles Chlamydomonas spp. UWO241 and ICE-MDV exhibit differential restructuring of photosystem I in response to iron0 aAntarctic psychrophiles iChlamydomonasi spp UWO241 and ICEMDV ex c02/20190 v93 a

Chlamydomonas sp. UWO241 is a psychrophilic alga isolated from the deep photic zone of a perennially ice-covered Antarctic lake (east lobe Lake Bonney, ELB). Past studies have shown that C. sp. UWO241 exhibits constitutive downregulation of photosystem I (PSI) and high rates of PSI-associated cyclic electron flow (CEF). Iron levels in ELB are in the nanomolar range leading us to hypothesize that the unusual PSI phenotype of C. sp. UWO241 could be a response to chronic Fe-deficiency. We studied the impact of Fe availability in C. sp. UWO241, a mesophile, C. reinhardtii SAG11-32c, as well as a psychrophile isolated from the shallow photic zone of ELB, Chlamydomonas sp. ICE-MDV. Under Fe-deficiency, PsaA abundance and levels of photooxidizable P700 (ΔA820/A820) were reduced in both psychrophiles relative to the mesophile. Upon increasing Fe, C. sp. ICE-MDV and C. reinhardtii exhibited restoration of PSI function, while C. sp. UWO241 exhibited only moderate changes in PSI activity and lacked almost all LHCI proteins. Relative to Fe-excess conditions (200 μM Fe2+), C. sp. UWO241 grown in 18 μM Fe2+ exhibited downregulation of light harvesting and photosystem core proteins, as well as upregulation of a bestrophin-like anion channel protein and two CEF-associated proteins (NdsS, PGL1). Key enzymes of starch synthesis and shikimate biosynthesis were also upregulated. We conclude that in response to variable Fe availability, the psychrophile C. sp. UWO241 exhibits physiological plasticity which includes restructuring of the photo-chemical apparatus, increased PSI-associated CEF, and shifts in downstream carbon metabolism toward storage carbon and secondary stress metabolites.

10aAntarctica10aCyclic electron flow10aIron10aPhotosystem I10aPsychrophile1 aCook, Greg1 aTeufel, Amber1 aKalra, Isha1 aLi, Wei1 aWang, Xin1 aPriscu, John, C.1 aMorgan-Kiss, Rachael, M. uhttps://link.springer.com/article/10.1007/s11120-019-00621-002353nas a2200397 4500008004100000245012200041210006900163260001200232490000600244520115100250100002101401700002501422700002101447700002101468700001401489700002201503700001701525700002201542700001701564700002001581700002001601700002401621700002201645700001901667700002401686700002401710700001701734700002301751700002001774700001801794700002401812700002201836700002401858700001901882856005401901 2019 eng d00aBiotic interactions are an unexpected yet critical control on the complexity of an abiotically driven polar ecosystem0 aBiotic interactions are an unexpected yet critical control on th c02/20190 v23 a

Abiotic and biotic factors control ecosystem biodiversity, but their relative contributions remain unclear. The ultraoligotrophic ecosystem of the Antarctic Dry Valleys, a simple yet highly heterogeneous ecosystem, is a natural laboratory well-suited for resolving the abiotic and biotic controls of community structure. We undertook a multidisciplinary investigation to capture ecologically relevant biotic and abiotic attributes of more than 500 sites in the Dry Valleys, encompassing observed landscape heterogeneities across more than 200 km2. Using richness of autotrophic and heterotrophic taxa as a proxy for functional complexity, we linked measured variables in a parsimonious yet comprehensive structural equation model that explained significant variations in biological complexity and identified landscape-scale and fine-scale abiotic factors as the primary drivers of diversity. However, the inclusion of linkages among functional groups was essential for constructing the best-fitting model. Our findings support the notion that biotic interactions make crucial contributions even in an extremely simple ecosystem.

1 aLee, Charles, K.1 aLaughlin, Daniel, C.1 aBottos, Eric, M.1 aCaruso, Tancredi1 aJoy, Kurt1 aBarrett, John, E.1 aBrabyn, Lars1 aNielsen, Uffe, N.1 aAdams, Byron1 aWall, Diana, H.1 aHopkins, D., W.1 aPointing, Steve, B.1 aMcDonald, Ian, R.1 aCowan, Don, A.1 aBanks, Jonathan, C.1 aStichbury, Glen, A.1 aJones, Irfon1 aZawar-Reza, Peyman1 aKaturji, Marwan1 aHogg, Ian, D.1 aSparrow, Ashley, D.1 aStorey, Bryan, C.1 aGreen, T., G. Allan1 aCary, Craig, S uhttps://www.nature.com/articles/s42003-018-0274-502977nas a2200121 4500008004100000245014300041210006900184260005700253490001000310520246100320100002202781856005202803 2019 eng d00aCharacterizing photobioregenerative technology for simulataneous thermal control and air revitalization of spacecraft and surface habitats0 aCharacterizing photobioregenerative technology for simulataneous aBoulder, CObUniversity of Colorado Boulderc11/20190 vPh.D.3 a

Algal photobioreactors have been researched as potential solutions to air revitalization in a spacecraft cabin environment by absorbing CO2 and producing O2 through photosynthesis. This photosynthesis, and consumption of produced biomass, theoretically provides a closed-loop solution for long-duration spaceflight. Addressing multiple spaceflight requirements simultaneously with algae has the potential to reduce launch mass, power and volume of future Environmental Control and Life Support (ECLS) systems. Additionally, inoculating algal culture into a water-based thermal cooling loops (flight-proven standard of active cooling found on the International Space Station (ISS)) could incorporate simultaneous air revitalization and thermal control into a common system. However, this imparts rapid, extreme thermal swings on algal cells not evolved for culture in a transient thermal environment. Therefore, the effect of dynamic thermal environments on the CO2/O2 turnover of algae was characterized to provide a first-order assessment of system feasibility. This research characterizes the effect of dynamic environments, both transient thermal environments and varying levels of CO2 concentration, on metabolic processes of the algal culture. Experiments using Antarctic algal species were included to investigate if cold-acclimated algae are more efficient than Chlorella at CO2/O2 turnover in the active cooling environment. The simultaneous heat and mass transfer coefficients of a nonporous, gas-permeable membrane were characterized, and membrane models developed for future design considerations. A photobioreactor system was designed with considerations for gravity-independence, prototyped, and tested using parameters defined by the ISS cabin environment. A failure modes and effect analysis distilled lessons learned from the previous experiments, which also informs the use of algae for bioregenerative life support. In conclusion, the resulting values from the previous characterization experiments, along with values found in literature, were used to make a first-order mass-balance comparison between current ISS ECLSS and photobioregenerative technologies. This work serves as an initial evaluation of the feasibility for using an algal photobioreactor for simultaneous air revitalization and active thermal control of a spacecraft or surface habitat.

1 aMatula, Emily, E. uhttps://scholar.colorado.edu/asen_gradetds/258/02939nas a2200241 4500008004100000245010800041210006900149260001200218300001100230490000800241520220100249653000902450653001602459653003202475653001302507653001302520100001602533700001802549700001402567700001502581700002902596856007202625 2019 eng d00aCommunity response of microbial primary producers to salinity is primarily driven by nutrients in lakes0 aCommunity response of microbial primary producers to salinity is c12/2019 a1340010 v6963 a

Higher microbial diversity was frequently observed in saline than fresh waters, but the underlying mechanisms remains unknown, particularly in microbial primary producers (MPP). MPP abundance and activity are notably constrained by high salinity, but facilitated by high nutrients. It remains to be ascertained whether and how nutrients regulate the salinity constraints on MPP abundance and community structure. Here we investigated the impact of nutrients on salinity constraints on MPP abundance and diversity in undisturbed lakes with a wide salinity range on the Tibetan Plateau. MPP community was explored using quantitative PCR, terminal restriction fragment length polymorphism and sequencing of cloning libraries targeting form IC cbbL gene. The MPP community structure was sorted by salinity into freshwater (salinity<1‰), saline (1‰ < salinity<29‰) and hypersaline (salinity>29‰) lakes. Furthermore, while MPP abundance, diversity and richness were significantly constrained with increasing salinity, these constraints were mitigated by enhancing total organic carbon (TOC) and total nitrogen (TN) contents in freshwater and saline lakes. In contrast, the MPP diversity increased significantly with the salinity in hypersaline lakes, due to the mitigation of enhancing TOC and TN contents and salt-tolerant MPP taxa. The mitigating effect of nutrients was more pronounced in saline than in freshwater and hypersaline lakes. The MPP compositions varied along salinity, with Betaproteobacteria dominating both the freshwater and saline lakes and Gammaproteobacteria dominating the hypersaline lakes. We concluded that high nutrients could mitigate the salinity constraining effects on MPP abundance, community richness and diversity. Our findings offer a novel insight into the salinity effects on primary producers and highlight the interactive effects of salinity and nutrients on MPP in lakes. These findings can be used as a baseline to illuminate the effects of increased anthropogenic activities altering nutrient dynamics on the global hydrological cycle and the subsequent responses thereof by MPP communities.

10acbbL10alake waters10amicrobial primary producers10anutrient10asalinity1 aYue, Linyan1 aKong, Weidong1 aJi, Mukan1 aLiu, Jinbo1 aMorgan-Kiss, Rachael, M. uhttps://www.sciencedirect.com/science/article/pii/S004896971933978602367nas a2200205 4500008004100000245011400041210006900155260001200224300001400236490000800250520166200258100002501920700002701945700002801972700002602000700002002026700002002046700002102066856007402087 2019 eng d00aDifferential incorporation of bacteria, organic matter, and inorganic ions into lake ice during ice formation0 aDifferential incorporation of bacteria organic matter and inorga c02/2019 a585 - 6000 v1243 a

The segregation of bacteria, inorganic solutes, and total organic carbon between liquid water and ice during winter ice formation on lakes can significantly influence the concentration and survival of microorganisms in icy systems, and their roles in biogeochemical processes. Our study quantifies the distributions of bacteria and solutes between liquid and solid water phases during progressive freezing. We simulated lake ice formation in mesocosm experiments using water from perennially (Antarctica) and seasonally (Alaska and Montana, USA) ice covered lakes. We then computed concentration factors and effective segregation coefficients, which are parameters describing the incorporation of bacteria and solutes into ice. Experimental results revealed that, contrary to major ions, bacteria were readily incorporated into ice and did not concentrate in the liquid phase. The organic matter incorporated into the ice was labile, amino acid-like material, differing from the humic-like compounds that remained in the liquid phase. Results from a control mesocosm experiment (dead bacterial cells) indicated that viability of bacterial cells did not influence the incorporation of free bacterial cells into ice, but did have a role in the formation and incorporation of bacterial aggregates. Together, these findings demonstrate that bacteria, unlike other solutes, were preferentially incorporated into lake-ice during our freezing experiments, a process controlled mainly by the initial solute concentration of the liquid water source, regardless of cell viability.

1 aSantibáñez, Pamela1 aMichaud, Alexander, B.1 aVick-Majors, Trista, J.1 aD’Andrilli, Juliana1 aChiuchiolo, Amy1 aHand, Kevin, P.1 aPriscu, John, C. uhttps://agupubs.onlinelibrary.wiley.com/doi/full/10.1029/2018JG00482501931nas a2200229 4500008004100000245010800041210006900149260001200218490000700230520115400237100002101391700001701412700001701429700002401446700002401470700001601494700001601510700001701526700001501543700002301558856012001581 2019 eng d00aDiurnal chemistry of two contrasting stream types, Taylor Valley, McMurdo Dry Valley Region, Antarctica0 aDiurnal chemistry of two contrasting stream types Taylor Valley  c06/20190 v983 a

Numerous ephemeral streams flow within the McMurdo Dry Valley Region of Antarctica that transport glacial meltwater to perennially ice-covered, closed-basin lakes during the austral summer. The diurnal behavior for two Taylor Valley streams of different character was examined during the summer of 2010-11. Andersen Creek is a short, 1st-order proglacial stream, whereas Von Guerard Stream is a long, high-order stream with an extensive hyporheic zone that has a substantial cyanobacterial algal mat community in its middle reaches. Both streams display strong daily cycles for temperature, electrical conductivity, dissolved oxygen, and pH. Conductivity varies in concert with flow, with solute dilution occurring during the daily high-flow pulse. Dissolved oxygen co-varies strongly with pH at Andersen Creek but not for Von Guerard Stream. Each stream has a distinct geochemical character that for Andersen Creek is a direct reflection of its glacial source, unmodified by secondary effects, whereas that for Von Guerard Stream is modulated by its resident algal mat community and through extensive hyporheic zone interaction and exchange.

1 aHarmon, Mark, E.1 aLeslie, D.L.1 aLyons, Berry1 aWelch, Kathleen, A.1 aMcKnight, Diane, M.1 aChudaev, O.1 aKharaka, Y.1 aHarmon, R.S.1 aMillot, R.1 aShouakar-Stash, O. uhttps://www.e3s-conferences.org/articles/e3sconf/abs/2019/24/e3sconf_wri-162018_01020/e3sconf_wri-162018_01020.html02573nas a2200265 4500008004100000022001400041245010400055210006900159260001200228300001400240490000700254520177600261653002002037653002102057653001502078653001702093653002402110653001202134653002502146100001202171700002002183700001602203700002902219856005902248 2019 eng d a0948-305500aDrivers of protistan community autotrophy and heterotrophy in chemically stratified Antarctic lakes0 aDrivers of protistan community autotrophy and heterotrophy in ch c01/2019 a225 - 2390 v823 a

Single-celled, eukaryotic microorganisms, known as protists, are responsible for 2 important, yet opposing, metabolic activities within aquatic food webs. They are major primary producers and highly active predators in marine and fresh water systems. While genomics has accelerated in recent years for this taxonomically diverse group, our understanding of the metabolic capabilities of most protists remains limited. It is also poorly understood how protist trophic mode is affected by biotic and abiotic factors, and therefore it is difficult to predict how events such as global climate change will affect the balance between autotrophic and heterotrophic activities in protist communities. To address open questions regarding how protist metabolic versatility is influenced by their environment, we characterized the potential for carbon fixation versus organic carbon degradation using enzymatic assays (RubisCO and β-D-glucosaminidase, respectively) within the water columns of ice-covered lakes in McMurdo Dry Valleys (MDV), Antarctica. Steep physical and chemical gradients in the water columns, microorganism domination and minimal allochthonous inputs makes the MDV lakes uniquely suited to investigate environment-microbe interactions. Spatial trends in RubisCO and β-D-glucosaminidase activities were lake-specific and vertically stratified within the water columns. Moreover, bottom-up drivers controlling the activity of C-fixation vs. organic C-degradation among the MDV protist communities were distinct between the upper photic vs. the deep, aphotic zones. We conclude that differential controls over major C-cycling enzymes have important implications on the influence of environmental change on the carbon and nutrient cycles in the MDV lakes.

10aAntarctic lakes10aAquatic protists10aAutotrophy10aHeterotrophy10aMcMurdo Dry Valleys10aRubisCO10aβ-D-glucosaminidase1 aLi, Wei1 aDolhi-Binder, J1 aCariani, ZE1 aMorgan-Kiss, Rachael, M. uhttps://www.int-res.com/abstracts/ame/v82/n3/p225-239/02611nas a2200253 4500008004100000245011600041210006900157260001200226520177500238653001202013653002502025653003202050653001402082653002402096100002102120700002202141700002802163700002102191700002302212700002102235700001502256700002302271856006302294 2019 eng d00aEnvironmental controls on bacteriohopanepolyol profiles of benthic microbial mats from Lake Fryxell, Antarctica0 aEnvironmental controls on bacteriohopanepolyol profiles of benth c07/20193 a

Bacteriohopanepolyols (BHPs) are pentacyclic triterpenoid lipids that contribute to the structural integrity and physiology of some bacteria. Because some BHPs originate from specific classes of bacteria, BHPs have potential as taxonomically and environmentally diagnostic biomarkers. For example, a stereoisomer of bacteriohopanetetrol (informally BHT II) has been associated with anaerobic ammonium oxidation (anammox) bacteria and suboxic to anoxic marine environments where anammox is active. As a result, the detection of BHT II in the sedimentary record and fluctuations in the relative abundance of BHT II may inform reconstructions of nitrogen cycling and ocean redox changes through the geological record. However, there are uncertainties concerning the sources of BHT II and whether or not BHT II is produced in abundance in non‐marine environments, both of which are pertinent to interpretations of BHT II signatures in sediments. To address these questions, we investigate the BHP composition of benthic microbial mats from Lake Fryxell, Antarctica. Lake Fryxell is a perennially ice‐covered lake with a sharp oxycline in a density‐stabilized water column. We describe the diversity and abundance of BHPs in benthic microbial mats across a transect from oxic to anoxic conditions. Generally, BHP abundances and diversity vary with the morphologies of microbial mats, which were previously shown to reflect local environmental conditions, such as irradiance and oxygen and sulfide concentrations. BHT II was identified in mats that exist within oxic to anoxic portions of the lake. However, anammox bacteria have yet to be identified in Lake Fryxell. We examine our results in the context of BHPs as biomarkers in modern and ancient environments.

10aanammox10abacteriohopanepolyol10abacteriohopanetetrol isomer10abiomarker10aMcMurdo Dry Valleys1 aMatys, Emily, D.1 aMackey, Tyler, J.1 aGrettenberger, Christen1 aMueller, Elliott1 aJungblut, Anne, D.1 aSumner, Dawn, Y.1 aHawes, Ian1 aSummons, Roger, E. uhttps://onlinelibrary.wiley.com/doi/full/10.1111/gbi.1235302504nas a2200229 4500008004100000245007200041210006700113260001200180490000800192520178600200100001701986700002202003700002202025700002402047700001502071700002902086700002502115700002102140700002002161700002002181856007302201 2019 eng d00aThe Geochemistry of Englacial Brine From Taylor Glacier, Antarctica0 aGeochemistry of Englacial Brine From Taylor Glacier Antarctica c03/20190 v1243 a

Blood Falls is a hypersaline, iron‐rich discharge at the terminus of the Taylor Glacier in the McMurdo Dry Valleys, Antarctica. In November 2014, brine in a conduit within the glacier was penetrated and sampled using clean‐entry techniques and a thermoelectric melting probe called the IceMole. We analyzed the englacial brine sample for filterable iron (fFe), total Fe, major cations and anions, nutrients, organic carbon, and perchlorate. In addition, aliquots were analyzed for minor and trace elements and isotopes including δD and δ18O of water, δ34S and δ18O of sulfate, 234U, 238U, δ11B, 87Sr/86Sr, and δ81Br. These measurements were made in order to (1) determine the source and geochemical evolution of the brine and (2) compare the chemistry of the brine to that of nearby hypersaline lake waters and previous supraglacially sampled collections of Blood Falls outflow that were interpreted as end‐member brines. The englacial brine had higher Cl− concentrations than the Blood Falls end‐member outflow; however, other constituents were similar. The isotope data indicate that the water in the brine is derived from glacier melt. The H4SiO4 concentrations and U and Sr isotope suggest a high degree of chemical weathering products. The brine has a low N:P ratio of ~7.2 with most of the dissolved inorganic nitrogen in the form of NH4+. Dissolved organic carbon concentrations are similar to end‐member outflow values. Our results provide strong evidence that the original source of solutes in the brine was ancient seawater, which has been modified with the addition of chemical weathering products.

1 aLyons, Berry1 aMikucki, Jill, A.1 aGerman, Laura, A.1 aWelch, Kathleen, A.1 aWelch, Sue1 aGardner, Christopher, B.1 aTulaczyk, Slawek, M.1 aPettit, Erin, C.1 aKowalski, Julia1 aDachwald, Bernd uhttps://agupubs.onlinelibrary.wiley.com/doi/abs/10.1029/2018JG00441102639nas a2200301 4500008004100000245008400041210006900125260001200194520169800206653001501904653002101919653002401940653002701964653001301991100002102004700002602025700001702051700001602068700002502084700002502109700001702134700002202151700002302173700002002196700002402216700002402240856007302264 2019 eng d00aThe hydroecology of an ephemeral wetland in the McMurdo Dry Valleys, Antarctica0 ahydroecology of an ephemeral wetland in the McMurdo Dry Valleys  c11/20193 a

The McMurdo Dry Valleys (MDV) is a polar desert on the coast of East Antarctica where ephemeral wetlands become hydrologically active during warm and sunny summers when sub‐surface flows are generated from melting snowfields. To understand the structure and function of polar wetland ecosystems, we investigated the hydroecology of one such wetland, the Wormherder Creek wetland, during the warm and sunny summer of 2008 – 2009, when the wetland was hydrologically reactivated. Conservative tracer (LiCl) was injected for a 2‐hour period into a stream above the wetland to determine flow path orientations and hydrologic residence times. Tracer results indicated that surface water is rapidly exchanged with wetland groundwater and wetland residence times may exceed two austral summers. Major ion concentrations were uniform in samples from surface water and shallow groundwater throughout the wetland. Microbial mats in the wetland had high autotrophic index values (the ratios of chlorophyll a [Chl‐a]/ash‐free dry mass [AFDM]), ranging from 9‐38 μg Chl‐a/mg AFDM, indicative of actively photosynthesizing mat communities. The diatom communities in the mats were relatively uniform compared to those in mats from regularly flowing MDV streams, with four endemic and one widespread diatom taxa of the genus Luticola accounting for an average of 86% of the community. These results indicate that the hydrologic characteristics of the wetland contribute to uniform geochemical conditions. In turn, uniform geochemical conditions may explain the high autotrophic index values of the microbial mats and relatively low spatial variation of the diatom community.

10aAntarctica10adesert hydrology10adiatom biodiversity10ahyporheic interactions10awetlands1 aWlostowski, Adam1 aSchulte, Nicholas, O.1 aAdams, Byron1 aBall, Becky1 aEsposito, Rhea, M.M.1 aGooseff, Michael, N.1 aLyons, Berry1 aNielsen, Uffe, N.1 aVirginia, Ross, A.1 aWall, Diana, H.1 aWelch, Kathleen, A.1 aMcKnight, Diane, M. uhttps://agupubs.onlinelibrary.wiley.com/doi/abs/10.1029/2019JG00515302158nas a2200229 4500008004100000245010300041210006900144260003900213490000900252520137700261653001001638653002801648653003801676653002401714653001901738653001801757653002301775653001601798100001601814700002901830856006901859 2019 eng d00aImpact of simulated polar night on Antarctic mixotrophic and strict photoautotrophic phytoplankton0 aImpact of simulated polar night on Antarctic mixotrophic and str aOxford, OHbMiami Universityc20180 vM.S.3 a

Phytoplankton in polar regions experience long periods of continuous darkness annually during the polar night. Due to difficulties in performing field work during this period, it is largely unknown how phytoplankton endure this extreme transition from 24-hour daylight in the fall to several months of total darkness in the austral winter. The primary goal of this study was to compare physiological and photosynthetic responses of several Antarctic phytoplankton of variable trophic abilities (pure photosynthetic vs. mixotrophic) to simulated polar night conditions, including the transition seasons before and after winter. Two distinct responses were observed to extended darkness: (1) strict photoautotrophs (Chlamydomonas sp. ICE-MDV and Chlamydomonas sp. UWO241) exhibited functional downregulation their photosynthetic processes in the winter, followed by a lag phase of several days during mimicked spring, and (2) mixotrophs (Isochrysis sp. MDV and Geminigera cryophila) maintained functional photosynthetic apparatus, increased heterotrophy through the winter, and exhibited immediate growth upon return to light incubation. These differing responses to mimicked polar night conditions could represent two different strategies for surviving the long period of darkness in the phytoplankton’s natural environment.

10aalgae10aAntarctic phytoplankton10achlorophyll fluorescence analysis10aMcMurdo Dry Valleys10aPhotosynthesis10aphytoplankton10apolar microbiology10apolar night1 aCariani, ZE1 aMorgan-Kiss, Rachael, M. uhttp://rave.ohiolink.edu/etdc/view?acc_num=miami154720459996908102449nas a2200169 4500008004100000245008400041210006900125260001200194490000700206520189300213100003002106700002102136700002202157700001502179700002302194856006202217 2019 eng d00aIn a PICL: The sedimentary deposits and facies of perennially ice-covered lakes0 aIn a PICL The sedimentary deposits and facies of perennially ice c04/20190 v663 a

Perennially ice‐covered lakes can have significantly different facies than open‐water lakes because sediment is transported onto the ice, where it accumulates, and sand grains preferentially melt through to be deposited on the lake floor. To characterize the facies in these lakes, sedimentary deposits from five Antarctic perennially ice‐covered lakes were described using lake‐bottom observations, underwater video and images, and sediment cores. One lake was dominated by laminated microbial mats and mud (derived from an abutting glacier), with disseminated sand and rare gravel. The other four lakes were dominated by laminated microbial mats and moderately well to moderately sorted medium to very coarse sand with sparse granules and pebbles; they contained minor interstitial or laminated mud (derived from streams and abutting glaciers). The sand was disseminated or localized in mounds and 1 m to more than 10 m long elongate ridges. Mounds were centimetres to metres in diameter; conical, elongate or round in shape; and isolated or deposited near or on top of one another. Sand layers in the mounds had normal, inverse, or no grading. Nine mixed mud and sand facies were defined for perennially ice‐covered lakes based on the relative proportion of mud to sand and the style of sand deposition. While perennially ice‐covered lake facies overlap with other ice‐influenced lakes and glaciomarine facies, they are characterized by a paucity of grains coarser than granules, a narrow range in sand grain sizes, and inverse grading in the sand mounds. These facies can be used to infer changes in ice cover through time and to identify perennially ice‐covered lakes in the rock record. Ancient perennially ice‐covered lakes are expected on Earth and Mars, and their characterization will provide new insights into past climatic conditions and habitability.

1 aRivera-Hernandez, Frances1 aSumner, Dawn, Y.1 aMackey, Tyler, J.1 aHawes, Ian1 aAndersen, Dale, T. uhttps://onlinelibrary.wiley.com/doi/abs/10.1111/sed.1252202372nas a2200193 4500008004100000245016100041210006900202260001200271490000700283520165600290653002101946653002601967653002701993653001702020653002902037100001202066700002902078856007102107 2019 eng d00aInfluence of environmental drivers and potential interactions on the distribution of microbial communities from three permanently stratified Antarctic lakes0 aInfluence of environmental drivers and potential interactions on c05/20190 v103 a

The McMurdo Dry Valley (MDV) lakes represent unique habitats in the microbial world. Perennial ice covers protect liquid water columns from either significant allochthonous inputs or seasonal mixing, resulting in centuries of stable biogeochemistry. Extreme environmental conditions including low seasonal photosynthetically active radiation (PAR), near freezing temperatures, and oligotrophy have precluded higher trophic levels from the food webs. Despite these limitations, diverse microbial life flourishes in the stratified water columns, including Archaea, bacteria, fungi, protists, and viruses. While a few recent studies have applied next generation sequencing, a thorough understanding of the MDV lake microbial diversity and community structure is currently lacking. Here we used Illumina MiSeq sequencing of the 16S and 18S rRNA genes combined with a microscopic survey of key eukaryotes to compare the community structure and potential interactions among the bacterial and eukaryal communities within the water columns of Lakes Bonney (east and west lobes, ELB, and WLB, respectively) and Fryxell (FRX). Communities were distinct between the upper, oxic layers and the dark, anoxic waters, particularly among the bacterial communities residing in WLB and FRX. Both eukaryal and bacterial community structure was influenced by different biogeochemical parameters in the oxic and anoxic zones. Bacteria formed complex interaction networks which were lake-specific. Several eukaryotes exhibit potential interactions with bacteria in ELB and WLB, while interactions between these groups in the more productive FRX were relatively rare.

10aAquatic protists10aenvironmental drivers10aheterotrophic bacteria10ainteractions10aMcMurdo Dry Valley lakes1 aLi, Wei1 aMorgan-Kiss, Rachael, M. uhttps://www.frontiersin.org/articles/10.3389/fmicb.2019.01067/full02300nas a2200289 4500008004100000245009400041210006900135260001200204490000800216520140200224653002001626653001501646653002001661653002401681653001401705653001901719100001901738700002501757700002401782700002601806700002201832700001901854700002501873700002401898700002401922856006401946 2019 eng d00aSabbea gen. nov., a new diatom genus (Bacillariophyta) from continental Antarctica0 aiSabbea gen novi a new diatom genus Bacillariophyta from contine c09/20190 v4183 a

The non-marine diatom flora of the Antarctic Continent includes several endemic taxa recorded over the past 100 years. One of these taxa, Navicula adminensis D.Roberts & McMinn, was described from the Vestfold Hills, East Antarctica. Detailed light and scanning electron microscopy observations have shown that based on its morphological features, the species does not belong to the genus Navicula sensu stricto. To determine the most closely related genera to N. adminensis, the morpho- logical features of Adlafia, Kobayasiella, Envekadea, Stenoneis, Berkeleya, Climaconeis, and Parlibellus were compared with those of N. adminensis. Although each of these genera shows one or more similar features, none of them accommodates the salient morphological characteristics of N. adminensis. Therefore, a new genus, Sabbea gen. nov., is herein described, and Navicula adminensis is formally transferred to the new genus as Sabbea adminensis comb. nov. The genus Sabbea is characterized by uniseriate striae composed of small, rounded areolae occluded externally by individual hymenes, a rather simple raphe structure with straight, short proximal ends and short terminal raphe fissures, open girdle bands with double perforation and a very shallow mantle.

10aBacillariophyta10aCape Royds10aEast Antarctica10aMcMurdo Dry Valleys10anew genus10aVestfold Hills1 aBishop, Jordan1 aKopalová, Kateřina1 aDarling, Joshua, P.1 aSchulte, Nicholas, O.1 aKohler, Tyler, J.1 aMcMinn, Andrew1 aSpaulding, Sarah, A.1 aMcKnight, Diane, M.1 aVan de Vijver, Bart uhttps://www.mapress.com/j/pt/article/view/phytotaxa.418.1.201888nas a2200301 4500008004100000245004500041210004100086260001200127300001300139490000600152520103800158100001501196700002301211700002801234700002401262700002201286700002501308700001401333700002301347700002401370700002201394700002001416700002601436700001601462700002301478700001601501856006901517 2019 eng d00aThe polar regions in a 2°C warmer world0 apolar regions in a 2°C warmer world c12/2019 aeaaw98830 v53 a

Over the past decade, the Arctic has warmed by 0.75°C, far outpacing the global average, while Antarctic tem- peratures have remained comparatively stable. As Earth approaches 2°C warming, the Arctic and Antarctic may reach 4°C and 2°C mean annual warming, and 7°C and 3°C winter warming, respectively. Expected consequences of increased Arctic warming include ongoing loss of land and sea ice, threats to wildlife and traditional human livelihoods, increased methane emissions, and extreme weather at lower latitudes. With low biodiversity, Antarctic ecosystems may be vulnerable to state shifts and species invasions. Land ice loss in both regions will contribute substantially to global sea level rise, with up to 3 m rise possible if certain thresholds are crossed. Mitigation efforts can slow or reduce warming, but without them northern high latitude warming may accelerate in the next two to four decades. International cooperation will be crucial to foreseeing and adapting to expected changes.

1 aPost, Eric1 aAlley, Richard, B.1 aChristensen, Torben, R.1 aMacias-Fauria, Marc1 aForbes, Bruce, C.1 aGooseff, Michael, N.1 aIler, Amy1 aKerby, Jeffrey, T.1 aLaidre, Kristin, L.1 aMann, Michael, E.1 aOlofsson, Johan1 aStroeve, Julienne, C.1 aUlmer, Fran1 aVirginia, Ross, A.1 aWang, Muyin uhttp://advances.sciencemag.org/lookup/doi/10.1126/sciadv.aaw988303892nas a2200949 4500008004100000245007900041210006900120260001200189490000800201520116600209100002601375700001901401700001701420700001901437700002501456700001401481700002501495700001701520700001701537700002601554700002601580700002301606700002701629700002301656700002101679700003101700700001801731700002101749700002001770700003001790700001501820700002001835700001901855700002501874700002301899700001901922700002101941700001701962700002201979700002102001700002602022700002302048700001102071700001702082700002202099700002102121700003102142700002402173700002202197700002202219700001802241700001702259700002502276700002202301700001902323700002702342700001602369700001802385700002202403700003202425700002302457700002302480700002002503700002202523700002002545700002702565700001802592700002102610700001902631700002302650700001502673700002202688700002302710700002202733700002402755700002002779700002102799700002302820700001802843700002702861856005402888 2019 eng d00aSoil nematode abundance and functional group composition at a global scale0 aSoil nematode abundance and functional group composition at a gl c08/20190 v5723 a

Soil organisms are a crucial part of the terrestrial biosphere. Despite their importance for ecosystem functioning, few quantitative, spatially explicit models of the active belowground community currently exist. In particular, nematodes are the most abundant animals on Earth, filling all trophic levels in the soil food web. Here we use 6,759 georeferenced samples to generate a mechanistic understanding of the patterns of the global abundance of nematodes in the soil and the composition of their functional groups. The resulting maps show that 4.4 ± 0.64 × 1020 nematodes (with a total biomass of approximately 0.3 gigatonnes) inhabit surface soils across the world, with higher abundances in sub-Arctic regions (38% of total) than in temperate (24%) or tropical (21%) regions. Regional variations in these global trends also provide insights into local patterns of soil fertility and functioning. These high-resolution models provide the first steps towards representing soil ecological processes in global biogeochemical models and will enable the prediction of elemental cycling under current and future climate scenarios.

1 avan den Hoogen, Johan1 aGeisen, Stefan1 aRouth, Devin1 aFerris, Howard1 aTraunspurger, Walter1 aWardle, D1 ade Goede, Ron, G. M.1 aAdams, Byron1 aAhmad, Wasim1 aAndriuzzi, Walter, S.1 aBardgett, Richard, D.1 aBonkowski, Michael1 aCampos-Herrera, Raquel1 aCares, Juvenil, E.1 aCaruso, Tancredi1 aCaixeta, Larissa, de Brito1 aChen, Xiaoyun1 aCosta, Sofia, R.1 aCreamer, Rachel1 aCastro, José, Mauro da C1 aDam, Marie1 aDjigal, Djibril1 aEscuer, Miguel1 aGriffiths, Bryan, S.1 aGutiérrez, Carmen1 aHohberg, Karin1 aKalinkina, Daria1 aKardol, Paul1 aKergunteuil, Alan1 aKorthals, Gerard1 aKrashevska, Valentyna1 aKudrin, Alexey, A.1 aLi, Qi1 aLiang, Wenju1 aMagilton, Matthew1 aMarais, Mariette1 aMartín, José, Antonio Ro1 aMatveeva, Elizaveta1 aMayad, El, Hassan1 aMulder, Christian1 aMullin, Peter1 aNeilson, Roy1 aNguyen, T., A. Duong1 aNielsen, Uffe, N.1 aOkada, Hiroaki1 aRius, Juan, Emilio Pal1 aPan, Kaiwen1 aPeneva, Vlada1 aPellissier, Loïc1 ada Silva, Julio, Carlos Per1 aPitteloud, Camille1 aPowers, Thomas, O.1 aPowers, Kirsten1 aQuist, Casper, W.1 aRasmann, Sergio1 aMoreno, Sara, Sánchez1 aScheu, Stefan1 aSetälä, Heikki1 aSushchuk, Anna1 aTiunov, Alexei, V.1 aTrap, Jean1 avan der Putten, W1 aVestergård, Mette1 aVillenave, Cecile1 aWaeyenberge, Lieven1 aWall, Diana, H.1 aWilschut, Rutger1 aWright, Daniel, G.1 aYang, Jiue-in1 aCrowther, Thomas, Ward uhttps://www.nature.com/articles/s41586-019-1418-602017nas a2200133 4500008004100000245011800041210006900159260005700228490000900285520149000294100002001784700002101804856005801825 2019 eng d00aSpatiotemporal impact of snow on underwater photosynthetically active radiation in Taylor Valley, East Antarctica0 aSpatiotemporal impact of snow on underwater photosynthetically a aBaton Rouge, LAbLouisiana State Universityc08/20190 vM.S.3 a

The role of snow on underwater photosynthetically active radiation (UW PAR) in the McMurdo Dry Valleys (MDVs) has been understudied due to lack of a detailed snowfall record. Research has shown that a relationship between snow cover and UW PAR exists, but the extent has never been evaluated in great detail. Although annual snowfall values in the MDVs are low (3 to 50 mm water equivalent annually), trends of increasing snowfall on the continent under future warming conditions could lead to an increased role for snow in regulating UW PAR (and associated primary productivity). Here, I discuss evidence from the snowfall record, surface PAR, and UW PAR, of the influence of snowfall on UW PAR in the major lakes of Taylor Valley. This study aims to quantify the spatiotemporal impact of lake ice snow packs on UW PAR in Taylor Valley from field surveys, long-term UW PAR, and meteorological data. Lake Fryxell has the strongest seasonality to precipitation, which decreases inland. On average, Lake Fryxell also has the most days with snow cover on the lake ice. Lake Hoare is experiencing an increase in Fall snow persistence since the 2007 snow year. Snow less than 0.5 mm snow water equivalent (SWE) can suppress UW PAR by 40%. The calendar day that snow falls often determines whether phototrophs will switch from photosynthesis to respiration, which suggests the importance to seasonality in determining the impact of snow on photoautotrophs and lake-wide carbon budget.

1 aMyers, Madeline1 aDoran, Peter, T. uhttps://digitalcommons.lsu.edu/gradschool_theses/496502534nas a2200229 4500008004100000245010900041210006900150260001200219300001600231490000800247520179600255100002102051700001702072700002402089700001502113700002302128700001902151700002402170700001902194700001702213856007402230 2018 eng d00aAeolian material transport and its role in landscape connectivity in the McMurdo Dry Valleys, Antarctica0 aAeolian material transport and its role in landscape connectivit c12/2018 a3323 - 33370 v1233 a

Arid regions, particularly polar and alpine desert environments, have diminished landscape connectivity compared to temperate regions due to limited and/or seasonal hydrological processes. For these environments, aeolian processes play a particularly important role in landscape evolution and biotic community vitality through nutrient and solute additions. The McMurdo Dry Valleys (MDV) are the largest ice-free area in Antarctica and are potentially a major source of aeolian material for the continent. From this region, samples were collected at five heights (~5, 10, 20, 50, and 100 cm) above the surface seasonally for 2013 through 2015 from Alatna Valley, Victoria Valley, Miers Valley, and Taylor Valley (Taylor Glacier, East Lake Bonney, F6 (Lake Fryxell), and Explorer’s Cove). Despite significant geological separation and varying glacial histories, low-elevation and coastal sites had similar major ion chemistries, as did high-elevation and inland locations. This locational clustering of compositions was also evident in scanning electron microscopy images and principal component analyses, particularly for samples collected at ~100 cm above the surface. Compared to published soil literature, aeolian material in Taylor Valley demonstrates a primarily down-valley transport of material toward the coast. Soluble N:P ratios in the aeolian material reflect relative nutrient enrichments seen in MDV soils and lakes, where younger, coastal soils are relatively N depleted, while older, up-valley soils are relatively P depleted. The aeolian transport of materials, including water-soluble nutrients, is an important vector of connectivity within the MDV and provides a mechanism to help “homogenize” the geochemistry of both soil and aquatic ecosystems.

1 aDiaz, Melisa, A.1 aAdams, Byron1 aWelch, Kathleen, A.1 aWelch, Sue1 aOpiyo, Stephen, O.1 aKhan, Alia, L.1 aMcKnight, Diane, M.1 aCary, Craig, S1 aLyons, Berry uhttps://agupubs.onlinelibrary.wiley.com/doi/full/10.1029/2017JF00458902006nas a2200133 4500008004100000245016300041210006900204260003900273490000900312520143800321100001501759700002901774856006901803 2018 eng d00aAntarctic Chlamydomonas strains C. sp. UWO241 and ICE-MDV exhibit differential restructuring of the photosynthetic apparatus in response to iron0 aAntarctic iChlamydomonasi strains iCi sp UWO241 and ICEMDV exhib aOxford, OHbMiami Universityc20180 vM.S.3 a

As an integral cofactor for many redox-associated processes, iron (Fe) homeostasis is crucial in order to produce sufficient energy for the organism. Fe limitation, or excess, can cause major alterations in the function and structure of the photosynthetic apparatus. Photosynthetic psychrophiles grown under permanent low temperatures exhibit novel adaptations in their photosynthetic apparatus to deal with this permanent stress. The ice-covered lakes of the McMurdo Dry Valleys harbor many species of cold-adapted algae, including Chlamydomonas sp. UWO241 (UWO241). As a consequence of adaptation to multiple permanent extreme conditions, UWO241 exhibits a remodeled photosynthetic apparatus for maintaining redox poise. One unusual characteristic of UWO241 is the absence of a PSI-associated 77K fluorescence emission under a wide range of growth conditions. This phenotype resembles Fe deficiency in other model organisms such as C. reinhardtii. We hypothesized that adaptation to permanent iron deficiency in its native environment may contribute to this unusual phenotype. We compared the effect of Fe availability on the physiology and photobiology of UWO241 with the model C. reinhardtii as well as a second psychrophilic alga, Chlamydomonas sp. ICE-MDV (ICE). The impacts of a restructured photosynthetic apparatus on the unique Fe-associated phenotype in UWO241 will be discussed.

1 aCook, Greg1 aMorgan-Kiss, Rachael, M. uhttp://rave.ohiolink.edu/etdc/view?acc_num=miami152545562177883602748nas a2200241 4500008004100000245014000041210006900181260004600250300000800296490001000304520196700314653001502281653002102296653002602317653001502343653001902358653001202377653001402389653000902403100001902412700002402431856005102455 2018 eng d00aBiogeochemistry, contaminant transport, and atmospheric exchange in glacial cryoconite meltwater of the McMurdo Dry Valleys, Antarctica0 aBiogeochemistry contaminant transport and atmospheric exchange i aBoulder, CObUniversity of Coloradoc2018 a2450 vPh.D.3 a

Polar regions serve as a global sink for many forms of semi-volatile pollution emitted from low- or midlatitudes of the populated world. This study examined the longrange atmospheric transport, fate, and phase partitioning of semi-volatile organic contaminants from air masses into meltwater and aeolian sediment on six glaciers in the McMurdo Dry Valleys of Antarctica. A novel low-cost, field-portable instrument was developed for the in-situ solid-phase extraction of trace contaminants in extreme environmental conditions without access to electricity or traditional laboratory facilities. Beyond polar research, this equipment is applicable for rapid field extraction and stabilization of samples assessing air and water quality after natural disasters. This is the first published study to identify the presence of anthropogenic perfluorinated compounds in the Transantarctic Mountain region and indicates a longer range of poleward contaminant transport than prior estimates in the Southern Hemisphere. Additional research examined the biochemistry and climatic variability of open and sealed cryoconite holes on glacial surfaces throughout the initial melt, equilibrium, and refreezing periods in 2013–2015. High solute concentrations relative to glacial ice indicate that the pools can remain isolated from hydrologic connectivity for more than a decade. Microbial carbon cycling in pools enclosed by ice led to atmospheric disequilibrium and extreme pH. Analysis of unique air, liquid, and ice stratification in cryoconite holes revealed vertical patterns representing a highly accurate, multi-year record of past weather conditions sensitive enough to identify individual dates. This research identifies fluctuations in atmospheric contaminant transport, specific timeframes for deposition events, and may be used in back-trajectory models to help identify the source and variability of semi-volatile emissions in the Southern hemisphere.

10aAntarctica10aapplied sciences10acontaminant transport10acryoconite10aearth sciences10aglacier10ahydrology10amelt1 aMass, Alex, Q.1 aMcKnight, Diane, M. uhttps://search.proquest.com/docview/204831467810050nas a2202965 4500008004100000245007300041210006900114260001200183300001200195490000700207520154900214653001701763653001101780653001201791653002101803653001301824653001301837100002001850700002201870700001601892700002201908700002301930700001701953700002801970700002001998700002502018700001802043700002402061700001602085700002202101700002002123700002702143700002002170700002202190700002302212700003102235700002002266700002302286700002702309700002202336700002702358700002702385700001602412700001902428700002302447700002102470700002202491700001802513700002302531700002802554700002502582700002302607700002002630700002902650700002502679700002002704700002602724700002602750700001702776700002402793700002502817700002302842700002202865700001902887700002002906700002102926700002202947700002102969700001802990700001703008700002203025700001703047700002503064700001903089700002603108700002203134700001903156700002103175700001903196700002703215700003003242700002403272700002403296700001703320700002303337700003203360700002103392700002403413700002203437700002903459700001403488700003103502700002303533700001903556700002303575700002603598700001803624700002103642700001603663700001803679700002403697700002103721700002003742700001903762700001703781700001903798700002103817700002003838700002603858700002003884700002003904700002003924700002503944700002003969700002703989700002404016700003304040700001804073700001504091700002304106700002204129700002004151700001704171700002404188700001804212700002404230700002104254700002204275700002104297700002004318700002004338700002804358700002604386700001704412700002104429700001904450700002304469700003104492700002604523700002004549700002704569700002204596700002104618700002204639700002004661700002804681700001804709700002204727700002604749700002004775700001904795700002404814700001804838700002404856700002604880700001804906700002304924700002504947700002804972700002205000700002505022700001505047700001705062700001505079700003105094700001905125700001805144700002605162700002405188700001905212700001805231700002205249700002605271700002405297700002005321700002405341700002105365700002905386700002105415700002205436700002005458700001905478700002405497700002505521700002105546700002805567700001905595700002205614700001505636700002605651700002105677700002105698700002505719700002205744700001905766700002805785700002305813700002605836700002105862700002105883700002705904700002805931700002105959700001905980700001905999700003006018700002106048700002406069700003206093700003606125700001806161700001606179700002206195700002306217700001906240700001806259700001506277700002106292700002606313700003306339700001706372700002006389700002106409700002406430700002106454700002206475700001906497700002306516700002106539700001806560700002806578700003406606700002406640700002106664700001806685700002006703700003206723700002606755700002706781700002906808700003106837700002106868700002306889700002506912700002106937700002306958700002506981700001607006856006207022 2018 eng d00aBioTIME: A database of biodiversity time series for the Anthropocene0 aBioTIME A database of biodiversity time series for the Anthropoc c07/2018 a760-7860 v273 a

Motivation: The BioTIME database contains raw data on species identities and abundances in ecological assemblages through time. These data enable users to calculate temporal trends in biodiversity within and amongst assemblages using a broad range of metrics. BioTIME is being developed as a community-led open-source database of biodiversity time series. Our goal is to accelerate and facilitate quantitative analysis of temporal patterns of biodiversity in the Anthropocene.

Main types of variables included: The database contains 8,777,413 species abundance records, from assemblages consistently sampled for a minimum of 2 years, which need not necessarily be consecutive. In addition, the database contains metadata relating to sampling methodology and contextual information about each record.

Spatial location and grain: BioTIME is a global database of 547,161 unique sampling locations spanning the marine, freshwater and terrestrial realms. Grain size varies across datasets from 0.0000000158 km2 (158 cm2) to 100 km2 (1,000,000,000,000 cm2). Time period and grain BioTIME records span from 1874 to 2016. The minimal temporal grain across all datasets in BioTIME is a year.

Major taxa and level of measurement: BioTIME includes data from 44,440 species across the plant and animal kingdoms, ranging from plants, plankton and terrestrial invertebrates to small and large vertebrates.

Software format: .csv and .SQL.

10abiodiversity10aglobal10aspatial10aspecies richness10atemporal10aturnover1 aDornelas, Maria1 aAntão, Laura, H.1 aMoyes, Faye1 aBates, Amanda, E.1 aMagurran, Anne, E.1 aAdam, Dušan1 aAkhmetzhanova, Asem, A.1 aAppeltans, Ward1 aArcos, José, Manuel1 aArnold, Haley1 aAyyappan, Narayanan1 aBadihi, Gal1 aBaird, Andrew, H.1 aBarbosa, Miguel1 aBarreto, Tiago, Egydio1 aBässler, Claus1 aBellgrove, Alecia1 aBelmaker, Jonathan1 aBenedetti-Cecchi, Lisandro1 aBett, Brian, J.1 aBjorkman, Anne, D.1 aBłażewicz, Magdalena1 aBlowes, Shane, A.1 aBloch, Christopher, P.1 aBonebrake, Timothy, C.1 aBoyd, Susan1 aBradford, Matt1 aBrooks, Andrew, J.1 aBrown, James, H.1 aBruelheide, Helge1 aBudy, Phaedra1 aCarvalho, Fernando1 aCastañeda-Moya, Edward1 aChen, Chaolun, Allen1 aChamblee, John, F.1 aChase, Tory, J.1 aCollier, Laura, Siegwart1 aCollinge, Sharon, K.1 aCondit, Richard1 aCooper, Elisabeth, J.1 aCornelissen, Hans, C.1 aCotano, Unai1 aCrow, Shannan, Kyle1 aDamasceno, Gabriella1 aDavies, Claire, H.1 aDavis, Robert, A.1 aDay, Frank, P.1 aDegraer, Steven1 aDoherty, Tim, S.1 aDunn, Timothy, E.1 aDurigan, Giselda1 aDuffy, Emmett1 aEdelist, Dor1 aEdgar, Graham, J.1 aElahi, Robin1 aElmendorf, Sarah, C.1 aEnemar, Anders1 aErnest, S., K. Morgan1 aEscribano, Rubén1 aEstiarte, Marc1 aEvans, Brian, S.1 aFan, Tung-Yung1 aFarah, Fabiano, Turini1 aFernandes, Luiz, Loureiro1 aFarneda, Fábio, Z.1 aFidelis, Alessandra1 aFitt, Robert1 aFosaa, Anna, Maria1 aFranco, Geraldo, Antonio Da1 aFrank, Grace, E.1 aFraser, William, R.1 aGarcía, Hernando1 aGatti, Roberto, Cazzolla1 aGivan, Or1 aGorgone-Barbosa, Elizabeth1 aGould, William, A.1 aGries, Corinna1 aGrossman, Gary, D.1 aGutierréz, Julio, R.1 aHale, Stephen1 aHarmon, Mark, E.1 aHarte, John1 aHaskins, Gary1 aHenshaw, Donald, L.1 aHermanutz, Luise1 aHidalgo, Pamela1 aHiguchi, Pedro1 aHoey, Andrew1 aVan Hoey, Gert1 aHofgaard, Annika1 aHoleck, Kristen1 aHollister, Robert, D.1 aHolmes, Richard1 aHoogenboom, Mia1 aHsieh, Chih-hao1 aHubbell, Stephen, P.1 aHuettmann, Falk1 aHuffard, Christine, L.1 aHurlbert, Allen, H.1 aIvanauskas, Natália, Macedo1 aJaník, David1 aJandt, Ute1 aJażdżewska, Anna1 aJohannessen, Tore1 aJohnstone, Jill1 aJones, Julia1 aJones, Faith, A. M.1 aKang, Jungwon1 aKartawijaya, Tasrif1 aKeeley, Erin, C.1 aKelt, Douglas, A.1 aKinnear, Rebecca1 aKlanderud, Kari1 aKnutsen, Halvor1 aKoenig, Christopher, C.1 aKortz, Alessandra, R.1 aKrál, Kamil1 aKuhnz, Linda, A.1 aKuo, Chao-Yang1 aKushner, David, J.1 aLaguionie-Marchais, Claire1 aLancaster, Lesley, T.1 aLee, Cheol, Min1 aLefcheck, Jonathan, S.1 aLévesque, Esther1 aLightfoot, David1 aLloret, Francisco1 aLloyd, John, D.1 aLópez-Baucells, Adrià1 aLouzao, Maite1 aMadin, Joshua, S.1 aMagnússon, Borgþór1 aMalamud, Shahar1 aMatthews, Iain1 aMcFarland, Kent, P.1 aMcGill, Brian1 aMcKnight, Diane, M.1 aMcLarney, William, O.1 aMeador, Jason1 aMeserve, Peter, L.1 aMetcalfe, Daniel, J.1 aMeyer, Christoph, F. J.1 aMichelsen, Anders1 aMilchakova, Nataliya1 aMoens, Tom1 aMoland, Even1 aMoore, Jon1 aMoreira, Carolina, Mathias1 aMüller, Jörg1 aMurphy, Grace1 aMyers-Smith, Isla, H.1 aMyster, Randall, W.1 aNaumov, Andrew1 aNeat, Francis1 aNelson, James, A.1 aNelson, Michael, Paul1 aNewton, Stephen, F.1 aNorden, Natalia1 aOliver, Jeffrey, C.1 aOlsen, Esben, M.1 aOnipchenko, Vladimir, G.1 aPabis, Krzysztof1 aPabst, Robert, J.1 aPaquette, Alain1 aPardede, Sinta1 aPaterson, David, M.1 aPélissier, Raphaël1 aPeñuelas, Josep1 aPérez-Matus, Alejandro1 aPizarro, Oscar1 aPomati, Francesco1 aPost, Eric1 aPrins, Herbert, H. T.1 aPriscu, John, C.1 aProvoost, Pieter1 aPrudic, Kathleen, L.1 aPulliainen, Erkki1 aRamesh, B., R.1 aRamos, Olivia, Mendivil1 aRassweiler, Andrew1 aRebelo, Jose, Eduardo1 aReed, Daniel, C.1 aReich, Peter, B.1 aRemillard, Suzanne, M.1 aRichardson, Anthony, J.1 aRichardson, Paul1 avan Rijn, Itai1 aRocha, Ricardo1 aRivera-Monroy, Victor, H.1 aRixen, Christian1 aRobinson, Kevin, P.1 aRodrigues, Ricardo, Ribeiro1 aRossa-Feres, Denise, de Cerquei1 aRudstam, Lars1 aRuhl, Henry1 aRuz, Catalina, S.1 aSampaio, Erica, M.1 aRybicki, Nancy1 aRypel, Andrew1 aSal, Sofia1 aSalgado, Beatriz1 aSantos, Flavio, A. M.1 aSavassi-Coutinho, Ana, Paula1 aScanga, Sara1 aSchmidt, Jochen1 aSchooley, Robert1 aSetiawan, Fakhrizal1 aShao, Kwang-Tsao1 aShaver, Gaius, R.1 aSherman, Sally1 aSherry, Thomas, W.1 aSiciński, Jacek1 aSievers, Caya1 ada Silva, Ana, Carolina1 ada Silva, Fernando, Rodrigues1 aSilveira, Fabio, L.1 aSlingsby, Jasper1 aSmart, Tracey1 aSnell, Sara, J.1 aSoudzilovskaia, Nadejda, A.1 aSouza, Gabriel, B. G.1 aSouza, Flaviana, Maluf1 aSouza, Vinícius, Castro1 aStallings, Christopher, D.1 aStanforth, Rowan1 aStanley, Emily, H.1 aSterza, José, Mauro1 aStevens, Maarten1 aStuart-Smith, Rick1 aSuarez, Yzel, Rondon1 aSupp, Sarah uhttps://onlinelibrary.wiley.com/doi/abs/10.1111/geb.1272901794nas a2200181 4500008004100000245015800041210006900199260001200268300001400280490000600294520113500300100002201435700002101457700001601478700002201494700002401516856007201540 2018 eng d00aCatch and release: Hyporheic retention and mineralization of N-fixing Nostoc sustains downstream microbial mat biomass in two polar desert streams0 aCatch and release Hyporheic retention and mineralization of Nfix c07/2018 a357 - 3640 v33 a

 

Much work has been performed to investigate controls on nitrogen (N) uptake in streams, yet the fate of assimilated N is comparatively poorly resolved. Here, we use in-stream fixed N as an isotopic tracer to study the fate of assimilated N in glacial meltwater streams. We characterized d15N signatures of Oscillatorean, Chlorophyte, and N-fixing Nostoc mats over the lengths of two streams, and transported particulate organic matter (POM) in one. POM was isotopically most similar to Nostoc, which always had values near the atmospheric standard, suggesting N-fixation. Other mat types were depleted upstream, and became progressively enriched downstream, indicating a shift in N source. These results collectively show that Nostoc-derived N is mobilized, mineralized, and increasingly assimilated downstream as more depleted glacier-derived N is exhausted, demonstrating the importance of organic matter processing to balancing elemental budgets, and improving our understanding of nutrient cycling in lotic environments.

 

1 aKohler, Tyler, J.1 aStanish, Lee, F.1 aLiptzin, D.1 aBarrett, John, E.1 aMcKnight, Diane, M. uhttps://aslopubs.onlinelibrary.wiley.com/doi/abs/10.1002/lol2.1008702633nas a2200133 4500008004100000245016300041210006900204260005700273490000900330520205900339100002202398700002102420856005802441 2018 eng d00aGroundwater and thermal legacy of a large paleolake in Taylor Valley, East Antarctica as evidenced by airborne electromagnetic and sedimentological techniques0 aGroundwater and thermal legacy of a large paleolake in Taylor Va aBaton Rouge, LAbLouisiana State Universityc07/20180 vM.S.3 a

During the Last Glacial Maximum, grounded ice in the Ross Sea extended into the otherwise ice-free McMurdo Dry Valleys, creating a series of large ice dammed paleolakes. Grounded ice within the mouth of Taylor Valley allowed for lake levels to reach elevations not possible at modern day and formed what is known as Glacial Lake Washburn (GLW). GLW extended from the eastern portion of Taylor Valley roughly 20 km west to a level ~300 m higher than modern day Lake Fryxell. The formation and existence of GLW has been debated, though previous studies correlate the timing of GLW with early Holocene grounded ice. Evidence of GLW has largely been constrained to the interpretation of glacial deposits and fluvial features such as lacustrine deposits, strandlines, and preserved paleodeltas. GIS and remote sensing techniques paired with regional resistivity data provide new insight into the paleohydrology of the region.

To quantify the extent of GLW, paleodelta locations were mapped using high resolution LiDAR digital elevation models and satellite imagery. Delta topset elevations were correlated between three streams in Fryxell basin to determine paleolake levels. Additionally, mean resistivity maps generated from airborne electromagnetic survey data (SkyTEM) reveal an extensive groundwater system within Fryxell basin which is interpreted as a legacy groundwater signal from GLW. Resistivity data suggests that active permafrost formation has been ongoing since onset of lake drainage, and that lake levels were over 60 m higher than modern only 1,000 – 2,000 yr BP. This coincides with a warmer than modern paleoclimate inferred by ice core records, indicating a dynamic hydrological system that is highly sensitive to small changes in climate. As global temperatures increase, Lake Fryxell will continue to experience highly variable lake levels. Lakes and groundwater within the McMurdo Dry Valleys are critical to understanding impacts on the broader ecosystem which is largely driven by the availability of liquid water.

1 aMyers, Krista, F.1 aDoran, Peter, T. uhttps://digitalcommons.lsu.edu/gradschool_theses/477602931nas a2200205 4500008004100000245011300041210006900154260001200223490000600235520222200241100002202463700002402485700002402509700002302533700002402556700002102580700002102601700003302622856007002655 2018 eng d00aLocal and Regional Scale Heterogeneity Drive Bacterial Community Diversity and Composition in a Polar Desert0 aLocal and Regional Scale Heterogeneity Drive Bacterial Community c08/20180 v93 a

The distribution of organisms in an environment is neither uniform nor random but is instead spatially patterned. The factors that control this patterning are complex and the underlying mechanisms are poorly understood. Soil microbes are critical to ecosystem function but exhibit highly complex distributions and community dynamics due in large part to the scale-dependent effects of environmental heterogeneity. To better understand the impact of environmental heterogeneity on the distribution of soil microbes, we sequenced the 16S rRNA gene from bacterial communities in the microbe-dominated polar desert ecosystem of the McMurdo Dry Valleys (MDV), Antarctica. Significant differences in key edaphic variables and alpha diversity were observed among the three lake basins of the Taylor Valley (Kruskal–Wallis; pH: χ2 = 68.89, P < 0.001, conductivity: χ2 = 35.03, P < 0.001, observed species: χ2 = 7.98, P = 0.019 and inverse Simpson: χ2 = 18.52, P < 0.001) and each basin supported distinctive microbial communities (ANOSIM R = 0.466, P = 0.001, random forest ratio of 14.1). However, relationships between community structure and edaphic characteristics were highly variable and contextual, ranging in magnitude and direction across regional, basin, and local scales. Correlations among edaphic factors (pH and soil conductivity) and the relative abundance of specific phyla were most pronounced along local environmental gradients in the Lake Fryxell basin where Acidobacteria, Bacteroidetes, and Proteobacteria declined while Deinococcus–Thermus and Gemmatimonadetes increased with soil conductivity (all P < 0.1). Species richness was most strongly related to the soil conductivity gradient present within this study system. We suggest that the relative importance of pH versus soil conductivity in structuring microbial communities is related to the length of edaphic gradients and the spatial scale of sampling. These results highlight the importance of conducting studies over large ranges of key environmental gradients and across multiple spatial scales to assess the influence of environmental heterogeneity on the composition and diversity of microbial communities.

1 aFeeser, Kelli, L.1 aVan Horn, David, J.1 aBuelow, Heather, N.1 aColman, Daniel, R.1 aMcHugh, Theresa, A.1 aOkie, Jordan, G.1 aSchwartz, Egbert1 aTakacs-Vesbach, Cristina, D. uhttps://www.frontiersin.org/article/10.3389/fmicb.2018.01928/full04038nas a2200205 4500008004100000245015700041210006900198260001200267300001600279490000800295520333900303100001903642700002103661700002403682700001503706700002403721700001703745700002403762856004603786 2018 eng d00aNear-surface refractory black carbon observations in the atmosphere and snow in the McMurdo Dry Valleys, Antarctica and potential impacts of foehn winds0 aNearsurface refractory black carbon observations in the atmosphe c01/2018 a2877 - 28870 v1233 a

Measurements of light absorbing particles in the boundary layer of the high southern latitudes are scarce, particularly in the McMurdo Dry Valleys (MDV), Antarctica. During the 2013 - 2014 austral summer near-surface boundary layer refractory black carbon (rBC) aerosols were measured in air by a single particle soot photometer (SP2) at multiple locations in the MDV. Near-continuous rBC atmospheric measurements were collected at Lake Hoare Camp (LH) over two months and for several hours at more remote locations away from established field camps. We investigated periods dominated by both up and down-valley winds to explore the causes of differences in rBC concentrations and size distributions. Snow samples were also collected in a 1m pit on a glacier near the camp. The range of concentrations rBC in snow were 0.3 1.2 ± 0.3 μg-rBC/L-H2O, and total organic carbon were 0.3 1.4 ± 0.3 mg/L. The rBC concentrations measured in this snow pit are not sufficient to reduce surface albedo, however, there is potential for accumulation of rBC on snow and ice surfaces at low elevation throughout the MDV which were not measured as part of this study. At LH, the average background rBC mass aerosol concentrations was 1.3 ng/m3. rBC aerosol mass concentrations were slightly lower, 0.09 1.3 ng/m3, at the most remote sites in the MDV. Concentration spikes as high as 200 ng/m3 were observed at LH, associated with local activities. During a foehn wind event, the average rBC mass concentration increased to 30-50 ng m-3. Here we show the rBC increase could be due to resuspension of locally produced BC from generators, rocket toilets, and helicopters, which may remain on the soil surface until redistributed during high wind events. Quantification of local production and long-range atmospheric transport of rBC to the MDV is necessary for understanding the impacts of this species on regional climate. 

 

1 aKhan, Alia, L.1 aMcMeeking, Gavin1 aSchwarz, Joshua, P.1 aXian, Peng1 aWelch, Kathleen, A.1 aLyons, Berry1 aMcKnight, Diane, M. uhttp://doi.wiley.com/10.1002/2017JD02769602179nas a2200121 4500008004100000245006300041210006300104260002600167520172200193100002201915700002901937856009101966 2018 eng d00aPhysiological and Biochemical Adaptations of Psychrophiles0 aPhysiological and Biochemical Adaptations of Psychrophiles aBoca RatonbCRC Press3 a

The cold biosphere encompasses many microorganism-dominated habitats that rely on light-dependent primary production. Within these environments, there are numerous physical and chemical factors limiting metabolism and growth that the microorganisms must overcome. The psychrophilic microorganisms discussed herein integrate a complex spectrum of adaptive strategies to survive these physiological challenges, including genome evolution, enzyme structure and catalysis rate changes, cryoprotectant formation, and a multitude of photosynthetic adaptations. Psychrophilic organisms also hold the key to biotechnical advances and the future, such that psychrophilic enzymes are used for everything from laboratory reagents and industrial work to medical research and environmental sustainability. Researchers have learned to exploit psychrophiles’ efficiency at low temperatures (i.e., cooler washing machines and energy-saving, cost-effective enzyme production), their higher energy activity (thus allowing lower concentrations of needed catalysts, reducing costs and procedure time), and their ability to contribute to hydrocarbon bioremediation. Although psychrophilic microbes exist in numerous habitats and undergo various adaptive strategies, an understanding of what makes an organism psychrophilic is still unknown in a large majority of cold-adapted organisms, and thus future investigations are needed regarding cold adaptation and their biotechnological potential. Even as research has increased over the last decade, new technological advances and high-throughput DNA sequencing will continue to provide information about cold adaptation or the mechanisms needed for survival in a changing world.

1 aTeufel, Amber, G.1 aMorgan-Kiss, Rachael, M. uhttps://www.taylorfrancis.com/books/e/9781498774932/chapters/10.1201%2F9781315154695-902513nas a2200277 4500008004100000245010700041210006900148260001200217300001600229490000700245520169000252653001201942653001601954653001601970653001401986653001502000653001602015100002602031700002102057700002402078700001702102700001702119700002002136700002402156856005502180 2018 eng d00aSpatial and temporal patterns of microbial mats and associated invertebrates along an Antarctic stream0 aSpatial and temporal patterns of microbial mats and associated i c10/2018 a1911–19210 v413 a

 

Microbial biofilms are biological hotspots in many alpine and polar ecosystems, but the controls on and functional significance of their fauna are little known. We studied cyanobacterial mats and the underlying sediment in a glacial meltwater stream in the McMurdo Dry Valleys, Antarctica. We investigated mat biomass (total and phototrophic), diatoms, and micro-meiofauna (nematodes, rotifers, and tardigrades) at nine sites along a 1670 m stream reach in a cold, low-flow growing season, and in a warmer growing season in which peak flows (above 100 L s−1) scoured the mats. Diatom and invertebrate communities were not related, but mat biomass in the low-flow year was negatively related to nematode abundance, including that of the omnivore Eudorylaimus. In the high-flow year that followed, invertebrate abundance was reduced in the mats, diatom community structure was altered, and mat biomass was higher. The difference in invertebrate abundance between years was greater in mats in upstream reaches, where the greatest increases in flow velocity may have occurred, and was negligible in mats in downstream reaches as well as in the sediment beneath the mats. Integrating our results with previous findings, we generate two predictive hypotheses to be tested in glacial meltwater streams: (1) under peak flows invertebrates decline in the microbial mats, while (2) the sediment beneath the mats is a refuge from the flow disturbance. Our results also suggest that, under stable flow conditions, microinvertebrate grazers could exert top-down control on microbial mat biomass.

 

10adiatoms10aDisturbance10aDry valleys10aEpilithon10aMicrofauna10aStream flow1 aAndriuzzi, Walter, S.1 aStanish, Lee, F.1 aSimmons, Breana, L.1 aJaros, Chris1 aAdams, Byron1 aWall, Diana, H.1 aMcKnight, Diane, M. uhttp://link.springer.com/10.1007/s00300-018-2331-402975nas a2200289 4500008004100000245013500041210006900176260001200245490000600257520199400263653002902257653002302286653002402309653003102333653002602364653002602390100002602416700002402442700001602466700002002482700002202502700002602524700002502550700002302575700001702598856007002615 2018 eng d00aStoichiometric Shifts in Soil C:N:P Promote Bacterial Taxa Dominance, Maintain Biodiversity, and Deconstruct Community Assemblages0 aStoichiometric Shifts in Soil CNP Promote Bacterial Taxa Dominan c07/20180 v93 a

Imbalances in C:N:P supply ratios may cause bacterial resource limitations and constrain biogeochemical processes, but the importance of shifts in soil stoichiometry are complicated by the nearly limitless interactions between an immensely rich species pool and a multiple chemical resource forms. To more clearly identify the impact of soil C:N:P on bacteria, we evaluated the cumulative effects of single and coupled long-term nutrient additions (i.e., C as mannitol, N as equal concentrations NH4 + and NO3 − , and P as Na3PO4) and water on communities in an Antarctic polar desert, Taylor Valley. Untreated soils possessed relatively low bacterial diversity, simplified organic C sources due to the absence of plants, limited inorganic N, and excess soil P potentially attenuating links between C:N:P. After 6 years of adding resources, an alleviation of C and N colimitation allowed one rare Micrococcaceae, an Arthrobacter species, to dominate, comprising 47% of the total community abundance and elevating soil respiration by 136% relative to untreated soils. The addition of N alone reduced C:N ratios, elevated bacterial richness and diversity, and allowed rare taxa relying on ammonium and nitrite for metabolism to become more abundant [e.g., nitrite oxidizing Nitrospira species (Nitrosomonadaceae), denitrifiers utilizing nitrite (Gemmatimonadaceae) and members of Rhodobacteraceae with a high affinity for ammonium]. Based on community co-occurrence networks, lower C:P ratios in soils following P and CP additions created more diffuse and less connected communities by disrupting 73% of species interactions and selecting for taxa potentially exploiting abundant P. Unlike amended nutrients, water additions alone elicited no lasting impact on communities. Our results suggest that as soils become nutrient rich a wide array of outcomes are possible from species dominance and the deconstruction of species interconnectedness to the maintenance of biodiversity.

10aecological stoichiometry10aLake Fryxell Basin10aMcMurdo Dry Valleys10anetwork community modeling10anutrient colimitation10aSolirubrobacteriaceae1 aAanderud, Zachary, T.1 aSaurey, Sabrina, D.1 aBall, Becky1 aWall, Diana, H.1 aBarrett, John, E.1 aMuscarella, Mario, E.1 aGriffin, Natasha, A.1 aVirginia, Ross, A.1 aAdams, Byron uhttps://www.frontiersin.org/article/10.3389/fmicb.2018.01401/full01835nas a2200169 4500008004100000245013700041210006900178260001200247300001800259490000700277520122000284100002101504700002501525700002401550700001701574856007401591 2018 eng d00aTransit times and rapid chemical equilibrium explain chemostasis in glacial meltwater streams in the McMurdo Dry Valleys, Antarctica0 aTransit times and rapid chemical equilibrium explain chemostasis c12/2018 a13322 - 133310 v453 a

Fluid  transit  time  is  understood  to  be  an  important  control  on  the  shape  of concentration-discharge (C-q) relationships, yet empirical evidence supporting this linkage is limited. We investigated C-q relationships for weathering-derived solutes across seven Antarctic glacial meltwate streams. We hypothesized that (H1) solute fluxes in McMurdo Dry Valley streams are reaction limited so that C-q relationships are characterized by dilution and that (H2) transit time explains between-stream variability in the degree of C-q dilution. Results show that C-q relationships are chemostatic because solute equilibrium times are shorter than stream corridor fluid transit times. Between-stream variability in the efficiency of solute production is positively correlated with transit time, suggesting that transit time is an important control on the solute export regime. These results provide empirical evidence for the controls on weathering-derived C-q relationships and have important implications for Antarctic ecosystems and solute export regimes of watersheds worldwide.

1 aWlostowski, Adam1 aGooseff, Michael, N.1 aMcKnight, Diane, M.1 aLyons, Berry uhttps://agupubs.onlinelibrary.wiley.com/doi/full/10.1029/2018GL08036901371nas a2200157 4500008004100000020002200041245012600063210006900189250000600258260001300264520081000277100001701087700001801104700002401122856006701146 2017 eng d a978-3-319-57056-300aThe Climate of Snow and Ice as Boundary Condition for Microbial Life in Psychrophiles: From Biodiversity to Biotechnology0 aClimate of Snow and Ice as Boundary Condition for Microbial Life a2 bSpringer3 a

The microclimate and structure of snow and ice are a boundary condition as well as a matrix for a large spectrum of microbial life under alpine and polar conditions. Biological activity critically depends on the supply of energy, water and nutrients, with solar radiation as the prime source of energy, varying with latitude and altitude. The energy balance at the snow or ice surface provides the boundary condition for the fluxes of energy and water to the snow and ice, with important latitudinal differences from the temperate to the polar regions. The extreme situations of sunlit rocks surrounded by snow and the environment of Antarctic cryoconite holes, where ice, water, solar radiation and nutrients interact in particular ways, closes this review on ice and its effect on microbial life.

1 aMargesin, R.1 aKuhn, Michael1 aFountain, Andrew, G uhttp://link.springer.com/content/pdf/10.1007/978-3-319-57057-002880nas a2200625 4500008004100000245007400041210006900115260001200184490000800196520106500204100002301269700002101292700002101313700001601334700001801350700002301368700002201391700002301413700002201436700002001458700002701478700002001505700002301525700002801548700002301576700002201599700002101621700002401642700002001666700002401686700001601710700001501726700001801741700002201759700002701781700001901808700002401827700002201851700002101873700002801894700002201922700002201944700001701966700002101983700001902004700002202023700001802045700002702063700002302090700002302113700002202136700001602158700003202174856004802206 2017 eng d00aA communal catalogue reveals Earth’s multiscale microbial diversity0 acommunal catalogue reveals Earth s multiscale microbial diversit c11/20170 v5513 a

Our growing awareness of the microbial world’s importance and diversity contrasts starkly with our limited understanding of its fundamental structure. Despite recent advances in DNA sequencing, a lack of standardized protocols and common analytical frameworks impedes comparisons among studies, hindering the development of global inferences about microbial life on Earth. Here we present a meta-analysis of microbial community samples collected by hundreds of researchers for the Earth Microbiome Project. Coordinated protocols and new analytical methods, particularly the use of exact sequences instead of clustered operational taxonomic units, enable bacterial and archaeal ribosomal RNA gene sequences to be followed across multiple studies and allow us to explore patterns of diversity at an unprecedented scale. The result is both a reference database giving global context to DNA sequence data and a framework for incorporating data from future studies, fostering increasingly complete characterization of Earth’s microbial diversity.

1 aThompson, Luke, R.1 aSanders, Jon, G.1 aMcDonald, Daniel1 aAmir, Amnon1 aLadau, Joshua1 aLocey, Kenneth, J.1 aPrill, Robert, J.1 aTripathi, Anupriya1 aGibbons, Sean, M.1 aAckermann, Gail1 aNavas-Molina, Jose, A.1 aJanssen, Stefan1 aKopylova, Evguenia1 aVázquez-Baeza, Yoshiki1 aGonzález, Antonio1 aMorton, James, T.1 aMirarab, Siavash1 aXu, Zhenjiang, Zech1 aJiang, Lingjing1 aHaroon, Mohamed, F.1 aKanbar, Jad1 aZhu, Qiyun1 aSong, Se, Jin1 aKosciolek, Tomasz1 aBokulich, Nicholas, A.1 aLefler, Joshua1 aBrislawn, Colin, J.1 aHumphrey, Gregory1 aOwens, Sarah, M.1 aHampton-Marcell, Jarrad1 aBerg-Lyons, Donna1 aMcKenzie, Valerie1 aFierer, Noah1 aFuhrman, Jed, A.1 aClauset, Aaron1 aStevens, Rick, L.1 aShade, Ashley1 aPollard, Katherine, S.1 aGoodwin, Kelly, D.1 aJansson, Janet, K.1 aGilbert, Jack, A.1 aKnight, Rob1 aConsortium, The, Earth Micr uhttps://www.nature.com/articles/nature2462100851nas a2200265 4500008004100000245009100041210006900132260001200201300001400213490000600227100002500233700002200258700001700280700002100297700002400318700001700342700002400359700002100383700002000404700003300424700003100457700002300488700002000511856005400531 2017 eng d00aDecadal ecosystem response to an anomalous melt season in a polar desert in Antarctica0 aDecadal ecosystem response to an anomalous melt season in a pola c09/2017 a1334-13380 v11 aGooseff, Michael, N.1 aBarrett, John, E.1 aAdams, Byron1 aDoran, Peter, T.1 aFountain, Andrew, G1 aLyons, Berry1 aMcKnight, Diane, M.1 aPriscu, John, C.1 aSokol, Eric, R.1 aTakacs-Vesbach, Cristina, D.1 aVandegehuchte, Martijn, L.1 aVirginia, Ross, A.1 aWall, Diana, H. uhttps://www.nature.com/articles/s41559-017-0253-003366nas a2200205 4500008004100000245009200041210006900133260001200202300001400214490000700228520274200235100001902977700001802996700001903014700001503033700001903048700002303067700002403090856004603114 2017 eng d00aDissolved black carbon in the global cryosphere: Concentrations and chemical signatures0 aDissolved black carbon in the global cryosphere Concentrations a c06/2017 a6226-62340 v443 a

Black carbon (BC) is derived from the incomplete combustion of biomass and fossil fuels and can enhance glacial recession when deposited on snow and ice surfaces. Here we explore the influence of environmental conditions and the proximity to anthropogenic sources on the concentration and composition of dissolved black carbon (DBC), as measured by benzenepolycaroxylic acid (BPCA) markers, across snow, lakes, and streams from the global cryosphere. Data are presented from Antarctica, the Arctic, and high alpine regions of the Himalayas, Rockies, Andes, and Alps. DBC concentrations spanned from 0.62 μg/L to 170 μg/L. The median and (2.5, 97.5) quantiles in the pristine samples were 1.8 μg/L (0.62, 12), and nonpristine samples were 21 μg/L (1.6, 170). DBC is susceptible to photodegradation when exposed to solar radiation. This process leads to a less condensed BPCA signature. In general, DBC across the data set was composed of less polycondensed DBC. However, DBC from the Greenland Ice Sheet (GRIS) had a highly condensed BPCA molecular signature. This could be due to recent deposition of BC from Canadian wildfires. Variation in DBC appears to be driven by a combination of photochemical processing and the source combustion conditions under which the DBC was formed. Overall, DBC was found to persist across the global cryosphere in both pristine and nonpristine snow and surface waters. The high concentration of DBC measured in supraglacial melt on the GRIS suggests that DBC can be mobilized across ice surfaces. This is significant because these processes may jointly exacerbate surface albedo reduction in the cryosphere. 

1 aKhan, Alia, L.1 aWagner, Sasha1 aJaffé, Rudolf1 aXian, Peng1 aWilliams, Mark1 aArmstrong, Richard1 aMcKnight, Diane, M. uhttp://doi.wiley.com/10.1002/2017GL07348502308nas a2200217 4500008004100000022001400041245010100055210006900156260001200225300001400237490000700251520161900258100002501877700001601902700002001918700002201938700002401960700001801984700002402002856006402026 2017 eng d a0722-406000aFreshwater diatom biogeography and the genus Luticola: an extreme case of endemism in Antarctica0 aFreshwater diatom biogeography and the genus Luticola an extreme c03/2017 a1185-11960 v403 a

Historical views have characterized Antarctica as a frozen desert with low diversity, although recent studies suggest that this may not be true for microscopic organisms. For microbes, assessing endemism in the Antarctic region has been particularly important, especially against a backdrop of debate regarding their presumed cosmopolitan nature. To contribute to this conversation, we highlight the observed endemism of the freshwater diatom genus Luti- cola in Antarctica by synthesizing the results of a modern high-resolution taxonomy from the Continental, Maritime, and sub-Antarctic regions. We report that Luticola has one of the highest endemic rates of any diatom genus in Antarctica, in terms of total number of species (taxon endemism) and percentage of the entire genus (phylogenetic endemism). Of the over 200 species of Luticola globally, nearly 20% (43) occur in the Antarctic, with 42 of these being endemic. Within regions, Maritime Antarctica has the largest number of Luticola species and endemics (28 and 23, respectively), followed by Continental Antarctica (14, 9) and sub-Antarctic islands (8, 6). Thus, 38 of the 42 endemics are found in a single region only. While the timing of Luticola diversi cation has not been established, fossil evidence suggests recent invasions and/or diversi cation over a relatively short geologic timescale. Understanding the origin and evolution of endemic diatom species in Antarctica will help us better understand microbial biogeography, as well as assess and interpret impacts of large-scale environmental change taking place at southern latitudes.

1 aKociolek, J. Patrick1 aKopalova, K1 aHamsher, S., E.1 aKohler, Tyler, J.1 aVan de Vijver, Bart1 aConvey, Peter1 aMcKnight, Diane, M. uhttps://link.springer.com/article/10.1007/s00300-017-2090-701634nas a2200217 4500008004100000245010200041210006900143260001200212300001400224490000600238520092200244100002401166700002901190700002201219700002101241700002501262700002401287700001301311700002101324856007101345 2017 eng d00aHigh-resolution elevation mapping of the McMurdo Dry Valleys, Antarctica, and surrounding regions0 aHighresolution elevation mapping of the McMurdo Dry Valleys Anta c07/2017 a435 - 4430 v93 a

We present detailed surface elevation measurements for the McMurdo Dry Valleys, Antarctica derived from aerial lidar surveys flown in the austral summer of 2014–2015 as part of an effort to understand geomorphic changes over the past decade. Lidar return density varied from 2 to > 10 returns  m−2 with an average of about 5  returns  m−2. Vertical and horizontal accuracies are estimated to be 7 and 3 cm, respectively. In addition to our intended targets, other ad hoc regions were also surveyed including the Pegasus flight facility and two regions on Ross Island, McMurdo Station, Scott Base (and surroundings), and the coastal margin between Cape Royds and Cape Evans. These data are included in this report and data release. The combined data are freely available at https://doi.org/10.5069/G9D50JX3.

1 aFountain, Andrew, G1 aFernandez-Diaz, Juan, C.1 aObryk, Maciek, K.1 aLevy, Joseph, S.1 aGooseff, Michael, N.1 aVan Horn, David, J.1 aMorin, P1 aShrestha, Ramesh uhttps://www.earth-syst-sci-data.net/9/435/2017/essd-9-435-2017.pdf02751nas a2200181 4500008004100000022001300041245010100054210006900155260001200224300001200236490000800248520216000256100002502416700002102441700002402462700001702486856006602503 2017 eng d a0169555X00aHydrologic connectivity and implications for ecosystem processes - Lessons from naked watersheds0 aHydrologic connectivity and implications for ecosystem processes c01/2017 a63 - 710 v2773 a

Hydrologic connectivity has received great attention recently as our conceptual models of watersheds and water quality have evolved in the past several decades. However, the structural complexity of most temperate watersheds (i.e. connections among shallow soils, deep aquifers, the atmosphere and streams) and the dynamic seasonal changes that occur within them (i.e., plant senescence which impacts evapotranspiration) create significant challenges to characterizing or quantifying hydrologic connectivity. The McMurdo Dry Valleys, a polar desert in Antarctica, provide a unique opportunity to study hydrologic connectivity because there is no vegetative cover (and therefore no transpiration), and no deep aquifers connected to surface soils or streams. Glacier melt provides stream flow to well-established channels and closed-basin, ice-covered lakes on the valley floor. Streams are also connected to shallow hyporheic zones along their lengths, which are bounded at ~75 cm depth by ice-cemented permafrost. These hydrologic features and connections provide water for and underpin biological communities. Hence, exchange of water among them provides a vector for exchange of energy and dissolved solutes. Connectivity is dynamic on timescales of a day to a flow season (6–12 weeks), as streamflow varies over these timescales. The timescales over which these connections occur is also dynamic. Exchanges between streams and hyporheic zones, for example, have been estimated to be as short as hours to as long as several weeks. These exchanges have significant implications for the biogeochemistry of these systems and the biotic communities in each feature. Here we evaluate the lessons we can learn about hydrologic connectivity in the MDV watersheds that are simplified in the context of processes occurring and water reservoirs included in the landscape, yet are sensitive to climate controls and contain substantial physical heterogeneity. We specifically explore several metrics that are simple and/or commonly employed in hydrologic analyses and interpret them in the context of connectivity between and among hydrologic features.

1 aGooseff, Michael, N.1 aWlostowski, Adam1 aMcKnight, Diane, M.1 aJaros, Chris uhttp://linkinghub.elsevier.com/retrieve/pii/S0169555X1630248302691nas a2200229 4500008004100000022001400041245015500055210006900210260001200279490000700291520189400298653003402192653001902226653002402245653002202269653002302291100002202314700001202336700002002348700002902368856006402397 2017 eng d a0722-406000aImpact of nitrogen and phosphorus on phytoplankton production and bacterial community structure in two stratified Antarctic lakes: a bioassay approach0 aImpact of nitrogen and phosphorus on phytoplankton production an c05/20170 v403 a

Arctic, Antarctic, and alpine ecosystems are recognized as sensors and sentinels of global change. As a consequence of their high sensitivity to minor climatic perturbations, permanently ice-covered lakes located in the McMurdo Dry Valleys (MDV), Antarctica, represent end members in the global network of inland bodies of water. Episodic climatic events in the form of increased summer glacial melt result in inputs of organic sediment and nutrients from glacial streams to these closed basins. Phytoplankton communities residing in the oligotrophic water columns are highly responsive to pulses in nutrient availability; however, there is a lack of understanding on whether specific phytoplankton groups are more competitive during a summer flood event and how shifts in the phytoplankton community may influence heterotrophic bacteria. A bioassay approach in 3-l bottles was used to investigate the influence of inorganic nitrogen and phosphorus availability on planktonic communities from the oligotrophic upper waters of two chemically distinct MDV lakes (Lakes Bonney and Fryxell) which differ in their external inputs and water column N/P stoichiometry. While microbial community responses varied between lakes and were nutrient-dependent, stimulation of phytoplankton biomass and productivity across all treatments was strongly linked with increased abundance of a single phytoplankton phylum (Chlorophyta). Despite stimulation of phytoplankton growth, primary and bacterial productivity was generally uncoupled; however, shifts in bacterial community diversity were observed in bioassays amended with either P or NP. We suggest that climate-associated increases in phytoplankton production and concomitant shifts in diversity will influence MDV bacterial community structure by altering the availability and composition of autochthonous carbon for heterotrophic production.

10aAlgal–bacteria interactions10aclimate change10aMcMurdo Dry Valleys10aNutrient bioassay10aPrimary production1 aTeufel, Amber, G.1 aLi, Wei1 aKiss, Andor, J.1 aMorgan-Kiss, Rachael, M. uhttps://link.springer.com/article/10.1007/s00300-016-2025-800637nas a2200157 4500008004100000245015300041210007000194260001200264300001200276490000700288100002200295700002100317700002100338700002700359856009300386 2017 eng d00aThe influence of föhn winds on Glacial Lake Washburn and palaeotemperatures in the McMurdo Dry Valleys, Antarctica, during the Last Glacial Maximum0 ainfluence of föhn winds on Glacial Lake Washburn and palaeotempe c10/2017 a457-4670 v291 aObryk, Maciek, K.1 aDoran, Peter, T.1 aWaddington, E.D.1 aMcKay, Christopher, P. uhttps://www.cambridge.org/core/product/identifier/S0954102017000062/type/journal_article02036nas a2200145 4500008004100000245014100041210006900182260001200251490000700263520148900270100002301759700002901782700001701811856006201828 2017 eng d00aMultiple ice-binding proteins of probable prokaryotic origin in an Antarctic lake alga, Chlamydomonas sp. ICE-MDV (Chlorophyceae)0 aMultiple icebinding proteins of probable prokaryotic origin in a c08/20170 v533 a

Ice‐associated algae produce ice‐binding proteins (IBPs) to prevent freezing damage. The IBPs of the three chlorophytes that have been examined so far share little similarity across species, making it likely that they were acquired by horizontal gene transfer (HGT). To clarify the importance and source of IBPs in chlorophytes, we sequenced the IBP genes of another Antarctic chlorophyte, Chlamydomonas sp. ICE‐MDV (Chlamy‐ICE). Genomic DNA and total RNA were sequenced and screened for known ice‐associated genes. Chlamy‐ICE has as many as 50 IBP isoforms, indicating that they have an important role in survival. The IBPs are of the DUF3494 type and have similar exon structures. The DUF3494 sequences are much more closely related to prokaryotic sequences than they are to sequences in other chlorophytes, and the chlorophyte IBP and ribosomal 18S phylogenies are dissimilar. The multiple IBP isoforms found in Chlamy‐ICE and other algae may allow the algae to adapt to a greater variety of ice conditions than prokaryotes, which typically have a single IBP gene. The predicted structure of the DUF3494 domain has an ice‐binding face with an orderly array of hydrophilic side chains. The results indicate that Chlamy‐ICE acquired its IBP genes by HGT in a single event. The acquisitions of IBP genes by this and other species of Antarctic algae by HGT appear to be key evolutionary events that allowed algae to extend their ranges into polar environments.

1 aRaymond, James, A.1 aMorgan-Kiss, Rachael, M.1 aValentin, K. uhttps://onlinelibrary.wiley.com/doi/abs/10.1111/jpy.1255002451nas a2200193 4500008004100000245011100041210006900152260001200221300001400233490000700247520180200254100002402056700002302080700002102103700002502124700002002149700002402169856006402193 2017 eng d00aThermal autecology describes the occurrence patterns of four benthic diatoms in McMurdo Dry Valley streams0 aThermal autecology describes the occurrence patterns of four ben c06/2017 a2381-23960 v403 a

Benthic microbial mats in the glacial-fed melt- water streams are hotspots of productivity in the McMurdo Dry Valleys (MDV), Antarctica. Benthic diatoms are common in these mats and the[45 primarily endemic taxa represent the most diverse group of eukaryotes in the MDV. In this harsh polar desert, streams are thermally dynamic with daily water temperatures varying 6–9 °C and daily maximum temperatures as high as 15 °C. Stream temperature may play a role in determining growth rates and survival strategies. To understand taxon-specific adaptations to their environment, we measured the growth rates of unialgal cultures of four diatom taxa (Psammothidium papilio, Hantzschia abundans, Hantzschia amphioxys, and Hantzschia amphioxys f. muelleri) under three temperature conditions (7.6, 10, and 15 °C) that were representative of maximum daily stream temperatures. We found that P. papilio exhibited a constant growth rate across the full temperature range; this species is most common in streams that begin to flow early in the summer and with less variable thermal regimes. Growth rates for H. abundans were greatest at 15 °C, but showed a non-linear relationship with temperature. H. amphioxys f. muelleri

grew faster than the other taxa studied and thrived at 10 °C. Hantzschia amphioxys grew only at the two lower temperatures. These results aligned with the observed relationships between each taxon’s relative abundance and stream temperatures in the long-term record maintained by the MDV Long-Term Ecological Research program. Overall, our observations suggest that differences in thermal optima may be one factor contributing to and maintaining the diversity of benthic diatom flora in the MDV.
1 aDarling, Joshua, P.1 aGarland, Deena, D.1 aStanish, Lee, F.1 aEsposito, Rhea, M.M.1 aSokol, Eric, R.1 aMcKnight, Diane, M. uhttps://link.springer.com/article/10.1007/s00300-017-2151-y01407nas a2200445 4500008004100000245007700041210006900118260001200187490005900199100002200258700002400280700001900304700002100323700002100344700001900365700002400384700002000408700001700428700002400445700001700469700002300486700001900509700002100528700002000549700002100569700002200590700002300612700002500635700001800660700002200678700002300700700002300723700002500746700001800771700002000789700002400809700002600833700002500859856007700884 2016 eng d00aAerobiology Over Antarctica – A New Initiative for Atmospheric Ecology0 aAerobiology Over Antarctica A New Initiative for Atmospheric Eco c02/20160 v7767961946103149272350113651344451428464791101239365741 aPearce, David, A.1 aAlekhina, Irina, A.1 aTerauds, Aleks1 aWilmotte, Annick1 aQuesada, Antonio1 aEdwards, Arwyn1 aDommergue, Aurelien1 aSattler, Birgit1 aAdams, Byron1 aMagalhaes, Catarina1 aChu, Wan-Loy1 aLau, Maggie, C. Y.1 aCary, Craig, S1 aSmith, David, J.1 aWall, Diana, H.1 aEguren, Gabriela1 aMatcher, Gwynneth1 aBradley, James, A.1 ade Vera, Jean-Pierre1 aElster, Josef1 aHughes, Kevin, A.1 aCuthbertson, Lewis1 aBenning, Liane, G.1 aGunde-Cimerman, Nina1 aConvey, Peter1 aHong, Soon, Gyu1 aPointing, Steve, B.1 aPellizari, Vivian, H.1 aVincent, Warwick, F. uhttp://journal.frontiersin.org/Article/10.3389/fmicb.2016.00016/abstract01022nas a2200289 4500008004100000245010700041210006900148260001200217300001400229490000700243100002800250700002200278700002500300700001900325700002000344700002700364700002500391700002600416700002300442700001700465700001900482700002000501700002100521700001800542700002100560856015100581 2016 eng d00aBiogeochemistry and microbial diversity in the marine cavity beneath the McMurdo Ice Shelf, Antarctica0 aBiogeochemistry and microbial diversity in the marine cavity ben c11/2015 a572 - 5860 v611 aVick-Majors, Trista, J.1 aAchberger, Amanda1 aSantibáñez, Pamela1 aDore, John, E.1 aHodson, Timothy1 aMichaud, Alexander, B.1 aChristner, Brent, C.1 aAi, Jill., Mikucki Ji1 aSkidmore, Mark, L.1 aPowell, Ross1 aAdkins, Peyton1 aBarbante, Carlo1 aMitchell, Andrew1 aScherer, Reed1 aPriscu, John, C. uhttp://doi.wiley.com/10.1002/lno.v61.2http://doi.wiley.com/10.1002/lno.10234http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flno.1023401706nas a2200145 4500008004100000245009500041210006900136260001200205520122100217100001901438700001901457700001401476700002401490856004601514 2016 eng d00aDissolved black carbon in Antarctic lakes: chemical signatures of past and present sources0 aDissolved black carbon in Antarctic lakes chemical signatures of c06/20163 a

The perennially ice-covered, closed-basin lakes in the McMurdo Dry Valleys, Antarctica, serve as sentinels for understanding the fate of dissolved black carbon from glacial sources in aquatic ecosystems. Here we show that dissolved black carbon can persist in freshwater and saline surface waters for thousands of years, while preserving the chemical signature of the original source materials. The ancient brines of the lake bottom waters have retained dissolved black carbon with a woody chemical signature, representing long-range transport of black carbon from wildfires. In contrast, the surface waters are enriched in contemporary black carbon from fossil fuel combustion. Comparison of samples collected 25 years apart from the same lake suggests that the enrichment in anthropogenic black carbon is recent. Differences in the chemical composition of dissolved black carbon among the lakes are likely due to biogeochemical processing such as photochemical degradation and sorption on metal oxides.

1 aKhan, Alia, L.1 aJaffé, Rudolf1 aDing, Yan1 aMcKnight, Diane, M. uhttp://doi.wiley.com/10.1002/2016GL06860900939nas a2200217 4500008004100000022001400041245011900055210006900174260001200243100001600255700002000271700002200291700002100313700002500334700002000359700002400379700001700403700002200420700002400442856025500466 2016 eng d a0722-406000aEvidence for dispersal and habitat controls on pond diatom communities from the McMurdo Sound Region of Antarctica0 aEvidence for dispersal and habitat controls on pond diatom commu c02/20161 aSakaeva, A.1 aSokol, Eric, R.1 aKohler, Tyler, J.1 aStanish, Lee, F.1 aSpaulding, Sarah, A.1 aHowkins, Adrian1 aWelch, Kathleen, A.1 aLyons, Berry1 aBarrett, John, E.1 aMcKnight, Diane, M. uhttp://link.springer.com/10.1007/s00300-016-1901-6http://link.springer.com/content/pdf/10.1007/s00300-016-1901-6http://link.springer.com/content/pdf/10.1007/s00300-016-1901-6.pdfhttp://link.springer.com/article/10.1007/s00300-016-1901-6/fulltext.html00639nas a2200205 4500008004100000245007600041210006900117260001200186300001300198490000700211100001700218700002500235700001700260700002400277700001700301700002500318700002100343700001700364856005200381 2016 eng d00aHydrological Controls on Ecosystem Dynamics in Lake Fryxell, Antarctica0 aHydrological Controls on Ecosystem Dynamics in Lake Fryxell Anta c07/2016 ae01590380 v111 aHerbei, Radu1 aRytel, Alexander, L.1 aLyons, Berry1 aMcKnight, Diane, M.1 aJaros, Chris1 aGooseff, Michael, N.1 aPriscu, John, C.1 aHewitt, Judi uhttp://dx.plos.org/10.1371/journal.pone.015903804197nas a2200253 4500008004100000245012900041210006900170260003900239490001000278520331600288653001503604653002503619653002903644653002903673653002103702653001903723653002403742653002303766653001703789653002303806100002203829700002903851856006303880 2016 eng d00aInfluence of abiotic drivers (light and nutrients) on photobiology and diversity of Antarctic lake phytoplankton communities0 aInfluence of abiotic drivers light and nutrients on photobiology aOxford, OHbMiami Universityc20160 vPh.D.3 a

Arctic, Antarctic, and alpine ecosystems are recognized as sensors and sentinels of global climate change. As a consequence of their high sensitivity to minor climatic perturbations, permanently ice-covered lakes located in the McMurdo Dry Valleys (MCM), Antarctica, represent end members in the global network of inland bodies of water. Episodic climatic events in the form of increased summer glacial melt result in inputs of organic sediment and nutrients from glacial streams to these closed basins. By better understanding how Antarctic lake communities respond to mimicked climate change, we can more accurately predict how they will react to further temperature changes in the future. We began by investigating the influence of inorganic nitrogen and phosphorus availability on planktonic communities residing in the oligotrophic upper waters of two chemically distinct MCM lakes (Lakes Bonney and Fryxell) which differ in their external inputs as well as water column N:P stoichiometry. Although microbial community responses varied between the lakes and were nutrient-dependent, stimulation of phytoplankton biomass and productivity across all treatments was strongly linked with increased abundance of a single phytoplankton phylum (Chlorophyta). Despite stimulation of phytoplankton growth, primary and bacterial productivity were largely uncoupled across all enrichments. We suggest that climate-associated shifts in phytoplankton diversity influence the bacterial community structure by altering the availability and composition of autochthonous carbon for heterotrophic production. To monitor the physiological adaptations that occur over time and depth, we then transplanted two dominant phytoplankton, Chlamydomonas sp. ICE- MDV and Isochrysis sp. MDV back into the Lake Bonney water column. Our results demonstrated that both organisms are specialists for surviving specific depths of the water column and are capable of acclimating to their native environment within a short period of time, and that the chlorophyte Chlamydomonas sp. ICE-MDV most likely makes this adjustment via photoacclimation and accumulating chlorophyll-a per cell. The final study presented here investigated whether or not the dominant chlorophyte, Chlamydomonas sp. ICE-MDV has retained the ability to respond to a diel 12-hour day/night cycle. Although light levels in MCM lakes remain low during the austral summers, daily irradiation varies by as much as tenfold during the course of the day, resulting in a circadian-like light cycle for organisms residing there. With decreased ice coverage on the lakes due to climate change and increased melt, it is likely that these light variations will become amplified over time. This study tested for the presence of a circadian rhythm under various light quality, light quantity, and temperature conditions and demonstrated that although a diel rhythm was maintained in terms of growth and several photochemical parameters, a true circadian rhythm was not identified. Although it is predicted that photosynthetic communities in polar regions will be more responsive to climate warming and episodic events, the complexity of these systems provides numerous challenges to understanding how these organism will adapt in the future.

10aAntarctica10abacterial production10aChlamydomonas sp ICE MDV10aChlorophyll fluorescence10acircadian rhythm10aclimate change10aMcMurdo Dry Valleys10anutrient amendment10aphotobiology10aPrimary production1 aTeufel, Amber, G.1 aMorgan-Kiss, Rachael, M. uhttp://rave.ohiolink.edu/etdc/view?acc_num=miami146841156403056nas a2200133 4500008004100000245014700041210006900188260003900257490001000296520251200306100001202818700002902830856006302859 2016 eng d00aInfluence of environmental drivers and interactions on the microbial community structures in permanently stratified meromictic Antarctic lakes0 aInfluence of environmental drivers and interactions on the micro aOxford, OHbMiami Universityc20160 vPh.D.3 a

The microbial loop plays important roles in the cycling of energy, carbon and elements in aquatic ecosystems. Viruses, bacteria, Archaea and microbial eukaryotes are key players in global carbon cycle and biogeochemical cycles. Investigating microbial diversity and community structure is crucial first step for understanding the ecological functioning in aquatic environment. Meromictic lakes are bodies of water and exhibit permanent stratification of major physical and chemical environmental factors. Microbial consortia residing in permanently stratified lakes exhibit relatively constant spatial stratification throughout the water column and are adapted to vastly different habitats within the same water. Pristine perennially-ice-covered lakes (Lake Bonney, Lake Fryxell and Lake Vanda) are meromictic lakes located in the McMurdo Dry Valleys (MDV) of Southern Victoria Land, Antarctica. The lakes have isolated water bodies and extremely stable strata that vary physically, chemically, and biologically within and between the water columns. The unique characteristics support microbially dominated food webs in these lakes.

In the research presented here, we gathered new understanding of how environmental drivers influence microbial community structure in these aquatic ecosystems. We explored the lake microbial ecology from three major approaches: 1). Assess trophic activities in the natural environment and identify potential environmental drivers impacting heterotrophic (β Glucosaminidase) and autotrophic (Ribulose 1,5 bisphosphate carboxylase) enzyme activities; 2). Resolve the protist community composition (i.e. autotrophic, heterotrophic and mixotrophic groups) based on high throughput sequencing and bioinformatics. Identify how the community structures correlate with specific environmental and biological factors; 3). Reveal the diversity of potential microbial interactions between the microorganisms in the MDV lakes at individual cell level, and investigate how the interactions vary between organisms with different nutritional strategies.

Studies of polar microbial communities on the cusp of environmental change will be important for predicting how microbial communities in low latitude aquatic systems will respond. This study expands the understanding of how environmental drivers interact with microbial communities in the Antarctica lakes, and provide new information to predict how the community structure will alter as response to climate changes.

1 aLi, Wei1 aMorgan-Kiss, Rachael, M. uhttp://rave.ohiolink.edu/etdc/view?acc_num=miami146975731600845nas a2200193 4500008004100000022001400041245014300055210006900198260001600267300001400283490000700297100002100304700002800325700002900353700003300382700002200415700002100437856019300458 2016 eng d a0006-356800aMicrobial Community Dynamics in Two Polar Extremes: The Lakes of the McMurdo Dry Valleys and the West Antarctic Peninsula Marine Ecosystem0 aMicrobial Community Dynamics in Two Polar Extremes The Lakes of  cJan-10-2016 a829 - 8470 v661 aBowman, Jeff, S.1 aVick-Majors, Trista, J.1 aMorgan-Kiss, Rachael, M.1 aTakacs-Vesbach, Cristina, D.1 aDucklow, Hugh, W.1 aPriscu, John, C. uhttps://academic.oup.com/bioscience/article-lookup/doi/10.1093/biosci/biw103https://academic.oup.com/bioscience/article/66/10/829/2236137/Microbial-Community-Dynamics-in-Two-Polar-Extremes00604nas a2200169 4500008004100000245007900041210006900120260001600189490000600205100002200211700002500233700002700258700002100285700002300306700002800329856007700357 2016 eng d00aMicrobial Community Structure of Subglacial Lake Whillans, West Antarctica0 aMicrobial Community Structure of Subglacial Lake Whillans West A cOct-09-20170 v71 aAchberger, Amanda1 aChristner, Brent, C.1 aMichaud, Alexander, B.1 aPriscu, John, C.1 aSkidmore, Mark, L.1 aVick-Majors, Trista, J. uhttp://journal.frontiersin.org/Article/10.3389/fmicb.2016.01457/abstract00761nas a2200241 4500008004100000022001400041245009900055210006900154260001200223300001400235490000700249100002300256700001500279700002200294700001800316700002100334700002100355700002400376700001900400700002700419700001800446856005500464 2016 eng d a0099-224000aMicrobial Mat Communities along an Oxygen Gradient in a Perennially Ice-Covered Antarctic Lake0 aMicrobial Mat Communities along an Oxygen Gradient in a Perennia c01/2016 a620 - 6300 v821 aJungblut, Anne, D.1 aHawes, Ian1 aMackey, Tyler, J.1 aKrusor, Megan1 aDoran, Peter, T.1 aSumner, Dawn, Y.1 aEisen, Jonathan, A.1 aHillman, Colin1 aGoroncy, Alexander, K.1 aStams, A., J. uhttp://aem.asm.org/lookup/doi/10.1128/AEM.02699-1502725nas a2200133 4500008004100000245007400041210006900115260004200184490001000226520224900236100002702485700002102512856005802533 2016 eng d00aMicrobially mediated biogeochemical cycles in polar ice covered lakes0 aMicrobially mediated biogeochemical cycles in polar ice covered  aBozeman, MTbMontana State University0 vPh.D.3 a

Lakes are important sites for globally-relevant biogeochemical cycles mediated by microorganisms. In the Arctic, seasonally ice covered thermokarst lakes are a large component in Earth's carbon cycle due to their methane emissions from organic carbon degradation. In the Antarctic, over 400 unexplored lakes exist beneath the Antarctic ice sheet with unknown biogeochemical contributions to the Earth system. This dissertation seeks to investigate the biogeochemical role of microorganisms in the lake habitat and how they interact with the seasonal and permanent ice covers of lakes in polar environments. Microbiologically clean hot water drilling was used to access a subglacial lake beneath Antarctica's ice to collect, for the first time, intact sediment and water samples. Laboratory experiments on Arctic and Antarctic, seasonally and perennially, respectively, ice covered lakes were used to investigate the impact of lake ice freezing regimes on microorganisms. My results show that subglacial lake sediments beneath the West Antarctic Ice Sheet contain solute ratios that suggest relict marine sediments were deposited during previous interglacial periods. Microbial activity overprints the marine geochemical signature to produce fluxes of ions into the Subglacial Lake Whillans water column, which ultimately drains to the Southern Ocean. Microbial activity in Subglacial Lake Whillans is partially fueled by biologically-formed methane diffusing from below our deepest collected (~38 cm) subglacial sediment samples. The ice above Subglacial Lake Whillans appears to be an important source of molecular oxygen for microorganisms to drive oxidative physiologies. My experimental evidence shows microorganisms incorporate into lake ice cover to, potentially, avoid increasing stressors from progressive lake ice freezing. Taken together, the results from this dissertation reinforce the hypothesis that subglacial environments beneath the Antarctic ice sheet are habitats for life. Further, the microorganisms in subglacial lakes participate in globally-relevant biogeochemical cycles. Here, I extend the extent of the biosphere and show sediments at the base of ice sheets are an active component of the Earth system.

1 aMichaud, Alexander, B.1 aPriscu, John, C. uhttps://scholarworks.montana.edu/xmlui/handle/1/1379302208nas a2200181 4500008004100000022001400041245010400055210006900159260001200228300001100240520160100251100002201852700002101874700001801895700002701913700002101940856006501961 2016 eng d a0022-143000aModeling the thickness of perennial ice covers on stratified lakes of the Taylor Valley, Antarctica0 aModeling the thickness of perennial ice covers on stratified lak c06/2016 a1 - 103 a
A 1-D ice cover model was developed to predict and constrain drivers of long-term ice thickness trends in chemically stratified lakes of Taylor Valley, Antarctica. The model is driven by surface radiative heat fluxes and heat fluxes from the underlying water column. The model successfully reproduced 16 a (between 1996 and 2012) of ice thickness changes for the west lobe of Lake Bonney (average ice thickness = 3.53 m) and Lake Fryxell (average ice thickness = 4.22 m). Long-term ice thickness trends require coupling with the thermal structure of the water column. The heat stored within the temperature maximum of lakes exceeding a liquid water column depth of 20 m can either impede or facilitate ice thickness change depending on the predominant climatic trend (cooling or warming). As such, shallow (<20 m deep water columns) perennially ice-covered lakes without deep temperature maxima are more sensitive indicators of climate change. The long-term ice thickness trends are a result of surface energy flux and heat flux from the deep temperature maximum in the water column, the latter of which results from absorbed solar radiation.

 

1 aObryk, Maciek, K.1 aDoran, Peter, T.1 aHicks, J., A.1 aMcKay, Christopher, P.1 aPriscu, John, C. uhttp://www.journals.cambridge.org/abstract_S002214301600069102591nas a2200193 4500008004100000245013100041210006900172260001200241300001100253490000700264520191200271100002202183700002402205700002402229700003302253700002402286700001702310856007002327 2016 eng d00aNutrient treatments alter microbial mat colonization in two glacial meltwater streams from the McMurdo Dry Valleys, Antarctica0 aNutrient treatments alter microbial mat colonization in two glac c03/2016 afiw0490 v923 a

Microbial mats are abundant in many alpine and polar aquatic ecosystems. With warmer temperatures, new hydrologic pathways are developing in these regions and increasing dissolved nutrient fluxes. In the McMurdo Dry Valleys, thermokarsting may release both nutrients and sediment, and has the potential to influence mats in glacial meltwater streams. To test the role of nutrient inputs on community structure, we created nutrient diffusing substrata (NDS) with agar enriched in N, P and N + P, with controls, and deployed them into two Dry Valley streams. We found N amendments (N and N + P) to have greater chlorophyll-a concentrations, total algal biovolume, more fine filamentous cyanobacteria and a higher proportion of live diatoms than other treatments. Furthermore, N treatments were substantially elevated in Bacteroidetes and the small diatom, Fistulifera pelliculosa. On the other hand, species richness was almost double in P and N + P treatments over others, and coccoid green algae and Proteobacteria were more abundant in both streams. Collectively, these data suggest that nutrients have the potential to stimulate growth and alter community structure in glacial meltwater stream microbial mats, and the recent erosion of permafrost and accelerated glacial melt will likely impact resident biota in polar lotic systems here and elsewhere.

1 aKohler, Tyler, J.1 aVan Horn, David, J.1 aDarling, Joshua, P.1 aTakacs-Vesbach, Cristina, D.1 aMcKnight, Diane, M.1 aWagner, Dirk uhttp://femsec.oxfordjournals.org/lookup/doi/10.1093/femsec/fiw04902226nas a2200229 4500008004100000245011100041210006900152260001200221300001100233490000700244520146700251100002401718700002201742700002301764700001901787700002201806700002401828700002101852700002001873700003301893856007001926 2016 eng d00aPatterns of bacterial biodiversity in the glacial meltwater streams of the McMurdo Dry Valleys, Antarctica0 aPatterns of bacterial biodiversity in the glacial meltwater stre c08/2016 afiw1480 v923 a

Microbial consortia dominate glacial meltwater streams from polar regions, including the McMurdo Dry Valleys (MDV), where they thrive under physiologically stressful conditions. In this study, we examined microbial mat types and sediments found in 12 hydrologically diverse streams to describe the community diversity and composition within and across sites. Sequencing of the 16S rRNA gene from 129 samples revealed ∼24 000 operational taxonomic units (<97% DNA similarity), making streams the most biodiverse habitat in the MDV. Principal coordinate analyses revealed significant but weak clustering by mat type across all streams (ANOSIM R-statistic = 0.28) but stronger clustering within streams (ANOSIM R-statistic from 0.28 to 0.94). Significant relationships (P < 0.05) were found between bacterial diversity and mat ash-free dry mass, suggesting that diversity is related to the hydrologic regimes of the various streams, which are predictive of mat biomass. However, correlations between stream chemistry and community members were weak, possibly reflecting the importance of internal processes and hydrologic conditions. Collectively, these results suggest that localized conditions dictate bacterial community composition of the same mat types and sediments from different streams, and while MDV streams are hotspots of biodiversity in an otherwise depauperate landscape, controls on community structure are complex and site specific.

1 aVan Horn, David, J.1 aWolf, Caitlin, R.1 aColman, Daniel, R.1 aJiang, Xiaoben1 aKohler, Tyler, J.1 aMcKnight, Diane, M.1 aStanish, Lee, F.1 aYazzie, Terrill1 aTakacs-Vesbach, Cristina, D. uhttp://femsec.oxfordjournals.org/lookup/doi/10.1093/femsec/fiw14802242nas a2200181 4500008004100000245012300041210006900164260001200233300001400245490000700259520163300266100002101899700002501920700002401945700001701969700001701986856005702003 2016 eng d00aPatterns of hydrologic connectivity in the McMurdo dry valleys, Antarctica: a synthesis of 20 years of hydrologic data0 aPatterns of hydrologic connectivity in the McMurdo dry valleys A c04/2016 a2958-29750 v303 a

Streams in the McMurdo Dry Valleys (MDVs) of Antarctica moderate an important hydrologic and biogeochemical connection between upland alpine glaciers, valley-bottom soils, and lowland closed-basin lakes. Moreover, MDV streams are simple but dynamic systems ideal for studying interacting hydrologic and ecological dynamics. This work synthesizes 20 years of hydrologic data, collected as part of the MDVs Long-Term Ecological Research project, to assess spatial and temporal dynamics of hydrologic connectivity between glaciers, streams, and lakes. Long-term records of stream discharge (Q), specific electrical conductance (EC), and water temperature (T) from 18 streams were analysed in order to quantify the magnitude, duration, and frequency of hydrologic connections over daily, annual, and inter-annual timescales. At a daily timescale, we observe predictable diurnal variations in Q, EC, and T. At an annual timescale, we observe longer streams to be more intermittent, warmer, and have higher median EC values, compared to shorter streams. Longer streams also behave chemostatically with respect to EC, whereas shorter streams are more strongly characterized by dilution. Inter-annually, we observe significant variability in annual runoff volumes, likely because of climatic variability over the 20 record years considered. Hydrologic connections at all timescales are vital to stream ecosystem structure and function. This synthesis of hydrologic connectivity in the MDVs provides a useful end-member template for assessing hydrologic connectivity in more structurally complex temperate watersheds. 

1 aWlostowski, Adam1 aGooseff, Michael, N.1 aMcKnight, Diane, M.1 aJaros, Chris1 aLyons, Berry uhttp://onlinelibrary.wiley.com/doi/10.1002/hyp.1081804082nas a2200157 4500008004100000245010000041210006900141260001200210490000700222520353000229100002903759700002503788700001803813700002103831856007203852 2016 eng d00aPhotoadaptation to the polar night by phytoplankton in a permanently ice-covered Antarctic lake0 aPhotoadaptation to the polar night by phytoplankton in a permane c05/20150 v613 a

Photosynthetic microorganisms are a primary source of new organic carbon production in polar ecosystems. Despite their importance, relatively little is known about how they adapt to the bimodal solar cycles that exist at high latitudes. To understand how phytoplankton adapt to the extreme seasonal change in photoperiod, we transplanted cultures of a well-studied laboratory model for photosynthetic cold adaptation, Chlamydomonas raudensis UWO241, back to the water column of Lake Bonney (McMurdo Dry Valleys, Antarctica) at the depth from which it was originally cultured. The organism was suspended at this depth in dialysis tubing to allow the microalga to respond to the in situ light, temperature and dissolved ions. We then integrated in situ biological and chemical measurements with environmental molecular analyses and compared the responses of transplanted C. raudensis cultures with the natural phytoplankton community over the 6-week transition from Antarctic summer (24-h daylight) to polar night (24-h darkness). As solar radiation declined, natural communities exhibited a cessation of inorganic carbon fixation which was accompanied by a downregulation of expression of genes encoding for essential carbon fixation and photochemistry proteins. Transplanted C. raudensis cultures matched natural community trends in the regulation of photochemistry and carbon fixation gene expression, and shifted photochemical function to a shade adapted state in response to the polar night transition. We present a conceptual model for seasonal shifts in microbial community energy and carbon acquisition which integrates past cultivation-based studies in this model photopsychrophile with a body of recent work on adaptation of natural populations to polar night.

1 aMorgan-Kiss, Rachael, M.1 aLizotte, Michael, P.1 aKong, Weidong1 aPriscu, John, C. uhttps://aslopubs.onlinelibrary.wiley.com/doi/full/10.1002/lno.1010700836nas a2200229 4500008004100000245007200041210006900113260001600182490000600198100002800204700002100232700002200253700002500275700001900300700002700319700002200346700002400368700002300392700002100415710002900436856014100465 2016 eng d00aPhysiological Ecology of Microorganisms in Subglacial Lake Whillans0 aPhysiological Ecology of Microorganisms in Subglacial Lake Whill cMar-10-20180 v71 aVick-Majors, Trista, J.1 aMitchell, Andrew1 aAchberger, Amanda1 aChristner, Brent, C.1 aDore, John, E.1 aMichaud, Alexander, B.1 aMikucki, Jill, A.1 aPurcell, Alicia, M.1 aSkidmore, Mark, L.1 aPriscu, John, C.1 aThe WISSARD Science Team uhttp://journal.frontiersin.org/article/10.3389/fmicb.2016.01705/fullhttp://journal.frontiersin.org/article/10.3389/fmicb.2016.01705/full04020nas a2200241 4500008004100000245019000041210006900231260004000300490001000340520313500350653002103485653001703506653001503523653001903538653001703557653003803574653002903612653001803641653000903659100001903668700002403687856006703711 2016 eng d00aQuantifying sources, distribution, and processing of light absorbing aerosols in the cryosphere: A comparison of dissolved and refractory black carbon in polar and high mountain regions0 aQuantifying sources distribution and processing of light absorbi aBoulder, CObUniversity of Colorado0 vPh.D.3 a

Light absorbing aerosols (LAAs) in snow and ice are one of the least understood parameters in global climate models due to complicated physical processes within the cryosphere and too few in situ observations. Ground observations are limited due to the difficulty of collecting and preserving samples for analysis from remote environments.
In order to help build a larger repository of ground observations, this dissertation explores the concentration and composition of black carbon (BC) in snow and glacial melt-water across the polar regions in the Arctic and Antarctic, as well as major mountain regions such as the Himalayas, Rockies, and Andes Mountains.

Three state-of-the-art methods for BC detection are applied in this dissertation. The first chapter identifies chemical signatures of past and present sources of dissolved black carbon (DBC) in Antarctic lakes, utilizing a DBC molecular marker method. Here we find that DBC with a woody signature is preserved in the deep, ancient brines of Antarctic lake bottom waters. In contrast, the surface waters are enriched in BC from fossil fuels. The second chapter, which also utilizes the DBC molecular marker

iii 

 

technique, explores DBC across the cryosphere. We show that DBC concentrations are surprisingly high in the bottom waters of Antarctic lakes compared to other remote regions of the cryosphere, even those located near point sources. Aged snow also contains higher DBC concentrations than fresh snow suggesting that dry deposition brings the majority of BC to the cryosphere. Additionally, the DBC composition across samples from the cryosphere are similar due to high amounts of solar exposure leading to photodegradation, except in fresh snow with a wildfire signature. The third and fourth chapters utilize the Single Particle Soot Photometer to measure refractory black carbon (rBC). The third chapter also applies spectral albedo measurements and the light absorption heating method to find that coal dust from an active mine in Svalbard, Norway significantly reduces the spectral reflectance of the surrounding Arctic surface snow. The fourth chapter reports aerosol rBC concentrations in the boundary layer of the McMurdo Dry Valleys, as well as in snow from the accumulation area of the Commonwealth Glacier. Here we determine that aerosol concentrations increase during high wind events, but there is no significant trend in deposition in the snow pit. This could be due to sporadic deposition during katabatic wind events.

These findings support the importance of real in-situ observations in order to fully understand the role of BC in the global carbon cycle. It is also evident that local environmental processes can control the concentrations and composition of BC in the cryosphere. These ground-based measurements will likely serve as ground validation for future remote sensing of snow/ice impurities and LAAs deposition models.

 

10aapplied sciences10ablack carbon10acryosphere10aearth sciences10aglacial melt10ahealth and environmental sciences10alight absorbing aerosols10apolar regions10asnow1 aKhan, Alia, L.1 aMcKnight, Diane, M. uhttps://search.proquest.com/docview/1834518541?accountid=1450300943nas a2200253 4500008004100000022001400041245008700055210006900142260001600211300001400227490000700241100002200248700002100270700002600291700002500317700001200342700002900354700002100383700002100404700002800425700002700453700002200480856018700502 2016 eng d a0006-356800aResponses of Antarctic Marine and Freshwater Ecosystems to Changing Ice Conditions0 aResponses of Antarctic Marine and Freshwater Ecosystems to Chang cJan-10-2016 a864 - 8790 v661 aObryk, Maciek, K.1 aDoran, Peter, T.1 aFriedlaender, Ari, S.1 aGooseff, Michael, N.1 aLi, Wei1 aMorgan-Kiss, Rachael, M.1 aPriscu, John, C.1 aSchofield, Oscar1 aStammerjohn, Sharon, E.1 aSteinberg, Deborah, K.1 aDucklow, Hugh, W. uhttps://academic.oup.com/bioscience/article-lookup/doi/10.1093/biosci/biw109https://academic.oup.com/bioscience/article/66/10/864/2415532/Responses-of-Antarctic-Marine-and-Freshwater01836nas a2200193 4500008004100000245013000041210006900171260001200240300001600252490000700268520116600275100002501441700002401466700002001490700002401510700002401534700001701558856006701575 2016 eng d00aStream biogeochemical and suspended sediment responses to permafrost degradation in stream banks in Taylor Valley, Antarctica0 aStream biogeochemical and suspended sediment responses to permaf c03/2016 a1723 - 17320 v133 a

 Stream channels in the McMurdo Dry Valleys are characteristically wide, incised, and stable. At typical flows, streams occupy a fraction of the oversized channels, providing habitat for algal mats. In January 2012, we discovered substantial channel erosion and subsurface thermomechanical erosion undercutting banks of the Crescent Stream. We sampled stream water along the impacted reach and compared concentrations of solutes to the long-term data from this stream ( ∼  20 years of monitoring). Thermokarst-impacted stream water demonstrated higher electrical conductivity, and concentrations of chloride, sulfate, sodium, and nitrate than the long-term medians. These results suggest that this mode of lateral permafrost degradation may substantially impact stream solute loads and potentially fertilize stream and lake ecosystems. The potential for sediment to scour or bury stream algal mats is yet to be determined, though it may offset impacts of associated increased nutrient loads to streams.

1 aGooseff, Michael, N.1 aVan Horn, David, J.1 aSudman, Zachary1 aMcKnight, Diane, M.1 aWelch, Kathleen, A.1 aLyons, Berry uhttp://www.biogeosciences.net/13/1723/2016/bg-13-1723-2016.pdf04687nas a2200181 4500008004100000022001400041245015900055210006900214260001200283300001600295490000700311520405500318100001204373700001804385700002904403700001804432856005504450 2016 eng d a0099-224000aUltrastructural and Single-Cell-Level Characterization Reveals Metabolic Versatility in a Microbial Eukaryote Community from an Ice-Covered Antarctic Lake0 aUltrastructural and SingleCellLevel Characterization Reveals Met c06/2016 a3659 - 36700 v823 a

The McMurdo Dry Valleys (MCM) of southern Victoria Land, Antarctica, harbor numerous ice-covered bodies of water that provide year-round liquid water oases for isolated food webs dominated by the microbial loop. Single-cell microbial eukaryotes (protists) occupy major trophic positions within this truncated food web, ranging from primary producers (e.g., chlorophytes, haptophytes, and cryptophytes) to tertiary predators (e.g., ciliates, dinoflagellates, and choanoflagellates). To advance the understanding of MCM protist ecology and the roles of MCM protists in nutrient and energy cycling, we investigated potential metabolic strategies and microbial interactions of key MCM protists isolated from a well-described lake (Lake Bonney). Fluorescence-activated cell sorting (FACS) of enrichment cultures, combined with single amplified genome/amplicon sequencing and fluorescence microscopy, revealed that MCM protists possess diverse potential metabolic capabilities and interactions. Two metabolically distinct bacterial clades (Flavobacteria and Methylobacteriaceae) were independently associated with two key MCM lake microalgae (Isochrysis and Chlamydomonas, respectively). We also report on the discovery of two heterotrophic nanoflagellates belonging to the Stramenopila supergroup, one of which lives as a parasite ofChlamydomonas, a dominate primary producer in the shallow, nutrient-poor layers of the lake.

IMPORTANCE Single-cell eukaryotes called protists play critical roles in the cycling of organic matter in aquatic environments. In the ice-covered lakes of Antarctica, protists play key roles in the aquatic food web, providing the majority of organic carbon to the rest of the food web (photosynthetic protists) and acting as the major consumers at the top of the food web (predatory protists). In this study, we utilized a combination of techniques (microscopy, cell sorting, and genomic analysis) to describe the trophic abilities of Antarctic lake protists and their potential interactions with other microbes. Our work reveals that Antarctic lake protists rely on metabolic versatility for their energy and nutrient requirements in this unique and isolated environment.

1 aLi, Wei1 aPodar, Mircea1 aMorgan-Kiss, Rachael, M.1 aKelly, R., M. uhttp://aem.asm.org/lookup/doi/10.1128/AEM.00478-1604921nas a2200181 4500008004100000022001400041245008200055210006900137260001200206300001300218520434700231100002104578700001504599700002204614700002304636700002104659856005904680 2015 eng d a0091-761300aAntarctic microbial mats: A modern analog for Archean lacustrine oxygen oases0 aAntarctic microbial mats A modern analog for Archean lacustrine  c10/2015 aG36966.13 a

The evolution of oxygenic photosynthesis was the most important geochemical event in

Earth history, causing the Great Oxidation Event (GOE) ~2.4 b.y. ago. However, evidence is mixed as to whether O2 production occurred locally as much as 2.8 b.y. ago, creating O2 oases, or initiated just prior to the GOE. The biogeochemical dynamics of possible O2 oases have been poorly constrained due to the absence of modern analogs. However, cyanobacteria in microbial mats in a perennially anoxic region of Lake Fryxell, Antarctica, create a 1–2 mm O2-containing layer in the upper mat during summer, providing the first known modern analog for formation of benthic O2 oases. In Lake Fryxell, benthic cyanobacteria are present below the oxycline in the lake. Mat photosynthesis rates were slow due to low photon flux rate (1–2 μmol m-2 s-1) under thick ice cover, but photosynthetic O2 production was sufficient to sustain up to 50 μmol O2 L-1, sandwiched between anoxic overlying water and anoxic sedi- ments. We hypothesize that Archean cyanobacteria could have similarly created O2 oases in benthic mats prior to the GOE. Analogous mats may have been at least partly responsible for geological evidence of oxidative weathering prior to the GOE, and habitats such as Lake Fryxell provide natural laboratories where the impact of benthic O2 oases on biogeochemical signatures can be investigated. 

 

1 aSumner, Dawn, Y.1 aHawes, Ian1 aMackey, Tyler, J.1 aJungblut, Anne, D.1 aDoran, Peter, T. uhttp://geology.gsapubs.org/lookup/doi/10.1130/G36966.103321nas a2200205 4500008004100000022001400041245008600055210006900141260001200210300001600222490000700238520262100245100001702866700002302883700002402906700002502930700001502955700002402970856012102994 2015 eng d a0091-761300aAntarctic streams as a potential source of iron for the Southern Ocean: Figure 1.0 aAntarctic streams as a potential source of iron for the Southern c11/2016 a1003 - 10060 v433 a

Due to iron’s role in oceanic primary production, there has been great interest in quantifying the importance of Fe in regions where concentrations are very low and macronutrients, nitrate and phosphate, are available. Measurements of filterable (i.e., <0.4 μm) Fe concentrations in streams from Taylor Valley, McMurdo Dry Valleys, Antarctica, suggest that coastal-zone stream Fe input to the Southern Ocean could potentially play an important role in primary production in nearshore regions. Filterable Fe (fFe) data from streams in the McMurdo Dry Valleys were used to represent glacier meltwater that flows through ice-free landscape with the potential of transporting Fe to the Antarctic coastal zone. Estimates of potential fFe flux to the Antarctic Peninsula region using our mean fFe concentration of 10.6 µg L–1 combined with an estimate of ice-free area for the Antarctic Peninsula result in an fFe flux of 1.2 × 107 g yr–1. Although small compared to iceberg and aeolian Fe fluxes, future stream input to the Southern Ocean could increase due to glacier retreat and 

1 aLyons, Berry1 aDailey, Kelsey, R.1 aWelch, Kathleen, A.1 aDeuerling, Kelly, M.1 aWelch, Sue1 aMcKnight, Diane, M. uhttp://geology.gsapubs.org/lookup/doi/10.1130/G36989.1http://geology.geoscienceworld.org/lookup/doi/10.1130/G36989.108594nas a2200241 4500008004100000022001300041245020300054210006900257260001200326300001400338490000800352520755800360100001907918700002407937700002007961700002407981700002308005700002308028700001708051700002708068700002708095856023008122 2015 eng d a0009254100aComparison of arsenic and molybdenum geochemistry in meromictic lakes of the McMurdo Dry Valleys, Antarctica: Implications for oxyanion-forming trace element behavior in permanently stratified lakes0 aComparison of arsenic and molybdenum geochemistry in meromictic  c05/2015 a110 - 1250 v4043 a

Water samples were collected for arsenic (As) and molybdenum (Mo) analysis from different depths in Lakes Hoare and Fryxell, both of which are located in the Taylor Valley within the McMurdo Dry Valleys of Antarctica. Sampling depths within each lake were chosen to capture variations in As and Mo concentrations and As speciation in the oxic mixolimnia and anoxic monimolimnia of these meromictic lakes. Arsenic concentrations ranged from 0.67 nmol kg− 1 to 3.54 nmol kg− 1 in Lake Hoare and from 1.69 nmol kg− 1 to 17.5 nmol kg− 1 in Lake Fryxell. Molybdenum concentrations varied between 5.05 nmol kg− 1 and 43 nmol kg− 1 in Lake Hoare, and between 3.52 nmol kg− 1 and 25.5 nmol kg− 1 in Lake Fryxell. Concentrations of As and Mo generally increased with depth in the mixolimnion of each lake, consistent with uptake near the ice–water interface by organic particles and/or Fe/Mn oxides/oxyhydroxides, followed by gravitational settling and regeneration/remineralization at depth in the vicinity of the redoxcline. Arsenic concentrations either remained constant (Hoare) or increased with depth (Fryxell) in the anoxic monimolimnia, whereas Mo exhibited dramatic decreases in concentrations across the redoxcline in both lakes. Geochemical modeling predicts that As and Mo occur as thioanions in the anoxic bottom waters of Lakes Hoare and Fryxell, and further that the contrasting behavior of both trace elements reflects the respective reactivity of their thioanions towards Fe-sulfide minerals such as mackinawite (FeS) and/or pyrite (FeS2). More specifically, the geochemical model suggests that Fe-sulfide mineral precipitation in the anoxic monimolimnia of both lakes regulates dissolved sulfide concentrations at levels that are too low for As-sulfide minerals (e.g., orpiment, realgar) to precipitate, whereas mackinawite and/or pyrite react(s) with particle reactive thiomolybdate anions, possibly forming an Fe–Mo–S mineral that precipitates and, hence, leads to Mo removal from solution.

1 aYang, Ningfang1 aWelch, Kathleen, A.1 aMohajerin, Jade1 aTelfeyan, Katherine1 aChevis, Darren, A.1 aGrimm, Deborah, A.1 aLyons, Berry1 aWhite, Christopher, D.1 aJohannesson, Karen, H. uhttp://linkinghub.elsevier.com/retrieve/pii/S0009254115001874http://api.elsevier.com/content/article/PII:S0009254115001874?httpAccept=text/xmlhttp://api.elsevier.com/content/article/PII:S0009254115001874?httpAccept=text/plain03959nas a2200205 4500008004100000022001400041245008900055210006900144260001200213300001600225490000700241520327000248100001403518700002303532700001503555700002303570700002103593700002203614856011703636 2015 eng d a0722-406000aCyanobacterial diversity in benthic mats of the McMurdo Dry Valley lakes, Antarctica0 aCyanobacterial diversity in benthic mats of the McMurdo Dry Vall c01/2015 a1097 - 11100 v383 a
Perennially ice-covered, meromictic lakes in the McMurdo Dry Valleys, Antarctica, are useful models to study the relationship between cyanobacterial and environmental variables. They have rich benthic cyanobacterial mat accumulations and stable stratification of physical and chemical conditions. Here, we evaluated cyanobacteria from benthic mats from multiple depths in three geographically separated ice-covered lakes, Lakes Vanda, Hoare and Joyce, using 16S rRNA gene clone libraries. We identified 19 ribotypes, mostly Oscillatoriales and several
Chroococcales, as well as potentially novel cyanobacterial ribotypes. The majority of ribotype diversity was shared between lakes, and only a weak relationship between ribotype community structure and environmental variables was evident. Multivariate analysis of all lake–depth combinations implied that photosynthetically active radiation, dissolved reactive phosphorus and conductivity were potentially important for shaping benthic communities in McMurdo Dry Valley lakes. Cyanobacterial-specific pigment signature analysis by high-performance liquid chromatography
showed that the cyanobacterial communities responded to light conditions similarly, irrespective of community composition. The results imply a capability within a suite of cyanobacteria to colonise, adapt and grow across broad environmental ranges and geographic space, and such adaptability may provide a high degree of community resistance and resilience to future climate-driven environmental change in Antarctic terrestrial aquatic
ecosystems.
1 aZhang, L.1 aJungblut, Anne, D.1 aHawes, Ian1 aAndersen, Dale, T.1 aSumner, Dawn, Y.1 aMackey, Tyler, J. uhttp://link.springer.com/10.1007/s00300-015-1669-0http://link.springer.com/content/pdf/10.1007/s00300-015-1669-001657nas a2200229 4500008004100000245008700041210006900128260001600197300000900213490000600222520097300228100002201201700001401223700001601237700002301253700001701276700002201293700002101315700002201336700001401358856005501372 2015 eng d00aDeep groundwater and potential subsurface habitats beneath an Antarctic dry valley0 aDeep groundwater and potential subsurface habitats beneath an An cApr-04-2017 a68310 v63 aThe occurrence of groundwater in Antarctica, particularly in the ice-free regions and along the coastal margins is poorly understood. Here we use an airborne transient electromagnetic (AEM) sensor to produce extensive imagery of resistivity beneath Taylor Valley. Regional-scale zones of low subsurface resistivity were detected that are inconsistent with the high resistivity of glacier ice or dry permafrost in this region. We interpret these results as an indication that liquid, with sufficiently high solute content, exists at temperatures well below freezing and considered within the range suitable for microbial life. These inferred brines are widespread within permafrost and extend below glaciers and lakes. One system emanates from below Taylor Glacier into Lake Bonney and a second system connects the ocean with the eastern 18 km of the valley. A connection between these two basins was not detected to the depth limitation of the AEM survey (~350 m).1 aMikucki, Jill, A.1 aAuken, E.1 aTulaczyk, S1 aVirginia, Ross, A.1 aSchamper, C.1 aSørensen, K., I.1 aDoran, Peter, T.1 aDugan, Hilary, A.1 aFoley, N. uhttp://www.nature.com/doifinder/10.1038/ncomms783100932nas a2200265 4500008004100000022001400041245013400055210006900189260001200258300001400270490000800284100002300292700002800315700001600343700002000359700002500379700002200404700002100426700001700447700002300464700002200487700002400509700001600533856011700549 2015 eng d a0029-854900aGlobal environmental change and the nature of aboveground net primary productivity responses: insights from long-term experiments0 aGlobal environmental change and the nature of aboveground net pr c04/2015 a935 - 9470 v1771 aSmith, Melinda, D.1 aLa Pierre, Kimberly, J.1 aCollins, SL1 aKnapp, Alan, K.1 aGross, Katherine, L.1 aBarrett, John, E.1 aFrey, Serita, D.1 aGough, Laura1 aMiller, Robert, J.1 aMorris, James, T.1 aRustad, Lindsey, E.1 aYarie, John uhttp://link.springer.com/10.1007/s00442-015-3230-9http://link.springer.com/content/pdf/10.1007/s00442-015-3230-902538nas a2200205 4500008004100000245010400041210006900145260001200214300001200226490000700238520189000245100002202135700002102157700001902178700001302197700001602210700001702226700002402243856006502267 2015 eng d00a Long-Term Hydrologic Control of Microbial Mat Abundance in McMurdo Dry Valley Streams, Antarctica.0 aLongTerm Hydrologic Control of Microbial Mat Abundance in McMurd c03/2015 a310-3270 v183 a

Given alterations in global hydrologic regime, we examine the role of hydrology in regulating stream microbial mat abundance in the McMurdo Dry Valleys, Antarctica. Here, perennial mats persist as a desiccated crust until revived by summer streamflow, which varies inter-annually, and has increased since the 1990s. We predicted high flows to scour mats, and intra-seasonal drying to slow growth. Responses were hypothesized to differ based on mat location within streams, along with geomorphology, which may promote (high coverage) or discourage (low coverage) accrual. We compared hydrologic trends with the biomass of green and orange mats, which grow in the channel, and black mats growing at stream margins for 16 diverse stream transects over two decades. We found mat biomass collectively decreased during first decade coinciding with low flows, and increased following elevated discharges. Green mat biomass showed the greatest correlations with hydrology and was stimulated by discharge in high coverage transects, but negatively correlated in low coverage due to habitat scour. In contrast, orange mat biomass was negatively related to flow in high coverage transects, but positively correlated in low coverage because of side-channel expansion. Black mats were weakly correlated with all hydrologic variables regardless of coverage. Lastly, model selection indicated the best combination of predictive hydrologic variables for biomass differed between mat types, but also high and low coverage transects. These results demonstrate the importance of geomorphology and species composition to modeling primary production, and will be useful in predicting ecological responses of benthic habitats to altered hydrologic regimes.

1 aKohler, Tyler, J.1 aStanish, Lee, F.1 aCrisp, Stenven1 aKoch, J.1 aLiptzin, D.1 aBaeseman, J.1 aMcKnight, Diane, M. uhttp://link.springer.com/article/10.1007%2Fs10021-014-9829-600801nas a2200253 4500008004100000022001400041245008400055210006900139260001200208300000800220490000700228100003400235700002400269700002200293700002600315700002500341700002400366700001700390700002200407700002300429700002600452700002200478856004700500 2015 eng d a1445-522600aMitochondrial DNA analyses reveal widespread tardigrade diversity in Antarctica0 aMitochondrial DNA analyses reveal widespread tardigrade diversit c12/2015 a5780 v291 aVelasco-Castrillon, Alejandro1 aMcInnes, Sandra, J.1 aSchultz, Mark, B.1 aArroniz-Crespo, Maria1 aD'Haese, Cyrille, A.1 aGibson, John, A. E.1 aAdams, Byron1 aPage, Timothy, J.1 aAustin, Andrew, D.1 aCooper, Steven, J. B.1 aStevens, Mark, I. uhttp://www.publish.csiro.au/?paper=IS1401903401nas a2200241 4500008004100000245013400041210006900175260004000244300000800284490001000292520259800302653001002900653001502910653002402925653001902949653001602968653001902984653002403003653001903027100002203046700002403068856006703092 2015 eng d00aPhysical and chemical controls on the abundance and composition of stream microbial mats from the McMurdo Dry Valleys, Antarctica0 aPhysical and chemical controls on the abundance and composition  aBoulder, CObUniversity of Colorado a2720 vPh.D.3 a

 

The McMurdo Dry Valleys of Antarctica are a cold, dry desert, yet perennial microbial mats are abundant in the ephemeral glacial meltwater streams that flow during austral summers. Three types of mats are present (orange, black, and green), and are primarily comprised of filamentous cyanobacteria, Nostoc, and chlorophytes, respectively. Mat types furthermore occupy distinct habitats within streams, utilizing the benthos, hyporheic zone, and water column, which expose them to different environmental conditions. Due to a lack of lateral inflows, allochthonous organic inputs, and negligible grazing activity, these streams are ideal for the controlled ecological study of microbial mats. Here, I investigated how mats will respond to physical disturbance, alterations in the hydrologic regime, and nutrient liberation from permafrost melt in the future. Specifically, I: 1) quantified and characterized the regrowth of mat biomass, community structure, and elemental stoichiometry after a scouring disturbance, 2) investigated how geomorphology and taxonomic identity influences the response of mat biomass to hydrologic regime in transects monitored over two decades, and 3) evaluated relationships between water chemistry and the elemental and isotopic composition of mat types over longitudinal and valley-wide gradients in Taylor Valley. I found that mats recovered ~20-50% of their biomass over the course of an austral summer following scour. Algal communities were significantly different in composition between disturbed and control treatments, but all samples naturally varied in species and elemental stoichiometry over the study period. When the long- term record of mat biomass was compared with hydrologic variables, stream channel mats (orange and green) had the greatest correlations, while marginal mats (black) showed weaker relationships with flow regime. Relationships also differed as a function of stream geomorphology, indicating the importance of substrata and gradient in conjunction with discharge. Lastly, mats showed unique elemental and isotopic compositions. Green and orange mats within the stream channel most reflected water column nutrient concentrations, while black mats showed significant nitrogen fixation. These results highlight the importance of taxonomic identity and habitat to modeling primary production here and elsewhere, and provide insight to how stream microbial mat communities are formed, maintained, and ultimately persist in an isolated polar desert.

 

10aalgae10aAntarctica10abiological sciences10aclimate change10aDisturbance10aearth sciences10aMcMurdo Dry Valleys10amicrobial mats1 aKohler, Tyler, J.1 aMcKnight, Diane, M. uhttps://search.proquest.com/docview/1690497718?accountid=1450300864nas a2200217 4500008004100000245020400041210006900245260001200314300001600326490000700342100002400349700001600373700002400389700002500413700001700438700001300455700001700468700002100485700002100506856011900527 2015 eng d00aPotential for real-time understanding of coupled hydrologic and biogeochemical processes in stream ecosystems: Future integration of telemetered data with process models for glacial meltwater streams0 aPotential for realtime understanding of coupled hydrologic and b c08/2015 a6725 - 67380 v511 aMcKnight, Diane, M.1 aCozzetto, K1 aCullis, James, D.S.1 aGooseff, Michael, N.1 aJaros, Chris1 aKoch, J.1 aLyons, Berry1 aNeupauer, R., M.1 aWlostowski, Adam uhttp://doi.wiley.com/10.1002/2015WR017618http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2F2015WR01761802527nas a2200181 4500008004100000245016700041210006900208260001200277300001600289490000700305520184500312100002102157700002402178700002402202700002102226700001702247856008102264 2015 eng d00aPressure-driven, shoreline currents in a perennially ice-covered, pro-glacial lake in Antarctica, identified from a LiCl tracer injected into a pro-glacial stream0 aPressuredriven shoreline currents in a perennially icecovered pr c05-2015 a2212 - 22310 v293 a

The distribution of streamwater within ice-covered lakes influences sub-ice currents, biological activity and shoreline morphology. Perennially ice-covered lakes in the McMurdo Dry Valleys, Antarctica, provide an excellent natural laboratory to study hydrologic–limnologic interactions under ice cover. For a 2 h period on 17 December 2012, we injected a lithium chloride tracer into Andersen Creek, a pro-glacial stream flowing into Lake Hoare. Over 4 h, we collected 182 water samples from five stream sites and 15 ice boreholes. Geochemical data showed that interflow travelled West of the stream mouth along the shoreline and did not flow towards the lake interior. The chemistry of water from Andersen Creek was similar to the chemistry of water below shoreline ice. Additional evidence for Westward flow included the morphology of channels on the ice surface, the orientation of ripple marks in lake sediments at the stream mouth and equivalent temperatures between Andersen Creek and water below shoreline ice. Streamwater deflected to the right of the mouth of the stream, in the opposite direction predicted by the Coriolis force. Deflection of interflow was probably caused by the diurnal addition of glacial runoff and stream discharge to the Eastern edge of the lake, which created a strong pressure gradient sloping to the West. This flow directed stream momentum away from the lake interior, minimizing the impact of stream momentum on sub-ice currents. It also transported dissolved nutrients and suspended sediments to the shoreline region instead of the lake interior, potentially affecting biological productivity and bedform development.

1 aCastendyk, Devin1 aMcKnight, Diane, M.1 aWelch, Kathleen, A.1 aNiebuhr, Spencer1 aJaros, Chris uhttp://doi.wiley.com/10.1002/hyp.v29.9http://doi.wiley.com/10.1002/hyp.1035202661nas a2200145 4500008004100000245006100041210006000102260004000162300000800202490000700210520218500217100002202402700002402424856006702448 2015 eng d00aQuantifying long-term geomorphology of Antarctic streams0 aQuantifying longterm geomorphology of Antarctic streams aBoulder, CObUniversity of Colorado a1850 vMS3 a

 

In 1994, 16 stream transects were established in the McMurdo Dry Valleys of Antarctica beginning a long term data set characterizing microbial communities and channel geometry. The transects were established to record microbial mat dynamics and stream geomorphology. To accomplish this, the transects were surveyed for points of interest outside and inside the stream channel. Beginning in 2010 the microbial surveys received ground based LiDAR support. This allowed for greater resolution in mapping and analyzing stream morphology than traditional surveying methods. The purpose of this study was to overlap the traditional methods of surveying individual points of interest with a data cloud representing the entire stream transect to be able to continue the microbial study into the future unabated. Using surveyed microbial mats as an indicator of a location in time, a history of channel elevations was created for 7 transects. In general, the streams have not changed significantly in the 20 year record, with exceptions being the steep channel of Bohner Stream, and Huey Creek, which receives large sediment loads from the sharply incised upstream channel, both of which saw large variations in maximum bed change exceeding 75cm and 150 cm respectively. In addition to creating an elevation history, relative bed change was plotted against the ash free dry mass of the microbial mats sampled to determine the resilience of the mats. It was found that microbial mats are more abundant in areas of near zero change. The four microbial mats studied however, which include green, black, orange, and red mats, differed greatly in adaptability with regards to bed change. Green microbial mats, which are typically hidden under large immobile rocks, were not often found in areas with any significant bed change. Conversely, orange mats were found in the most dynamic parts of the stream bed with outliers seen in areas with change exceeding 50 cm with. Finally black microbial mats had the largest values of ash free dry mass indicating the largest resilience to the scouring effects of high flow.

 

1 aCrisp, Steven, W.1 aMcKnight, Diane, M. uhttps://search.proquest.com/docview/1727444346?accountid=1450304273nas a2200193 4500008004100000022001400041245007400055210006900129260001600198300001400214490000700228520366500235100002203900700002103922700002503943700002203968700002403990856006504014 2015 eng d a0954-102000aRecovery of Antarctic stream epilithon from simulated scouring events0 aRecovery of Antarctic stream epilithon from simulated scouring e cJan-08-2015 a341 - 3540 v273 a

Microbial mats are common in polar streams and often dominate benthic biomass. Climate change may be enhancing the variability of stream flows in the Antarctic, but so far studies investigating mat responses to disturbance have been limited in this region. Mat regrowth was evaluated following disturbance by experimentally scouring rocks from an ephemeral McMurdo Dry Valley stream over two summers (2001–02 and 2012–13). Mats were sampled at the beginning and resampled at the end of the flow season. In 2012–13, mats were additionally resampled mid-season along with previously undisturbed controls. In 2001–02 rocks regained 47% of chlorophyll aand 40% of ash-free dry mass by the end of the summer, while in 2012–13 rocks regrew 18% and 27%, respectively. Mat stoichiometry differed between summers, and reflected differences in biomass and discharge. Oscillatoria spp. were greatest on scoured rocks and Phormidium spp. on undisturbed rocks. Small diatoms Humidophila andFistulifera spp. increased throughout the summer in all mats, with the latter more abundant in scoured communities. Collectively, these data suggest that mats are variable intra-annually, responsive to hydrology and require multiple summers to regrow initial biomass once lost. These results will aid the interpretation of long-term data, as well as inform Antarctic Specially Managed Area protocols.

1 aKohler, Tyler, J.1 aChatfield, Ethan1 aGooseff, Michael, N.1 aBarrett, John, E.1 aMcKnight, Diane, M. uhttp://www.journals.cambridge.org/abstract_S095410201500002404591nas a2201033 4500008004100000022001400041245009100055210006900146260001600215300001100231490000700242520199300249100002002242700002202262700001802284700001602302700002002318700001502338700001802353700001502371700001802386700001702404700001602421700001402437700001402451700001702465700001802482700002102500700001602521700001102537700001502548700002002563700001902583700001602602700001802618700001602636700001402652700001602666700001502682700001602697700001602713700001602729700001802745700001602763700001602779700001302795700001802808700001202826700001402838700001402852700001402866700001602880700001402896700001402910700002002924700001102944700001702955700001502972700002502987700001603012700001703028700001703045700001503062700001703077700001403094700001403108700001703122700001803139700001603157700002403173700002303197700001603220700001503236700001603251700001803267700001803285700001803303700001803321700001503339700001803354700002003372700001603392700001503408700001803423700001703441700001303458700002103471856006503492 2015 eng d a0954-102000aA roadmap for Antarctic and Southern Ocean science for the next two decades and beyond0 aroadmap for Antarctic and Southern Ocean science for the next tw cJan-02-2015 a3 - 180 v273 a

Antarctic and Southern Ocean science is vital to understanding natural variability, the processes that govern global change and the role of humans in the Earth and climate system. The potential for new knowledge to be gained from future Antarctic science is substantial. Therefore, the international Antarctic community came together to ‘scan the horizon’ to identify the highest priority scientific questions that researchers should aspire to answer in the next two decades and beyond. Wide consultation was a fundamental principle for the development of a collective, international view of the most important future directions in Antarctic science. From the many possibilities, the horizon scan identified 80 key scientific questions through structured debate, discussion, revision and voting. Questions were clustered into seven topics: i) Antarctic atmosphere and global connections, ii) Southern Ocean and sea ice in a warming world, iii) ice sheet and sea level, iv) the dynamic Earth, v) life on the precipice, vi) near-Earth space and beyond, and vii) human presence in Antarctica. Answering the questions identified by the horizon scan will require innovative experimental designs, novel applications of technology, invention of next-generation field and laboratory approaches, and expanded observing systems and networks. Unbiased, non-contaminating procedures will be required to retrieve the requisite air, biota, sediment, rock, ice and water samples. Sustained year-round access to Antarctica and the Southern Ocean will be essential to increase winter-time measurements. Improved models are needed that represent Antarctica and the Southern Ocean in the Earth System, and provide predictions at spatial and temporal resolutions useful for decision making. A co-ordinated portfolio of cross-disciplinary science, based on new models of international collaboration, will be essential as no scientist, programme or nation can realize these aspirations alone

1 aKennicutt, M.C.1 aChown, Steven, L.1 aCassano, J.J.1 aLiggett, D.1 aPeck, Lloyd, S.1 aMassom, R.1 aRintoul, S.R.1 aStorey, J.1 aVaughan, D.G.1 aWilson, T.J.1 aAllison, I.1 aAyton, J.1 aBadhe, R.1 aBaeseman, J.1 aBarrett, P.J.1 aBell, Elanor, R.1 aBertler, N.1 aBo, S.1 aBrandt, A.1 aBromwich, David1 aCary, Craig, S1 aClark, M.S.1 aConvey, Peter1 aCosta, E.S.1 aCowan, D.1 aDeconto, R.1 aDunbar, R.1 aElfring, C.1 aEscutia, C.1 aFrancis, J.1 aFricker, H.A.1 aFukuchi, M.1 aGilbert, N.1 aGutt, J.1 aHavermans, C.1 aHik, D.1 aHosie, G.1 aJones, C.1 aKim, Y.D.1 aLe Maho, Y.1 aLee, S.H.1 aLeppe, M.1 aLeitchenkov, G.1 aLi, X.1 aLipenkov, V.1 aLochte, K.1 aLópez-Martínez, J.1 aüdecke, C.1 aLyons, Berry1 aMarenssi, S.1 aMiller, H.1 aMorozova, P.1 aNaish, T.1 aNayak, S.1 aRavindra, R.1 aRetamales, J.1 aRicci, C.A.1 aRogan-Finnemore, M.1 aRopert-Coudert, Y.1 aSamah, A.A.1 aSanson, L.1 aScambos, T.1 aSchloss, I.R.1 aShiraishi, K.1 aSiegert, M.J.1 aSimões, J.C.1 aStorey, B.1 aSparrow, M.D.1 aWall, Diana, H.1 aWalsh, J.C.1 aWilson, G.1 aWinther, J.G.1 aXavier, J.C.1 aYang, H.1 aSutherland, W.J. uhttp://www.journals.cambridge.org/abstract_S095410201400067402282nas a2200181 4500008004100000245010600041210006900147260001200216300001200228490000800240520160500248100001301853700002801866700002901894700002101923700003001944856012601974 2014 eng d00aCiliate diversity, community structure and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica0 aCiliate diversity community structure and novel taxa in lakes of c10/2014 a175-1900 v2273 aWe report an in-depth survey of next-generation DNA sequencing of ciliate diversity and community structure in two permanently ice-covered McMurdo Dry Valley lakes during the austral summer and autumn (November 2007 and March 2008). We tested hypotheses on the relationship between species richness and environmental conditions including environmental extremes, nutrient status, and day length. On the basis of the unique environment that exists in these high-latitude lakes, we expected that novel taxa would be present. Alpha diversity analyses showed that extreme conditions-that is, high salinity, low oxygen, and extreme changes in day length-did not impact ciliate richness; however, ciliate richness was 30% higher in samples with higher dissolved organic matter. Beta diversity analyses revealed that ciliate communities clustered by dissolved oxygen, depth, and salinity, but not by season (i.e., day length). The permutational analysis of variance test indicated that depth, dissolved oxygen, and salinity had significant influences on the ciliate community for the abundance matrices of resampled data, while lake and season were not significant. This result suggests that the vertical trends in dissolved oxygen concentration and salinity may play a critical role in structuring ciliate communities. A PCR-based strategy capitalizing on divergent eukaryotic V9 hypervariable region ribosomal RNA gene targets unveiled two new genera in these lakes. A novel taxon belonging to an unknown class most closely related to Cryptocaryon irritans was also inferred from separate gene phylogenies.1 aYuan, Xu1 aVick-Majors, Trista, J.1 aMorgan-Kiss, Rachael, M.1 aPriscu, John, C.1 aAmaral-Zettler, Linda, A. uhttps://mcm.lternet.edu/content/ciliate-diversity-community-structure-and-novel-taxa-lakes-mcmurdo-dry-valleys-antarctica02195nas a2200157 4500008004100000245014100041210006900182260001200251300001000263490000700273520155900280100002401839700002101863700002401884856012901908 2014 eng d00aDiel flow pulses drive particulate organic matter transport from microbial mats in a glacial meltwater stream in the McMurdo Dry Valleys0 aDiel flow pulses drive particulate organic matter transport from c01/2014 a86-970 v503 a Many glacial meltwater streams in the McMurdo Dry Valleys (MDV) of Antarctica contain abundant microbial mats, representing hot spots of primary production in a barren landscape. These mats persist through the winter in a freeze-dried state and grow in the summer, experiencing a dynamic hydrologic regime as streamflow varies on a diel cycle and with weather conditions. During diel peaks in flow these streams transport particulate organic matter (POM) to the downstream closed-basin lakes. We investigated the spatial and temporal dynamics of POM transport derived from the scouring of microbial mats in Von Guerard Stream of the MDV. The results show clockwise hysteresis effects in POM concentration over diel flood pulses and suggests that POM transport in the MDVs is supply limited. Further studies are required to confirm this and to identify the potential contributing sources of POM. The hysteresis effect was modeled using an approach derived from models of sediment transport in streams. Spatial variations in POM transport indicate that patch-scale variations in bed shear stress and benthic biomass also influence transport which is integrated downstream over several 100's of meters. Large variations in the POM transport dynamics between different diel flood pulses were found to be related to the time since a resetting flood event and the regrowth of potentially mobile benthic biomass, providing further evidence of the importance of supply limitation and flow variability in controlling the organic matter flux of stream ecosystems.1 aCullis, James, D.S.1 aStanish, Lee, F.1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/diel-flow-pulses-drive-particulate-organic-matter-transport-microbial-mats-glacial-meltwater02372nas a2200193 4500008004100000245011200041210006900153260004000222490000900262520168100271653002401952653002301976653003801999653002402037653001002061100001602071700002402087856006702111 2014 eng d00aEcology and biogeography of freshwater diatoms in ponds of McMurdo Dry Valleys and parts of the Ross Island0 aEcology and biogeography of freshwater diatoms in ponds of McMur aBoulder, CObUniversity of Colorado0 vM.S.3 a

 

The McMurdo Dry Valleys (MDVs) and the exposed coastal areas of the nearby Ross Island in Antarctica represent some of the coldest, driest places in the world. However, during the austral summer warmer temperatures and constant sunlight allow microbial life to flourish nearly anywhere there is water. Diatoms are single-celled algae encapsulated in a silica shell and diatom communities constitute an important component of the microbial mats that grow in the streams, lakes and ponds in these regions. As part of the Long Term Ecological Research station in the McMurdo Dry Valleys diatom communities have been studied extensively in the streams over the last 20 years. Although the diatoms present in pond microbial mats at Cape Royds have been previously studied, modern-day knowledge of the characteristics of diatom communities in ponds and small lakes throughout the region is limited. This work sought to find the relationships between water chemistry and diatom community structure in ponds. Because water flow is not a factor in ponds and small lakes, influences of salinity, nutrients, pH and other factors can be more easily distinguished. This study looked at 24 separate bodies of water in the Taylor Valley, Labyrinth region in Wright Valley, Cape Royds and McMurdo Station area on Ross Island. The results suggest that geography, dispersal and historical environmental conditions play a significant role in structuring diatom communities, in addition to water chemistry. The results also expand the knowledge of habitat preferences for some of the species present in this region.

 

10abiological sciences10afreshwater diatoms10ahealth and environmental sciences10aMcMurdo Dry Valleys10aponds1 aSakaeva, A.1 aMcKnight, Diane, M. uhttps://search.proquest.com/docview/1655818365?accountid=1450303186nas a2200181 4500008004100000245010400041210006900145260003900214490001000253520255800263653002002821653001602841653002202857653001202879100002102891700002902912856006302941 2014 eng d00aEnvironmental impacts on RubisCO from green algal laboratory isolates to Antarctic lake communities0 aEnvironmental impacts on RubisCO from green algal laboratory iso aOxford, OHbMiami Universityc20140 vPh.D.3 a

Ribulose-1,5-bisphosphate carboxylase oxygenase (RubisCO) is found in a variety of autotrophic microorganisms ranging from green algae, cyanobacteria, and chemoautotrophic bacteria. As this enzyme has the potential to catalyze carboxylation (carbon fixation) or oxygenation (photorespiration) reactions, it is regulated in response to environmental variables at the levels of transcription, translation, and post-translation by the enzyme, RubisCO activase. A combination of laboratory experiments on green algal isolates and field experiments were utilized to gain insight on carbon fixation in permanently ice-covered Antarctic lakes. RubisCO was investigated as a potential target for cold adaptation of carbon fixation in the psychrophilic green alga, Chlamydomonas raudensis UWO241 (UWO241), isolated from Lake Bonney, Antarctica. RubisCO activity, stability, and whole cell carbon fixation were measured for the psychrophile and compared to a closely related mesophilic alga, C. raudensis SAG49.72 (SAG49.72). The effect of environmental factors including light and temperature on UWO241 and SAG49.72 RubisCO activation state, an indirect measurement of RubisCO activase activity, and abundance was investigated using a modified RubisCO carboxylase assay and immunoblotting, respectively. Lastly, maximum potential RubisCO carboxylase activity was determined using a modified activity assay in multiple ice covered Antarctic lakes including Lake Bonney. This data was complemented with lake depth profiles of enzyme abundance determined by quantitative real-time PCR and RubisCO-harboring organism diversity. While purified RubisCO of the psychrophilic green alga did not function optimally at low temperature, whole cell carbon fixation was greater under such conditions, suggesting that the overall process of carbon fixation is modified to function in UWO241. Increased RubisCO abundance at low temperature may contribute to this phenomenon. Low light levels may be important in regulation of RubisCO via RubisCO activase and should be further investigated. Based on community level RubsiCO activity and enzyme abundance, light and RubisCO harboring organisms including eukaryotic algae and cyanobacteria were positively correlated, but this was variable between lakes. Dark carbon fixation was potentially important in lakes west lobe Bonney and Fryxell and this community was negatively correlated with light. Results of targeted physiology and community level experiments led to development of a carbon fixation model for Lake Bonney.

10acarbon fixation10agreen algae10aice-covered lakes10aRubisCO1 aDolhi, Jenna, M.1 aMorgan-Kiss, Rachael, M. uhttp://rave.ohiolink.edu/etdc/view?acc_num=miami140705678300623nas a2200169 4500008004100000245009600041210006900137260001200206300001200218490000700230100002100237700001800258700002100276700002200297700001700319856011700336 2014 eng d00aRadiocarbon abundance and reservoir effects in lakes of the McMurdo Dry Valleys, Antarctica0 aRadiocarbon abundance and reservoir effects in lakes of the McMu c05/2014 a811-8260 v591 aDoran, Peter, T.1 aKenig, Fabien1 aKnoepfle, Lawson1 aMikucki, Jill, A.1 aLyons, Berry uhttps://mcm.lternet.edu/content/radiocarbon-abundance-and-reservoir-effects-lakes-mcmurdo-dry-valleys-antarctica03560nas a2200397 4500008004100000022001400041245005200055210004800107260001200155300001400167490000700181520243500188100001802623700002202641700001902663700002502682700001902707700002002726700002202746700002102768700002402789700002102813700002302834700002702857700002302884700002802907700001702935700001902952700002202971700002002993700002103013700002503034700002603059700002003085856005703105 2014 eng d a0012-961500aThe spatial structure of Antarctic biodiversity0 aspatial structure of Antarctic biodiversity c05/2014 a203 - 2440 v843 a

Patterns of environmental spatial structure lie at the heart of the most fundamental and familiar patterns of diversity on Earth. Antarctica contains some of the strongest environmental gradients on the planet and therefore provides an ideal study ground to test hypotheses on the relevance of environmental variability for biodiversity. To answer the pivotal question, “How does spatial variation in physical and biological environmental properties across the Antarctic drive biodiversity?” we have synthesized current knowledge on environmental variability across terrestrial, freshwater, and marine Antarctic biomes and related this to the observed biotic patterns. The most important physical driver of Antarctic terrestrial communities is the availability of liquid water, itself driven by solar irradiance intensity. Patterns of biota distribution are further strongly influenced by the historical development of any given location or region, and by geographical barriers. In freshwater ecosystems, free water is also crucial, with further important influences from salinity, nutrient availability, oxygenation, and characteristics of ice cover and extent. In the marine biome there does not appear to be one major driving force, with the exception of the oceanographic boundary of the Polar Front. At smaller spatial scales, ice cover, ice scour, and salinity gradients are clearly important determinants of diversity at habitat and community level. Stochastic and extreme events remain an important driving force in all environments, particularly in the context of local extinction and colonization or recolonization, as well as that of temporal environmental variability. Our synthesis demonstrates that the Antarctic continent and surrounding oceans provide an ideal study ground to develop new biogeographical models, including life history and physiological traits, and to address questions regarding biological responses to environmental variability and change.

 

1 aConvey, Peter1 aChown, Steven, L.1 aClarke, Andrew1 aBarnes, David, K. A.1 aBokhorst, Stef1 aCummings, Vonda1 aDucklow, Hugh, W.1 aFrati, Francesco1 aGreen, T., G. Allan1 aGordon, Shulamit1 aGriffiths, Huw, J.1 aHoward-Williams, Clive1 aHuiskes, Ad, H. L.1 aLaybourn-Parry, Johanna1 aLyons, Berry1 aMcMinn, Andrew1 aMorley, Simon, A.1 aPeck, Lloyd, S.1 aQuesada, Antonio1 aRobinson, Sharon, A.1 aSchiaparelli, Stefano1 aWall, Diana, H. uhttp://www.esajournals.org/doi/abs/10.1890/12-2216.102923nas a2200229 4500008004100000245012800041210006900169260001200238490000600250520215100256100001602407700002602423700002002449700002402469700002202493700001802515700002402533700001702557700002302574700001902597856007702616 2014 eng d00aSpring thaw ionic pulses boost nutrient availability and microbial growth in entombed Antarctic Dry Valley cryoconite holes0 aSpring thaw ionic pulses boost nutrient availability and microbi c12/20140 v53 a

The seasonal melting of ice entombed cryoconite holes on McMurdo Dry Valley glaciers provides oases for life in the harsh environmental conditions of the polar desert where surface air temperatures only occasionally exceed 0°C during the Austral summer. Here we follow temporal changes in cryoconite hole biogeochemistry on Canada Glacier from fully frozen conditions through the initial stages of spring thaw toward fully melted holes. The cryoconite holes had a mean isolation age from the glacial drainage system of 3.4 years, with an increasing mass of aqueous nutrients (dissolved organic carbon, total nitrogen, total phosphorus) with longer isolation age. During the initial melt there was a mean nine times enrichment in dissolved chloride relative to mean concentrations of the initial frozen holes indicative of an ionic pulse, with similar mean nine times enrichments in nitrite, ammonium, and dissolved organic matter. Nitrate was enriched twelve times and dissolved organic nitrogen six times, suggesting net nitrification, while lower enrichments for dissolved organic phosphorus and phosphate were consistent with net microbial phosphorus uptake. Rates of bacterial production were significantly elevated during the ionic pulse, likely due to the increased nutrient availability. There was no concomitant increase in photosynthesis rates, with a net depletion of dissolved inorganic carbon suggesting inorganic carbon limitation. Potential nitrogen fixation was detected in fully melted holes where it could be an important source of nitrogen to support microbial growth, but not during the ionic pulse where nitrogen availability was higher. This study demonstrates that ionic pulses significantly alter the timing and magnitude of microbial activity within entombed cryoconite holes, and adds credence to hypotheses that ionic enrichments during freeze-thaw can elevate rates of microbial growth and activity in other icy habitats, such as ice veins and subglacial regelation zones

1 aTelling, J.1 aAnesio, Alexandre, M.1 aTranter, Martyn1 aFountain, Andrew, G1 aNylen, Thomas, H.1 aHawkings, Jon1 aSingh, Virendra, B.1 aKaur, Preeti1 aMusilova, Michaela1 aWadham, J., L. uhttp://journal.frontiersin.org/article/10.3389/fmicb.2014.00694/abstract00634nas a2200181 4500008004100000245007700041210006900118260001200187490000600199100002100205700002400226700002300250700002000273700001900293700002400312700002000336856009600356 2013 eng d00aAccelerated thermokarst formation in the McMurdo Dry Valleys, Antarctica0 aAccelerated thermokarst formation in the McMurdo Dry Valleys Ant c07/20130 v31 aLevy, Joseph, S.1 aFountain, Andrew, G1 aDickson, James, L.1 aHead, James, W.1 aOkal, Marianne1 aMarchant, David, R.1 aWatters, Jaclyn uhttp://www.nature.com/srep/2013/130724/srep02269/full/srep02269.html?WT.ec_id=SREP-2013073002237nas a2200337 4500008004100000024003300041245010300074210006900177260001200246300001300258490000700271520126700278100001301545700001701558700001901575700001401594700002001608700001801628700001801646700001301664700001701677700001401694700002001708700001801728700001401746700002101760700001801781700002001799700001701819856006301836 2013 eng d ahdl: 10013 / epic.42531.d00100aAntarctic Thresholds - Ecosystem Resilience and Adaptation (AnT-ERA), a new SCAR-biology programme0 aAntarctic Thresholds Ecosystem Resilience and Adaptation AnTERA  c10/2013 a147-150.0 v823 a

Stresses on Antarctic ecosystems result from environmental change, including extreme events, and from (other) human impacts. Consequently, Antarctic habitats are changing, some at a rapid pace while others are relatively stable. A cascade of responses from molecular through organismic to the community level are expected. The differences in biological complexity and evolutionary histories between both polar regions and the rest of the planet suggest that stresses on polar ecosystem function may have fundamentally different outcomes from those at lower latitudes. Polar ecosystem processes are therefore key to informing wider ecological debate about the nature of stability and potential changes across the biosphere. The main goal of AnT-ERA is to facilitate the science required to examine changes in biological processes in Antarctic and sub-Antarctic marine-, freshwater and terrestrial ecosystems. Tolerance limits, as well as thresholds, resistance and resilience to environmental change will be determined. AnT-ERA is classified into three overlapping themes, which represent three levels of biological organisation: (1) molecular and physiological performance, (2) population processes and species traits, (3) ecosystem function and services.

1 aGutt, J.1 aAdams, Byron1 aBracegirdle, T1 aCowan, D.1 aCummings, Vonda1 adi Prisco, G.1 aGradinger, R.1 aIsla, E.1 aMcIntyre, T.1 aMurphy, E1 aPeck, Lloyd, S.1 aSchloss, I.R.1 aSmith, C.1 aSuckling, C., C.1 aTakahashi, A.1 aWall, Diana, H.1 aXavier, J.C. uhttp://epic.awi.de/34238/1/Polarforschung_82-2_147-150.pdf07866nas a2200205 4500008004100000022001400041245011200055210006900167260001200236300001400248490000700262520715200269100001907421700001907440700001807459700002707477700001807504700002107522856011707543 2013 eng d a1380-616500aCarbon Sequestration and Release from Antarctic Lakes: Lake Vida and West Lake Bonney (McMurdo Dry Valleys)0 aCarbon Sequestration and Release from Antarctic Lakes Lake Vida  c03/2013 a135 - 1450 v193 a

Perennial ice covers on many Antarctic lakes have resulted in high lake inorganic carbon contents. The objective of this paper was to evaluate and compare the brine and CO2 chemistries of Lake Vida (Victoria Valley) and West Lake Bonney (Taylor Valley), two lakes of the McMurdo Dry Valleys (East Antarctica), and their potential consequences during global warming. An existing geochemical model (FREZCHEM-15) was used to convert measured molarity into molality needed for the FREZCHEM model, and this model added a new algorithm that converts measured DIC into carbonate alkalinity needed for the FREZCHEM model. While quite extensive geochemical information exists for ice-covered Taylor Valley lakes, such as West Lake Bonney, only limited information exists for the recently sampled brine of >25 m ice-thick Lake Vida. Lake Vida brine had a model-calculated pCO2 = 0.60 bars at the field pH (6.20); West Lake Bonney had a model-calculated pCO2 = 5.23 bars at the field pH (5.46). Despite the high degree of atmospheric CO2supersaturation in West Lake Bonney, it remains significantly undersaturated with the gas hydrate, CO2·6H2O, unless these gas hydrates are deep in the sediment layer or are metastable having formed under colder temperatures or greater pressures. Because of lower temperatures, Lake Vida could start forming CO2·6H2O at lower pCO2 values than West Lake Bonney; but both lakes are significantly undersaturated with the gas hydrate, CO2·6H2O. For both lakes, simulation of global warming from current subzero temperatures (−13.4 °C in Lake Vida and −4.7 °C in West Lake Bonney) to 10 °C has shown that a major loss of solution-phase carbon as CO2 gases and carbonate minerals occurred when the temperatures rose above 0 °C and perennial ice covers would disappear. How important these Antarctic CO2 sources will be for future global warming remains to be seen. But a recent paper has shown that methane increased in atmospheric concentration due to deglaciation about 10,000 years ago. So, CO2 release from ice lakes might contribute to atmospheric gases in the future.

1 aMarion, G., M.1 aMurray, A., E.1 aWagner, Bernd1 aFritsen, Christian, H.1 aKenig, Fabien1 aDoran, Peter, T. uhttp://link.springer.com/10.1007/s10498-012-9184-1http://link.springer.com/content/pdf/10.1007/s10498-012-9184-100663nas a2200193 4500008004100000245008400041210006900125260001200194300001200206490000700218100001700225700001700242700001700259700001900276700002400295700001900319700002400338856010700362 2013 eng d00aThe carbon stable isotope biogeochemistry of streams, Taylor Valley, Antarctica0 acarbon stable isotope biogeochemistry of streams Taylor Valley A c05/2013 a26 - 360 v321 aLyons, Berry1 aLeslie, D.L.1 aHarmon, R.S.1 aNeumann, Klaus1 aWelch, Kathleen, A.1 aBisson, K., M.1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/carbon-stable-isotope-biogeochemistry-streams-taylor-valley-antarctica02340nas a2200241 4500008004100000245013800041210006900179260001200248300001100260490000600271520148800277100002301765700002401788700002301812700001901835700002001854700002201874700001901896700001701915700001801932700002701950856012101977 2013 eng d00aCharacterization of fulvic acid fractions of dissolved organic matter during ice-out in a hyper-eutrophic, coastal pond in Antarctica0 aCharacterization of fulvic acid fractions of dissolved organic m c12/2013 a0450150 v83 aDissolved humic material (HDOM) is ubiquitous to all natural waters and its source material influences its chemical structure, reactivity, and bioavailability. While terrestrially derived HDOM reference materials distributed by the International Humic Substances Society (IHSS) have been readily available to engineering and scientific communities, a microbially derived reference HDOM was not, despite the well-characterized differences in the chemistry and reactivity of HDOM derived from terrestrial versus microbial sources. To address this gap, we collected a microbial reference fulvic acid from Pony Lake (PLFA) for distribution through the IHSS. Pony Lake is a saline coastal pond on Ross Island, Antarctica, where the landscape is devoid of terrestrial plants. Sample collection occurred over a 17-day period in the summer season at Pony Lake. During this time, the dissolved organic carbon (DOC) concentrations increased nearly two-fold, and the fulvic acid fraction (collected using the XAD-8 method) accounted for 14.6% of the DOC. During the re-concentration and desalting procedures we isolated two other chemically distinct fulvic acid fractions: (1) PLFA-2, which was high in carbohydrates and (2) PLFA-CER, which was high in nitrogen. The chemical characteristics (elemental analysis, optical characterization with UV–vis and fluorescence spectroscopy, and 13C NMR spectroscopy) of the three fulvic acid fractions helped to explain their behavior during isolation.1 aCawley, Kaelin, M.1 aMcKnight, Diane, M.1 aMiller, Penney, L.1 aCory, Rose, M.1 aFimmen, Ryan, L1 aGuerard, Jennifer1 aDieser, Markus1 aJaros, Chris1 aChin, Yu-Ping1 aForeman, Christine, M. uhttps://mcm.lternet.edu/content/characterization-fulvic-acid-fractions-dissolved-organic-matter-during-ice-out-hyper00663nas a2200157 4500008004100000245019300041210006900234260001200303300001200315490000700327100002600334700002700360700002300387700002400410856007100434 2013 eng d00aCharacterization of IHSS Pony Lake fulvic acid dissolved organic matter by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and fluorescence spectroscopy0 aCharacterization of IHSS Pony Lake fulvic acid dissolved organic c12/2013 a19 - 280 v651 aD’Andrilli, Juliana1 aForeman, Christine, M.1 aMarshall, Alan, G.1 aMcKnight, Diane, M. uhttp://www.sciencedirect.com/science/article/pii/S014663801300216700625nas a2200145 4500008004100000245013000041210006900171260001100240490000600251100002300257700002000280700002100300700002400321856013400345 2013 eng d00aCORRIGENDUM: Don Juan Pond, Antarctica: Near-surface CaCl2-brine feeding Earth’s most saline lake and implications for Mars0 aCORRIGENDUM Don Juan Pond Antarctica Nearsurface CaCl2brine feed c3/20130 v31 aDickson, James, L.1 aHead, James, W.1 aLevy, Joseph, S.1 aMarchant, David, R. uhttps://mcm.lternet.edu/content/corrigendum-don-juan-pond-antarctica-near-surface-cacl2-brine-feeding-earth%E2%80%99s-most-saline00577nas a2200145 4500008004100000245011500041210006900156260001200225490000600237100002300243700002000266700002100286700002400307856010000331 2013 eng d00aDon Juan Pond, Antarctica: Near-surface CaCl2-brine feeding Earth's most saline lake and implications for Mars0 aDon Juan Pond Antarctica Nearsurface CaCl2brine feeding Earths m c01/20130 v31 aDickson, James, L.1 aHead, James, W.1 aLevy, Joseph, S.1 aMarchant, David, R. uhttp://www.nature.com/srep/2013/130130/srep01166/full/srep01166.html?WT.ec_id=SREP-704-2013020100588nas a2200169 4500008004100000245008700041210006900128260001200197300001100209490000600220100002100226700002300247700002400270700002400294700002000318856008000338 2013 eng d00aEnvironmental factors influencing diatom communities in Antarctic cryoconite holes0 aEnvironmental factors influencing diatom communities in Antarcti c12/2013 a0450060 v81 aStanish, Lee, F.1 aBagshaw, Elizabeth1 aMcKnight, Diane, M.1 aFountain, Andrew, G1 aTranter, Martyn uhttp://iopscience.iop.org/1748-9326/8/4/045006/pdf/1748-9326_8_4_045006.pdf00579nas a2200157 4500008004100000245012400041210006900165260001200234300001600246490000700262100001600269700002500285700002500310700002400335856006200359 2013 eng d00aThe influence of stream thermal regimes and preferential flow paths on hyporheic exchange in a glacial meltwater stream0 ainfluence of stream thermal regimes and preferential flow paths  c09/2013 a5552 - 55690 v491 aCozzetto, K1 aBencala, Kenneth, E.1 aGooseff, Michael, N.1 aMcKnight, Diane, M. uhttp://onlinelibrary.wiley.com/doi/10.1002/wrcr.20410/pdf02033nas a2200229 4500008004100000245011500041210006900156260001200225300001100237490000600248520129700254100002701551700001901578700002101597700002901618700002001647700002001667700002301687700001801710700002401728856005101752 2013 eng d00aMicrobial growth under humic-free conditions in a supraglacial stream system on the Cotton Glacier, Antarctica0 aMicrobial growth under humicfree conditions in a supraglacial st c09/2013 a0350220 v83 aDuring the austral summers of 2004 and 2009, we sampled a supraglacial stream on the Cotton Glacier, Antarctica. The stream dissolved organic matter (DOM) was low (44–48 μM C) and lacked detectable humic fluorescence signatures. Analysis of the excitation emissions matrices (EEMs) indicated that amino-acid fluorophores dominated, consistent with DOM of microbial origin, with little humic-like fluorescence. In most aquatic ecosystems, humic DOM attenuates harmful UV radiation and its absence may represent an additional stressor influencing the microbial community. Nonetheless, the stream contained an active microbial assemblage with bacterial cell abundances from 2.94 × 104 to 4.97 × 105 cells ml−1, and bacterial production ranging from 58.8 to 293.2 ng C l−1 d−1. Chlorophyll-a concentrations ranged from 0.3 to 0.53 μg l−1 indicating that algal phototrophs were the probable source of the DOM. Microbial isolates produced a rainbow of pigment colors, suggesting adaptation to stress, and were similar to those from other cryogenic systems (Proteobacteria and Bacteroidetes lineages). Supraglacial streams provide an example of contemporary microbial processes on the glacier surface and a natural laboratory for studying microbial adaptation to the absence of humics.1 aForeman, Christine, M.1 aCory, Rose, M.1 aMorris, Cindy, E1 aSanClements, Michael, D.1 aSmith, Heidi, J1 aLisle, John, T.1 aMiller, Penney, L.1 aChin, Yu-Ping1 aMcKnight, Diane, M. uhttp://iopscience.iop.org/1748-9326/8/3/03502200803nas a2200217 4500008004100000245011600041210006900157260001200226300001400238490000700252100001900259700002700278700001700305700002000322700002400342700002800366700002300394700001800417700002400435856012600459 2013 eng d00aPhysicochemical and biological dynamics in a coastal Antarctic lake as it transitions from frozen to open water0 aPhysicochemical and biological dynamics in a coastal Antarctic l c12/2013 a663–6750 v251 aDieser, Markus1 aForeman, Christine, M.1 aJaros, Chris1 aLisle, John, T.1 aGreenwood, Mark, C.1 aLaybourn-Parry, Johanna1 aMiller, Penney, L.1 aChin, Yu-Ping1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/physicochemical-and-biological-dynamics-coastal-antarctic-lake-it-transitions-frozen-open00753nas a2200193 4500008004100000245014000041210006900181260001200250300000800262490000700270100002100277700002300298700001700321700002500338700002400363700002400387700002200411856012600433 2012 eng d00aAntarctic Tardigrada: a first step in understanding molecular operational taxonomic units (MOTUs) and biogeography of cryptic meiofauna0 aAntarctic Tardigrada a first step in understanding molecular ope c12/2012 a5260 v261 aCzechowski, Paul1 aSands, Chester, J.1 aAdams, Byron1 aD'Haese, Cyrille, A.1 aGibson, John, A. E.1 aMcInnes, Sandra, J.1 aStevens, Mark, I. uhttps://mcm.lternet.edu/content/antarctic-tardigrada-first-step-understanding-molecular-operational-taxonomic-units-motus02734nas a2200229 4500008004100000245012900041210006900170260001600239300001100255490000600266520197000272100002402242700002202266700001902288700001602307700002202323700002002345700001702365700002102382700002402403856007702427 2012 eng d00aAt Limits of Life: Multidisciplinary Insights Reveal Environmental Constraints on Biotic Diversity in Continental Antarctica0 aAt Limits of Life Multidisciplinary Insights Reveal Environmenta cJul-09-2013 ae445780 v73 a

Multitrophic communities that maintain the functionality of the extreme Antarctic terrestrial ecosystems, while the simplest of any natural community, are still challenging our knowledge about the limits to life on earth. In this study, we describe and interpret the linkage between the diversity of different trophic level communities to the geological morphology and soil geochemistry in the remote Transantarctic Mountains (Darwin Mountains, 80°S). We examined the distribution and diversity of biota (bacteria, cyanobacteria, lichens, algae, invertebrates) with respect to elevation, age of glacial drift sheets, and soil physicochemistry. Results showed an abiotic spatial gradient with respect to the diversity of the organisms across different trophic levels. More complex communities, in terms of trophic level diversity, were related to the weakly developed younger drifts (Hatherton and Britannia) with higher soil C/N ratio and lower total soluble salts content (thus lower conductivity). Our results indicate that an increase of ion concentration from younger to older drift regions drives a succession of complex to more simple communities, in terms of number of trophic levels and diversity within each group of organisms analysed. This study revealed that integrating diversity across multi-trophic levels of biotic communities with abiotic spatial heterogeneity and geological history is fundamental to understand environmental constraints influencing biological distribution in Antarctic soil ecosystems.

 
1 aMagalhaes, Catarina1 aStevens, Mark, I.1 aCary, Craig, S1 aBall, Becky1 aStorey, Bryan, C.1 aWall, Diana, H.1 aTürk, Roman1 aRuprecht, Ulrike1 ade Bello, Francesco uhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.004457800656nas a2200181 4500008004100000245009100041210006900132260001200201100002100213700002200234700002300256700002100279700002000300700002400320700002500344700002400369856008100393 2012 eng d00aBacteria and diatom co-occurrence patterns in microbial mats from polar desert streams0 aBacteria and diatom cooccurrence patterns in microbial mats from c10/20121 aStanish, Lee, F.1 aO'Neill, Sean, P.1 aGonzález, Antonio1 aLegg, Teresa, M.1 aKnelman, Joseph1 aMcKnight, Diane, M.1 aSpaulding, Sarah, A.1 aNemergut, Diana, R. uhttp://onlinelibrary.wiley.com/doi/10.1111/j.1462-2920.2012.02872.x/abstract04191nas a2200445 4500008004100000022001400041245005900055210005900114260001600173300001400189490000800203520302300211100002203234700001603256700001903272700001503291700001803306700002003324700001503344700001903359700001603378700001303394700001503407700001803422700001303440700002203453700001503475700001503490700002303505700001303528700001903541700001703560700001503577700001703592700001803609700002003627700001603647700002003663856006203683 2012 eng d a0036-807500aChallenges to the Future Conservation of the Antarctic0 aChallenges to the Future Conservation of the Antarctic cJan-07-2013 a158 - 1590 v3373 a

The Antarctic Treaty System, acknowledged as a successful model of cooperative regulation of one of the globe's largest commons (1), is under substantial pressure. Concerns have been raised about increased stress on Antarctic systems from global environmental change and growing interest in the region's resources (23). Although policy-makers may recognize these challenges, failure to respond in a timely way can have substantial negative consequences. We provide a horizon scan, a systematic means for identifying emerging trends and assisting decision-makers in identifying policies that address future challenges (23). Previous analyses of conservation threats in the Antarctic have been restricted to matters for which available evidence is compelling (4). We reconsider these concerns because they might escalate quickly, judging from recent rapid environmental change in parts of Antarctica and increasing human interest in the region (see the map). We then focus on a more distant time horizon.

1 aChown, Steven, L.1 aLee, J., E.1 aHughes, K., A.1 aBarnes, J.1 aBarrett, P.J.1 aBergstrom, D.M.1 aConvey, P.1 aCowan, Don, A.1 aCrosbie, K.1 aDyer, G.1 aFrenot, Y.1 aGrant, S., M.1 aHerr, D.1 aKennicutt, M., C.1 aLamers, M.1 aMurray, A.1 aPossingham, H., P.1 aReid, K.1 aRiddle, M., J.1 aRyan, P., G.1 aSanson, L.1 aShaw, J., D.1 aSparrow, M.D.1 aSummerhayes, C.1 aTerauds, A.1 aWall, Diana, H. uhttp://www.sciencemag.org/cgi/doi/10.1126/science.122282100624nas a2200169 4500008004100000245011500041210006900156260001200225300001600237490000700253100002500260700002100285700002100306700002200327700002500349856008000374 2012 eng d00aChemical analysis of ice vein microenvironments: II. Analysis of glacial samples from Greenland and Antarctica0 aChemical analysis of ice vein microenvironments II Analysis of g c12/2012 a1109 - 11180 v581 aBarletta, Robert, E.1 aPriscu, John, C.1 aMader, Heidy, M.1 aJones, Warren, L.1 aRoe, Christopher, H. uhttp://www.montana.edu/priscu/DOCS/Publications/BarlettaEtAl2012IceVein.pdf00532nas a2200145 4500008004100000245009700041210006900138260001200207300001400219490000700233100002700240700002100267700002100288856007700309 2012 eng d00aCyanobacterial diversity across landscape units in a polar desert: Taylor Valley, Antarctica0 aCyanobacterial diversity across landscape units in a polar deser c11/2012 a268 - 2780 v821 aMichaud, Alexander, B.1 aŠabacká, Marie1 aPriscu, John, C. uhttp://onlinelibrary.wiley.com/doi/10.1111/j.1574-6941.2012.01297.x/full00561nas a2200157 4500008004100000245012400041210006900165260001200234300001400246490000700260100001800267700002000285700002100305700002900326856004800355 2012 eng d00aDiversity and Expression of RubisCO Genes in a Perennially Ice-Covered Antarctic Lake during the Polar Night Transition0 aDiversity and Expression of RubisCO Genes in a Perennially IceCo c04/2012 a4358-43660 v781 aKong, Weidong1 aReam, David, C.1 aPriscu, John, C.1 aMorgan-Kiss, Rachael, M. uhttp://aem.asm.org/content/78/12/4358.short00640nas a2200193 4500008004100000245010100041210006900142260001200211300001000223490000600233100002200239700002000261700001700281700002300298700001600321700002500337700002400362856006000386 2012 eng d00aThe ecology of pulse events: insights from an extreme climatic event in a polar desert ecosystem0 aecology of pulse events insights from an extreme climatic event  c02/2012 aart170 v31 aNielsen, Uffe, N.1 aWall, Diana, H.1 aAdams, Byron1 aVirginia, Ross, A.1 aBall, Becky1 aGooseff, Michael, N.1 aMcKnight, Diane, M. uhttp://www.esajournals.org/doi/abs/10.1890/ES11-00325.100583nas a2200169 4500008004100000245008400041210006900125260001200194300001400206490000700220100001800227700002100245700002000266700002100286700002900307856007700336 2012 eng d00aEvidence of form II RubisCO ( cbbM) in a perennially ice-covered Antarctic lake0 aEvidence of form II RubisCO cbbM in a perennially icecovered Ant c11/2012 a491 - 5000 v821 aKong, Weidong1 aDolhi, Jenna, M.1 aChiuchiolo, Amy1 aPriscu, John, C.1 aMorgan-Kiss, Rachael, M. uhttp://onlinelibrary.wiley.com/doi/10.1111/j.1574-6941.2012.01431.x/full00743nas a2200205 4500008004100000245013500041210006900176260001200245300001600257490000700273100002100280700002200301700002500323700002400348700002200372700002000394700002400414700002400438856007500462 2012 eng d00aExtreme streams: flow intermittency as a control on diatom communities in meltwater streams in the McMurdo Dry Valleys, Antarctica0 aExtreme streams flow intermittency as a control on diatom commun c08/2012 a1405 - 14190 v691 aStanish, Lee, F.1 aKohler, Tyler, J.1 aEsposito, Rhea, M.M.1 aSimmons, Breana, L.1 aNielsen, Uffe, N.1 aWall, Diana, H.1 aNemergut, Diana, R.1 aMcKnight, Diane, M. uhttp://www.nrcresearchpress.com/doi/abs/10.1139/f2012-022#.UH2NlWcnq9g00619nas a2200193 4500008004100000245006400041210005800105260001100163300001100174490000700185100001700192700002400209700002900233700001700262700002400279700001600303700002100319856008500340 2012 eng d00aThe geochemistry of upland ponds, Taylor Valley, Antarctica0 ageochemistry of upland ponds Taylor Valley Antarctica c2/2012 a3 - 140 v241 aLyons, Berry1 aWelch, Kathleen, A.1 aGardner, Christopher, B.1 aJaros, Chris1 aMoorhead, Daryl, L.1 aKnoepfle, J1 aDoran, Peter, T. uhttp://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=848335101017nas a2200337 4500008004100000022001400041245007500055210006900130260001200199300001800211490000800229100001900237700001800256700002700274700002700301700002300328700002000351700001900371700002400390700002500414700001700439700001800456700002100474700002700495700002400522700001800546700001900564700001900583700002100602856005600623 2012 eng d a0027-842400aMicrobial life at -13  C in the brine of an ice-sealed Antarctic lake0 aMicrobial life at 13 C in the brine of an icesealed Antarctic la c12/2012 a20626 - 206310 v1091 aMurray, A., E.1 aKenig, Fabien1 aFritsen, Christian, H.1 aMcKay, Christopher, P.1 aCawley, Kaelin, M.1 aEdwards, R., L.1 aKuhn, Emanuele1 aMcKnight, Diane, M.1 aOstrom, Nathaniel, E1 aPeng, Vivian1 aPonce, Adrian1 aPriscu, John, C.1 aSamarkin, Vladimir, A.1 aTownsend, Ashley, T1 aWagh, Protima1 aYoung, Seth, A1 aYung, Pung, To1 aDoran, Peter, T. uhttp://www.pnas.org/cgi/doi/10.1073/pnas.120860710901724nam a2200385 4500008004100000020002200041245011900063210006900182260005100251300001400302490000900316520052100325100002500846700002200871700001900893700002500912700002700937700002000964700002300984700002101007700002101028700001801049700001901067700001901086700001901105700002201124700001401146700002001160700002101180700002101201700002001222700001901242700001901261856005801280 2012 eng d a978-3-642-33179-400aPoisson Reconstruction of Extreme Submersed Environments: The ENDURANCE Exploration of an Under-Ice Antarctic Lake0 aPoisson Reconstruction of Extreme Submersed Environments The END aBerlin, HeidelbergbSpringer Berlin Heidelberg a394 - 4030 v74313 a

We evaluate the use of Poisson reconstruction to generate a 3D bathymetric model of West Lake Bonney, Antarctica. The source sonar dataset has been collected by the ENDURANCE autonomous ve- hicle in the course of two Antarctic summer missions. The reconstruction workflow involved processing 200 million datapoints to generate a high resolution model of the lake bottom, Narrows region and underwater glacier face. A novel and flexible toolset has been developed to automate the processing of the Bonney data.

1 aFebretti, Alessandro1 aRichmond, Kristof1 aGulati, Shilpa1 aFlesher, Christopher1 aHogan, Bartholomew, P.1 aJohnson, Andrew1 aStone, William, C.1 aPriscu, John, C.1 aDoran, Peter, T.1 aBebis, George1 aBoyle, Richard1 aParvin, Bahram1 aKoracin, Darko1 aFowlkes, Charless1 aWang, Sen1 aChoi, Min-Hyung1 aMantler, Stephan1 aSchulze, Jürgen1 aAcevedo, Daniel1 aMueller, Klaus1 aPapka, Michael uhttp://www.springerlink.com/content/hg97w43588229087/00682nas a2200169 4500008004100000245014300041210006900184260001200253300001400265490000700279100001800286700001800304700001900322700002400341700002400365856012300389 2011 eng d00a15N and 13C{14N} NMR investigation of the major nitrogen-containing segment in an aquatic fulvic acid: Evidence for a hydantoin derivative0 a15N and 13C 14N NMR investigation of the major nitrogencontainin c12/2011 a775 - 7800 v491 aFang, Xiaowen1 aMao, Jingdong1 aCory, Rose, M.1 aMcKnight, Diane, M.1 aSchmidt-Rohr, Klaus uhttps://mcm.lternet.edu/content/15n-and-13c14n-nmr-investigation-major-nitrogen-containing-segment-aquatic-fulvic-acid00722nas a2200193 4500008004100000245012800041210006900169260001200238300001400250490000700264100002300271700001900294700001500313700002000328700001600348700002400364700001600388856012400404 2011 eng d00aDetermination of Dissolved Oxygen in the Cryosphere: A Comprehensive Laboratory and Field Evaluation of Fiber Optic Sensors0 aDetermination of Dissolved Oxygen in the Cryosphere A Comprehens c01/2011 a700 - 7050 v451 aBagshaw, Elizabeth1 aWadham, J., L.1 aMowlem, M.1 aTranter, Martyn1 aEveness, J.1 aFountain, Andrew, G1 aTelling, J. uhttps://mcm.lternet.edu/content/determination-dissolved-oxygen-cryosphere-comprehensive-laboratory-and-field-evaluation03418nas a2200217 4500008004100000245009200041210006900133260004000202490001000242520270900252653001502961653002402976653002203000653001203022653001903034653002403053653001203077100002103089700002403110856006603134 2011 eng d00aEcological controls on stream diatom communities in the McMurdo Dry Valleys, Antarctica0 aEcological controls on stream diatom communities in the McMurdo  aBoulder, CObUniversity of Colorado0 vPh.D.3 a

 

Diatoms are vital primary producers in aquatic ecosystems and useful indicators of environmental change. In climatically sensitive polar areas, diatoms have been used as beacons of climate change, allowing us to monitor physical, chemical, and biological changes. This research aims to improve our understanding of diatom ecology in the pristine and dynamic McMurdo Dry Valleys of Antarctica, where diatoms reside in stream microbial mats. These results aid in understanding how changes in hydrologic regime will affect stream microbial communities, biodiversity, and ecosystem function in a changing environment.

Relationships between hydrologic regime and diatom community composition were investigated using long-term data. Diatom communities were structured by stream physical features, and streams with more similar hydrologic characteristics had more similar communities. Variation in diatom community composition was best explained by hydrologic regime. Small diatoms increased in relative abundances with increasing streamflow, suggesting a role of diatom size in structuring communities. Overall, diatom communities were resistant to flood and drought-like conditions, suggesting an adaptation to frequent disturbances.

The importance of hydrologic regime on drift activity was investigated during three 24- hour experiments. Diel variations in drift could be attributed to diurnal flow peaks. Biomass and diatom cell densities followed a clockwise pattern with stream discharge and support the dominant role of hydraulic processes. The quality of source material differed between seasons and throughout the day. Drifting diatom communities were dominated by Fistulifera pelliculosa, which is rarely found in stream mats and suggests a different source. Modeling results suggest that the less firmly anchored marginal mats contribute more to the drift than channel mats in low- flow seasons, while the channel mats become more important during high-flow seasons.

The relationships between diatom and bacterial assemblages in microbial mats were assessed based on phylogenetic and functional relatedness in five Dry Valley streams. Significant relationships between diatom and bacterial communities were found, and co-occurrence analysis identified numerous correlations between individual diatom and bacterial taxa. A consistency in metabolic lifestyles of correlated taxa suggests that the relationships are ecologically relevant. Diatom and bacterial diversity showed opposite patterns, which indicate differences in environmental drivers of diversity for bacteria and eukaryotes.

 

10aAntarctica10abiological sciences10acommunity ecology10adiatoms10aearth sciences10aMcMurdo Dry Valleys10astreams1 aStanish, Lee, F.1 aMcKnight, Diane, M. uhttps://search.proquest.com/docview/915694114?accountid=1450300636nas a2200169 4500008004100000245009700041210006900138260001200207300001600219490000700235100002300242700002000265700001900285700002400304700001500328856012300343 2011 eng d00aHigh-resolution monitoring reveals dissolved oxygen dynamics in an Antarctic cryoconite hole0 aHighresolution monitoring reveals dissolved oxygen dynamics in a c08/2011 a2868 - 28770 v251 aBagshaw, Elizabeth1 aTranter, Martyn1 aWadham, J., L.1 aFountain, Andrew, G1 aMowlem, M. uhttps://mcm.lternet.edu/content/high-resolution-monitoring-reveals-dissolved-oxygen-dynamics-antarctic-cryoconite-hole00641nas a2200181 4500008004100000245011500041210006900156260001100225300001400236490000700250100001800257700002100275700002100296700001800317700001900335700002000354856008500374 2011 eng d00aThe Holocene environmental history of Lake Hoare, Taylor Valley, Antarctica, reconstructed from sediment cores0 aHolocene environmental history of Lake Hoare Taylor Valley Antar c6/2011 a307 - 3190 v231 aWagner, Bernd1 aOrtlepp, Sabrina1 aDoran, Peter, T.1 aKenig, Fabien1 aMelles, Martin1 aBurkemper, Andy uhttp://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=827537400494nas a2200145 4500008004100000245008600041210006900127260001200196300001400208490000700222100002100229700002400250700002400274856005000298 2011 eng d00aHydrologic processes influence diatom community composition in Dry Valley streams0 aHydrologic processes influence diatom community composition in D c12/2011 a1057-10730 v301 aStanish, Lee, F.1 aNemergut, Diana, R.1 aMcKnight, Diane, M. uhttp://www.jnabs.org/doi/abs/10.1899/11-008.100581nas a2200169 4500008004100000245008600041210006900127260001200196300001400208490000600222100002500228700002400253700002100277700002400298700001700322856007200339 2011 eng d00aHydrological Connectivity of the Landscape of the McMurdo Dry Valleys, Antarctica0 aHydrological Connectivity of the Landscape of the McMurdo Dry Va c09/2011 a666 - 6810 v51 aGooseff, Michael, N.1 aMcKnight, Diane, M.1 aDoran, Peter, T.1 aFountain, Andrew, G1 aLyons, Berry uhttp://onlinelibrary.wiley.com/doi/10.1111/j.1749-8198.2011.00445.x00623nas a2200181 4500008004100000245010000041210006900141260001100210300001600221490000600237100002100243700002100264700001800285700002000303700002100323700002900344856006800373 2011 eng d00aProtist diversity in a permanently ice-covered Antarctic Lake during the polar night transition0 aProtist diversity in a permanently icecovered Antarctic Lake dur c9/2011 a1559 - 15640 v51 aBielewicz, Scott1 aBell, Elanor, R.1 aKong, Weidong1 aFriedberg, Iddo1 aPriscu, John, C.1 aMorgan-Kiss, Rachael, M. uhttp://www.nature.com/ismej/journal/v5/n9/abs/ismej201123a.html02193nas a2200145 4500008004100000245014200041210006900183260004000252300000800292490001000300520162300310100002401933700002401957856006601981 2011 eng d00aRemoval of benthic algae in swift-flowing streams: The significance of spatial and temporal variation in shear stress and bed disturbance0 aRemoval of benthic algae in swiftflowing streams The significanc aBoulder, CObUniversity of Colorado a2630 vPh.D.3 a

 

In this thesis I investigate the role of spatial and temporal dynamics of flow, bed shear stress, and bed disturbance on the removal of benthic algal and the consequences for our understanding of the dynamics of stream ecosystems and the transport of organic matter in fluvial systems. I use data obtained from two specific case studies where benthic algal mats are key components in the overall system dynamics. The first case study investigates spatial and temporal variations in particular organic matter transport derived from the removal of benthic algae in a stream in the McMurdo Dry Valleys of Antarctica. The second case study uses a combination of field observations and analysis, one and two dimensional hydraulic modeling, and laboratory flume studies to investigate the controls on the removal of the stalk forming diatom Didymosphenia geminata. The results show that benthic algae growing in swift-flowing streams are well adapted to this environment. Hence the removal of organic material due to elevated shear stress alone is limited and effective control of nuisance blooms of benthic algae such as D. geminata requires flood events sufficiently high enough to results in wide spread disturbance of the stream substrate. These flow requirements are similar to the requirements for channel maintenance flows and could therefore be considered for inclusion in the operating rules for dams in order to maintain the diversity of stream ecosystems and functioning water supply infrastructure.

 

1 aCullis, James, D.S.1 aMcKnight, Diane, M. uhttps://search.proquest.com/docview/916604693?accountid=1450300608nas a2200133 4500008004100000245016200041210006900203260000900272490000700281100001300288700002400301700002100325856012800346 2011 eng d00aSimulating unsteady flow, anabranching, and hyporheic dynamics in a glacial meltwater stream using a coupled surface water routing and groundwater flow model0 aSimulating unsteady flow anabranching and hyporheic dynamics in  c20110 v471 aKoch, J.1 aMcKnight, Diane, M.1 aNeupauer, R., M. uhttps://mcm.lternet.edu/content/simulating-unsteady-flow-anabranching-and-hyporheic-dynamics-glacial-meltwater-stream-using00685nam a2200193 4500008004100000020002200041245010600063210006900169260003600238300001400274490000800288100001900296700002500315700002400340700002300364700002600387700002000413856005800433 2011 eng d a978-94-007-1363-500aSpectral Methods to Advance Understanding of Dissolved Organic Carbon Dynamics in Forested Catchments0 aSpectral Methods to Advance Understanding of Dissolved Organic C aDordrechtbSpringer Netherlands a117 - 1350 v2161 aCory, Rose, M.1 aBoyer, Elizabeth, W.1 aMcKnight, Diane, M.1 aLevia, Delphis, F.1 aCarlyle-Moses, Darryl1 aTanaka, Tadashi uhttp://www.springerlink.com/content/lv9365ml54192m29/00776nas a2200229 4500008004100000245011100041210006900152260001200221300001400233490000700247100002700254700001900281700002000300700001900320700002800339700002000367700001700387700002300404700001800427700002400445856007700469 2011 eng d00aWhen a habitat freezes solid: microorganisms over-winter within the ice column of a coastal Antarctic lake0 aWhen a habitat freezes solid microorganisms overwinter within th c06/2011 a401 - 4120 v761 aForeman, Christine, M.1 aDieser, Markus1 aGreenwood, Mark1 aCory, Rose, M.1 aLaybourn-Parry, Johanna1 aLisle, John, T.1 aJaros, Chris1 aMiller, Penney, L.1 aChin, Yu-Ping1 aMcKnight, Diane, M. uhttp://onlinelibrary.wiley.com/doi/10.1111/j.1574-6941.2011.01061.x/full00655nas a2200193 4500008004100000245008400041210006900125260001100194300001400205490000600219100002700225700002500252700002500277700002500302700002100327700002700348700002300375856006300398 2010 eng d00aAbiotic nitrous oxide emission from the hypersaline Don Juan Pond in Antarctica0 aAbiotic nitrous oxide emission from the hypersaline Don Juan Pon c5/2010 a341 - 3440 v31 aSamarkin, Vladimir, A.1 aMadigan, Michael, T.1 aBowles, Marshall, W.1 aCasciotti, Karen, L.1 aPriscu, John, C.1 aMcKay, Christopher, P.1 aJoye, Samantha, B. uhttp://www.nature.com/ngeo/journal/v3/n5/full/ngeo847.html00722nas a2200193 4500008004100000020001800041245008800059210006900147260004200216300001400258100002500272700002400297700001700321700001700338700002100355700001700376700002400393856011100417 2010 eng d a978052188919300aAntarctic McMurdo Dry Valley stream ecosystems as analog to fluvial systems on Mars0 aAntarctic McMurdo Dry Valley stream ecosystems as analog to fluv aCambridgebCambridge University Press a139 - 1591 aGooseff, Michael, N.1 aMcKnight, Diane, M.1 aCarr, M., H.1 aBaeseman, J.1 aDoran, Peter, T.1 aLyons, Berry1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/antarctic-mcmurdo-dry-valley-stream-ecosystems-analog-fluvial-systems-mars00861nas a2200253 4500008004100000245013000041210006900171260001200240300001400252490000800266100001300274700002600287700001500313700001400328700002200342700001500364700001500379700001500394700001400409700001600423700002100439700002100460856012600481 2010 eng d00aDesign and deployment of a four-degrees-of-freedom hovering autonomous underwater vehicle for sub-ice exploration and mapping0 aDesign and deployment of a fourdegreesoffreedom hovering autonom c11/2010 a341 - 3610 v2241 aStone, W1 aHogan, Bartholomew, P1 aFlesher, C1 aGulati, S1 aRichmond, Kristof1 aMurarka, A1 aKuhlman, G1 aIdharan, M1 aSiegel, V1 aPrice, R, M1 aDoran, Peter, T.1 aPriscu, John, C. uhttps://mcm.lternet.edu/content/design-and-deployment-four-degrees-freedom-hovering-autonomous-underwater-vehicle-sub-ice00711nas a2200181 4500008004100000020001800041245009200059210006900151250000600220260003100226100002500257700002400282700001500306700002400321700001900345700002100364856014400385 2010 eng d a978052150996100aDiatoms as indicators of environmental change in Antarctic and subantarctic freshwaters0 aDiatoms as indicators of environmental change in Antarctic and s a2 bCambridge University Press1 aSpaulding, Sarah, A.1 aVan de Vijver, Bart1 aHodgson, D1 aMcKnight, Diane, M.1 aVerleyen, Elie1 aStanish, Lee, F. uhttp://www.cambridge.org/us/academic/subjects/life-sciences/plant-science/diatoms-applications-environmental-and-earth-sciences-2nd-edition00568nas a2200169 4500008004100000245009500041210006900136260001200205300001000217490000600227100001900233700002300252700002400275700002200299700002300321856005400344 2010 eng d00aEffect of instrument-specific response on the analysis of fulvic acid fluorescence spectra0 aEffect of instrumentspecific response on the analysis of fulvic  c07/2010 a67-780 v81 aCory, Rose, M.1 aMiller, Mathew, P.1 aMcKnight, Diane, M.1 aGuerard, Jennifer1 aMiller, Penney, L. uhttp://www.aslo.org/lomethods/free/2010/0067.html02203nas a2200217 4500008004100000245008900041210006900130260001200199300001100211490000800222520155200230653001501782653002601797653002301823653002101846653001801867100001301885700002401898700001701922856004601939 2010 eng d00aEffect of unsteady flow on nitrate loss in an oligotrophic, glacial meltwater stream0 aEffect of unsteady flow on nitrate loss in an oligotrophic glaci c03/2010 aG010010 v1153 a

The McMurdo Dry Valleys of Antarctica are among the coldest, driest ecosystems on Earth. During the austral summer, glacial meltwater supports cyanobacterial mat communities in some streams, but they are not ubiquitous. We conducted a nitrate (NO3-) enrichment tracer injection in Huey Creek to quantify NO3- loss in a Dry Valley stream where algal mats would not obscure hyporheic microbial processes. Unsteady streamflow led to diel variability in the tracer concentration and in surface/subsurface water and solute exchange. Subsequently, concentrations of NO3-, nitrite (NO2-), ammonium (NH4+), and dissolved organic carbon (DOC) varied significantly during the injection, with a net loss of NO3-, NO2-, and DOC, and production of nitrous oxide. These mass changes within a reach were often coincident with high streamflows. Reactivity also coincided with the highest DOC concentrations, suggesting that DOC is the primary limitation to heterotrophic microbial activity in the stream. Together, streamflow and DOC availability create the hot spots and hot moments that dominate NO3- reactivity and removal in this polar desert ecosystem. The combination of spatially and temporally variable hyporheic dynamics and solute availability underscore the limitations of common nutrient uptake metrics and transient storage models when unsteady flow conditions exist.

10aAntarctica10ahot spots/hot moments10ahyporheic exchange10anitrogen cycling10aunsteady flow1 aKoch, J.1 aMcKnight, Diane, M.1 aBaeseman, J. uhttp://doi.wiley.com/10.1029/2009JG00103003183nas a2200229 4500008004100000245011200041210006900153260004600222300000800268490001000276520245700286653001102743653001502754653001902769653001302788653001302801653001502814653003702829100001302866700002402879856005002903 2010 eng d00aHydrologic and biogeochemical implications of flooding in two catchments underlain by continuous permafrost0 aHydrologic and biogeochemical implications of flooding in two ca aBoulder, CObUniversity of Coloradoc2010 a2060 vPh.D.3 a

Flooding is a critical driver of ecosystem productivity. By rapidly increasing stream stage and velocity, floods mix water and solutes from the stream, hyporheic zone, and floodplains/riparian areas. Such mixing may spur biogeochemical activity. In catchments underlain by permafrost, flooding is more common due to both the potential for rapid ice melting and minimal storage potential in frozen soils. High latitude environments are often underlain by permafrost and are also areas of biogeochemical interest, due to large stores of carbon (C) and nitrogen (N), and the potential for rapid cycling. The increased complexity in groundwater/surface water hydrology during floods requires rigorous hydrologic analysis before biogeochemical trends can be correctly interpreted. This research aims to accurately quantify the hydrology and biogeochemical cycling of C and N in two high-latitude catchments utilizing stream tracer additions, synoptic sampling, and surface water (sw), groundwater (gw), and coupled sw/gw flow models.

Two catchments, in Alaska and Antarctica represent very different ecosystems, both characterized by continuous permafrost and shallow aquifers. In Antarctica, coupled surface water/groundwater flow modeling and tracer additions identify sources of DOC (dissolved organic carbon) and locations of denitrification. Mass balance calculations identify heightened water/sediment interactions at high flows, and increased C and N uptake when solutes return to the stream during low flows. In Alaska, discharge correlates to DOC and nitrate concentrations, indicating leaching and flushing of organic material from the hillslope during high discharge, with a greater potential for microbial processing of this organic material during low flows. Multiple tracer additions demonstrate a seasonal trend, with the greatest C and N uptake early in the summer, potentially related to shallower flowpaths.

Differences between discharge, flooding, and C and N cycling in these two catchments indicate the importance of stream size and morphology. Using tracer dilution and major ion and uranium isotope chemistry, we identify preferential flow near and beneath the stream, indicating erosion of the stream bed via soil piping and thermokarsting. We propose that channel evolution will lead to decreased stream/catchment interactions and subsequently decreased C and N uptake potential in these high-latitude catchments.

10acarbon10acatchments10aearth sciences10aflooding10anitrogen10apermafrost10asurface-groundwater interactions1 aKoch, J.1 aMcKnight, Diane, M. uhttps://search.proquest.com/docview/83900886500763nam a2200241 4500008004100000020001800041245005800059210005800117260004300175300000800218490000600226653002200232100002100254700001700275700002400292700002400316700002500340700003300365700002200398700002000420700001800440856006300458 2010 eng d a978051171225800aLife in Antarctic Deserts and Other Cold Environments0 aLife in Antarctic Deserts and Other Cold Environments aCambridgebCambridge University Press. a3070 v510aPlanetary Science1 aDoran, Peter, T.1 aLyons, Berry1 aMcKnight, Diane, M.1 aMcKnight, Diane, M.1 aGooseff, Michael, N.1 aTakacs-Vesbach, Cristina, D.1 aMikucki, Jill, A.1 aTranter, Martyn1 aSun, Henry, J uhttp://ebooks.cambridge.org/ebook.jsf?bid=CBO978051171225800474nas a2200109 4500008004100000245008300041210006900124260002700193100001400220700002400234856010600258 2010 eng d00aMicrobial interactions with dissolved organic matter in saline natural waters0 aMicrobial interactions with dissolved organic matter in saline n bUniversity of Colorado1 aCawley, K1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/microbial-interactions-dissolved-organic-matter-saline-natural-waters00662nas a2200181 4500008004100000245013300041210006900174260001200243300001400255490000800269100002400277700002200301700002000323700002100343700001800364700002700382856007100409 2010 eng d00aNew insights into the origin and evolution of Lake Vida, McMurdo Dry Valleys, Antarctica — A noble gas study in ice and brines0 aNew insights into the origin and evolution of Lake Vida McMurdo  c01/2010 a112 - 1220 v2891 aMalone, Jessica, L.1 aCastro, Clara, M.1 aHall, Chris, M.1 aDoran, Peter, T.1 aKenig, Fabien1 aMcKay, Christopher, P. uhttp://www.sciencedirect.com/science/article/pii/S0012821X0900633500684nas a2200169 4500008004100000245012300041210006900164260001100233300001400244490000700258100002800265700002400293700002900317700002100346700001800367856012900385 2010 eng d00aOrigin and tentative identification of tri to pentaunsaturated ketones in sediments from Lake Fryxell, East Antarctica0 aOrigin and tentative identification of tri to pentaunsaturated k c4/2010 a386 - 3970 v411 aJaraula, Caroline, M.B.1 aBrassell, Simon, C.1 aMorgan-Kiss, Rachael, M.1 aDoran, Peter, T.1 aKenig, Fabien uhttps://mcm.lternet.edu/content/origin-and-tentative-identification-tri-pentaunsaturated-ketones-sediments-lake-fryxell-east00643nas a2200169 4500008004100000245011300041210006900154260001200223300001400235490000700249100001800256700002100274700001800295700002100313700001900334856012000353 2010 eng d00aPalaeoenvironmental implications derived from a piston core from east lobe Bonney, Taylor Valley, Antarctica0 aPalaeoenvironmental implications derived from a piston core from c10/2010 a522 - 5300 v221 aWagner, Bernd1 aOrtlepp, Sabrina1 aKenig, Fabien1 aDoran, Peter, T.1 aMelles, Martin uhttps://mcm.lternet.edu/content/palaeoenvironmental-implications-derived-piston-core-east-lobe-bonney-taylor-valley00627nas a2200145 4500008004100000245010900041210006900150100001700219700001700236700002800253700002900281700002100310700002400331856012600355 2010 eng d00aPhysiochemical properties influencing biomass abundance and primary production in Lake Hoare, Antarctica0 aPhysiochemical properties influencing biomass abundance and prim1 aHerbei, Radu1 aLyons, Berry1 aLaybourn-Parry, Johanna1 aGardner, Christopher, B.1 aPriscu, John, C.1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/physiochemical-properties-influencing-biomass-abundance-and-primary-production-lake-hoare00649nas a2200169 4500008004100000020001800041245010800059210006900167260004200236300001400278100002200292700001700314700001500331700002200346700002100368856009000389 2010 eng d a978052188919300aSaline lakes and ponds in the McMurdo Dry Valleys: ecological analogs to martian paleolake environments0 aSaline lakes and ponds in the McMurdo Dry Valleys ecological ana aCambridgebCambridge University Press a160 - 1941 aMikucki, Jill, A.1 aLyons, Berry1 aHawes, Ian1 aLanoil, Brian, D.1 aDoran, Peter, T. uhttp://ebooks.cambridge.org/chapter.jsf?bid=CBO9780511712258&cid=CBO9780511712258A01301745nas a2200193 4500008004100000245004900041210004700090260001200137300001200149490000700161520118900168653001101357100002401368700002201392700002101414700002401435700002001459856007201479 2010 eng d00aSnow in the McMurdo Dry Valleys, Antarctica.0 aSnow in the McMurdo Dry Valleys Antarctica c04/2010 a633-6420 v303 a

Snowfall was measured at 11 sites in the McMurdo Dry Valleys to determine its magnitude, its temporal changes, and spatial patterns. Annual values ranged from 3 to 50 mm water equivalent with the highest values nearest the coast and decreasing inland. A particularly strong spatial gradient exists in Taylor Valley, probably resulting from local uplift conditions at the coastal margin and valley topography that limits migration inland. More snow occurs in winter near the coast, whereas inland no seasonal pattern is discernable. This may be due, again, to local uplift conditions, which are common in winter. We find no influence of the distance to the sea ice edge. Katabatic winds play an important role in transporting snow to the valley bottoms and essentially double the precipitation. That much of the snow accumulation sublimates prior to making a hydrologic contribution underscores the notion that the McMurdo Dry Valleys are indeed an extreme polar desert. Copyright © 2009 Royal Meteorological Society

10aBiggie1 aFountain, Andrew, G1 aNylen, Thomas, H.1 aMonaghan, Andrew1 aBasagic, Hassan, J.1 aBromwich, David uhttps://mcm.lternet.edu/content/snow-mcmurdo-dry-valleys-antarctica00685nas a2200193 4500008004100000245010500041210006900146260001200215300001400227490000700241100002400248700001700272700001900289700002900308700002500337700002400362700002100386856008400407 2010 eng d00aSpatial variations in the geochemistry of glacial meltwater streams in the Taylor Valley, Antarctica0 aSpatial variations in the geochemistry of glacial meltwater stre c12/2010 a662 - 6720 v221 aWelch, Kathleen, A.1 aLyons, Berry1 aWhisner, Carla1 aGardner, Christopher, B.1 aGooseff, Michael, N.1 aMcKnight, Diane, M.1 aPriscu, John, C. uhttp://www.montana.edu/lkbonney/DOCS/Publications/WelchEtAl2010Geochemistry.pdf00839nas a2200217 4500008004100000020002200041245013600063210007100199260003300270300001400303100001400317700002200331700001500353700002600368700001500394700002200409700002100431700001300452700002100465856013500486 2010 eng d a978-1-4244-5038-100aToward autonomous scientific exploration of ice-covered lakes—Field experiments with the ENDURANCE AUV in an Antarctic Dry Valley0 aToward autonomous scientific exploration of icecovered lakes—Fie aAnchorage, AKbIEEEc05/2010 a308 - 3151 aGulati, S1 aRichmond, Kristof1 aFlesher, C1 aHogan, Bartholomew, P1 aMurarka, A1 aKuhlmann, Gregory1 aSridharan, Mohan1 aStone, W1 aDoran, Peter, T. uhttps://mcm.lternet.edu/content/toward-autonomous-scientific-exploration-ice-covered-lakes%E2%80%94field-experiments-endurance-auv00918nas a2200325 4500008004100000245006400041210006300105300001200168490000700180100001700187700001600204700001400220700001600234700001500250700001400265700002500279700001500304700001300319700002000332700002600352700001600378700001700394700001900411700001300430700001500443700001200458700001500470700001500485856009200500 2009 eng d00aAccelerate Synthesis in Ecology and Environmental Sciences.0 aAccelerate Synthesis in Ecology and Environmental Sciences a699-7010 v591 aCarpenter, S1 aLundberg, P1 aMangel, M1 aMerchant, N1 aMurdoch, W1 aPalmer, M1 aPeters, Debra, P. C.1 aPickett, S1 aSmith, K1 aWall, Diana, H.1 aZimmerman, Maggie, K.1 aArmbrust, E1 aArzberger, E1 aChapin, F., S.1 aElser, J1 aHackett, E1 aIves, A1 aKareiva, P1 aLeibold, M uhttps://mcm.lternet.edu/content/accelerate-synthesis-ecology-and-environmental-sciences01755nas a2200241 4500008004100000245006900041210006500110260001200175300001200187490000800199520109500207653001101302100002201313700001501335700001601350700001501366700001601381700001401397700001301411700002101424700001101445856005701456 2009 eng d00aA Contemporary Microbially Maintained Subglacial Ferrous "Ocean"0 aContemporary Microbially Maintained Subglacial Ferrous Ocean c04/2009 a397-4000 v3243 a

An active microbial assemblage cycles sulfur in a sulfate-rich, ancient marine brine beneath Taylor Glacier, an outlet glacier of the East Antarctic Ice Sheet, with Fe(III) serving as the terminal electron acceptor. Isotopic measurements of sulfate, water, carbonate, and ferrous iron and functional gene analyses of adenosine 5′-phosphosulfate reductase imply that a microbial consortium facilitates a catalytic sulfur cycle. These metabolic pathways result from a limited organic carbon supply because of the absence of contemporary photosynthesis, yielding a subglacial ferrous brine that is anoxic but not sulfidic. Coupled biogeochemical processes below the glacier enable subglacial microbes to grow in extended isolation, demonstrating how analogous organic-starved systems, such as Neoproterozoic oceans, accumulated Fe(II) despite the presence of an active sulfur cycle.

10aBiggie1 aMikucki, Jill, A.1 aPearson, A1 aJohnston, D1 aTurchyn, A1 aFarquhar, J1 aSchrag, D1 aAnbar, A1 aPriscu, John, C.1 aLee, P uhttp://www.sciencemag.org/content/324/5925/397.short03833nas a2200217 4500008004100000245017200041210006900213260004600282300000800328490001000336520305700346653001503403653001903418653002103437653002503458653001903483653002303502100001603525700002403541856005003565 2009 eng d00aControls on stream and hyporheic temperatures, Taylor Valley, Antarctica and large-scale climate influences on interannual flow variation in the Onyx River, Antarctica0 aControls on stream and hyporheic temperatures Taylor Valley Anta aBoulder, CObUniversity of Coloradoc2009 a3170 vPh.D.3 a

The McMurdo Dry Valleys comprise the largest ice-free polar desert on the continent of Antarctica. My Ph.D. research investigated summertime glacial meltwater streams flowing through this region. This work is presented in Chapters 2 through 6 of my thesis. Chapters 2-5 present the work I have done related to hyporheic processes while Chapter 6 focuses on the hydroclimatological investigations I have carried out.

More specifically, Chapter 2 addresses the question: what are the dominant processes controlling dry valley stream temperatures? In particular, this investigation quantified the role of hyporheic exchange. The study found that in the Dry Valleys, exchange acted to decrease stream temperatures, accounting for 6–21% of cooling.

Chapter 3 discusses a follow up tracer study to investigate whether the comparatively large daily changes in dry valley stream temperatures (6-9°C) affect hyporheic processes, for instance through viscosity effects. Results showed that the hyporheic zone volume and exchange coefficient were lower during the warmer, afternoon stream/streambed temperature regime than during the cooler, morning one. A temperature-induced feedback mechanism that increases subsurface flow path preferentiality is proposed as a possible explanation for the reduction in hyporheic volume under warmer conditions. The tracer results also suggested a “Swiss Cheese” type conceptual model of the hyporheic zone in which flow takes place along paths weaving their way through isolated areas.

Chapter 4 presents work done to elucidate individual hyporheic flow path lengths and residence times. A streambed injection revealed some long (over 100 m) paths that were also fast, having subsurface travel times on par with the surface water. Hyporheic pipeflow is proposed as an explanation.

In Chapter 5 research is presented showing that nitrate and phosphate concentrations at specific locations in the hyporheic zone increase with the decreasing connectivity of that location to the stream.

Finally, Chapter 6 describes the large-scale climate conditions that prevailed during December and January during the highest and lowest flow summers of the Onyx River record, the longest flow record for Antarctica. Climate variables and regions in the Southern Hemisphere that had a statistically significant linear correlation to Onyx River flows were also identified. The highest flow summer on record, 2001-2, was found to have some unusual climate features when compared to the other high flow summers. It stands out as having an anomalous wind pattern that would have increased katabatic winds in the valleys, raising air temperatures and possibly depositing sediment on the glaciers, decreasing their albedo. It is also characterized by anomalously high incoming shortwave radiation. We postulate that those high levels may have been due in part to the unusually low concentrations of radiation absorbing stratospheric ozone prevalent over the valleys that particular summer.

10aAntarctica10aearth sciences10ahydroclimatology10ahyporheic flow paths10ahyporheic zone10astream temperature1 aCozzetto, K1 aMcKnight, Diane, M. uhttps://search.proquest.com/docview/30486636600675nas a2200193 4500008004100000245008100041210006900122260001200191300000700203490000600210100002400216700002700240700002400267700002000291700001700311700002200328700002200350856010900372 2009 eng d00aEnvironmental DNA sequencing primers for eutardigrades and bdelloid rotifers0 aEnvironmental DNA sequencing primers for eutardigrades and bdell c11/2009 a250 v91 aRobeson, Michael, S1 aCostello, Elizabeth, K1 aFreeman, Kristen, R1 aWhiting, Jeremy1 aAdams, Byron1 aMartin, Andrew, P1 aSchmidt, Steve, K uhttps://mcm.lternet.edu/content/environmental-dna-sequencing-primers-eutardigrades-and-bdelloid-rotifers01451nas a2200481 4500008004100000245006900041210006900110260001200179300001600191490001400207100002200221700002200243700002200265700002500287700002400312700002600336700001800362700001800380700002100398700002200419700002800441700001700469700001800486700002200504700002000526700002300546700002400569700002100593700001900614700002200633700001800655700002500673700001800698700002600716700002500742700002100767700002000788700002300808700002700831700002700858700002600885856005800911 2009 eng d00aLakes and reservoirs as regulators of carbon cycling and climate0 aLakes and reservoirs as regulators of carbon cycling and climate c11/2009 a2298–23140 v54 part 21 aTranvik, Lars, J.1 aDowning, John, A.1 aCotner, James, B.1 aLoiselle, Steven, A.1 aStriegl, Robert, G.1 aBallatore, Thomas, J.1 aDillon, Peter1 aFinlay, Kerri1 aFortino, Kenneth1 aKnoll, Lesley, B.1 aKortelainen, Pirkko, L.1 aKutser, Tiit1 aLarsen, Soren1 aLaurion, Isabelle1 aLeech, Dina, M.1 aMcCallister, Leigh1 aMcKnight, Diane, M.1 aMelack, John, M.1 aOverholt, Erin1 aPorter, Jason, A.1 aPrairie, Yves1 aRenwick, William, H.1 aRoland, Fabio1 aSherman, Bradford, S.1 aSchindler, David, W.1 aSobek, Sebastian1 aTremblay, Alain1 aVanni, Michael, J.1 aVerschoor, Antonie, M.1 avon Wachenfeldt, Eddie1 aWeyhenmeyer, Gesa, A. uhttp://aslo.org/lo/toc/vol_54/issue_6_part_2/2298.pdf00835nas a2200289 4500008004100000245006600041210006500107490000700172100002300179700002000202700001600222700001600238700001700254700001400271700001200285700001200297700001700309700001600326700001900342700001400361700001300375700002200388700001500410700001500425700001900440856008600459 2009 eng d00aState of the Antarctic and the Southern Ocean climate system,0 aState of the Antarctic and the Southern Ocean climate system0 v471 aMayewski, Paul, A.1 aBromwich, David1 aCampbell, H1 aHamilton, G1 aLyons, Berry1 aMaasch, K1 aAoki, S1 aXiao, C1 avan Ommen, T1 aMeredith, M1 aSummerhayes, C1 aTurner, J1 aWorby, A1 aBarrett, John, E.1 aCasassa, G1 aBertler, N1 aBracegirdle, T uhttps://mcm.lternet.edu/content/state-antarctic-and-southern-ocean-climate-system00526nas a2200169 4500008004100000245004000041210004000081260003000121300001000151100002500161700001600186700002100202700002000223700002700243700001700270856006900287 2008 eng d00aBacteria in Subglacial Environments0 aBacteria in Subglacial Environments aNew YorkbSpringer Verlag a51-711 aChristner, Brent, C.1 aSkidmore, M1 aPriscu, John, C.1 aTranter, Martyn1 aForeman, Christine, M.1 aMargesin, R. uhttps://mcm.lternet.edu/content/bacteria-subglacial-environments00639nas a2200169 4500008004100000245009800041210006900139300001200208490000600220100002100226700002700247700001900274700001800293700002700311700001200338856011900350 2008 eng d00aEntry approach into pristine ice-sealed lakes - Lake Vida, East Antarctica, a model ecosystem0 aEntry approach into pristine icesealed lakes Lake Vida East Anta a542-5470 v61 aDoran, Peter, T.1 aFritsen, Christian, H.1 aMurray, A., E.1 aKenig, Fabien1 aMcKay, Christopher, P.1 aKyne, J uhttps://mcm.lternet.edu/content/entry-approach-pristine-ice-sealed-lakes-lake-vida-east-antarctica-model-ecosystem01642nas a2200541 4500008004100000245010100041210006900142260001200211300001400223490000700237100002000244700002300264700002100287700002300308700002900331700002300360700002200383700002400405700001700429700002000446700002100466700002600487700002000513700002200533700002400555700002400579700001900603700002200622700002200644700002100666700001700687700002200704700002300726700001500749700001900764700002100783700002100804700002400825700002200849700002300871700002600894700002300920700001900943700002900962700001500991700001701006856007701023 2008 eng d00aGlobal decomposition experiment shows soil animal impacts on decomposition are climate dependent0 aGlobal decomposition experiment shows soil animal impacts on dec c11/2008 a2661-26770 v141 aWall, Diana, H.1 aBradford, Mark, A.1 aStJohn, Mark, G.1 aTrofymow, John, A.1 aBehan-Pelletier, Valerie1 aBignell, David, E.1 aDangerfield, Mark1 aParton, William, J.1 aRusek, Josef1 aVoigt, Winfried1 aWolters, Volkmar1 aGardel, Holley, Zadeh1 aAyuke, Fred, O.1 aBashford, Richard1 aBeljakova, Olga, I.1 aBohlen, Patrick, J.1 aBrauman, Alain1 aFlemming, Stephen1 aHenschel, Joh, R.1 aJohnson, Dan, L.1 aJones, Hefin1 aKovarova, Marcela1 aKranabetter, Marty1 aKutny, Les1 aLin, Kuo-Chuan1 aMaryati, Mohamed1 aMasse, Dominique1 aPokarzhevsk, Andrei1 aRahman, Homathevi1 aSabara, Millor, G.1 aSalamon, Joerg-Alfred1 aSwift, Michael, J.1 aVarela, Amanda1 aVasconcelos, Heraldo, L.1 aWhite, Don1 aZou, Xiaming uhttp://onlinelibrary.wiley.com/doi/10.1111/j.1365-2486.2008.01672.x/full00521nas a2200157 4500008004100000245003800041210003600079260003600115100002400151700002500175700002500200700001600225700002500241700002800266856006900294 2008 eng d00aHigh-latitude rivers and streams.0 aHighlatitude rivers and streams aOxfordbOxford University Press1 aMcKnight, Diane, M.1 aGooseff, Michael, N.1 aVincent, Warwick, F.1 aPeterson, B1 aVincent, Warwick, F.1 aLaybourn-Parry, Johanna uhttps://mcm.lternet.edu/content/high-latitude-rivers-and-streams00775nas a2200217 4500008004100000245010100041210006900142260001500211300001200226490000700238653002100245653001200266100002100278700002700299700002400326700002200350700002400372700001700396700002200413856012200435 2008 eng d00aHydrologic response to extreme warm and cold summers in the McMurdo Dry Valleys, East Antarctica0 aHydrologic response to extreme warm and cold summers in the McMu c05/16/2008 a499-5090 v2010aClimate Response10astreams1 aDoran, Peter, T.1 aMcKay, Christopher, P.1 aFountain, Andrew, G1 aNylen, Thomas, H.1 aMcKnight, Diane, M.1 aJaros, Chris1 aBarrett, John, E. uhttps://mcm.lternet.edu/content/hydrologic-response-extreme-warm-and-cold-summers-mcmurdo-dry-valleys-east-antarctica00672nas a2200193 4500008004100000245008200041210006900123300001400192490000700206100002500213700002500238700002400263700001700287700001600304700001600320700002100336700001700357856010400374 2008 eng d00aInland diatoms from the McMurdo Dry Valleys and James Ross Island, Antarctica0 aInland diatoms from the McMurdo Dry Valleys and James Ross Islan a1378-13920 v121 aEsposito, Rhea, M.M.1 aSpaulding, Sarah, A.1 aMcKnight, Diane, M.1 aDe Vijver, B1 aKopalova, K1 aLubinski, D1 aHall, Brenda, L.1 aWhittaker, T uhttps://mcm.lternet.edu/content/inland-diatoms-mcmurdo-dry-valleys-and-james-ross-island-antarctica00626nas a2200181 4500008004100000245008300041210006900124300001400193490000700207100002000214700001600234700001400250700001100264700002200275700002000297700001900317856010800336 2008 eng d00aMicrobial community composition in soils of Northern Victoria Land, Antarctica0 aMicrobial community composition in soils of Northern Victoria La a1713-17240 v101 aNiederberger, T1 aMcDonald, i1 aHacker, A1 aSoo, R1 aBarrett, John, E.1 aWall, Diana, H.1 aCary, Craig, S uhttps://mcm.lternet.edu/content/microbial-community-composition-soils-northern-victoria-land-antarctica00530nas a2200121 4500008004100000245011000041210006900151300001400220490000700234100002200241700002100263856012400284 2007 eng d00aBacterial diversity associated with Blood Falls, A subglacial outflow from the Taylor Glacier, Antarctica0 aBacterial diversity associated with Blood Falls A subglacial out a4029-40390 v731 aMikucki, Jill, A.1 aPriscu, John, C. uhttps://mcm.lternet.edu/content/bacterial-diversity-associated-blood-falls-subglacial-outflow-taylor-glacier-antarctica03147nas a2200241 4500008004100000245006800041210006800109260001200177300001400189490000800203520239000211653001102601100002202612700002302634700001702657700002402674700002102698700002402719700002002743700002402763700002102787856009702808 2007 eng d00aBiogeochemical stoichiometry of Antarctic Dry Valley ecosystems0 aBiogeochemical stoichiometry of Antarctic Dry Valley ecosystems c02/2007 aG01010+120 v1123 a

Among aquatic and terrestrial landscapes of the McMurdo Dry Valleys, Antarctica, ecosystem stoichiometry ranges from values near the Redfield ratios for C:N:P to nutrient concentrations in proportions far above or below ratios necessary to support balanced microbial growth. This polar desert provides an opportunity to evaluate stoichiometric approaches to understand nutrient cycling in an ecosystem where biological diversity and activity are low, and controls over the movement and mass balances of nutrients operate over 10–106 years. The simple organisms (microbial and metazoan) comprising dry valley foodwebs adhere to strict biochemical requirements in the composition of their biomass, and when activated by availability of liquid water, they influence the chemical composition of their environment according to these ratios. Nitrogen and phosphorus varied significantly in terrestrial and aquatic ecosystems occurring on landscape surfaces across a wide range of exposure ages, indicating strong influences of landscape development and geochemistry on nutrient availability. Biota control the elemental ratio of stream waters, while geochemical stoichiometry (e.g., weathering, atmospheric deposition) evidently limits the distribution of soil invertebrates. We present a conceptual model describing transformations across dry valley landscapes facilitated by exchanges of liquid water and biotic processing of dissolved nutrients. We conclude that contemporary ecosystem stoichiometry of Antarctic Dry Valley soils, glaciers, streams, and lakes results from a combination of extant biological processes superimposed on a legacy of landscape processes and previous climates.

10aBiggie1 aBarrett, John, E.1 aVirginia, Ross, A.1 aLyons, Berry1 aMcKnight, Diane, M.1 aPriscu, John, C.1 aFountain, Andrew, G1 aWall, Diana, H.1 aMoorhead, Daryl, L.1 aDoran, Peter, T. uhttps://mcm.lternet.edu/content/biogeochemical-stoichiometry-antarctic-dry-valley-ecosystems00503nas a2200097 4500008004100000245013700041210006900178300001000247100002400257856012400281 2007 eng d00aMesoscale dynamics of ephemeral wetlands in the Antarctic Dry Valleys: Implications to production and distribution of organic matter0 aMesoscale dynamics of ephemeral wetlands in the Antarctic Dry Va a87-951 aMoorhead, Daryl, L. uhttps://mcm.lternet.edu/content/mesoscale-dynamics-ephemeral-wetlands-antarctic-dry-valleys-implications-production-and00558nas a2200145 4500008004100000245008500041210006900126490000800195100002700203700001500230700002200245700001800267700002100285856010600306 2007 eng d00aMetabolic activity and diversity of cryoconites in the Taylor Valley, Antarctica0 aMetabolic activity and diversity of cryoconites in the Taylor Va0 v1121 aForeman, Christine, M.1 aSattler, B1 aMikucki, Jill, A.1 aPorazinska, D1 aPriscu, John, C. uhttps://mcm.lternet.edu/content/metabolic-activity-and-diversity-cryoconites-taylor-valley-antarctica00476nas a2200121 4500008004100000245007900041210006900120260002900189490000900218100001300227700002100240856009300261 2007 eng d00aMicrobial Processes in the Moats of Lakes in the Taylor Valley, Antarctica0 aMicrobial Processes in the Moats of Lakes in the Taylor Valley A bMontana State University0 vM.S.1 aMoore, J1 aPriscu, John, C. uhttps://mcm.lternet.edu/content/microbial-processes-moats-lakes-taylor-valley-antarctica00763nas a2200205 4500008004100000245013200041210006900173260001200242300001200254490000700266100002400273700002000297700002400317700002000341700001600361700002400377700001700401700001900418856012000437 2007 eng d00aReactivation of a cryptobiotic stream ecosystem in the McMurdo Dry Valleys, Antarctica: A long-term geomorphological experiment0 aReactivation of a cryptobiotic stream ecosystem in the McMurdo D c09/2007 a186-2040 v891 aMcKnight, Diane, M.1 aTate, Cathy, M.1 aAndrews, Edmund, D.1 aNiyogi, Dev, K.1 aCozzetto, K1 aWelch, Kathleen, A.1 aLyons, Berry1 aCapone, D., G. uhttps://mcm.lternet.edu/content/reactivation-cryptobiotic-stream-ecosystem-mcmurdo-dry-valleys-antarctica-long-term00498nas a2200121 4500008004100000245007800041210006900119260003600188490000900224100002100233700002100254856010100275 2007 eng d00aStable Carbon Isotope Analyses of Sediments from Lake Fryxell, Antarctica0 aStable Carbon Isotope Analyses of Sediments from Lake Fryxell An bUniversity of Illinois, Chicago0 vM.S.1 aMoore-Topinka, N1 aDoran, Peter, T. uhttps://mcm.lternet.edu/content/stable-carbon-isotope-analyses-sediments-lake-fryxell-antarctica00704nas a2200169 4500008004100000245010300041210006900144260005400213100002500267700002400292700002100316700001700337700001700354700001900371700002700390856011700417 2007 eng d00aTrends in discharge and flow season timing of the Onyx River, Wright Valley, Antarctica since 19690 aTrends in discharge and flow season timing of the Onyx River Wri bU.S. Geological Survey Open-File Report 2007-10471 aGooseff, Michael, N.1 aMcKnight, Diane, M.1 aDoran, Peter, T.1 aLyons, Berry1 aCooper, Alan1 aRaymond, Carol1 aTeam, ISAES, Editorial uhttps://mcm.lternet.edu/content/trends-discharge-and-flow-season-timing-onyx-river-wright-valley-antarctica-196902534nas a2200193 4500008004100000245009700041210006900138260001200207300001200219490000700231520185500238653001102093100002902104700002102133700001402154700002502168700002402193856012302217 2006 eng d00aAdaptation and acclimation of photosynthetic microorganisms to permanently cold environments0 aAdaptation and acclimation of photosynthetic microorganisms to p c03/2006 a222-2520 v703 a

Persistently cold environments constitute one of our world's largest ecosystems, and microorganisms dominate the biomass and metabolic activity in these extreme environments. The stress of low temperatures on life is exacerbated in organisms that rely on photoautrophic production of organic carbon and energy sources. Phototrophic organisms must coordinate temperature-independent reactions of light absorption and photochemistry with temperature-dependent processes of electron transport and utilization of energy sources through growth and metabolism. Despite this conundrum, phototrophic microorganisms thrive in all cold ecosystems described and (together with chemoautrophs) provide the base of autotrophic production in low-temperature food webs. Psychrophilic (organisms with a requirement for low growth temperatures) and psychrotolerant (organisms tolerant of low growth temperatures) photoautotrophs rely on low-temperature acclimative and adaptive strategies that have been described for other low-temperature-adapted heterotrophic organisms, such as cold-active proteins and maintenance of membrane fluidity. In addition, photoautrophic organisms possess other strategies to balance the absorption of light and the transduction of light energy to stored chemical energy products (NADPH and ATP) with downstream consumption of photosynthetically derived energy products at low temperatures. Lastly, differential adaptive and acclimative mechanisms exist in phototrophic microorganisms residing in low-temperature environments that are exposed to constant low-light environments versus high-light- and high-UV-exposed phototrophic assemblages.

10aBiggie1 aMorgan-Kiss, Rachael, M.1 aPriscu, John, C.1 aPocock, T1 aGudynaite-Savitch, L1 aHuner, Norman, P.A. uhttps://mcm.lternet.edu/content/adaptation-and-acclimation-photosynthetic-microorganisms-permanently-cold-environments00754nas a2200205 4500008004100000245012400041210006900165300001200234490000700246100001600253700001500269700002400284700001700308700002300325700001300348700002200361700001500383700002400398856012600422 2006 eng d00aThe aeolian flux of calcium, chloride and nitrate to the McMurdo Dry Valleys landscape: Evidence from snow pit analysis0 aaeolian flux of calcium chloride and nitrate to the McMurdo Dry  a497-5050 v181 aWitherow, R1 aBertler, N1 aWelch, Kathleen, A.1 aLyons, Berry1 aMayewski, Paul, A.1 aSneed, S1 aNylen, Thomas, H.1 aHandley, M1 aFountain, Andrew, G uhttps://mcm.lternet.edu/content/aeolian-flux-calcium-chloride-and-nitrate-mcmurdo-dry-valleys-landscape-evidence-snow-pit01738nas a2200229 4500008004100000245008300041210006900124260001200193300001100205490000700216520101800223653001101241100002501252700001201277700002401289700001101313700001301324700002501337700002101362700001601383856010901399 2006 eng d00aAntarctic Climate Cooling and Response of Diatoms in Glacial Meltwater Streams0 aAntarctic Climate Cooling and Response of Diatoms in Glacial Mel c04/2006 aL074060 v333 a

To understand biotic responses to an Antarctic cooling trend, we analyzed diatom samples from glacial meltwater streams in the McMurdo Dry Valleys, the largest ice-free area in Antarctica. Diatoms are abundant in these streams, and 24 of 40 species have only been found in the Antarctic. The percentage of these Antarctic diatom species increased with decreasing annual stream flow and increasing harshness of the stream habitat. The species diversity of assemblages reached a maximum when the Antarctic species accounted for 40–60% of relative diatom abundance. Decreased solar radiation and air-temperatures reduce annual stream flow, raising the dominance of these Antarctic species to levels above 60%. Thus, cooling favors the Antarctic species, and lowers diatom species diversity in this region.

10aBiggie1 aEsposito, Rhea, M.M.1 aHorn, S1 aMcKnight, Diane, M.1 aCox, M1 aGrant, M1 aSpaulding, Sarah, A.1 aDoran, Peter, T.1 aCozzetto, K uhttps://mcm.lternet.edu/content/antarctic-climate-cooling-and-response-diatoms-glacial-meltwater-streams00600nas a2200145 4500008004100000245011900041210006900160300001200229490000700241100001500248700002400263700001500287700002700302856012500329 2006 eng d00aEnvironmental factors associated with deep chlorophyll maxima in dry valley lakes, South Victoria Land, Antarctica0 aEnvironmental factors associated with deep chlorophyll maxima in a179-1890 v381 aBurnett, L1 aMoorhead, Daryl, L.1 aHawes, Ian1 aHoward-Williams, Clive uhttps://mcm.lternet.edu/content/environmental-factors-associated-deep-chlorophyll-maxima-dry-valley-lakes-south-victoria00609nas a2200145 4500008004100000245012000041210006900161300001200230490000700242100001600249700002400265700002200289700002400311856012800335 2006 eng d00aExperimental investigations into processes controlling stream and hyporheic temperatures, Fryxell Basin, Antarctica0 aExperimental investigations into processes controlling stream an a130-1530 v291 aCozzetto, K1 aMcKnight, Diane, M.1 aNylen, Thomas, H.1 aFountain, Andrew, G uhttps://mcm.lternet.edu/content/experimental-investigations-processes-controlling-stream-and-hyporheic-temperatures-fryxell00655nas a2200181 4500008004100000245011400041210006900155300000800224490000800232100001400240700001400254700002100268700001800289700001400307700001400321700001300335856012500348 2006 eng d00aGlacial and postglacial sedimentation in the Fryxell basin, Taylor Valley, Southern Victoria Land, Antarctica0 aGlacial and postglacial sedimentation in the Fryxell basin Taylo a3200 v3411 aWagner, B1 aMelles, M1 aDoran, Peter, T.1 aKenig, Fabien1 aForman, S1 aPierau, R1 aAllan, P uhttps://mcm.lternet.edu/content/glacial-and-postglacial-sedimentation-fryxell-basin-taylor-valley-southern-victoria-land00551nas a2200145 4500008004100000245008500041210006900126300000800195490000700203100002400210700002200234700001600256700002000272856011300292 2006 eng d00aGlacier mass balances (1993-2001) Taylor Valley, McMurdo Dry Valleys, Antarctica0 aGlacier mass balances 19932001 Taylor Valley McMurdo Dry Valleys a4510 v521 aFountain, Andrew, G1 aNylen, Thomas, H.1 aMacClune, K1 aDana, Gayle, L. uhttps://mcm.lternet.edu/content/glacier-mass-balances-1993-2001-taylor-valley-mcmurdo-dry-valleys-antarctica00331nam a2200121 4500008004100000020001900041245001800060210001400078260004000092300000700132100002400139856004600163 2006 eng d a978-097234227800aThe Lost Seal0 aLost Seal aLafayettebMoonlight Publishing LLC a401 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/lost-seal00487nas a2200109 4500008004100000245009400041210006900135260003000204100001400234700001700248856011200265 2006 eng d00aA Qualitative Approach to Understanding the Rate of Weathering, Taylor Valley, Antarctica0 aQualitative Approach to Understanding the Rate of Weathering Tay bThe Ohio State University1 aMiller, E1 aLyons, Berry uhttps://mcm.lternet.edu/content/qualitative-approach-understanding-rate-weathering-taylor-valley-antarctica00556nas a2200145 4500008004100000245008200041210006900123300001200192490000800204100001500212700002400227700002300251700002800274856010800302 2006 eng d00aSimulating energy flow through a pelagic food web in Lake Fryxell, Antarctica0 aSimulating energy flow through a pelagic food web in Lake Fryxel a457-4720 v1921 aMcKenna, K1 aMoorhead, Daryl, L.1 aRoberts, Emily, C.1 aLaybourn-Parry, Johanna uhttps://mcm.lternet.edu/content/simulating-energy-flow-through-pelagic-food-web-lake-fryxell-antarctica00585nas a2200133 4500008004100000245012400041210006900165300001000234490000700244100002400251700002300275700002400298856012900322 2006 eng d00aSpatial and temporal active layer dynamics along three glacial meltwater streams in the McMurdo Dry Valleys, Antarctica0 aSpatial and temporal active layer dynamics along three glacial m a42-530 v381 aConovitz, Peter, A.1 aMacDonald, Lee, H.1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/spatial-and-temporal-active-layer-dynamics-along-three-glacial-meltwater-streams-mcmurdo-dry00687nas a2200181 4500008004100000245010500041210006900146260001200215300001000227490000700237100002500244700001700269700002400286700002200310700002400332700002100356856012800377 2006 eng d00aA stable isotopic investigation of a polar desert hydrologic system, McMurdo Dry Valleys, Antarctica0 astable isotopic investigation of a polar desert hydrologic syste c02/2006 a60-710 v381 aGooseff, Michael, N.1 aLyons, Berry1 aMcKnight, Diane, M.1 aVaughn, Bruce, H.1 aFountain, Andrew, G1 aDowling, Carolyn uhttps://mcm.lternet.edu/content/stable-isotopic-investigation-polar-desert-hydrologic-system-mcmurdo-dry-valleys-antarctica00812nas a2200265 4500008004100000245006500041210006400106300001400170490000700184100002200191700002200213700001500235700001600250700002000266700001900286700002300305700002000328700001600348700001600364700001600380700001600396700001700412700002400429856009300453 2006 eng d00aTerrestrial ecosystem processes of Victoria Land, Antarctica0 aTerrestrial ecosystem processes of Victoria Land Antarctica a3019-30340 v381 aBarrett, John, E.1 aNkem, Johnson, N.1 aSletten, R1 aSteltzer, H1 aWall, Diana, H.1 aWallenstein, M1 aVirginia, Ross, A.1 aHopkins, D., W.1 aAislabie, J1 aBargagli, R1 aBockheim, J1 aCampbell, I1 aLyons, Berry1 aMoorhead, Daryl, L. uhttps://mcm.lternet.edu/content/terrestrial-ecosystem-processes-victoria-land-antarctica00559nas a2200157 4500008004100000245008000041210006900121490000700190100001300197700001400210700001500224700001500239700002100254700001700275856010900292 2005 eng d00aBiogeological Raman spectroscopic studies of Antarctic lacustrine sediments0 aBiogeological Raman spectroscopic studies of Antarctic lacustrin0 v611 aMoody, C1 aVillar, S1 aEdwards, H1 aHodgson, D1 aDoran, Peter, T.1 aBishop, J.L. uhttps://mcm.lternet.edu/content/biogeological-raman-spectroscopic-studies-antarctic-lacustrine-sediments00884nas a2200265 4500008004100000245007800041210007000119300001100189490000700200653002500207100002100232700001900253700002700272700002700299700002400326700002100350700001700371700001300388700002400401700002400425700002400449700002300473700002000496856010200516 2005 eng d00aComment on ``El Niño suppresses Antarctic warming'' by N. Bertler et al.0 aComment on El Niño suppresses Antarctic warming by N Bertler et  aL077060 v3210aTropical meteorology1 aDoran, Peter, T.1 aClow, Gary, D.1 aFritsen, Christian, H.1 aMcKay, Christopher, P.1 aParsons, Andrew, N.1 aPriscu, John, C.1 aLyons, Berry1 aWalsh, J1 aFountain, Andrew, G1 aMcKnight, Diane, M.1 aMoorhead, Daryl, L.1 aVirginia, Ross, A.1 aWall, Diana, H. uhttps://mcm.lternet.edu/content/comment-el-ni%C3%B1o-suppresses-antarctic-warming-n-bertler-et-al00509nas a2200121 4500008004100000245009600041210006900137490000700206100002400213700001700237700001500254856011800269 2005 eng d00aContributions of Benthic Microbial Mats to Net Primary Production in Lake Hoare, Antarctica0 aContributions of Benthic Microbial Mats to Net Primary Productio0 v171 aMoorhead, Daryl, L.1 aSchmeling, S1 aHawes, Ian uhttps://mcm.lternet.edu/content/contributions-benthic-microbial-mats-net-primary-production-lake-hoare-antarctica00549nas a2200157 4500008004100000245009600041210006900137300001200206490000800218100002500226700002200251700002700273700001400300700002100314856005600335 2005 eng d00aGlacial ice cores: a model system for developing extraterrestrial decontamination protocols0 aGlacial ice cores a model system for developing extraterrestrial a572-5840 v1741 aChristner, Brent, C.1 aMikucki, Jill, A.1 aForeman, Christine, M.1 aDenson, J1 aPriscu, John, C. u/reports/lakes/ChristnerEtAl2005Decontamination.pdf00681nas a2200181 4500008004100000245012100041210006900162300001200231490000700243100001700250700002400267700001400291700001400305700001400319700001900333700002300352856012400375 2005 eng d00aHalogen geochemistry of the McMurdo Dry Valleys Lakes, Antarctica: clues to the origin of solutes and lake evolution0 aHalogen geochemistry of the McMurdo Dry Valleys Lakes Antarctica a305-3230 v691 aLyons, Berry1 aWelch, Kathleen, A.1 aSnyder, G1 aOlesik, J1 aGraham, E1 aMarion, G., M.1 aPoreda, Robert, J. uhttps://mcm.lternet.edu/content/halogen-geochemistry-mcmurdo-dry-valleys-lakes-antarctica-clues-origin-solutes-and-lake00454nas a2200121 4500008004100000245006100041210006100102260002900163490001000192100002200202700002100224856008700245 2005 eng d00aMicrobial Ecology of an Antarctic Subglacial Environment0 aMicrobial Ecology of an Antarctic Subglacial Environment bMontana State University0 vPh.D.1 aMikucki, Jill, A.1 aPriscu, John, C. uhttps://mcm.lternet.edu/content/microbial-ecology-antarctic-subglacial-environment00755nas a2200217 4500008004100000245008700041210006900128260003100197300001000228100002100238700002200259700002000281700002700301700001900328700002000347700002000367700002200387700001900409700001700428856009200445 2005 eng d00aPerennial Antarctic lake ice: A refuge for cyanobacteria in an extreme environment0 aPerennial Antarctic lake ice A refuge for cyanobacteria in an ex bPrinceton University Press a22-491 aPriscu, John, C.1 aAdams, Edward, E.1 aPaerl, Hans, W.1 aFritsen, Christian, H.1 aDore, John, E.1 aLisle, John, T.1 aWolf, Craig, F.1 aMikucki, Jill, A.1 aRogers, S., O.1 aCastello, J. uhttp://www.montana.edu/lkbonney/DOCS/Publications/PriscuEtAl2005CyanobacteriaRefuge.pdf00662nas a2200193 4500008004100000245008500041210006900126260004000195300001200235100002700247700002300274700001500297700001600312700002100328700002700349700002100376700002000397856005100417 2005 eng d00aPolar lakes, streams, and springs as analogs for the hydrological cycle on Mars.0 aPolar lakes streams and springs as analogs for the hydrological  aBerlin, HeidelbergbSpringer Verlag a219-2331 aMcKay, Christopher, P.1 aAndersen, Dale, T.1 aPollard, W1 aHeldmann, J1 aDoran, Peter, T.1 aFritsen, Christian, H.1 aPriscu, John, C.1 aTokano, Tetsuya u/reports/lakes/McKayEtAl2005StreamsSprings.pdf00875nas a2200361 4500008004100000245001800041210001800059260001700077300001200094100001900106700001500125700001500140700002300155700001300178700001300191700001900204700001500223700001400238700002000252700001500272700001400287700001700301700001800318700001900336700001400355700002200369700001400391700001500405700001500420700001600435700001200451856005000463 2005 eng d00aPolar Systems0 aPolar Systems bIsland Press a717-7431 aChapin, F., S.1 aMcGuire, A1 aNuttall, M1 aVirginia, Ross, A.1 aYoung, O1 aZimov, S1 aChristensen, T1 aGodduhn, A1 aMurphy, E1 aWall, Diana, H.1 aZockler, C1 aBerman, M1 aCallaghan, T1 aConvey, Peter1 aCrepin, A., S.1 aDanell, K1 aDucklow, Hugh, W.1 aForbes, B1 aKofinas, G1 aHassan, R.1 aScholes, R.1 aAsh, N. uhttps://mcm.lternet.edu/content/polar-systems00700nas a2200169 4500008004100000245014000041210006900181260001200250300001200262490000700274100002500281700002500306700002300331700002300354700002400377856012900401 2005 eng d00aSensitivity analysis of conservative and reactive 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V.1 aSmol, J.P. uhttps://mcm.lternet.edu/content/geochronology-high-latitude-lake-sediments00629nas a2200157 4500008004100000245011500041210006900156300001200225490000700237100002200244700002700266700001500293700001700308700002100325856012500346 2004 eng d00aGeomicrobiology of Blood Fall: An iron-rich saline discharge at the terminus of the Taylor Glacier, Antarctica0 aGeomicrobiology of Blood Fall An ironrich saline discharge at th a199-2000 v101 aMikucki, Jill, A.1 aForeman, Christine, M.1 aSattler, B1 aLyons, Berry1 aPriscu, John, C. uhttps://mcm.lternet.edu/content/geomicrobiology-blood-fall-iron-rich-saline-discharge-terminus-taylor-glacier-antarctica00455nas a2200121 4500008004100000245007100041210006900112300001000181490000700191100002300198700001500221856009700236 2004 eng d00aGradient analysis of cryoconite ecosystems from two Polar glaciers0 aGradient analysis of cryoconite ecosystems from two Polar glacie a66-740 v271 aMueller, Derek, R.1 aPollard, W uhttps://mcm.lternet.edu/content/gradient-analysis-cryoconite-ecosystems-two-polar-glaciers-000555nas a2200121 4500008004100000245014000041210006900181300001200250490000700262100001400269700002400283856012600307 2004 eng d00aThe impact of anhydrobiosis on the persistence of Scottnema lindsyae (Nematoda): a modeling analysis of population stability thresholds0 aimpact of anhydrobiosis on the persistence of Scottnema lindsyae a507-5120 v271 aWeicht, T1 aMoorhead, Daryl, L. uhttps://mcm.lternet.edu/content/impact-anhydrobiosis-persistence-scottnema-lindsyae-nematoda-modeling-analysis-population00681nas a2200157 4500008004100000245015800041210006900199300001200268490000700280100002400287700002300311700001900334700002000353700002400373856012600397 2004 eng d00aInorganic nitrogen and phosphorus dynamics of Antarctic glacial meltwater streams as controlled by hyporheic exchange and benthic autotrophic communities0 aInorganic nitrogen and phosphorus dynamics of Antarctic glacial  a171-1880 v231 aMcKnight, Diane, M.1 aRunkel, Robert, L.1 aDuff, John, H.1 aTate, Cathy, M.1 aMoorhead, Daryl, L. uhttps://mcm.lternet.edu/content/inorganic-nitrogen-and-phosphorus-dynamics-antarctic-glacial-meltwater-streams-controlled00568nas a2200133 4500008004100000245010500041210006900146300001200215490000700227100002500234700002400259700002300283856012800306 2004 eng d00aReach-scale cation exchange controls on major ion chemistry of an Antarctic glacial meltwater stream0 aReachscale cation exchange controls on major ion chemistry of an a221-2380 v101 aGooseff, Michael, N.1 aMcKnight, Diane, M.1 aRunkel, Robert, L. uhttps://mcm.lternet.edu/content/reach-scale-cation-exchange-controls-major-ion-chemistry-antarctic-glacial-meltwater-stream00726nas a2200205 4500008004100000245011200041210006900153300000900222490000700231100001100238700002200249700002700271700002100298700001900319700001500338700001400353700002000367700001400387856011900401 2004 eng d00aThermodynamic constraints on microbially mediated processes in lakes of the McMurdo Dry Valleys, Antarctica0 aThermodynamic constraints on microbially mediated processes in l a1-170 v211 aLee, P1 aMikucki, Jill, A.1 aForeman, Christine, M.1 aPriscu, John, C.1 aDiTullio, G, R1 aRiseman, S1 aDeMora, S1 aWolf, Craig, F.1 aKester, L uhttps://mcm.lternet.edu/content/thermodynamic-constraints-microbially-mediated-processes-lakes-mcmurdo-dry-valleys00681nas a2200205 4500008004100000245009300041210006900134300001200203490000600215100001600221700001700237700001500254700001500269700001300284700001200297700001400309700001300323700002000336856011900356 2004 eng d00aTrophic interactions in a changing world: modelling aboveground-belowground interactions0 aTrophic interactions in a changing world modelling abovegroundbe a515-5280 v51 aSchroter, D1 aBrussaard, L1 aDe Deyn, G1 aProveda, K1 aBrown, V1 aBerg, M1 aWardle, D1 aMoore, J1 aWall, Diana, H. uhttps://mcm.lternet.edu/content/trophic-interactions-changing-world-modelling-aboveground-belowground-interactions00536nas a2200157 4500008004100000245006700041210006600108300001200174490000600186100001200192700002400204700001700228700002100245700002400266856008800290 2003 eng d00aBiochemistry of Si in the McMurdo Dry Valley lakes, Antarctica0 aBiochemistry of Si in the McMurdo Dry Valley lakes Antarctica a737-7490 v11 aPugh, H1 aWelch, Kathleen, A.1 aLyons, Berry1 aPriscu, John, C.1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/biochemistry-si-mcmurdo-dry-valley-lakes-antarctica00679nas a2200169 4500008004100000245011200041210006900153260001700222100002400239700001900263700001700282700002400299700002700323700001700350700001500367856012700382 2003 eng d00aChemistry and lake dynamics of the Taylor Valley lakes, Antarctica: The importance of long-term monitoring.0 aChemistry and lake dynamics of the Taylor Valley lakes Antarctic bCaxton Press1 aWelch, Kathleen, A.1 aNeumann, Klaus1 aLyons, Berry1 aMcKnight, Diane, M.1 aHoward-Williams, Clive1 aDavidson, W.1 aBroady, P. uhttps://mcm.lternet.edu/content/chemistry-and-lake-dynamics-taylor-valley-lakes-antarctica-importance-long-term-monitoring00558nas a2200145 4500008004100000245007900041210006900120300001400189490000700203100002500210700002400235700002300259700002200282856010800304 2003 eng d00aDetermining long time-scale hyporheic zone flow paths in Antarctic streams0 aDetermining long timescale hyporheic zone flow paths in Antarcti a1691-17100 v171 aGooseff, Michael, N.1 aMcKnight, Diane, M.1 aRunkel, Robert, L.1 aVaughn, Bruce, H. uhttps://mcm.lternet.edu/content/determining-long-time-scale-hyporheic-zone-flow-paths-antarctic-streams00567nas a2200169 4500008004100000245009200041210006900133260001200202300001000214490000800224100002100232700002700253700002700280700002100307700002200328856004700350 2003 eng d00aFormation and character of an ancient 19 m ice cover and underlying trapped brine in an0 aFormation and character of an ancient 19 m ice cover and underly c01/2003 a26-310 v1001 aDoran, Peter, T.1 aFritsen, Christian, H.1 aMcKay, Christopher, P.1 aPriscu, John, C.1 aAdams, Edward, E. uhttp://www.pnas.org/content/100/1/26.short00548nas a2200145 4500008004100000245009000041210006900131300000900200490000800209100002100217700002400238700001400262700001600276856011000292 2003 eng d00aGlaciers of the McMurdo Dry Valleys: terrestrial analog for Martian polar sublimation0 aGlaciers of the McMurdo Dry Valleys terrestrial analog for Marti a50310 v1081 aLewis, Karen, J.1 aFountain, Andrew, G1 aKargel, J1 aMacAyeal, D uhttps://mcm.lternet.edu/content/glaciers-mcmurdo-dry-valleys-terrestrial-analog-martian-polar-sublimation00584nas a2200181 4500008004100000245006300041210006000104300001000164100001500174700001600189700002000205700001300225700001600238700001800254700002500272700001400297856009100311 2003 eng d00aLong-term perspectives on biodiversity-ecosystem function.0 aLongterm perspectives on biodiversityecosystem function a89-981 aSymstad, A1 aIII, Chapin1 aWall, Diana, H.1 aGross, K1 aHuenneke, L1 aMittelbach, G1 aPeters, Debra, P. C.1 aTilman, D uhttps://mcm.lternet.edu/content/long-term-perspectives-biodiversity-ecosystem-function00585nas a2200157 4500008004100000245008200041210006900123300001400192490000700206100002400213700002200237700002300259700002000282700001800302856010700320 2003 eng d00aOrganic matter and soil biota of upland wetlands in Taylor Valley, Antarctica0 aOrganic matter and soil biota of upland wetlands in Taylor Valle a1009-10190 v261 aMoorhead, Daryl, L.1 aBarrett, John, E.1 aVirginia, Ross, A.1 aWall, Diana, H.1 aPorazinska, D uhttps://mcm.lternet.edu/content/organic-matter-and-soil-biota-upland-wetlands-taylor-valley-antarctica00682nas a2200169 4500008004100000245012300041210006900164300001200233490000700245100001700252700002400269700002400293700002000317700002200337700002400359856012900383 2003 eng d00aSurface glaciochemistry of Taylor Valley, southern Victoria Land, Antarctica and its relationship to stream chemistry.0 aSurface glaciochemistry of Taylor Valley southern Victoria Land  a115-1300 v171 aLyons, Berry1 aWelch, Kathleen, A.1 aFountain, Andrew, G1 aDana, Gayle, L.1 aVaughn, Bruce, H.1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/surface-glaciochemistry-taylor-valley-southern-victoria-land-antarctica-and-its-relationship00890nas a2200277 4500008004100000245006500041210006500106260001200171300001200183490000800195653002100203100002100224700002100245700001700266700002000283700002400303700002400327700002400351700002300375700002000398700001900418700002700437700002700464700002400491856009700515 2002 eng d00aAntarctic climate cooling and terrestrial ecosystem response0 aAntarctic climate cooling and terrestrial ecosystem response c01/2002 a517-5200 v41510aClimate Response1 aDoran, Peter, T.1 aPriscu, John, C.1 aLyons, Berry1 aWalsh, John, E.1 aFountain, Andrew, G1 aMcKnight, Diane, M.1 aMoorhead, Daryl, L.1 aVirginia, Ross, A.1 aWall, Diana, H.1 aClow, Gary, D.1 aFritsen, Christian, H.1 aMcKay, Christopher, P.1 aParsons, Andrew, N. uhttps://mcm.lternet.edu/content/antarctic-climate-cooling-and-terrestrial-ecosystem-response00529nas a2200121 4500008004100000245010400041210006900145300001400214490000700228100001600235700002800251856012800279 2002 eng d00aThe balance between photosynthesis and grazing in Antarctic mixotrophic cryptophytes during summer0 abalance between photosynthesis and grazing in Antarctic mixotrop a2060-20700 v471 aMarshall, W1 aLaybourn-Parry, Johanna uhttps://mcm.lternet.edu/content/balance-between-photosynthesis-and-grazing-antarctic-mixotrophic-cryptophytes-during-summer00517nas a2200121 4500008004100000245009300041210006900134260002700203490000900230100001700239700002400256856011500280 2002 eng d00aClimatic controls on interannual variation in streamflow in Fryxell Basin, Taylor Valley0 aClimatic controls on interannual variation in streamflow in Fryx bUniversity of Colorado0 vM.S.1 aJaros, Chris1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/climatic-controls-interannual-variation-streamflow-fryxell-basin-taylor-valley00614nas a2200157 4500008004100000245010900041210006900150300001400219490000700233100001500240700002400255700001200279700001500291700002500306856012500331 2002 eng d00aDirect observation of aluminosilicate weathering in the hyporheic zone of an Antarctic Dry Valley stream0 aDirect observation of aluminosilicate weathering in the hyporhei a1335-13470 v661 aMaurice, P1 aMcKnight, Diane, M.1 aLeff, L1 aFulghun, J1 aGooseff, Michael, N. uhttps://mcm.lternet.edu/content/direct-observation-aluminosilicate-weathering-hyporheic-zone-antarctic-dry-valley-stream00625nas a2200145 4500008004100000245013200041210006900173300001200242490000700254100002400261700002000285700002300305700002400328856012700352 2002 eng d00aDistribution and life cycle of Scottnema lindsayae (Nematoda) in Antarctic soils: A modeling analysis of tempaerature responses0 aDistribution and life cycle of Scottnema lindsayae Nematoda in A a118-1250 v251 aMoorhead, Daryl, L.1 aWall, Diana, H.1 aVirginia, Ross, A.1 aParsons, Andrew, N. uhttps://mcm.lternet.edu/content/distribution-and-life-cycle-scottnema-lindsayae-nematoda-antarctic-soils-modeling-analysis00470nas a2200121 4500008004100000245006800041210006800109260002700177490000900204100001700213700002400230856009400254 2002 eng d00aInterannual variation in phytoplankton dynamics in Lake Fryxell0 aInterannual variation in phytoplankton dynamics in Lake Fryxell bUniversity of Colorado0 vM.S.1 aVan Matre, E1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/interannual-variation-phytoplankton-dynamics-lake-fryxell00772nas a2200253 4500008004100000245004700041210004700088300001200135490000800147100002000155700001900175700002700194700002700221700002400248700002100272700002100293700001700314700002400331700002400355700002400379700002300403700002000426856007200446 2002 eng d00aRecent Temperature Trends in the Antarctic0 aRecent Temperature Trends in the Antarctic a291-2920 v4181 aWalsh, John, E.1 aClow, Gary, D.1 aFritsen, Christian, H.1 aMcKay, Christopher, P.1 aParsons, Andrew, N.1 aDoran, Peter, T.1 aPriscu, John, C.1 aLyons, Berry1 aFountain, Andrew, G1 aMcKnight, Diane, M.1 aMoorhead, Daryl, L.1 aVirginia, Ross, A.1 aWall, Diana, H. uhttps://mcm.lternet.edu/content/recent-temperature-trends-antarctic03551nas a2200217 4500008004100000245009000041210006900131260001200200300001400212490000800226520282700234653001103061100002103072700002703093700001903120700002003139700002403159700002203183700001703205856011103222 2002 eng d00aValley floor climate observations from the McMurdo Dry Valleys, Antarctica, 1986-20000 aValley floor climate observations from the McMurdo Dry Valleys A c12/2002 a4772-47840 v1073 a

Climate observations from the McMurdo dry valleys, East Antarctica are presented from a network of seven valley floor automatic meteorological stations during the period 1986 to 2000. Mean annual temperatures ranged from −14.8°C to −30.0°C, depending on the site and period of measurement. Mean annual relative humidity is generally highest near the coast. Mean annual wind speed increases with proximity to the polar plateau. Site-to-site variation in mean annual solar flux and PAR is due to exposure of each station and changes over time are likely related to changes in cloudiness. During the nonsummer months, strong katabatic winds are frequent at some sites and infrequent at others, creating large variation in mean annual temperature owing to the warming effect of the winds. Katabatic wind exposure appears to be controlled to a large degree by the presence of colder air in the region that collects at low points and keeps the warm less dense katabatic flow from the ground. The strong influence of katabatic winds makes prediction of relative mean annual temperature based on geographical position (elevation and distance from the coast) alone, not possible. During the summer months, onshore winds dominate and warm as they progress through the valleys creating a strong linear relationship (r2 = 0.992) of increasing potential temperature with distance from the coast (0.09°C km−1). In contrast to mean annual temperature, summer temperature lends itself quite well to model predictions, and is used to construct a statistical model for predicting summer dry valley temperatures at unmonitored sites.

10aBiggie1 aDoran, Peter, T.1 aMcKay, Christopher, P.1 aClow, Gary, D.1 aDana, Gayle, L.1 aFountain, Andrew, G1 aNylen, Thomas, H.1 aLyons, Berry uhttps://mcm.lternet.edu/content/valley-floor-climate-observations-mcmurdo-dry-valleys-antarctica-1986-200000603nas a2200169 4500008004100000245014000041210006900181260001200250300001400262490000700276653001100283100002500294700002400319700001700343700001200360856006100372 2002 eng d00aWeathering reactions and hyporheic exchange controls on stream water chemistry in a glacial meltwater stream in the McMurdo Dry Valleys0 aWeathering reactions and hyporheic exchange controls on stream w c12/2002 a1279-12960 v3810aBiggie1 aGooseff, Michael, N.1 aMcKnight, Diane, M.1 aLyons, Berry1 aBlum, A uhttp://www.agu.org/pubs/crossref/2002/2001WR000834.shtml00506nas a2200157 4500008004100000245007600041210006900117260001200186300001400198490000700212653001100219100003300230700002100263700002400284856004000308 2001 eng d00aBacterial dissolved organic carbon demand in antarctic dry valley lakes0 aBacterial dissolved organic carbon demand in antarctic dry valle c07/2001 a1189-11940 v4610aBiggie1 aTakacs-Vesbach, Cristina, D.1 aPriscu, John, C.1 aMcKnight, Diane, M. uhttp://www.jstor.org/stable/267103100696nas a2200157 4500008004100000245019500041210006900236300001000305490000700315100001500322700002400337700001800361700001700379700001600396856012600412 2001 eng d00aBenthic primary production in two perennially ice-covered Antarctic lakes: comparisons of annual accumulation predicted from photosynthesis models with estimates from internal growth markers0 aBenthic primary production in two perennially icecovered Antarct a18-270 v131 aHawes, Ian1 aMoorhead, Daryl, L.1 aSutherland, J1 aSchmeling, J1 aSchwartz, A uhttps://mcm.lternet.edu/content/benthic-primary-production-two-perennially-ice-covered-antarctic-lakes-comparisons-annual00572nas a2200145 4500008004100000245009000041210006900131300001200200490000800212100002300220700002500243700001500268700002700283856011600310 2001 eng d00aGlacial cryoconite ecosystems: a bipolar comparison of algal communities and habitats0 aGlacial cryoconite ecosystems a bipolar comparison of algal comm a173-1970 v1231 aMueller, Derek, R.1 aVincent, Warwick, F.1 aPollard, W1 aFritsen, Christian, H. uhttps://mcm.lternet.edu/content/glacial-cryoconite-ecosystems-bipolar-comparison-algal-communities-and-habitats00534nas a2200121 4500008004100000245010800041210006900149260002700218490000900245100001300254700002400267856012100291 2001 eng d00aThe influence of mixotroph growth on DOM chemistry in Pony Lake, a eutrophic coastal pond in Antarctica0 ainfluence of mixotroph growth on DOM chemistry in Pony Lake a eu bUniversity of Colorado0 vM.S.1 aBrown, A1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/influence-mixotroph-growth-dom-chemistry-pony-lake-eutrophic-coastal-pond-antarctica00756nas a2200193 4500008004100000245013700041210006900178490000600247100002600253700002100279700002900300700001700329700002300346700001300369700001600382700001500398700002000413856012900433 2001 eng d00aThe influence of soil biodiversity on hydrological pathways and the transfer of materials between terrestrial and aquatic ecosystems0 ainfluence of soil biodiversity on hydrological pathways and the 0 v41 aBardgett, Richard, D.1 aAnderson, J., M.1 aBehan-Pelletier, Valerie1 aBrussaard, L1 aColeman, David, C.1 aEttma, C1 aMoldenke, A1 aSchimel, J1 aWall, Diana, H. uhttps://mcm.lternet.edu/content/influence-soil-biodiversity-hydrological-pathways-and-transfer-materials-between-terrestrial00774nas a2200193 4500008004100000245014400041210006900185300001200254490000700266100001700273700002400290700002100314700002800335700002400363700002400387700002100411700002000432856012800452 2001 eng d00aThe McMurdo Dry Valleys Long-Term Ecological Research Program: new understanding of the biogeochemistry of the Dry Valley lakes: a review.0 aMcMurdo Dry Valleys LongTerm Ecological Research Program new und a202-2170 v251 aLyons, Berry1 aWelch, Kathleen, A.1 aPriscu, John, C.1 aLaybourn-Parry, Johanna1 aMoorhead, Daryl, L.1 aMcKnight, Diane, M.1 aDoran, Peter, T.1 aTranter, Martyn uhttps://mcm.lternet.edu/content/mcmurdo-dry-valleys-long-term-ecological-research-program-new-understanding-biogeochemistry03621nas a2200133 4500008004100000245010500041210006900146260002700215490001000242520306700252100002503319700002403344856011903368 2001 eng d00aModeling hyporheic exchange influences on biogeochemical processes in dry valley streams, Antarctica0 aModeling hyporheic exchange influences on biogeochemical process bUniversity of Colorado0 vPh.D.3 a

The ephemeral streams of the Dry Valleys of Antarctica provide habitat to benthic algal mats, and greatly control the quantity and quality of glacial melt water that enters closed basin Dry Valley lakes. Dry Valley watersheds are composed of streambeds and adjacent hyporheic zones. Hydrologic exchange of water and solutes between the stream and the hyporheic zone has the overall effect of increasing residence time in the stream/hyporheic system. Biogeochemical reactions (e.g. chemical weathering, nutrient assimilation) occur both in the water column and in the hyporheic zone. Field experiments and solute transport modeling were employed to elucidate the effects of rapid hyporheic exchange on biogeochemical cycling in Antarctic streams. The results presented here show that (1) large portions of the wetted zone that surrounds each stream is a hyporheic zone, and that stream water exchanges into and out of extended portions of this zone on the order of weeks, (2) the rapid exchange of stream water between the water column and the hyporheic zone controls the rate of weathering in streambed sediments, and (3) denitrification in streams is limited by the conversion of NO2 to N2O, while the conversion of NO3 to NO2 occurs very quickly.

1 aGooseff, Michael, N.1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/modeling-hyporheic-exchange-influences-biogeochemical-processes-dry-valley-streams00492nas a2200121 4500008004100000245008000041210006900121260002700190490000900217100002100226700002400247856009900271 2001 eng d00aRegrowth of cyanobacterial mats in Greek Creek and the impact of ecotourism0 aRegrowth of cyanobacterial mats in Greek Creek and the impact of bUniversity of Colorado0 vM.S.1 aChatfield, Ethan1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/regrowth-cyanobacterial-mats-greek-creek-and-impact-ecotourism00661nas a2200145 4500008004100000245017000041210006900211300001200280490000700292653002100299100001900320700002400339700002400363856012800387 2001 eng d00aRetrospective simulation of lake level rise in Lake Bonney based on recent 21-year record: indication of recent climate change in the McMurdo Dry Valleys, Antarctica0 aRetrospective simulation of lake level rise in Lake Bonney based a477-4920 v2510aClimate Response1 aBomblies, Arne1 aMcKnight, Diane, M.1 aAndrews, Edmund, D. uhttps://mcm.lternet.edu/content/retrospective-simulation-lake-level-rise-lake-bonney-based-recent-21-year-record-indication00551nas a2200121 4500008004100000245011800041210006900159260002500228490000900253100001700262700002400279856012600303 2001 eng d00aSensitivity of carbon dynamics to changing light availability of benthic microbial mats in Lake Hoare, Antarctica0 aSensitivity of carbon dynamics to changing light availability of bUniversity of Toledo0 vM.S.1 aSchmeling, J1 aMoorhead, Daryl, L. uhttps://mcm.lternet.edu/content/sensitivity-carbon-dynamics-changing-light-availability-benthic-microbial-mats-lake-hoare00706nas a2200169 4500008004100000245014800041210006900189300001000258490000700268100002400275700002500299700002500324700002100349700001800370700002300388856012500411 2001 eng d00aSpectrofluorometric characterization of aquatic fulvic acid for determination of precursor organic material and general structural properties.0 aSpectrofluorometric characterization of aquatic fulvic acid for  a38-480 v461 aMcKnight, Diane, M.1 aBoyer, Elizabeth, W.1 aWesterhoff, Paul, K.1 aDoran, Peter, T.1 aKulbe, Thomas1 aAndersen, Dale, T. uhttps://mcm.lternet.edu/content/spectrofluorometric-characterization-aquatic-fulvic-acid-determination-precursor-organic00553nas a2200121 4500008004100000245010800041210006900149260002700218490000900245100002400254700002400278856012900302 2000 eng d00aActive layer dynamics and hyporheic zone storage in three streams in the McMurdo Dy Valleys, Antarctica0 aActive layer dynamics and hyporheic zone storage in three stream bUniversity of Colorado0 vM.S.1 aConovitz, Peter, A.1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/active-layer-dynamics-and-hyporheic-zone-storage-three-streams-mcmurdo-dy-valleys-antarctica00868nas a2200229 4500008004100000245013400041210006900175260002800244300001200272100002400284700001700308700002400325700001700349700002400366700002200390700002100412700002700433700001800460700001900478700001800497856012300515 2000 eng d00aClimate and hydrologic variations and implications for lake and stream ecological response in the McMurdo Dry Valleys, Antarctica0 aClimate and hydrologic variations and implications for lake and  bOxford University Press a174-1951 aWelch, Kathleen, A.1 aLyons, Berry1 aMcKnight, Diane, M.1 aJaros, Chris1 aFountain, Andrew, G1 aNylen, Thomas, H.1 aDoran, Peter, T.1 aHoward-Williams, Clive1 aGreenland, D.1 aGoodin, D., G.1 aSmith, R., C. uhttps://mcm.lternet.edu/content/climate-and-hydrologic-variations-and-implications-lake-and-stream-ecological-response00520nas a2200121 4500008004100000245010100041210006900142260002500211490000900236100001500245700002400260856011400284 2000 eng d00aDynamics of the deep chlorophyll maximum within the lakes of the McMurdo Dry Valleys, Antarctica0 aDynamics of the deep chlorophyll maximum within the lakes of the bUniversity of Toledo0 vM.S.1 aBurnett, L1 aMoorhead, Daryl, L. uhttps://mcm.lternet.edu/content/dynamics-deep-chlorophyll-maximum-within-lakes-mcmurdo-dry-valleys-antarctica00602nas a2200169 4500008004100000245007200041210006900113260003000182300001200212100002400224700001600248700002200264700001500286700001400301700001600315856010100331 2000 eng d00aExperimental Approaches to Investigate Belowground Animal Diversity0 aExperimental Approaches to Investigate Belowground Animal Divers aNew YorkbSpringer Verlag a318-3291 aFreckman, Diana, W.1 aReichman, O1 aSala, Osvaldo, E.1 aJackson, R1 aMooney, H1 aHowarth, R. uhttps://mcm.lternet.edu/content/experimental-approaches-investigate-belowground-animal-diversity00870nas a2200325 4500008004100000245005200041210005200093300001400145490000800159100002200167700001400189700001500203700001300218700001400231700001800245700001200263700001300275700001400288700001500302700002000317700001900337700001500356700001400371700001800385700001300403700002100416700001600437700001500453856007600468 2000 eng d00aGlobal biodiversity scenarios for the year 21000 aGlobal biodiversity scenarios for the year 2100 a1770-17740 v2871 aSala, Osvaldo, E.1 aKinzig, A1 aLeemans, R1 aLodge, D1 aMooney, H1 aOesterheld, M1 aPoff, N1 aSykes, M1 aWalker, B1 aWalkder, M1 aWall, Diana, H.1 aChapin, F., S.1 aArmesto, J1 aBerlow, E1 aBloomfield, J1 aDirzo, R1 aHuber-Sanwald, E1 aHuenneke, L1 aJackson, R uhttps://mcm.lternet.edu/content/global-biodiversity-scenarios-year-210000632nas a2200181 4500008004100000245008800041210006900129260001200198300001200210490000700222653001100229653001100240100002400251700001800275700001900293700002300312856011500335 2000 eng d00aPhytoplankton dynamics in a stably stratified Antarctic lake during winter darkness0 aPhytoplankton dynamics in a stably stratified Antarctic lake dur c10/2000 a852-8610 v3610aBiggie10awinter1 aMcKnight, Diane, M.1 aHowes, B., L.1 aTaylor, C., D.1 aGoehringer, D., D. uhttps://mcm.lternet.edu/content/phytoplankton-dynamics-stably-stratified-antarctic-lake-during-winter-darkness00609nas a2200181 4500008004100000245009000041210006900131260001200200300001200212490000700224653001100231653001300242100002300255700002800278700002400306700001600330856008100346 2000 eng d00aStratification and dynamics of microbial loop communities in Lake Fryxell, Antarctica0 aStratification and dynamics of microbial loop communities in Lak c12/2001 a649-6610 v4410aBiggie10aprotozoa1 aRoberts, Emily, C.1 aLaybourn-Parry, Johanna1 aMcKnight, Diane, M.1 aNovarino, G uhttp://onlinelibrary.wiley.com/doi/10.1046/j.1365-2427.2000.00612.x/abstract00587nas a2200169 4500008004100000245006800041210006600109300001200175490000700187100002400194700002000218700002500238700001900263700002400282700002000306856009100326 1999 eng d00aDry Valley streams in Antarctica: ecosystems waiting for water0 aDry Valley streams in Antarctica ecosystems waiting for water a985-9950 v491 aMcKnight, Diane, M.1 aNiyogi, Dev, K.1 aAlger, Alexander, S.1 aBomblies, Arne1 aConovitz, Peter, A.1 aTate, Cathy, M. uhttps://mcm.lternet.edu/content/dry-valley-streams-antarctica-ecosystems-waiting-water00781nas a2200241 4500008004100000245006000041210005900101300001400160490000800174100002100182700002200203700001700225700002100242700002000263700002200283700002700305700003300332700002400365700002000389700002500409700001600434856008900450 1999 eng d00aGeomicrobiology of sub-glacial ice above Vostok Station0 aGeomicrobiology of subglacial ice above Vostok Station a2141-21440 v2861 aPriscu, John, C.1 aAdams, Edward, E.1 aLyons, Berry1 aVoytek, Mary, A.1 aMogk, David, W.1 aBrown, Robert, L.1 aMcKay, Christopher, P.1 aTakacs-Vesbach, Cristina, D.1 aWelch, Kathleen, A.1 aWolf, Craig, F.1 aKirshtein, Julie, D.1 aAvci, Recep uhttps://mcm.lternet.edu/content/geomicrobiology-sub-glacial-ice-above-vostok-station00485nas a2200121 4500008004100000245008200041210006900123300001200192490000700204100002000211700002000231856011200251 1999 eng d00aInteractions underground: soil biodiversity, mutalism and ecosystem processes0 aInteractions underground soil biodiversity mutalism and ecosyste a109-1190 v491 aWall, Diana, H.1 aMoore, John, C. uhttps://mcm.lternet.edu/content/interactions-underground-soil-biodiversity-mutalism-and-ecosystem-processes00878nas a2200277 4500008004100000245006500041210006400106260001200170300001200182490000700194653002300201100002400224700001700248700002400265700002000289700002100309700002100330700002400351700002400375700002400399700002100423700002000444700002400464700002300488856008900511 1999 eng d00aPhysical controls on the Taylor Valley Ecosystem, Antarctica0 aPhysical controls on the Taylor Valley Ecosystem Antarctica c12/1999 a961-9720 v4910aWater availability1 aFountain, Andrew, G1 aLyons, Berry1 aBurkins, Melody, B.1 aDana, Gayle, L.1 aDoran, Peter, T.1 aLewis, Karen, J.1 aMcKnight, Diane, M.1 aMoorhead, Daryl, L.1 aParsons, Andrew, N.1 aPriscu, John, C.1 aWall, Diana, H.1 aWharton, Robert, A.1 aVirginia, Ross, A. uhttps://mcm.lternet.edu/content/physical-controls-taylor-valley-ecosystem-antarctica00502nas a2200145 4500008004100000245010400041210006900145260001200214300001200226490000700238100002300245700002400268700002400292856004000316 1998 eng d00aAnalysis of transient storage subject to unsteady flow: diel flow variation in an Antarctic stream0 aAnalysis of transient storage subject to unsteady flow diel flow c06/1998 a143-1540 v171 aRunkel, Robert, L.1 aMcKnight, Diane, M.1 aAndrews, Edmund, D. uhttp://www.jstor.org/stable/146795800570nas a2200157 4500008004100000245007400041210006900115260001200184300001600196490000800212100002100220700002400241700002500265700002700290856009500317 1998 eng d00aAntarctic paleolake sediments and the search for extinct life on Mars0 aAntarctic paleolake sediments and the search for extinct life on c05/1998 a28481-284930 v1031 aDoran, Peter, T.1 aWharton, Robert, A.1 aDesMarais, David, J.1 aMcKay, Christopher, P. uhttps://mcm.lternet.edu/content/antarctic-paleolake-sediments-and-search-extinct-life-mars00499nas a2200121 4500008004100000245007100041210006900112260004900181490000900230100001900239700002400258856009500282 1998 eng d00aClimatic controls on streamflow generation from Antarctic glaciers0 aClimatic controls on streamflow generation from Antarctic glacie aBoulder, CObUniversity of Colorado Boulder0 vM.S.1 aBomblies, Arne1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/climatic-controls-streamflow-generation-antarctic-glaciers00542nas a2200133 4500008004100000245009200041210006900133300001200202490000700214100002300221700002700244700002400271856011300295 1998 eng d00aDissolved gases in perennially ice-covered lakes of the McMurdo Dry Valleys, Antarctica0 aDissolved gases in perennially icecovered lakes of the McMurdo D a124-1330 v101 aAndersen, Dale, T.1 aMcKay, Christopher, P.1 aWharton, Robert, A. uhttps://mcm.lternet.edu/content/dissolved-gases-perennially-ice-covered-lakes-mcmurdo-dry-valleys-antarctica00747nas a2200205 4500008004100000245009700041210006900138300001000207490000700217100001700224700002400241700001900265700002300284700002000307700002300327700002400350700002400374700002100398856012200419 1998 eng d00aGeochemical Linkages Among Glaciers, Streams, and Lakes Within the Taylor Valley, Antarctica0 aGeochemical Linkages Among Glaciers Streams and Lakes Within the a77-920 v721 aLyons, Berry1 aWelch, Kathleen, A.1 aNeumann, Klaus1 aToxey, Jeffrey, K.1 aMcArthur, Robyn1 aWilliams, Changela1 aMcKnight, Diane, M.1 aMoorhead, Daryl, L.1 aPriscu, John, C. uhttps://mcm.lternet.edu/content/geochemical-linkages-among-glaciers-streams-and-lakes-within-taylor-valley-antarctica00571nas a2200157 4500008004100000245007600041210006900117300001000186490000700196100002400203700002000227700002100247700002200268700002400290856009900314 1998 eng d00aGlaciers of the McMurdo Dry Valleys, Southern Victoria Land, Antarctica0 aGlaciers of the McMurdo Dry Valleys Southern Victoria Land Antar a65-750 v721 aFountain, Andrew, G1 aDana, Gayle, L.1 aLewis, Karen, J.1 aVaughn, Bruce, H.1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/glaciers-mcmurdo-dry-valleys-southern-victoria-land-antarctica00605nas a2200157 4500008004100000245008700041210006900128300001100197490000700208100002400215700002400239700002300263700002400286700002200310856011500332 1998 eng d00aHydrologic Processes Influencing Streamflow Variation in Fryxell Basin, Antarctica0 aHydrologic Processes Influencing Streamflow Variation in Fryxell a93-1080 v721 aConovitz, Peter, A.1 aMcKnight, Diane, M.1 aMacDonald, Lee, H.1 aFountain, Andrew, G1 aHouse, Harold, R. uhttps://mcm.lternet.edu/content/hydrologic-processes-influencing-streamflow-variation-fryxell-basin-antarctica00570nas a2200133 4500008004100000245012200041210006900163300001200232490000700244100001900251700001700270700002200287856012700309 1998 eng d00aInorganic carbon-isotope distribution and budget in the Lake Hoare and Lake Fryxell basins, Taylor Valley, Antarctica0 aInorganic carbonisotope distribution and budget in the Lake Hoar a685-6900 v271 aNeumann, Klaus1 aLyons, Berry1 aMarais, D.J., Des uhttps://mcm.lternet.edu/content/inorganic-carbon-isotope-distribution-and-budget-lake-hoare-and-lake-fryxell-basins-taylor00612nas a2200157 4500008004100000245009600041210006900137300001200206490000700218100001700225700002100242700002400263700002400287700002200311856012100333 1998 eng d00aA late holocene dessication of Lake Hoare and Lake Fryxell, McMurdo Dry Valleys, Antarctica0 alate holocene dessication of Lake Hoare and Lake Fryxell McMurdo a247-2560 v101 aLyons, Berry1 aTyler, Scott, W.1 aWharton, Robert, A.1 aMcKnight, Diane, M.1 aVaughn, Bruce, H. uhttps://mcm.lternet.edu/content/late-holocene-dessication-lake-hoare-and-lake-fryxell-mcmurdo-dry-valleys-antarctica00749nas a2200157 4500008004100000245022300041210006900264300001200333490000700345100002400352700002500376700002000401700002100421700002500442856012400467 1998 eng d00aLongitudinal Patterns in Algal Abundance and Species Distribution in Meltwater Streams in Taylor Valley, Southern Victoria Land, Antarctica, in Ecosystem Processes in a Polar Desert: The McMurdo Dry Valleys, Antarctica0 aLongitudinal Patterns in Algal Abundance and Species Distributio a109-1270 v721 aMcKnight, Diane, M.1 aAlger, Alexander, S.1 aTate, Cathy, M.1 aShupe, Gordon, H1 aSpaulding, Sarah, A. uhttps://mcm.lternet.edu/content/longitudinal-patterns-algal-abundance-and-species-distribution-meltwater-streams-taylor00576nas a2200121 4500008004100000245015400041210006900195300001200264490000700276100002400283700002100307856012600328 1998 eng d00aThe McMurdo Dry Valley Ecosystem: Organization, Controls, and Linkages, in Ecosystem Processes in a Polar Desert: The McMurdo Dry Valleys, Antarctica0 aMcMurdo Dry Valley Ecosystem Organization Controls and Linkages  a351-3630 v721 aMoorhead, Daryl, L.1 aPriscu, John, C. uhttps://mcm.lternet.edu/content/mcmurdo-dry-valley-ecosystem-organization-controls-and-linkages-ecosystem-processes-polar00566nas a2200121 4500008004100000245014700041210006900188300001200257490000700269100002400276700002400300856012000324 1998 eng d00aMcMurdo Dry Valleys LTER: Stream discharge as a function of ambient temperature and incoming shortwave radiation in Taylor Valley, Antarctica0 aMcMurdo Dry Valleys LTER Stream discharge as a function of ambie a196-1970 v311 aMoorhead, Daryl, L.1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/mcmurdo-dry-valleys-lter-stream-discharge-function-ambient-temperature-and-incoming00602nas a2200145 4500008004100000245012200041210006900163300001200232490000800244100001700252700001600269700002400285700001900309856012800328 1998 eng d00aMercury speciation and distribution in a polar desert lake (Lake Hoare, Antarctica) and two glacial meltwater streams0 aMercury speciation and distribution in a polar desert lake Lake  a229-2370 v2131 aVandal, G.M.1 aMason, R.P.1 aMcKnight, Diane, M.1 aFitzgerald, W. uhttps://mcm.lternet.edu/content/mercury-speciation-and-distribution-polar-desert-lake-lake-hoare-antarctica-and-two-glacial00608nas a2200133 4500008004100000245015100041210006900192300001200261490000700273100002400280700002400304700002000328856012600348 1998 eng d00aModeling Nitrogen Transformations in Dry Valley Streams, Antarctica, in Ecosystem Processes in a Polar Desert: The McMurdo Dry Valleys, Antarctica0 aModeling Nitrogen Transformations in Dry Valley Streams Antarcti a141-1510 v721 aMoorhead, Daryl, L.1 aMcKnight, Diane, M.1 aTate, Cathy, M. uhttps://mcm.lternet.edu/content/modeling-nitrogen-transformations-dry-valley-streams-antarctica-ecosystem-processes-polar00762nas a2200241 4500008004100000245007100041210006900112260001200181300001400193490000800207653001100215100002100226700002700247700002200274700002800296700002000324700002700344700002100371700002400392700002200416700002400438856005800462 1998 eng d00aPerennial Antarctic Lake Ice: An Oasis for Life in a Polar Desert0 aPerennial Antarctic Lake Ice An Oasis for Life in a Polar Desert c06/1998 a2095-20980 v28010aBiggie1 aPriscu, John, C.1 aFritsen, Christian, H.1 aAdams, Edward, E.1 aGiovannoni, Stephen, J.1 aPaerl, Hans, W.1 aMcKay, Christopher, P.1 aDoran, Peter, T.1 aGordon, Douglas, A.1 aLanoil, Brian, D.1 aPinckney, James, L. uhttp://www.sciencemag.org/content/280/5372/2095.short00581nas a2200145 4500008004100000245009300041210006900134300001200203490000700215100002700222700002200249700002700271700002100298856011600319 1998 eng d00aPermanent Ice Covers of the McMurdo Dry Valleys Lakes, Antarctica: Liquid Water Contents0 aPermanent Ice Covers of the McMurdo Dry Valleys Lakes Antarctica a269-2800 v721 aFritsen, Christian, H.1 aAdams, Edward, E.1 aMcKay, Christopher, P.1 aPriscu, John, C. uhttps://mcm.lternet.edu/content/permanent-ice-covers-mcmurdo-dry-valleys-lakes-antarctica-liquid-water-contents00569nas a2200121 4500008004100000245013800041210006900179260002600248490000900274100001300283700002400296856012700320 1998 eng d00aSpatial-temporal patterns of primary production in phytoplankton communities of Taylor Valley lakes, Antartica: A modelling synthesis0 aSpatialtemporal patterns of primary production in phytoplankton  bTexas Tech University0 vM.S.1 aDavis, W1 aMoorhead, Daryl, L. uhttps://mcm.lternet.edu/content/spatial-temporal-patterns-primary-production-phytoplankton-communities-taylor-valley-lakes00640nas a2200157 4500008004100000245011400041210006900155300001200224490000700236100002400243700002600267700002100293700002300314700002400337856012100361 1998 eng d00aStructure and composition of the photochemical apparatus of the Antarctic green alga Chlamydomonas subcaudata0 aStructure and composition of the photochemical apparatus of the  a303-3140 v561 aMorgan, Rachael, M.1 aIvanov, Alexander, G.1 aPriscu, John, C.1 aMaxwell, Denis, P.1 aHuner, Norman, P.A. uhttps://mcm.lternet.edu/content/structure-and-composition-photochemical-apparatus-antarctic-green-alga-chlamydomonas00510nas a2200121 4500008004100000245009700041210006900138300001000207490000700217100002400224700002000248856012000268 1997 eng d00aCanada Stream: a glacial meltwater stream in Taylor Valley, South Victoria Land, Antarctica0 aCanada Stream a glacial meltwater stream in Taylor Valley South  a14-170 v161 aMcKnight, Diane, M.1 aTate, Cathy, M. uhttps://mcm.lternet.edu/content/canada-stream-glacial-meltwater-stream-taylor-valley-south-victoria-land-antarctica00661nas a2200169 4500008004100000245009900041210006900140260002900209300001200238100002400250700001700274700002400291700001700315700002700332700001500359856011700374 1997 eng d00aCarbon dynamics of aquatic microbial mats in the Antarctic dry valleys: A modelling synthesis0 aCarbon dynamics of aquatic microbial mats in the Antarctic dry v bBalkema Press, Rotterdam a181-1961 aMoorhead, Daryl, L.1 aDavis, Shane1 aWharton, Robert, A.1 aLyons, Berry1 aHoward-Williams, Clive1 aHawes, Ian uhttps://mcm.lternet.edu/content/carbon-dynamics-aquatic-microbial-mats-antarctic-dry-valleys-modelling-synthesis00811nas a2200217 4500008004100000245012400041210006900165260002900234300001200263100001700275700002400292700001800316700001400334700002300348700001900371700002400390700001700414700002700431700001500458856012000473 1997 eng d00aChemical weathering rates and reactions in the Lake Fryxell Basin, Taylor Valley : Comparison to temperate river basins0 aChemical weathering rates and reactions in the Lake Fryxell Basi bBalkema Press, Rotterdam a147-1541 aLyons, Berry1 aWelch, Kathleen, A.1 aNezat, C., A.1 aCrick, K.1 aToxey, Jeffrey, K.1 aMastrine, J.A.1 aMcKnight, Diane, M.1 aLyons, Berry1 aHoward-Williams, Clive1 aHawes, Ian uhttps://mcm.lternet.edu/content/chemical-weathering-rates-and-reactions-lake-fryxell-basin-taylor-valley-comparison00670nas a2200181 4500008004100000245009200041210006900133260002900202300001200231100001700243700002200260700002300282700002100305700001700326700002700343700001500370856010300385 1997 eng d00aClimate history of the McMurdo Dry Valleys since the last glacial maximum: A synthesis0 aClimate history of the McMurdo Dry Valleys since the last glacia bBalkema Press, Rotterdam a155-1621 aLyons, Berry1 aBartek, Louis, R.1 aMayewski, Paul, A.1 aDoran, Peter, T.1 aLyons, Berry1 aHoward-Williams, Clive1 aHawes, Ian uhttps://mcm.lternet.edu/content/climate-history-mcmurdo-dry-valleys-last-glacial-maximum-synthesis00640nas a2200145 4500008004100000245015200041210006900193300001200262490000700274100002500281700002400306700001900330700002100349856012400370 1997 eng d00aDiatoms in sediments of perennially ice-covered Lake Hoare, and implications for interpreting lake history in the McMurdo Dry Valleys of Antarctica0 aDiatoms in sediments of perennially icecovered Lake Hoare and im a403-4200 v171 aSpaulding, Sarah, A.1 aMcKnight, Diane, M.1 aStoermer, E.F.1 aDoran, Peter, T. uhttps://mcm.lternet.edu/content/diatoms-sediments-perennially-ice-covered-lake-hoare-and-implications-interpreting-lake00801nas a2200217 4500008004100000245015200041210006900193260002700262300001100289490000700300100002500307700002400332700002500356700002000381700002100401700002400422700002000446700002400466700001600490856007700506 1997 eng d00aEcological processes in a cold desert ecosystem: the abundance and species distribution of algal mats in glacial meltwater streams in Taylor Valley0 aEcological processes in a cold desert ecosystem the abundance an bUniversity of Colorado a108 pp0 v511 aAlger, Alexander, S.1 aMcKnight, Diane, M.1 aSpaulding, Sarah, A.1 aTate, Cathy, M.1 aShupe, Gordon, H1 aWelch, Kathleen, A.1 aEdwards, R., L.1 aAndrews, Edmund, D.1 aHouse, H.R. uhttp://instaar.colorado.edu/other/download/OP51-ECOLOGICAL-PROCESSES.pdf00578nas a2200133 4500008004100000245011900041210006900160300001400229490000700243100002400250700002000274700002400294856012600318 1997 eng d00aImpact of Light Regimes on Productivity Patterns of Benthic Microbial Mats in an Antarctic Lake: A Modeling Study0 aImpact of Light Regimes on Productivity Patterns of Benthic Micr a1561-15690 v421 aMoorhead, Daryl, L.1 aWolf, Craig, F.1 aWharton, Robert, A. uhttps://mcm.lternet.edu/content/impact-light-regimes-productivity-patterns-benthic-microbial-mats-antarctic-lake-modeling00675nas a2200169 4500008004100000245011500041210006900156300001000225490000700235100002800242700002000270700002400290700002100314700002500335700001900360856012600379 1997 eng d00aThe microbial plankton of Lake Fryxell, southern Victoria Land, Antarctica during the summers of 1992 and 19940 amicrobial plankton of Lake Fryxell southern Victoria Land Antarc a54-610 v171 aLaybourn-Parry, Johanna1 aJames, Mark, R.1 aMcKnight, Diane, M.1 aPriscu, John, C.1 aSpaulding, Sarah, A.1 aShiel, Russell uhttps://mcm.lternet.edu/content/microbial-plankton-lake-fryxell-southern-victoria-land-antarctica-during-summers-1992-and00806nas a2200253 4500008004100000245008400041210006900125260001200194300001000206490000600216100001900222700001800241700002400259700001800283700001400301700001600315700001700331700001400348700001800362700002400380700001600404700002300420856010900443 1997 eng d00aOpen-top Designs for Manipulating Field Temperature in High-Latitude Ecosystems0 aOpentop Designs for Manipulating Field Temperature in HighLatitu c12/1997 a20-320 v31 aMarion, G., M.1 aHenry, G.H.R.1 aFreckman, Diana, W.1 aJohnstone, J.1 aJones, G.1 aJones, M.H.1 aLevesque, E.1 aMolau, U.1 aMØLGAARD, P.1 aParsons, Andrew, N.1 aSvoboda, J.1 aVirginia, Ross, A. uhttps://mcm.lternet.edu/content/open-top-designs-manipulating-field-temperature-high-latitude-ecosystems00795nas a2200193 4500008004100000245015600041210006900197260002900266300001200295100002000307700002000327700002400347700001900371700002500390700001700415700002700432700001500459856012700474 1997 eng d00aSpecies composition and primary production of algal communities in Dry Valley streams in Antarctica: Examination of the functional role of biodiversity0 aSpecies composition and primary production of algal communities  bBalkema Press, Rotterdam a171-1791 aNiyogi, Dev, K.1 aTate, Cathy, M.1 aMcKnight, Diane, M.1 aDuff, John, H.1 aAlger, Alexander, S.1 aLyons, Berry1 aHoward-Williams, Clive1 aHawes, Ian uhttps://mcm.lternet.edu/content/species-composition-and-primary-production-algal-communities-dry-valley-streams-antarctica00641nas a2200169 4500008004100000245010500041210006900146300001200215490000800227100002400235700001700259700001400276700001900290700002200309700001700331856012300348 1996 eng d00aDetermination of major element chemistry in terrestrial waters from Antarctica by ion chromatography0 aDetermination of major element chemistry in terrestrial waters f a257-2630 v7391 aWelch, Kathleen, A.1 aLyons, Berry1 aGraham, E1 aNeumann, Klaus1 aThomas, James, M.1 aMikesell, D. uhttps://mcm.lternet.edu/content/determination-major-element-chemistry-terrestrial-waters-antarctica-ion-chromatography00527nas a2200133 4500008004100000245008300041210006900124300001400193490000700207100002100214700002400235700002700259856010700286 1996 eng d00aExtreme Supersaturation of Nitrous Oxide in a Poorly Ventilated Antarctic Lake0 aExtreme Supersaturation of Nitrous Oxide in a Poorly Ventilated  a1544-15510 v411 aPriscu, John, C.1 aDownes, Malcolm, T.1 aMcKay, Christopher, P. uhttps://mcm.lternet.edu/content/extreme-supersaturation-nitrous-oxide-poorly-ventilated-antarctic-lake00553nas a2200145 4500008004100000245008300041210006900124300001200193490000700205100002200212700002400234700002500258700002000283856010400303 1996 eng d00aGeochemistry of aquatic humic substances in the Lake Fryxell Basin, Antarctica0 aGeochemistry of aquatic humic substances in the Lake Fryxell Bas a157-1880 v341 aAiken, George, R.1 aMcKnight, Diane, M.1 aHarnish, Richard, A.1 aWershaw, Robert uhttps://mcm.lternet.edu/content/geochemistry-aquatic-humic-substances-lake-fryxell-basin-antarctica00623nas a2200157 4500008004100000245009800041210006900139300001200208490000700220100002000227700002500247700001800272700002300290700002400313856012800337 1996 eng d00aMicrocosms and Soil Ecology: Critical Linkages Between Field Studies and Modelling Food Webs0 aMicrocosms and Soil Ecology Critical Linkages Between Field Stud a694-7050 v771 aMoore, John, C.1 aDe Ruiter, Peter, C.1 aHunt, William1 aColeman, David, C.1 aFreckman, Diana, W. uhttps://mcm.lternet.edu/content/microcosms-and-soil-ecology-critical-linkages-between-field-studies-and-modelling-food-webs00575nas a2200133 4500008004100000245011800041210006900159300001200228490000700240100002200247700002400269700002200293856012600315 1995 eng d00aThe influence of stream channel characteristics on streamflow and annual water budgets for lakes in Taylor Valley0 ainfluence of stream channel characteristics on streamflow and an a284-2870 v301 aHouse, Harold, R.1 aMcKnight, Diane, M.1 aVon Guerard, Paul uhttps://mcm.lternet.edu/content/influence-stream-channel-characteristics-streamflow-and-annual-water-budgets-lakes-taylor00539nas a2200121 4500008004100000245012300041210006900164300001200233490000700245100002400252700002000276856012100296 1995 eng d00aMcMurdo LTER: Algal mat distribution in glacial meltwater streams in Taylor Valley, southern Victoria Land, Antarctica0 aMcMurdo LTER Algal mat distribution in glacial meltwater streams a287-2890 v301 aMcKnight, Diane, M.1 aTate, Cathy, M. uhttps://mcm.lternet.edu/content/mcmurdo-lter-algal-mat-distribution-glacial-meltwater-streams-taylor-valley-southern00613nas a2200145 4500008004100000245011900041210006900160300001200229490000700241100002500248700002500273700002100298700002400319856012400343 1995 eng d00aMcMurdo LTER: Species composition and spatial distribution of algal mats in Green Creek, Taylor Valley, Antarctica0 aMcMurdo LTER Species composition and spatial distribution of alg a289-2910 v301 aAlger, Alexander, S.1 aSpaulding, Sarah, A.1 aShupe, Gordon, H1 aMcKnight, Diane, M. uhttps://mcm.lternet.edu/content/mcmurdo-lter-species-composition-and-spatial-distribution-algal-mats-green-creek-taylor00383nas a2200145 4500008004100000245002300041210002300064300001200087490000700099100002400106700001500130700002700145700001300172856005200185 1995 eng d00aPaleolakes on Mars0 aPaleolakes on Mars a267-2830 v131 aWharton, Robert, A.1 aCrosby, J.1 aMcKay, Christopher, P.1 aRice, J. uhttps://mcm.lternet.edu/content/paleolakes-mars00655nas a2200133 4500008004100000245017000041210006900211100002200280700002400302700002500326700002000351700002400371856012600395 1995 eng d00aStreamflow, water-temperature, and specific-conductance data for selected streams draining into Lake Fryxell, Lower Taylor Valley, Victoria Land, Antarctica, 1990-920 aStreamflow watertemperature and specificconductance data for sel1 aVon Guerard, Paul1 aMcKnight, Diane, M.1 aHarnish, Richard, A.1 aGartner, J., W.1 aAndrews, Edmund, D. uhttps://mcm.lternet.edu/content/streamflow-water-temperature-and-specific-conductance-data-selected-streams-draining-lake00543nas a2200169 4500008004100000245005500041210005400096260001200150300001400162490000700176653001100183100002400194700002400218700002500242700002200267856008400289 1994 eng d00aAquatic fulvic acids in algal-rich antarctic ponds0 aAquatic fulvic acids in algalrich antarctic ponds c07/1994 a1972-19790 v3910aBiggie1 aMcKnight, Diane, M.1 aAndrews, Edmund, D.1 aSpaulding, Sarah, A.1 aAiken, George, R. uhttps://mcm.lternet.edu/content/aquatic-fulvic-acids-algal-rich-antarctic-ponds00600nas a2200157 4500008004100000245008800041210006900129260001200198300001600210490000700226100002700233700001900260700002300279700002400302856011600326 1994 eng d00aLight transmission and reflection in perennially ice-covered Lake Hoare, Antarctica0 aLight transmission and reflection in perennially icecovered Lake c06/1994 a20427-204440 v991 aMcKay, Christopher, P.1 aClow, Gary, D.1 aAndersen, Dale, T.1 aWharton, Robert, A. uhttps://mcm.lternet.edu/content/light-transmission-and-reflection-perennially-ice-covered-lake-hoare-antarctica00688nas a2200181 4500008004100000245010100041210006900142300001200211490000700223100002400230700001700254700002100271700002000292700002400312700002200336700002400358856012400382 1994 eng d00aMcMurdo LTER: Inorganic geochemical studies with special reference to calcium carbonate dynamics0 aMcMurdo LTER Inorganic geochemical studies with special referenc a237-2390 v291 aWelch, Kathleen, A.1 aLyons, Berry1 aPriscu, John, C.1 aEdwards, R., L.1 aMcKnight, Diane, M.1 aHouse, Harold, R.1 aWharton, Robert, A. uhttps://mcm.lternet.edu/content/mcmurdo-lter-inorganic-geochemical-studies-special-reference-calcium-carbonate-dynamics00513nas a2200121 4500008004100000245009500041210006900136300001200205490000700217100002400224700002400248856011900272 1994 eng d00aMcMurdo LTER: Primary production model of benthic microbial mats in Lake Hoare, Antarctica0 aMcMurdo LTER Primary production model of benthic microbial mats  a241-2430 v291 aMoorhead, Daryl, L.1 aWharton, Robert, A. uhttps://mcm.lternet.edu/content/mcmurdo-lter-primary-production-model-benthic-microbial-mats-lake-hoare-antarctica00465nas a2200133 4500008004100000245005900041210005800100300001200158490000700170100002400177700002200201700002100223856008700244 1994 eng d00aMcMurdo LTER: Streamflow Measurements in Taylor Valley0 aMcMurdo LTER Streamflow Measurements in Taylor Valley a230-2320 v291 aMcKnight, Diane, M.1 aHouse, Harold, R.1 aVon Gerard, Paul uhttps://mcm.lternet.edu/content/mcmurdo-lter-streamflow-measurements-taylor-valley00576nas a2200145 4500008004100000245009000041210006900131300001200200490000700212100002500219700002400244700002300268700002100291856011800312 1994 eng d00aPhytoplankton population dynamics in perennially ice-covered Lake Fryxell, Antarctica0 aPhytoplankton population dynamics in perennially icecovered Lake a527-5410 v161 aSpaulding, Sarah, A.1 aMcKnight, Diane, M.1 aSmith, Richard, L.1 aDufford, Richard uhttps://mcm.lternet.edu/content/phytoplankton-population-dynamics-perennially-ice-covered-lake-fryxell-antarctica00402nas a2200121 4500008004100000245004100041210004100082260002400123100002400147700002500171700001400196856007000210 1994 eng d00aStromatolite mats in Antarctic lakes0 aStromatolite mats in Antarctic lakes bKluwer, Netherlands1 aWharton, Robert, A.1 aBertrand-Sarfati, J.1 aMonty, C. uhttps://mcm.lternet.edu/content/stromatolite-mats-antarctic-lakes00696nas a2200157 4500008004100000245017100041210006900212300001200281490000700293100002400300700002200324700002400346700001900370700002500389856012400414 1993 eng d00aDissolved Organic Material in Dry Valley Lakes: A Comparison of Lake Fryxell, Lake Hoare, and Lake Vanda, in Physical and Biogeochemical Processes in Antarctic Lakes0 aDissolved Organic Material in Dry Valley Lakes A Comparison of L a119-1330 v591 aMcKnight, Diane, M.1 aAiken, George, R.1 aAndrews, Edmund, D.1 aBowles, E., C.1 aHarnish, Richard, A. uhttps://mcm.lternet.edu/content/dissolved-organic-material-dry-valley-lakes-comparison-lake-fryxell-lake-hoare-and-lake00588nas a2200121 4500008004100000245016800041210006900209300001200278490000700290100001700297700002300314856012900337 1993 eng d00aThe geochemical evolution of terrestrial waters in the antarctic: the role of rock-water interactions, in Physical and Biogeochemical Processes in Antarctic Lakes0 ageochemical evolution of terrestrial waters in the antarctic the a135-1430 v591 aLyons, Berry1 aMayewski, Paul, A. uhttps://mcm.lternet.edu/content/geochemical-evolution-terrestrial-waters-antarctic-role-rock-water-interactions-physical-and00617nas a2200145 4500008004100000245013500041210006900176300001000245490000700255100002400262700002700286700001900313700002300332856011600355 1993 eng d00aPerennial ice covers and their influence on antarctic lake ecosystems, in Physical and Biogeochemical Processes in Antarctic Lakes0 aPerennial ice covers and their influence on antarctic lake ecosy a53-700 v591 aWharton, Robert, A.1 aMcKay, Christopher, P.1 aClow, Gary, D.1 aAndersen, Dale, T. uhttps://mcm.lternet.edu/content/perennial-ice-covers-and-their-influence-antarctic-lake-ecosystems-physical-and00546nas a2200121 4500008004100000245012000041210006900161300001200230490000700242100002400249700002400273856012700297 1993 eng d00aPotential hydrologic and geochemical consequences of the 1992 merging of Lake Chad with Lake Hoare in Taylor Valley0 aPotential hydrologic and geochemical consequences of the 1992 me a249-2510 v281 aMcKnight, Diane, M.1 aAndrews, Edmund, D. uhttps://mcm.lternet.edu/content/potential-hydrologic-and-geochemical-consequences-1992-merging-lake-chad-lake-hoare-taylor00557nas a2200133 4500008004100000245010300041210006900144300001200213490000800225100002400233700001700257700002200274856012700296 1993 eng d00aStable isotopic biogeochemistry of carbon and nitrogen in a perennially ice-covered Antarctic lake0 aStable isotopic biogeochemistry of carbon and nitrogen in a pere a159-1720 v1071 aWharton, Robert, A.1 aLyons, Berry1 aMarais, D.J., Des uhttps://mcm.lternet.edu/content/stable-isotopic-biogeochemistry-carbon-and-nitrogen-perennially-ice-covered-antarctic-lake