Byron J. AdamsDiana H. Wall 2014-11-13 Soil biota data from the Pulse-Press Project (P3), McMurdo Dry Valleys, Antarctica (2010-2020, ongoing) tabular digitial data McMurdo Dry Valleys LTER McMurdo Dry Valleys LTER 10.6073/pasta/4e965fbbea4e6c3cad25fc044ded333d https://mcm.lternet.edu/content/pulse-press-project-p3-2011 Climate warming in polar regions is associated with thawing of permafrost, resulting in significant changes in soil hydrology, biogeochemical cycling, and in the activity and composition of soil communities. While ongoing, directional climate warming can elicit such responses over decadal time scales, their manifestation typically occurs as discrete thawing pulses. Indeed, in the McMurdo Dry Valleys of Antarctica abrupt changes in community structure and biogeochemical cycling in terrestrial and aquatic ecosystems following a summer warming event (Jan. 2002) exceeded the influences of a decadal cooling trend in both magnitude and rate of response. Thus, we anticipate that climate-mediated permafrost changes and their associated impacts on soil communities and biogeochemical cycles may occur over seasonal time scales. Our objective is to simulate different frequencies of permafrost thawing events in Antarctic permafrost soils. Since the top horizons of most Antarctic soils are dry permafrost (i.e., there is insufficient water content to generate ice-cement), with ice-cement or massive ice typically below 30 cm, permafrost thawing events are likely to result in subsurface movements of water that may manifest as groundwater seeps down gradient. Data contained in these files has been subjected to quality control standards imposed by the investigator. The user of this data should be aware that, while efforts have been taken to ensure that these data are of the highest quality, there is no guarantee of perfection for the data contained herein and the possibility of errors exists. If you encounter questionable data, please contact the MCM LTER data manager corrected or qualified. Thus, these data may be modified and future data will be appended. 2010-01-02 2020-01-04 ground condition This table and EML was originally created by Inigo San Gil in April 2014 with data handed by Michelle Haddix, with Diana Wall at NREL          CONTROL PLOT data issues CONTROL PLOT maintenance start timeend timesensor(s)problemexplanation timeaction  1/17/2013  14:30:00 PMall sensorsdata logger programming errorprogramming error led to randomly distributed errors data before 1/17/2013 requires extensive revision 1/17/13new and improved program uploaded to CR1000, v2.2 9/26/13 7:301/7/14 12:00all sensorsno data battery failure 1/7/14 11:21battery replaced, 5-min v2.2 program uploaded to data logger 1/29/14 12:0012/1/14 12:00all sensorsno databattery failure 12/1/14 11:12battery replaced, 10 min v2.2 program uploaded to data logger PULSE PLOT data issues PULSE PLOT maintenance start timeend timesensor(s)problemexplanation timeaction  1/17/13 12:30all sensorsdata logger programming errorprogramming error led to randomly distributed errors data before 1/17/2013 requires extensive revision 1/17/13new and improved program uploaded to CR1000, v2.2 8/18/13 14:009/24/13 14:00all sensorsno databattery failure 1/7/14 11:47battery replaced and 5-min data collection program uploaded 10/4/13 0:301/7/14 11:30all sensorsno databattery failure 1/9/14PULSE plot wattered - get details from Mike, Jeb, and Byron 4/25/14 18:0010/21/14 12:00all sensorsno/sparse databattery failure 1/8-9/2014 12:00:00 AMexperimental watering of plot - get time from Mike…  PRESS PLOT data issues start timeend timesensor(s)problemexplanation  1/17/13 14:00all sensorsdata logger programming errorprogramming error led to randomly distributed errors data before 1/17/2013 requires extensive revision PRESS PLOT maintenance 9/13/13 12:001/2/14 20:30all sensorsno databattery failure 1/17/13new and improved program uploaded to CR1000, v2.2 4/9/14 12:0010/11/14 10:00all sensorsno databattery failure 1/7/14 12:005-min v2.2 program uploaded to data logger 10/25/14 23:0011/18/14 14:00all sensorsno databattery failure 1/8/14 13:32experimental watering of plot 1/17/13 14:001/1/15 12:00c2_14 sm/tempno datasensor outage 12/1/14 11:30battery replaced 1/9/14 13:15not yet resolvedc15_20 sm/tempno datasensor outage 1/17/13 14:009/13/13 12:00c7_18 sm/tempsparse datasensor malfunction leading to very sparse data collection   As needed A pond, 0.3 nautical miles (0.6 km) long, located 0.5 nautical miles (1 km) south of the snout of Commonwealth Glacier in Taylor Valley, Victoria Land, Antarctica. The pond is part of the Aiken Creek system and receives drainage from several glaciers including Commonwealth Glacier, Wales Glacier and the unnamed glacier next westward. 163.328353881836 163.313079833984 -77.597686767578 -77.598609924316 20m 20m meter This is the plot C, corresponding to the Pulse-Press experiment 163.319885253906 163.319885253906 -77.597579956055 -77.597579956055  This is the plot H, corresponding to the Pulse-Press experiment   163.322067260742 163.322067260742 -77.597396850586 -77.597396850586 This is the plot L, corresponding to the Pulse-Press experiment   163.321914672852 163.321914672852 -77.597610473633 -77.597610473633 LTER Core Areas disturbance None <cntperp> <cntper>McMurdo Dry Valleys LTER Information Manager</cntper> </cntperp> <cntemail>im@mcmlter.org</cntemail> Name: Renée F. Brown Role: data manager Not Applicable Not Applicable Field and/or Lab Methods Field Logs2011-2012LOG: This file created on 12-19-2011 by Martijn Vandegehuchte.Data entered by Martijn Vandegehuchte.Data checked by Martijn Vandegehuchte.Calculations by Martijn Vandegehuchte using the formula: (# individuals / dry soil) * 1000Numbers per kg dry soil adjusted by MLV using formula 1000*(#individuals/(extraction mass*(mass of dry soil/mass of soil used for moisture calc)))Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 11/26/132012-2013LOG: This file created on January 16,2013 by Kevin GeyerData entered by Kevin Geyer.Data checked by Sabrina Saurey.Calculations by Martijn Vandegehuchte using the formula:1000*(#individuals/(extraction mass*(mass of dry soil/mass of soil used for moisture calc)))Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 11/13/132013-2014LOG: This file created on 6 JAN by Matt KnoxData entered by Matt KnoxData checked by Ruth HeindelCalculations by Matt Knox using the formula: (# individuals / dry soil) * 1000Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 10/03/14 We designed a long-term water diversion experiment introducing two frequencies of water additions from a natural pond to trenches excavated to the depth of ice-cement. In Dec. 2011 we established three sets of permanent plots (7.5 X 15m) on the south-facing hillslope above Many Glaciers Pond in Taylor Valley. High-resolution LIDAR imaging, instrumentation, and comprehensive pre-treatment sampling of all plots was conducted in Dec. of 2011, and in Jan. 2013, prior to an additional round of pre-treatment sampling and then initiation of the experiment anticipated in Jan. 2014. Instrumentation consists of thermocouples, delta-T moisture probes and water activity probes buried at multiple active-layer depths every 2 m down-gradient from the water-addition trenches. The three different plots will receive the following different treatments:PRESS Treatment (H): Annual water additions sufficient to raise soil water to 10% water content (as observed during the melt event in Jan. 2002). This is achieved by pumping water from Many Glaciers Pond to a holding tank above the experimental plots, from which we will apply water by gravity feed to a trench during the warmest week of the year (typically the 1st or 2nd week of Jan.).PULSE Treatment (L): Same as above, but water diversions added only in alternate years to simulate a lower frequency of thawing permafrostPositive Control (C): Trenched and instrumented, but no water additionsSampling campaigns consist of 84 soil samples (28 per treatment) collected from permanent plots for quantification of soil biota (invertebrates determined by microscopy and bacteria by 16S rRNA sequencing) and intensive geochemical analyses. This experiment will address the overarching hypothesis: Climate warming in the McMurdo Dry Valleys will amplify connectivity among landscape units leading to enhanced coupling of nutrient cycles across landscapes, and increased biodiversity and productivity. Soil samples were taken for organism enumeration and moisture content analysis as follows: Sampling bags were prepared with one sterile Whirlpak bag and clean plastic scoop per sample. Samples were taken from within the 1m2 area of each plot. The location of the sampling was recorded each year so that areas were not re-sampled. Using the plastic scoop, soil was collected to 10 cm depth. Very large rocks (more than 20 mm diameter) were excluded from the sample. The soil was shoveled into the Whirlpak bag until three quarters full (about 1.5 kg soil). The soil was mixed well in the bag, then the bag was closed tightly, expelling as much air as possible. The soil samples were stored in a cooler for transportation. On return to the laboratory (within 8 hours of sampling), the soils were stored at +4C until further processing. In the laboratory, soil samples were handled in a laminar flow hood to prevent contamination. The Whirlpak bags of soil were mixed thoroughly prior to opening. A sub-sample of approximately 50g was removed and placed in a pre-weighed aluminum soil can, and weighed on a balance accurate to 0.01g. This sample was dried at 105C for 24 hours. The sample was removed, placed in a desiccator to cool down, and re-weighed. These data were used to calculate water content of the soil. The remaining soil in the plastic beaker was weighed. Cold tap water was added up to 650 mL. The soil suspension was stirred carefully (star stir or figure of 8) for 30 seconds, using a spatula. Immediately the liquid was poured into wet screens - a stack of 40 mesh on top of a 400 mesh. The screens were rinsed gently with ice cold tap water (from a wash bottle) through the top of the stack, keeping the screens at an angle as the water filtered through. The water was kept on ice at all times. The top screen was removed, and the lower screen rinsed top down, never directly on top of the soil, but at the top of the screen and from behind. The water was allowed to cascade down and carry the particles into the bottom wedge of the angled screen. The side of the screen was tapped gently to filter all the water through. The suspension was rinsed from the front and the back, keeping the screen at an angle and not allowing the water to overflow the edge of the screen. The soil particles were backwashed into a 50mL plastic centrifuge tube, tipping the screen into the funnel above the tube and rinsing the funnel gently. The suspension was centrifuged for five minutes at 1744 RPM. The liquid was decanted, leaving a few mL on top of the soil particles. The tube was filled with sucrose solution (454g sucrose per liter of tap water, kept refrigerated) up to 45mL. This was stirred gently with a spatula until the pellet was broken up and suspended. The suspension was centrifuged for one minute at 1744 RPM, decanted into a wet 500 mesh screen, rinsed well with ice cold tap water and backwashed into a centrifuge tube. Samples were refrigerated at 4C until counted. Samples were washed in to a counting dish and examined under a microscope at x10 or x2 0 magnification. Rotifers and tardigrades were identified and counted. Nematodes were identified to species and sex, and counted. Total numbers in each sample were recorded on data sheets. All species of nematode, and all rotifers and tardigrades found in the sample were recorded. Data were entered in to Excel files, printed, and checked for errors. Extraction of chlorophyll from the soil. All procedures were carried out in the dark or very low irradiance to avoid degradation of the chlorophyll. The soil samples were mixed thoroughly in the vials, and a sample of approximately 5 g was weighed out in to a 50 mL plastic centrifuge tube with a screw-top cap. 10 mL of a 50:50 DMSO/90percent acetone solution was added to each sample and they were mixed thoroughly on a bench-top Vortex mixer for about 5 seconds. The vials were placed in a -4C constant temperature room, in the dark, and left for 12-18 hours. Determination of chlorophyll a concentration. This was determined fluorometrically using a Turner model 111 fluorometer. A calibration using a known concentration of chlorophyll was carried out prior to sample analysis. The machine was blanked using a 50:50 DMSO/90percent acetone solution. Each vial was mixed thoroughly, then centrifuged for 5 minutes at about 1800 RPM. A sample of approximately 4mL of the DMSO/acetone solution was taken from the top of the sample with a pipette, being careful not to get any soil particles in the solution. The sample was placed in a cuvette, in to the fluorometer and the fluorescence was recorded. This was done fairly quickly in order to prevent light from breaking down the chlorophyll. This measurement is called Fo, the initial fluorescence. After taking this reading, 0.1 mL of 1N HCl was added directly to the cuvette and the cuvette was gently agitated. After 20 seconds, the fluorescence was re-measured. (During this step, the acid converts the chlorophyll to phaeophytin by releasing a magnesium ion in an acidic environment). This measurement is called Fa, the fluorescence after acidification. The solution was discarded in to a waste container, and the cuvette rinsed 3 times with DMSO/90percent acetone solution before proceeding with the next sample. Data were entered in to Excel files, printed, and checked for errors. For measurements of pH, 40 of DI water was added to 20g of soil in a clean, DI- rinsed glass beaker (coarse fragments greater than 2 mm were removed). The samples were stirred until thoroughly mixed (about 5-10 sec). After sitting to equilibrate for 10 minutes the samples were stirred again and a reading was taken with a Beckman 0265 pH meter. For measurements of electrical conductivity, an additional 60 ml of DI water was then added (totaling 100 ml water). The samples were stirred until thoroughly mixed (about 5-10 sec). After sitting to equilibrate for 10 minutes the samples were stirred again and a reading was taken with a YSI 30 conductivity meter. Experimental wetting project, temperature and soil moisture data methodologyThe 3 experimental plots (Press, Pulse, and Control) are instrumented with a network of soil moisture and temperature sensors. Sensors are positioned in vertical nests at 13 locations along the hillslope at each site. Decagon 5TM Soil Moisture and temperature sensors are used to measure Volumetric water content (unitless) and temperature (degrees celcius) at many locations, while thermocouple wires measure temperature (degrees celcius) at other locations. Decagon 5TM Soil Moisture and Temperature Seonsors are wired into a Campbell Scientific AM 16/32B multiplexor, while thermocouple wires are wired into an Campbell Scientific AM25T multiplexor. Both multiplexors are controlled by a Campbell Scientific CR1000 data logger. Each site contains its own multiplexor/data logger array. A site-specific data collection program is uploaded to the CR1000 data loggers at each site. The data collection frequency has changed several times over the life-span of the project, ranging 5-min to 1-hour collection intervals.  Field Logs2011-2012LOG: This file created on 12-19-2011 by Martijn Vandegehuchte.Data entered by Martijn Vandegehuchte.Data checked by Martijn Vandegehuchte.Calculations by Martijn Vandegehuchte using the formula: (# individuals / dry soil) * 1000Numbers per kg dry soil adjusted by MLV using formula 1000*(#individuals/(extraction mass*(mass of dry soil/mass of soil used for moisture calc)))Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 11/26/132012-2013LOG: This file created on January 16,2013 by Kevin GeyerData entered by Kevin Geyer.Data checked by Sabrina Saurey.Calculations by Martijn Vandegehuchte using the formula:1000*(#individuals/(extraction mass*(mass of dry soil/mass of soil used for moisture calc)))Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 11/13/132013-2014LOG: This file created on 6 JAN by Matt KnoxData entered by Matt KnoxData checked by Ruth HeindelCalculations by Matt Knox using the formula: (# individuals / dry soil) * 1000Soil Moisture calculated as the (g of water/ g dry soil) *100 by M L Haddix 10/03/14 unknown SOILS_P3 Dataset Code Unique identifier for the table in the MCM LTER database. In this case, P3. The data provider Unique identifier for the table in the MCM LTER database. In this case, P3. Sample ID A unique sample code consisting of treatment (C, H, or L), block (1, 3, 5, 7, 9, 11, 13), and replicate (a, b, c, d). The data provider A unique sample code consisting of treatment (C, H, or L), block (1, 3, 5, 7, 9, 11, 13), and replicate (a, b, c, d). Date Time The date the soil sample was collected from the field. The data provider calendar date/time mm/dd/yyyy gregorian calendar Treatment The basin where the sample was collected (Fryxell, Hoare, Bonney). The data provider The basin where the sample was collected (Fryxell, Hoare, Bonney). Block Id The block number where the soil sample was collected. Values indicate the distance in meters from the water application trench. 1=1m, 3=3m, and so forth. The data provider The block number where the soil sample was collected. Values indicate the distance in meters from the water application trench. 1=1m, 3=3m, and so forth. Soil Water Content The gravimetric soil water content calculated as a percentage of grams of water divided by the grams of oven dried soil. The data provider 0 100 percent g/g Scottnema Male Live The total number of living male Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Scottnema Male Dead The total number of dead male Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Scottnema Female Live The total number of living female Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Scottnema Female Dead The total number of dead female Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Scottnema Juvenile Live The total number of living juvenile Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Scottnema Juvenile Dead The total number of dead juvenile Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Scottnema Adult The total number of living and dead Scottnema lindsayae adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Scottnema Juvenile The total number of living and dead Scottnema juvenile nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Scottnema Total Live Total number of living Scottnema lindsayae (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Scottnema Total Dead The total number of dead Scottnema lindsayae (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Total Scottnema Live and Dead The total number of live + dead Scottnema lindsayae (juvenile and adult) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Eudorylaimus Male Live The total number of living male Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Eudorylaimus Male Dead The total number of dead male Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Eudorylaimus Female Live The total number of living female Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Eudorylaimus Female Dead The total number of dead female Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Eudorylaimus Juvenile Live The total number of living juvenile Eudorylaimus spp. nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Eudorylaimus Juvenile Dead The total number of dead juvenile Eudorylaimus spp. nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Eudorylaimus Adult The total number of living and dead Eudorylaimus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Eudorylaimus Juvenile The total number of living and dead Eudorylaimus spp. juvenile nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Total Eudorylaimus Live Total number of living Eudorylaimus spp. (juvenile and adult) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Total Eudorylaimus Dead The total number of dead Eudorylaimus spp. (juvenile and adult) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Total Eudorylaimus Live and Dead The total number of live + dead Eudorylaimus spp. (juvenile and adult) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Plectus Female Live The total number of living female Plectus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Plectus Female Dead The total number of dead female Plectus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Plectus Juvenile Live The total number of living juvenile Plectus spp. nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Plectus Juvenile Dead The total number of dead juvenile Plectus spp. nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Plectus Adult The total number of living and dead Plectus spp. adult nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Plectus Juvenile The total number of living and dead Plectus spp. juvenile nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Total Plectus Live Total number of living Plectus spp. (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Total Plectus Dead The total number of dead Plectus spp. (juvenile and adults) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent The data provider 0 # kg/soil Total Plectus Live and Dead The total number of live + dead Plectus spp. (juvenile and adult) nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Total Rotifers Total number of rotifers per kg soil oven dry weight equivalent present in a sample. The data provider 0 # kg/soil Total Tardigrades Total number of tardigrades per kg soil oven dry weight equivalent present in a sample. The data provider 0 # kg/soil Total Living Nematodes The total number of living nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent (#/kg soil). The data provider 0 # kg/soil Total Dead Nematodes The total number of dead nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent (#/kg soil). The data provider 0 # kg/soil Total Living and Dead Nematodes The total number of living and dead nematodes extracted from the soil sample in number of organisms per kg soil oven dry weight equivalent. The data provider 0 # kg/soil Comments Comments about the sample. The data provider Comments about the sample. McMurdo Dry Valleys LTER The data distributor shall not be liable for innacuracies in the content http 1 0 1 column , https://mcm.lternet.edu/sites/default/files/data/mcmlter-soil-p3-biota-20220318_1.csv None 2014-11-13 2014-11-13 McMurdo Dry Valleys LTER http://mcmlter.org/ Biological Data Profile of the Content Standards for Digital Geospatial Metadata devised by the Federal Geographic Data Committee. Drupal Ecological information Management Systems, version D7, Biological Data Profile module